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[Structure of the interphase chromatin in the ciliata Bursaria truncatella macronucleus. II. Loop organization of inactive chromatin clumps].

Electron microscopic study of chromatin organization in isolated macronuclei of a ciliate Bursaria truncatella showed macronuclear chromatin to be organized in compact clumps 120--180 nm in diameter linked with each other by one or several chromatin fibres. Macronucleus being dispersed in a solution of low ionic strength, radial loops basically of nucleosomal structure start appearing around chromatin clumps. Long-time dispersing of macronuclear chromatin brings complete decompactization of chromatin clumps into a set of nucleosome fibres. The way the fibres of interphase chromatin are packed in a chromatin clump is discussed.

Cell Nucleus↗

[Changes in the electrolyte composition of the blood and muscles in carp infection with Ichthyophthirius multifiliis (Ciliata, Ophryoglenidae)].

A study of the electrolytic contents of blood serum, erythrocytes and muscles of carp infected with Ichthyophthirius multifiliis was carried out. Whatever the infection intensity, a characteristic increase in the cations K+ and Na+ ratio in the plasma and muscles of infected fishes takes place. The above dependence, which is expressed in the intensification of active ions transport, is associated with mobilization of organism's protective functions.

Animals↗

Regulation of ciliary pattern in Dileptus (Ciliata). I. Sensory cilia and their conversion into locomotor cilia.

The surface of Dileptus contains three different regions: locomotor, oral and sensory. Each region has cilia with a specific structure and arranged in a characteristic pattern. In the morphogenetic situation when a sensory region transforms into a locomotor one, sensory cilia undergo structural changes converting them into locomotor cilia. The evidence for this is that cilia are found in the transforming region with an inner microtubular pattern intermediate between that of sensory and locomotor cilia. There are also changes in distribution of sensory units leading to a pattern characteristic of locomotor cilia. The conversion of sensory cilia into locomotor ones is also confirmed by a complete lack of evidence for resorption of sensory units within the transforming region, although the resorption is usually very easily observed with the transmission electron microscope. Transformation lasts about 5 h after the operation; afterwards locomotor cilia of normal appearance occupy the transformed region. This way of regulation of ciliary pattern has not been previously described. Its most surprising feature is the regulation of inner structure in an already differentiated ciliary unit. Some aspects of mechanisms which could control this kind of pattern regulation, are discussed.

Animals↗

[Morphobiological characteristics of infusoria of the genus Chilodonella (Ciliata: Chlamydodontidae)].

8 species of fishes from fishfarms of central and north--western regions of the USSR were examined. Chilodonella cyprini Moroff, 1902 and Ch. hexastichus Kiernik, 1909 were found. The reproductive process of infusoria was investigated. The data on the susceptibility of hosts of different species and age are given. The variability of Chilodonella depending on localisation and water temperature is shown.

Animals↗

Effect of seed mass on germination and growth in three dominant species in southern Brazilian coastal dunes.

The effect of seed mass on germination and growth was tested in fresh-seeds of Blutaparon portulacoides, Panicum racemosum, and Spartina ciliata, selected at random in southern Brazilian populations. The seed mass varied within a population of the three species. Both B. portulacoides and P. racemosum showed normal frequency distribution of seed mass, while S. ciliata did not. Significant differences were observed in seed germination between large and small seeds of all species. In all species the capacity of seedling elongation was greater in seedlings of large seeds than those of small ones. Relative growth rate of seedlings of P. racemosum and S. ciliata decreased with time in all seed mass size-classes. On the other hand, the relative growth rate of B. portulacoides seedlings increased during the first 40 days. Seed mass is an important biological factor, affecting seed germination, seedling elongation, and growth of these species, and favoring large seeds, specially in areas of active sand accretion like coastal dunes.

Analysis of Variance↗

Experimental reproduction of phytobezoars.

In a series of in virtro experiments (n = 64) using the mature flowers or seeds and pappus hairs of Karoo bushes Chrysocoma ciliata, Dimorphotheca cuneata, Eriocephalus ericoides, Gazania krebsiana and Gnidia polycephala, small phytobezoars were formed from material of E. ericoides, G. krebsiana and G. polycephala either alone or in combinations. The ratio of fibre to liquid used was found to be optimal around 1: 7 and milling of material enhanced bezoar formation. Time and pH did not appear to significantly affect the formation of bezoars. Other factors were either standardised or not studied. In a small in vivo trial, 4 groups of one sheep and one goat each were fed 10% of either C. ciliata, E. ericoides, G. polycephala seeds and pappus hairs, or a mixture of all 3, added to a basal diet of milled lucerne fed for up to 31 d. Small phytobezoars were formed in the goats but not the sheep receiving C. ciliata, and G. polycephala material. It was concluded that phytobezoars could form in goats or sheep eating large quantities of mature flowers or seeds of several Karoo bushes.

Animals↗

Effects of sawdust pollution on the germination of fungal spores in Lagos Lagoon.

Four fungi, Aspergillus flavus, Aspergillus giganteus, Cladosporium oxysporum a and Trichoderma aureoviride, isolated from Lagos Lagoon, were tested for spore germination in aqueous sawdust extract of different hardwood species (Khaya ivorensis, Mitragyna ciliata and Triplochiton scleroxylon). Extracts of M. ciliata were inhibitory to spore germination especially at higher concentrations. Germ tubes of spores decreased in length with increase in extract concentration for C. oxysporum and T. aureoviride, while Aspergillus species showed increase in length up to a peak at 3% sawdust extract concentration, and thereafter germ tube lengths decreased with increasing concentration. Extracts of T. scleroxylon stimulated the spores and percentage germination increased at higher extract concentrations with no significant difference in germ tube length. Similarly, K. ivorensis had a stimulatory effect on spore germination and length of germ tube, especially at higher extract concentrations. Addition of soluble exogenous carbon and nitrogen sources to sawdust extract enhanced spore germination. Apart from A. flavus, which had only 9% germination, all the spores failed to germinate in lagoon water having 21% salinity. The spores were also inhibited by in lagoon water having 21 per thousand salinity. The spores were also inhibited by various inorganic salts, i.e. CaCl(2), KH(2)PO(4), MgSO(4) and NaCl, present in Lagos Lagoon. The results suggest that sawdust pollution causes enrichment of the lagoon, thereby enhancing spore germination.

Journal Article↗

Analgesic activity of some Indian medicinal plants.

In the present study of some of the Indian medicinal plants Sida acuta whole plant (Malvaeae), Stylosanthes fruticosa (whole plant) (Papilionaceae), Toona ciliata (heart wood) (Meliaceao), Bougainvilla spectabilis (leaves) (Nyctaginaceae), Ficus glomerata (bark, leaves) (Moraceae) and Polyalthia longifolia (leaves) (Annonaceae). The different plants were used in folklore medicine in the treatment of toothache and strengthening of gums, anthelmintic, kidney diseases, analgesic, anti-inflammatory, hepatoprotective, antihyperglycaemic, antihyperglycaemic and anticancer. The extract was prepared using powdered material with ethanol, concentrated under vacuo and were evaluated for analgesic activity by analgesiometer at three dose level (100, 300 and 500mg/kg). Analgesic activity was significant with Toona ciliata (heart wood) ethanolic extract when compared with other extracts and its activity was confirmed by tail immersion method.

Analgesics↗

Molecular characterization and distribution of a 145-bp tandem repeat family in the genus Populus.

This report aims to describe the identification and molecular characterization of a 145-bp tandem repeat family that accounts for nearly 1.5% of the Populus genome. Three members of this repeat family were cloned and sequenced from Populus deltoides and P. ciliata. The dimers of the repeat were sequenced in order to confirm the head-to-tail organization of the repeat. Hybridization-based analysis using the 145-bp tandem repeat as a probe on genomic DNA gave rise to ladder patterns which were identified to be a result of methylation and (or) sequence heterogeneity. Analysis of the methylation pattern of the repeat family using methylation-sensitive isoschizomers revealed variable methylation of the C residues and lack of methylation of the A residues. Sequence comparisons between the monomers revealed a high degree of sequence divergence that ranged between 6% and 11% in P. deltoides and between 4.2% and 8.3% in P. ciliata. This indicated the presence of sub-families within the 145-bp tandem family of repeats. Divergence was mainly due to the accumulation of point mutations and was concentrated in the central region of the repeat. The 145-bp tandem repeat family did not show significant homology to known tandem repeats from plants. A short stretch of 36 bp was found to show homology of 66.7% to a centromeric repeat from Chironomus plumosus. Dot-blot analysis and Southern hybridization data revealed the presence of the repeat family in 13 of the 14 Populus species examined. The absence of the 145-bp repeat from P. euphratica suggested that this species is relatively distant from other members of the genus, which correlates with taxonomic classifications. The widespread occurrence of the tandem family in the genus indicated that this family may be of ancient origin.

Base Sequence↗

[Host preference of Culicidae (Diptera) collected in central Argentina].

In order to study the host preference of female mosquitoes, samples were taken fortnightly in Cordoba and Cosquin (Argentina), during October-April of two consecutive years. Four different vertebrates were used in baited-can traps: frogs, chickens, rabbits and turtles. The genus Culex accounted for 92.9% of the specimens collected. Aedes for 7.0% and Psorophora ciliata 0.02%. The highest proportion of females were collected in chicken traps (68.7%), followed by rabbit traps (29.9%), turtles (0.8%) and frogs (0.5%), thus the majority of the mosquitoes were collected in traps with homeotermous hosts. Only Culex dolosus fed on all the hosts. Culex acharistus, Cx. chidesteri and Cx. quinquefasciatus fed on chickens, rabbits and turtles. Aedes albifasciatus, Ae. scapularis, Cx. bidens and Cx. coronator fed on both homeotermous hosts. Culex apicinus, Cx. maxi, Cx. saltanensis and Cx. spinosus fed only on chickens and Ps. ciliata only on rabbits.

Animals↗