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Results for “CEREBROSPINAL FLUID PROTEINS”

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At least 145 records · Page 8Linked to original sources
PubMed1994

Cerebrospinal fluid protein concentrations related to clinical findings in patients with sciatica caused by disk herniation.

The protein concentration in the cerebrospinal fluid (CSF) is often increased in patients with sciatica, probably due to leaking of plasma proteins through the blood-nerve root barrier into CSF. Positive straight leg raising test, paresis, altered sensibility, reduced reflexes, and type of treatment were related to the CSF protein concentrations in 180 patients with sciatica caused by verified lumbar disk herniation. Significantly higher values of the CSF/serum albumin ratio and the CSF/serum immunoglobulin G ratio were found both in patients with positive straight leg raising test results and paresis compared with patients with no clinical findings. In the patients who had undergone emergency surgery, the same ratio parameters were significantly higher when compared with those who had undergone routine surgery and those had not undergone surgery. Elevated CSF proteins seem to be important indicators of the functional status of the nerve root and a measure of the degree of seriousness of sciatica. The interaction of smoking on CSF proteins was also studied.

Adult↗
PubMed1984

Cerebrospinal fluid proteins in muscular dystrophy patients.

We analyzed and quantified the microcomponents of protein fractions in the cerebrospinal fluid of patients with various types of muscular dystrophy. The degenerative pattern is characterized by an increase in the prealbumin fraction and a decrease in the gamma-globulin fraction as shown in the Duchenne and congenital muscular dystrophy. The increase in CSF IgG, gamma-globulin fraction, and myelin basic protein is shown in the myotonic dystrophy. In addition to the lowness of IQ, and the abnormality of EEG and brain CT, abnormal CSF proteins obviously suggest the presence of CNS involvement in muscular dystrophy.

Adolescent↗
PubMed1994

Elevated cerebrospinal fluid proteins in sciatica caused by disc herniation.

We carried out a study of cerebrospinal fluid (CSF) proteins in 180 patients with sciatica caused by lumbar disc herniation to elucidate further the degree and mechanisms of protein elevations. The 63 controls were patients with tension headache or migraine without aura. The CSF/serum albumin ratios were higher in the patients (mean 8.84, SD 5.16) than in the controls (mean 5.60, SD 2.33). Similar differences were found for the CSF/serum IgG ratios and the CSF-total proteins. The CSF/serum albumin ratios, CSF/serum IgG ratios and the CSF-total protein concentrations were higher in men than in women among the patients. We suggest that the significant difference in ratio parameters between patients and controls indicates a leak of plasma albumin, most likely IgG, into the CSF in patients with sciatica. The leak was more pronounced in men. Also in the control group the CSF/serum albumin and CSF/serum IgG ratios were higher in men.

Adolescent↗
PubMed1991

Prognostic value of cerebrospinal fluid protein content and leukocyte count in infants and childhood bacterial meningitis.

Seventy infants and children with bacterial meningitis were studied. All children were treated with ampicillin and chloramphenicol. A significant increase in the cerebrospinal fluid protein concentration determined on admission was found in patients who died as compared to those who survived (P less than 0.05). However, no difference was observed between the admission CSF leukocyte count in those patients who died versus those who survived. From this study, it can be concluded that the determination of CSF protein level on admission is an easy, fast and reliable method that can be used to predict clinical outcomes in acute bacterial meningitis.

Adolescent↗
PubMed1977

Crossed immunoelectrofocusing for identification of normal and abnormal cerebrospinal fluid proteins. A preliminary report.

Isoelectric focusing is a valuable method for the analysis of cerebrospinal fluid (CSF) proteins. Its high resolving power has, however, created problems in identifying the large number of protein bands separated. In an attempt to identify these bands, two-dimensional crossed immunoelectrofocusing has been used, where isoelectric focusing is combined with rocket immunoelectrophoresis. By these methods 9 of the normal proteins in the acidic pH interval have been identified. In addition the unusual CSF protein abnormalities occurring in Marie-Sanger-Brown's ataxia and alcoholic cerebellar degeneration have been shown to represent increases of different microheterogeneous forms of transferrin.

Alcoholism↗
PubMed2003

Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease.

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.

Aged↗
PubMed1979

Familial amentia, unusual ventricular calcifications, and increased cerebrospinal fluid protein.

A sibship originally reported by Friedman and Roy as showing severe mental retardation, strabismus, hyperactive tendon reflexes, lalling speech, and foot deformities was restudied. Three major additional findings were noted. The cerebrospinal fluid protein concentration was increased two to three times above normal in four siblings who were available for study. Radiographs of cranial structures in three siblings showed identical pathologic intracranial calcifications which correspond in distribution to the choroid plexus. The choroid plexus was not demonstrable in one patient when radiolabeled 99m-Tc-pertechnetate was injected without perchlorate. Neuropathologic findings in one sibling included small subcortical heterotopias and atrophy of the choroid plexus with encasement by glial fibrils. These findings denote a new heredofamilial neurologic syndrome associated with mental retardation and a disorder of choroid plexus.

Atrophy↗
PubMed1982

A silver staining method for protein bands or immunoprecipitates in electrophoresis or immunoelectrophoretic treatment of unconcentrated cerebrospinal fluid proteins using the commercial agarose plates (Corning Universal System).

A highly sensitive silver staining method for protein bands or immunoprecipitates in electrophoresis or immunoelectrophoresis of unconcentrated cerebrospinal fluid using the commercial agarose plates (Corning Universal System) has been established. The new method is based on the reaction between protein-potassium ferrocyanide complex and silver nitrate as is the conventional silver staining in agar gel electrophoresis. However, some modifications were introduced into the procedures in order to obtain the densitometric values of fractionated proteins coincident with those by the usual amidoblack 10B staining for the same concentrated samples. Furthermore, in immunoelectrophoresis, several minor component proteins which could not be found by the usual staining have been detected in the cerebrospinal fluid samples.

Amido Black↗
PubMed1980

Determination of urine and cerebrospinal fluid protein with the American Monitor KDA.

In turbidimetric measurements, true light absorbance is not measured but rather dispersion of light by particles in suspension causes a decrease in light energy to reach the detector. Reproducible, sensitive results can be obtained by controlling such parameters as timing, temperature, mixing of sample and reagents, and use of decrease wave-length. I have adapted two manual turbidimetric methods for the determination of the total protein in urine and cerebrospinal fluid to the American Monitor KDA. The method in precise and rapid, and correlates well with the two manual methods while using small sample and reagent volumes. The method is linear to at least 5.0 g/l.

Autoanalysis↗