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Visualization of exocellular lectins in the entomopathogenic fungus Conidiobolus obscurus.

We demonstrated the presence of lectins binding to glucose and N-acetylglucosamine on the surface of Conidiobolus obscurus spores by using glycosylated serum albumins substituted with fluorescent dyes and colloidal gold. The role of these exocellular lectins was examined in relation to the adhesion of the fungal spores to their host insect and the pathogenicity of the fungus.

Acetylglucosamine↗

Entomophthorales infection of the maxillofacial region.

Phycomycosis infections caused by the fungi Rhizopus and Mucor are commonly termed mucormycosis. Mucormycosis infections exhibit a rapidly progressive course characterized by angioinvasion and necrosis. The rhinocerebral form has been described thoroughly in the literature. However, there exists another order of Phycomycetes, the Entomophthorales, which rarely cause clinical disease. Their infection is indolent and is associated with granulomatous inflammation and a protracted clinical course. Presented here is a rare case of suspected Canidiobolus coronato infection occurring in the maxillofacial region of a previously healthy male. The authors believe this to be the first reported case occurring in the United States. Because of the poorly documented natural history of this infection and the patient's relative intolerance to parenteral antifungal therapy, a multidisciplinary therapeutic approach was designed. It consisted of long-term, low-dosage amphotericin-B, hyperbaric oxygen and sequential surgical debridements. This unusual disease entity must now be considered in the differential diagnosis of granulomatous disorders of the head and neck.

Amphotericin B↗

[Intestinal entomothoramycosis: report of a case].

A case of intestinal entomophthoramycosis caused by Entomophthorales in a man with 19 years-old, farmer and without associated disease. The patient was submitted to a intestinal resection and diagnosis was carried through after analysis of the surgical specimen. After a review of the literature, the clinical evolution, clinico-pathologic features, difficulties in diagnosis and treatment are discussed.

Adult↗

Rhinofacial zygomycosis caused by Conidiobolus coronatus: a case report including in vitro sensitivity to antimycotic agents.

A case of zygomycosis caused by Conidiobolus coronatus, occurring in a 31-year-old priest from the Ivory Coast, is reported. Lesions eventually resolved after 2 1/2 years, during which the patient was variously treated with intravenous amphotericin B and miconazole as well as surgical resection. It is difficult to be certain which, if any, of these treatments contributed to resolution of lesions. A report of in vitro sensitivity data to a variety of antifungal agents is included.

Adult↗

Disseminated infection caused by Cunninghamella bertholletiae in a patient with beta-thalassemia. Case report and review of the literature.

Rare cases of acute mucormycosis in immunocompromised persons have been attributed to Cunninghamella bertholletiae. We report the first known case of this organism causing a chronic, indolent, systemic disease clinically similar to entomophthoramycosis. Cunninghamella bertholletiae is an opportunistic pathogen whose morbidity may be underappreciated because it is usually considered to be a laboratory contaminant.

Adult↗

Infection of adult mosquitoes by the entomopathogenic fungus Erynia conica (Entomophthorales: Entomophthoraceae).

The infection of adult mosquitoes by the entomopathogenic fungus Erynia conica is presented for the first time. Adult Aedes aegypti were exposed to conidial showers from field-collected chaoborid, tipulid and chironomid cadavers for 24 h under conditions of 100% RH and 15 degrees C. Up to 24% of the adults were killed by the mycosis. Cadavers of Ae. aegypti produced conidia that were infective to other adult Ae. aegypti; however, rates of infection were never more than 12%. Nevertheless, Ae. aegypti served as the laboratory host for E. conica via mosquito-to-mosquito serial passages for up to 6 months. Adult Culex restuans were also susceptible to infection by E. conica.

Aedes↗

[5 new cases of entomophthoromycosis observed in Cameroon. Role of immunofluorescence in thr diagnosis].

The authors present five new cases of entomophthoromycosis observed during three years of histopathology in Cameroon. Three classic cases of rhinoentomophthoromycosis. In one case, Conidiobolus coronatus was isolated. Two cases of subcutaneous entomophthoromycosis, without isolation of the germ. In one observation, the clinical and histological aspect suggested mucormycosis post entomophthoromycosis. An immunofluorescence technique showed the Basidiobolus haptosporus to be the real cause.

Adult↗

[Histology of insect infection by entomopathogenic fungi: interest in a new procedure for the study of precocious phases].

Infective and invasive processes of entomopathogenic fungi expected to be used in biological control shall be analysed in detail. Histological and ultrastructural studies are therefore essential. This paper is devoted to the field of light microscopy; its purpose is to show that coating, in a 4% agar solution, the insects infected by a fungus, prior to fixation and embedding, allows the preservation of the fungal structures present on the cuticle, especially the germinating conidia. This is useful when observing the initial stages of infection. The relevance of this coating technique is illustrated with the example of Pea aphid, Acyrthosiphon pisum, infected by Conidiobolus osmodes. This contribution is the first histological study of infective processes of this entomophthoralean fungus in an insect.

Animals↗

Elephantine nose due to rhinoentomophthoromycosis.

Rhinoentomophthoromycosis in an immunocompetent Indian male due to Basidiobolus species resulting in a huge (elephantine) nasal deformity, is reported. The diagnosis was done by demonstration of hyphae in direct tissue smear examination in potassium hydroxide, histopathological examination and by cultural characteristics. He showed an excellent response to oral potassium iodide solution.

Administration, Intranasal↗

[Anatomo-pathologic aspects of phycomycoses. 6 clinical cases observed in Ivory Coast].

Six cases of superficial phycomycosis have been recently demonstrated in Ivory Coast, the total number of cases being therefore 8 in this country. After a brief review of the etiology, clinical studies, treatment, and geographical distribution of the 150 world cases known to-day, the author studies the histological lesions of this hypodermic granulatous inflammation, whose characteristic aspects (polynuclear micro-abcesses, palissade histiocytes, filaments sheathed in an eosinophil precipitate) are easy to recognise. These anatomopathological aspects vary according to the topography, clinical evolution and treatment. The author presents a summary of the only ultrastructure known today. He points out the significance of this anatomo-pathologic study, which shows the evolution of the immunitary reaction and permits a relatively precise pronostic.

Adult↗

[New approaches to phycomycoses (apropos of 2 cases of rhino-entomophthoromycosis)].

It is an uncommon affection (only 100 cases reported), observed primarily in African peri-equatorial zone. The rhinoentomophtoromycoses are caused by a phycomycetous fungus, class of zygomycetes: Conidiobolus coronatus. They are subcutaneomucus mycoses with centro-facial localizations. During the past ten years, three points have been put into light. 1. A true therapeutic revolution thanks to imidazoles. 2. A better knowledge of this disease in which caricatural forms are far form being the most numerous. 3. A socio-cultural African approach better understood, as such a disease is still considered as the result of a transgression or a fault.

Adult↗