Characteristics of peripolar cell granules of antelopes and goats.
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G- and C-banded karyotypes of four species of the genus Kobus were compared using the standard karyotype of Bos taurus. Chromosomal complements were 2n = 50-54 in K. ellipsiprymnus, 2n = 50 in K. kob, 2n = 48 in K. leche, and 2n = 52 in K. megaceros. The number of autosomal arms in all karyotypes was 58. Fifteen autosomal pairs were conserved among these four species, including the 1;19 and 2;25 centric fusions, and autosomal differences involved eight centric fusion rearrangements. Five centric fusions were each unique to a particular taxon: 3;10 (K. leche), 3;11 and 6;29 (K. kob), and 5;17 and 7;11 (K. ellipsiprymnus). The 4;7 fusion occurred in K. leche and K. megaceros, whereas the 5;13 fusion occurred in K. kob and K. leche; the 6;18 fusion was found in three species but was absent in K. kob. Differences between the X chromosomes of the four Kobus species were attributed to heterochromatic additions or deletions, and Y-chromosome differences may have been the result of pericentric inversion. G-banded karyotypes of putative K. l. leche and K. l. kafuensis appeared identical, as did C-banded karyotypes of the two subspecies. Karyotypes of K. e. ellipsiprymnus and K. e. defassa differed as a result of the 6;18 centric fusion, which was polymorphic in K. e. defassa, and the 7;11 centric fusion, which was polymorphic in K. e. ellipsiprymnus but absent in K. e. defassa. Several centric fusions were related by monobrachial chain-IV complexes; however, records of hybridization indicate that reproductive isolation between at least certain species of Kobus is incomplete. Karyotypic differences between K. ellipsiprymnus (including K. e. ellipsiprymnus and K. e. defassa), K. kob, K. leche, and K. megaceros support the validity of these taxa, as well as the need to manage them as separate populations.
Investigation of renal cortical tissue in 5 adult hartebeests (Alcelaphus buselaphus cokii), 3 impalas (Aepyceros melampus), 1 defassa waterbuck (Kobus defassa) and 5 goats (Capra hircus) revealed large granulated peripolar cells at the junction between the parietal and the visceral epithelial layers of the renal corpuscles. All four animal species under study contained 1 or more peripolar cells for the majority of renal corpuscles sectioned through the vascular pole. In the hartebeests, up to 3 parietal cells and the first podocyte were granulated. Peripolar cells contained intracytoplasmic electron-dense membrane-bounded granules-200-2,800 nm in diameter in the hartebeests, 200-1,740 nm in the impalas, 150-950 nm in the waterbuck and 200-2,140 nm in the goats. Epithelioid granulated juxtaglomerular cells around afferent and efferent arterioles were rarely seen. When observed, they contained smaller granules than those of the peripolar cells. This distribution suggests that peripolar cells play a role in the regulation of body electrolytes and water, probably acting in concert with the renin-angiotensin-aldosterone system.
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In rams a positive correlation (P less than 0.001) existed between average testosterone levels from 30-min blood sampling for 18 h and average testosterone levels of samples taken 0, 1 and 2 h after injection of LH-RH administered 90 min after anaesthesia. Attempts were therefore made to assess testosterone status by LH-RH challenge and limited blood sampling in animals immobilized in their natural habitat. In impala (Aepyceros melampus) territorial males had higher plasma testosterone values than did bachelors after LH-RH challenge (8.1 compared with 2.6 ng/ml, P less than 0.05). In blesbok (Damaliscus dorcas), the relationship was less clear, but testicular volume was correlated with plasma testosterone concentration and with testicular responsiveness measured by testosterone produced per unit of LH (P less than 0.001 and P less than 0.05, respectively). The LH-RH challenge technique therefore has value as a measure of testicular function and permits study of ungulates in their natural environment.
A 10-mo-old female eland (Taurotragus oryx) at the Wilds exhibited recalcitrant, progressive unilateral uveitis for a 5-wk period, despite constant medical treatment. Unilateral enucleation was performed because of blindness and animal discomfort evidenced by continuous blepharospasm. Histopathologic examination of the eye demonstrated intraocular larvae morphologically consistent with Parelaphostrongylus tenuis, the first known case of intraocular P. tenuis migration. This animal subsequently was euthanatized because of severe, nonresponsive neurologic signs associated with P. tenuis infection.
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Longistrongylus curvispiculum was recovered from free ranging sable (Hippotragus niger), addax (Addax nasomaculatus) and biesa oryx (Oryx beisa) on two ranches in central Texas (USA). These nematodes represented a small portion of the total abomasal worm burden dominated by Haemonchus contortus. Female L. curvispiculum easily could be confused with other ostertaginae, but the spicules of the males were diagnostic.
The numbers and species of helminths recovered from one black wildebeest, Connochaetes gnou, three eland, Taurotragus oryx, 18 mountain reedbuck, Redunca fulvorufula, one red hartebeest, AIcelaphus buselaphus and two springbok, Antidorcas marsupialis, in the Mountain Zebra National Park, Eastern Cape Province; two black wildebeest, two grey rhebuck, Pelea capreolus, two mountain reedbuck and four springbok in the Karoo National Park, Western Cape Province; two bontebok, Damaliscus pygargus dorcas, two eland, two gemsbok, Oryx gazella and two springbok in the West Coast National Park, Western Cape Province; and a single springbok on a farm near Bredasdorp, Western Cape Province, are recorded. Nematodes belonging to a total of 12 genera and 20 species were identified. A single cestode was also recovered. Sixteen new host associations are recorded for the nematodes and one for the cestode Moniezia benedeni. Nematodirus spathiger had the widest host spectrum and with the exception of black wildebeest, was collected from all the host species examined.
We evaluated selection bias in a prospective study of 1,162 consecutive patients with suspected pulmonary embolism. Of these, 983 were eligible, and 627 could actually be included. During two months extensive data were collected on all non-included patients. Finally, our patient characteristics were compared with those of the PIOPED study (1990) and the study of Hull et al. (1994). Compared with included patients, the non-included patients had more often non-diagnostic V/Q scans (50% vs. 36%, p <0.01) and were more often already hospitalized (31% vs. 22%, P = 0.04). The subgroup of patients not included due to refusal or inability to give informed consent (IC) was older (mean age 61 vs. 53 years, P <0.01), more often suffered from malignancies (26% vs. 11%, P <0.01) and frequently had non-diagnostic V/Q scans (57%) as compared to included patients. In our study, 54% of all patients screened was eventually included versus 27% in the PIOPED study. In the PIOPED study patients who had contra-indications for pulmonary angiography were excluded, while in the study of Hull et al. those with inadequate cardiorespiratory reserve were excluded. In studies on new diagnostic technologies, patient selection bias does occur. The potential for such a selection bias should be taken into account when diagnostic strategies are devised to improve their generalizability and acceptability.
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