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Heat tolerance of Salmonella enterica serovars Agona, Enteritidis, and Typhimurium in peanut butter.

Recent large foodborne outbreaks caused by Salmonella enterica serovars have been associated with consumption of foods with high fat content and reduced water activity, even though their ingredients usually undergo pasteurization. The present study was focused on the heat tolerance of Salmonella enterica serovars Agona, Enteritidis, and Typhimurium in peanut butter. The Salmonella serovars in the peanut butter were resistant to heat, and even at a temperature as high as 90 degrees C only 3.2-log reduction in CFU was observed. The obtained thermal inactivation curves were upwardly concave, indicating rapid death at the beginning (10 min) followed by lower death rates and an asymptotic tail. The curves fitted the nonlinear Weibull model with beta parameters < 1, indicating that the remaining cells have a lower probability of dying. beta at 70 degrees C (0.40 +/- 0.04) was significantly lower than beta at 80 degrees C (0.73 +/- 0.19) and 90 degrees C (0.69 +/- 0.17). Very little decrease in the viable population (less than 2-log decrease) was noted in cultures that were exposed to a second thermal treatment. Peanut butter is a highly concentrated colloidal suspension of lipid and water in a peanut meal phase. We hypothesized that differences in the local environments of the bacteria, with respect to fat content or water activity, explained the observed distribution and high portion of surviving cells (0.1%, independent of the initial cell number). These results demonstrate that thermal treatments are inadequate to consistently destroy Salmonella in highly contaminated peanut butter and that the pasteurization process cannot be improved significantly by longer treatment or higher temperatures.

Arachis↗

Liquid chromatographic determination of aflatoxin levels in peanut butters using an immunoaffinity column cleanup method: international collaborative trial.

Thirteen laboratories in 7 different countries participated in a collaborative trial to evaluate the immunoaffinity column cleanup procedure with quantitation by fluorescence liquid chromatography (post-column derivatization) for the determination of aflatoxins in peanut butters. Participants were sent 10 randomly numbered samples of roasted peanut butter for analysis (5 pairs of undisclosed duplicates). Two of the pairs were "blank" peanut butters to which aflatoxin standards had been added; these "spiked" samples were used for recovery purposes. The other 3 pairs of samples were a nominal "blank" and 2 naturally contaminated peanut butters. A full statistical presentation of the results is given. Coefficients of variation (CVs) for the total aflatoxin determinations for mean levels of 4, 15, and 38 microns/kg were between 32 and 44% for the blank and 2 trial samples. Recovery levels for the 2 spiked samples were 51-67%, with aflatoxin B1 recovery of 60%. Relative standard deviations for method repeatability (RSDr) and reproductibility (RSDR) for the 3 trial samples were 15-26% and 33-45%, respectively.

Aflatoxins↗

Determination of aflatoxin concentrations in peanut butter by enzyme-linked immunosorbent assay (ELISA): study of three commercial ELISA kits.

Sixteen United Kingdom analytical laboratories participated in an evaluation of 3 commercially available enzyme-linked immunosorbent assay (ELISA) kits for analysis of aflatoxin in peanut butter. Each laboratory was sent 3 sets of 10 randomly numbered samples of peanut butter. Each set consisted of 5 pairs of undisclosed duplicates. Four of the sets of duplicates were naturally contaminated butters with "target" aflatoxin values (estimated by liquid chromatography) between 8 and 81 micrograms/kg. The fifth pair was a blank peanut butter containing approximately 3 micrograms/kg of total aflatoxins. A statistical treatment of the results of the study is presented, together with discussion of the relative merits of the different kits.

Aflatoxins↗

[Immunologic analysis of milk, hen egg, and soybean proteins in butter and margarine, and clinical assessment for availability of hypoallergenic margarine (HAM)].

To determine the allergenic proteins in commercially available butter and margarine, protein fractions were extracted and immunologically analyzed against milk, hen egg, and soybean antigens. Butter and 10 kinds of margarine were proved to contain these proteins in various concentrations by ELISA and immunoblotting methods by use of the rabbit antisera developed against each food proteins. However, hypoallergenic margarine was found to contain no such proteins at all. Using sera obtained from atopic dermatitis patients, previously detected high levels of IgE antibodies to milk, hen egg, or soybeans, the extracted protein fraction from butter and margarine was analyzed whether these proteins react with IgG antibodies in patients' sera. The sera with high levels of specific IgE recognized protein antigens in these extracts except those from hypoallergenic margarine, suggesting that proteins in butter and margarine may become allergenic for the patients sensitive to the proteins, and that hypoallergenic margarine will be a reasonable alternative for the allergic patients to milk, hen egg, or soybeans.

Adolescent↗

A survey on organochlorine pesticide residues in butter and cracked wheat available in Turkish markets.

This study has been conducted with a view to examine the level of organochlorine pesticide residues which are Quintozene (PCNB), Dichlorodiphenyl trichloroethane (p,p'-DDT), Dichlorodiphenyl dichloroethylene (p,p'-DDE) and Dichlorodiphenyl dichloroethane (p,p'-DDD), exiting in butter and cracked wheat sold in Ankara local markets. Thus, the use of some pesticides is restricted due to the factors such as the environmental pollution and for saving the health of consumers. Therefore, the aim of this study is to analyse the results achieved from such a restriction. 100 samples of butter and 50 samples of cracked wheat were obtained from the different markets in order to be analysed. After extracting the organochlorine pesticides from the samples, the amounts have been determined by gas liquid chromatography-ECD method. The results of this study revealed that the butter sold in Ankara local markets did not contain organochlorine pesticide residues while the cracked wheat contained PCNB and lindane residues although the amounts of residues had been found in the Food and Agriculture Organization (FAO) tolerance limits.

Butter↗

Low-fat (41%) butter consumption decreases total energy and lipid intake in diabetic patients under acute conditions.

Decreasing fat intake in subjects at risk of cardiovascular diseases and particularly diabetics is a major issue. To investigate whether low-fat (41%) butter (LFB) is of any benefit compared to regular butter (RB), 97 hospitalized diabetics (41 insulin-dependent) were studied on four consecutive days. Breakfast (bread, butter and drink) was served at 0830 hrs, on successive mornings. LFB and RB were presented ad libitum, on alternate days. Satiety was assessed at 10 and 12 h, using line rating scales. At 1230 hrs lunch was served, with large servings corresponding to 130% of the recommended lunch intake, so that carry-over effects from the breakfast manipulation could be measured. At breakfast, LFB was consumed in higher amounts, 27 vs. 21 g, F(1,96) = 33.24, p < 0.0001, than RB; however, the energy intake was significantly lower (by about -38%) on LFB days, F(1,96) = 158.3, p = 0.0001. Hunger at 10 h but not at 12 h was affected by breakfast conditions. Lunch intake was comparable following LFB and RB breakfasts. In conclusion, LFB utilization under acute conditions seems to benefit diabetics by reducing caloric and fat intake.

Adult↗

Intestinal lymph absorption of butter, corn oil, cod liver oil, menhaden oil, and eicosapentaenoic and docosahexaenoic acid ethyl esters in rats.

Adult male rats were surgically given a drainage catheter in the main mesenteric lymph duct. After an overnight fast, five groups of rats received intragastrically, in one bolus, butter, corn oil (CO), cod liver oil (CLO), menhaden oil (MO), or ethyl esters of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids (K80). Intestinal lymph was collected in these conscious animals, each hour during the first 6 h and in a single sample for the next 18 h. The absorption peak appeared earlier after MO and CO than after CLO administration. The quantities of triglycerides recovered during the first 6 h were significantly lower after butter (91 mg) and K80 (54 mg) administration than for the other three oils. No difference was observed between the vegetable oil and the marine oils (CO = 173 mg, CLO = 148 mg, MO = 180 mg). The total triglyceride recovered in 24 h was highest after CLO (410 mg) and lowest with K80 (146 mg). An increase in the weight percentage of some characteristic fatty acids of the lipid mixtures was observed: oleic acid for butter, oleic and linoleic acids for CO, EPA and DHA for CLO, MO, and K80. Chylomicrons were the largest with CO, more numerous and smaller with CLO, and the smallest with K80. Results obtained illustrated the relation between gastrointestinal hydrolysis, enterocyte biochemical events, and lymph triglyceride absorption profiles as related to the composition and distribution of triglyceride fatty acids.

Animals↗

Cholesterol vehicle in experimental atherosclerosis. Part 15. Randomized butter and randomized lard.

Randomized lard and butter oil were conpared with native lard and butter oil for their effects on cholesterol-induced atherosclerosis in rabbits. In each experiment there was also a group fed corn oil. The diets contained 2% cholesterol and 6% fat and were fed for eight weeks. Randomization of either butter or lard had virtually no effect as regards their atherogenic potential when fed as part of a diet containing 2% cholesterol. The corn oil-containing diet was less atherogenic than any of the other fats.

Animals↗

Organochlorine pesticides in Argentinian butter.

Organochlorine pesticide residues were analysed in 150 samples of butter collected from several places in the cities of Santa Fe and Rosario (Argentine Republic) over a period of 18 months. Pesticides analysed in butter were: HCH (alpha and gamma isomers), heptachlor and its epoxide, aldrin, dieldrin, and DDT (p,p' DDE, o,p' DDT and p,p' DDT). Most samples contained residues of gamma-HCH (Lindane) and heptachlor (92% and 78%, respectively); alpha-HCH and aldrin were detected in 58% and 55% of samples; dieldrin, heptachlor epoxide, DDT isomers were found in few cases (30%). Mean values of pesticides residues in butter fat were: sigma HCH 0.029 ppm; sigma heptachlor 0.064 ppm; sigma aldrin 0.11 ppm and sigma DDT 0.024 ppm. Residue levels exceeded the limits prescribed by the Food and Agricultural Organization/World Health Organization in only very few cases.

Argentina↗

Arachidonic acid and linoleic acid supplementation increase prostanoid production in rats fed a butter-enriched diet.

Male Sprague Dawley rats were fed a butter-enriched diet (50% fat) for 2 weeks and then supplemented orally with either 90 mg of ethyl arachidonate or ethyl linoleate daily for 2 weeks. For comparative reasons, one group of animals was fed standard laboratory rat chow for 4 weeks. Aortic prostacyclin (PGI2) production, platelet aggregation and thromboxane A2 (TXA2) production and plasma and aortic phospholipid (PL) fatty acids were measured. When compared to butter-fed rats, aortic PGI2 production, collagen-induced platelet aggregation and TXA2 production were significantly increased in rats supplemented with ethyl arachidonate to levels similar to those seen in chow-fed rats. Ethyl linoleate supplementation also tended to increase aortic PGI2 production, collagen-induced platelet aggregation and TXA2, but not to the same extent. These changes were accompanied by increases in the level of arachidonic acid and linoleic acid in aortic and plasma PL and a decrease in the level of eicosapentaenoic acid (EPA) and docsahexaenoic acid (DHA). These data indicate that supplementation with small doses of preformed arachidonic acid was more effective than supplementation with its precursor, linoleic acid, in reversing the effects on prostanoid production and phospholipid fatty acid composition in rats fed diets enriched with butter.

Animals↗

Increase of the PCDD/F-contamination of milk, butter and meat samples by use of contaminated citrus pulp.

In Germany from 1993 to 1997, the polychlorinated dibenzo-p-dioxins and -furans (PCDD/F) contamination of food decreased slowly but constantly. However, for milk and butter, this trend was gradually reversed beginning in September 1997: From summer 1997 to February 1998 the average PCDD/F contamination of dairy products increased from a low level of about 0.6 pg I-TEQ/g fat in summer 1997. The dioxin content, of a limited number of randomly collected samples, rose on average to 1.41 pg I-TEQ/g fat (median 1.06 pg I-TEQ/g fat) in different regions of Germany in February 1998. A butter sample from the Netherlands with 1.96 pg I-TEQ/g fat hinted at the same source. The congener pattern in all contaminated milk and butter samples had elevated amounts of 2,3,7,8-TCDD and 1,2,3,7,8-PeCDD. Also, meat samples (beef, cow's meat and veal) with the same dioxin pattern were found to contain between 1.72 and 4.26 pg I-TEQ/g fat (background contamination of 0.53 pg I-TEQ/g fat). Large number of samples were analyzed to find a key for the cause. With a farmer producing milk with about 4.9 pg I-TEQ/g fat, a new source of PCDD/F for food contamination was discovered: the use of PCDD/F contaminated citrus pulp from Brazil as feed material for ruminants on a very large scale. Containing about 5-10 ng I-TEQ/kg, this component was about 20-100 times more highly contaminated than average feed with background contamination. Very complex pieces of circumstantial evidence were gathered to prove the correlation between this component of feed and the increase of dioxin contamination in milk. With respect to the huge trade of this feed ingredient on the global market, many other countries were involved. As an immediate response on these findings, the European Community fixed a preliminary maximum permitted level of 500 pg I-TEQ/kg citrus pulp, valid since August 1998.

Animal Feed↗

Organochlorine pesticide residues in cow's milk and butter in Mexico.

This monitoring study of 355 samples of cow's milk collected from the central region of Veracruz state and 448 samples of national butter brands was conducted to determine the contamination levels of organochlorine pesticides. The results obtained for mean HCH levels were 0.094 and 0.093 mg/kg on fat basis in cow's milk and butter samples, respectively. The mean DDT levels were 0.159 and 0.049 mg/kg, respectively. In relation to cow's milk, the total HCH levels in Veracruz state were higher but total DDT levels were comparable to those reported in other countries. On the other hand, organochlorine levels detected in national brand butter samples were lower than those found in other countries, where these pesticides are still used in sanitary actions. These results confirmed that dairy products in Mexico presented organochlorine pesticide residues (owing to their use in sanitary actions) indicating a human exposure through these food products.

Agriculture↗

Cholesterol oxidation in butter and dairy spread during storage.

In a dairy spread (800 g lipid/kg, 10 g salt/kg) based on 750 g milk fat/kg and 250 g rapeseed oil/kg fat in 15 g extruded catering packaging, there was a more significant accumulation of cholesterol oxidation products than in butter (minimum 800 g lipid/kg, 12 g salt/kg) in 10 g extruded catering packaging when stored at 4 or at 20 degrees C. There was a lag phase of 7 weeks in cholesterol oxidation in dairy spread stored at 4 degrees C, while no lag phase was observed for storage at 20 degrees C. Total concentrations of oxysterols were, however, very similar for dairy spread stored at 4 and 20 degrees C after 13 weeks storage (approximately 12 micrograms/g milk lipid); storage at -18 degrees C almost prevented cholesterol oxidation (approximately 4 micrograms/g milk lipid). For butter, cholesterol oxidation was less pronounced at 4 degrees C (<3 micrograms/g milk lipid) than at -18 degrees C (approximately 4 micrograms/g milk lipid) and 20 degrees C (approximately 7 micrograms/g milk lipid). 7-Ketocholesterol was the dominant oxidation product, with 1.3 and 5.7 micrograms/g milk lipid in butter and dairy spread respectively after 13 weeks storage at 4 degrees C.

Butter↗

Effects of dietary calcium soaps of unsaturated fatty acids on digestion, milk composition and physical properties of butter.

Dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with Ca soaps (CaS) of palm fatty acids (FA) and rapeseed FA. Diets compared were control diet based on maize silage and concentrate, and two diets with 40 g CaS of palm oil FA or rapeseed oil FA/kg diet, replacing part of the concentrates of the control diet. Total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving CaS; the extent of ruminal biohydrogenation of total unsaturated C18 FA was significantly reduced by both CaS diets. Apparent intestinal digestibility of FA was not different among diets, although the amount of FA absorbed with the CaS diets was twice that with the control diet. No difference among diets was observed for milk production, or fat and protein contents. Giving CaS diets decreased the proportions of 4:0 to 14:0 FA in milk fat, and increased cis-18:1n-9, compared with control diet. The rapeseed diet lowered the content of 16:0, and increased the contents of 18:0 and trans-18:1n-7. CaS diets did not result in a marked increase of polyunsaturated FA content in milk fat. Butter from cows fed on the CaS diets contained more liquid fat at 6 and 14 degrees C than butter from the cows fed on the control diet. Incorporating CaS, particularly those from rapeseed, in dairy cows' diets increased C18 FA in milk and improved butter spreadability.

Ammonia↗

Active photosensitizers in butter detected by fluorescence spectroscopy and multivariate curve resolution.

In this study, fluorescence excitation and emission matrices and multivariate curve resolution (PARAFAC) were used to detect and characterize active photosensitizers spectrally in butter. Butter samples were packed under high (air) and low oxygen (<0.05%) atmospheres and exposed to violet, green, or red light. Six photosensitizers were found: riboflavin, protoporphyrin, hematoporphyrin, a chlorophyll a-like molecule, and two unidentified tetrapyrrols. By estimation of relative concentrations, we could follow how each sensitizer was photodegraded as function of wavelength, oxygen level, and time. The degradation rate of protoporphyrin, hematoporphyrin, chlorophyll a, and one of the tetrapyrrols correlated well (0.83-0.91) with the formation of sensory measured oxidation. The results suggest that mainly type I photoreactions were responsible for the degradation of photosensitizers in both high and low oxygen atmosphere. Type II photoreactions (generation of singlet oxygen) were involved in the oxidation of butter stored in air. The study shows that PARAFAC modeling of fluorescence landscapes is an excellent tool for studying photooxidation in complex systems.

Butter↗

Influence of butter and of corn, coconut and fish oils on the effects of recombinant human tumour necrosis factor-alpha in rats.

1. Tumour necrosis factor-alpha is produced in response to inflammatory stimuli. Fish oil can suppress the production and actions of cytokines. Little information is available on the effects of other fats on cytokine biology. We compared the effects of fats, with a wide range of fatty acid characteristics, on the effects of tumour necrosis factor-alpha on protein and zinc metabolism in rats. 2. Weanling rats were fed for 8 weeks on diets containing 10% fat in the form of corn, fish or coconut oils or butter before an intraperitoneal injection of recombinant human tumour necrosis factor-alpha was given. Measurements were made 24h after the injection. 3. In rats fed corn oil, food intake was reduced by 62% and rates of protein synthesis were increased by 86, 32 and 39% in the liver, lung and kidney, respectively. Zinc concentrations increased by 23% in the liver but decreased by 10% in the kidney. Plasma caeruloplasmin and complement C3 levels increased by 25% and 28%, respectively, and plasma albumin level decreased by 24%. 4. Fish oil prevented the increase in hepatic protein synthesis and changed the response of protein synthesis in lung and kidney to a decrease. Changes in hepatic and renal zinc concentrations were prevented. The response of the plasma caeruloplasmin level was unaltered but those of the plasma complement C3 and albumin concentrations were prevented. 5. Coconut oil and butter, although similarly low in linoleic acid, differed in their modulatory effects. With the exception of the rise in the plasma complement C3 concentration, all responses were prevented or greatly inhibited in rats fed butter.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Persistent organochlorine pesticides in Mexican butter.

Organochlorine pesticides have been used in Mexico in malaria control programmes against ectoparasites and as seed dresser. Owing to their chemical stability, they tend to accumulate in the lipid part of the organisms. The stored pesticides are excreted with the endogenous fat during milk production. The aim was to monitor the organochlorine pesticide levels in butter manufactured in Mexico. From the pesticides, only HCB, beta-HCH, pp'-DDT, op'-DDT and pp'-DDE with major frequency and levels were detected. The HCB mean level was low at 0.008 mg kg(-1) on a fat basis. From the HCH isomer, only the beta-HCH at 0.065 mg kg(-1) on a fat basis was determined, remaining as the main contaminant of the monitored butters. Among DDTs, pp'-DDE was the major constituent (0.043 mg kg(-1) on a fat basis) followed by pp'-DDT (0.036 mg kg(-1)) and op'-DDT (0.009 mg kg(-1)). Comparing the previous study (1994) and this one (2001), all organochlorine pesticides had a descendent tendency; beta-HCH decreased from 0.095 to 0.065 mg kg(-1) on a fat basis, whereas the total DDT decreased from 0.056 to 0.047 mg kg(-1), pp'-DDT from 0.050 to 0.036 mg kg(-1), op'-DDT from 0.018 to 0.009 mg kg(-1), while pp'-DDE increased from 0.032 to 0.043 mg kg(-1). The decreased DDT levels in Mexican butters is caused by the substitution of organochlorine insecticides with pyrethroids used by the Mexican Ministry of Health since 1999 in sanitary programmes.

Animals↗

Effect of diets high in butter, corn oil, or high-oleic acid sunflower oil on serum lipids and apolipoproteins in men.

This randomized blind crossover study compared serum lipid and apolipoprotein concentrations in 20 men consuming 37-43% of energy as fat from diets based on corn oil, high-oleic acid sunflower oil, and butter. Each phase of the crossover design included 2 wk of butter-based diet followed by 5 wk of designated vegetable-oil-based diet with a 7-wk washout period between phases. Compared with values for the butter-based diet, the vegetable-oil-based diets reduced serum total cholesterol by 16-21% (p less than 0.001), LDL cholesterol by 21-26% (p less than 0.001), triglycerides by 10-21% (p less than 0.01 for the higher figure), and apolipoprotein B-100 by 22-29% (p less than 0.001). When values fell, they fell further on the corn-oil-based diet. There were no significant changes in serum HDL cholesterol or apolipoprotein A-1. These data suggest that when men on diets high in saturated fatty acids reduce their saturated fatty acid intake but not their total fat intake, many can still experience a significant lowering in serum total cholesterol.

Adult↗