Concurrent bovine virus diarrhoea virus and Campylobacter fetus infection in an aborted bovine fetus.
Explore the source record for details and available documents.
Abortion, Veterinary↗
SEARCH · Search PubMed
Results for “Aborted Fetus”
Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Live fetuses in abortions pose problem for doctors.
Explore the source record for details and available documents.
Aborted Fetus↗
Genetic changes in human fetuses from spontaneous abortion after in vitro fertilization detected by comparative genomic hybridization.
The in vitro fertilization (IVF) technique is becoming a very important approach for infertile disease therapy, but approximately 30% of pregnancies are spontaneously aborted in the first trimester. It is believed that chromosomal abnormality is the major reason for early spontaneous abortion. Although some reports have mentioned cytogenetic changes in spontaneously aborted embryos after IVF, little is known about the comprehensive cytogenetic alterations in these aborted embryos. Here we use the comparative genomic hybridization (CGH) technique to analyze the genetic alterations in 41 spontaneously aborted human specimens after IVF. In this study, 25 of 41 cases (61%) showed chromosomal changes. Among them, autosomes and sex chromosomes were involved in 16 and 11 cases, respectively. Several nonrandom chromosomal changes were identified, including loss of one sex chromosome (six cases) and gains of 22 (four cases), Y (four cases), 21 (three cases), 4 (two cases), and 13 (two cases). Our data support the opinion that chromosome abnormality is one of the major causes of early spontaneous abortion after IVF. The association between chromosome changes in these spontaneously aborted fetuses and maternal age, infertility patterns, infertility causes, and IVF patterns (routine IVF and other methods, including intracytoplasmic sperm injection, egg donation, and embryo donation) were also studied. No significant correlation was found.
Abortion, Spontaneous↗
Cytogenetic abnormalities among spontaneously aborted previable fetuses.
We have reviewed spontaneously aborted fetuses of 9 to 20 developmental weeks. All fetuses with either external or internal developmental defects and those with maternal history of repeated spontaneous abortion were studied cytogenetically. Among 723 fetuses the cytogenetically proven prevalence of chromosome abnormalities was 5.8%. Inclusion of suspected chromosome abnormality based on morphologic findings resulted in an overall prevalence of only 7.1%. This prevalence and restricted spectrum of chromosome abnormalities, which included monosomy X, triploidy, gonosomal aneuploidy, trisomies 13, 18, and 21, resembled the prevalence and spectrum found among perinatal deaths and differed both in prevalence and type from those chromosome abnormalities found in spontaneous abortions occurring during the embryonic period. Monosomy X was the most common chromosome abnormality among previable fetuses. Both monosomy X and triploidy were more frequently detected in the early fetal period than in perinatal deaths. The combined prevalence of autosomal trisomies was equal to that which has been reported in the perinatal deaths. Chromosomal structural abnormalities, seen in perinatal deaths, were not found in our study.
Abortion, Spontaneous↗
Antibodies to Leptospira in the sera of aborted bovine fetuses.
Antibodies to Leptospira serotypes were detected in sera from 15 (6.9 per cent) of 218 aborted fetuses, but were not detected in the sera from 196 non-aborted fetuses. Fourteen (6.4 per cent) sera from the aborted fetuses had antibodies to strains belonging to the Hebdomadis serogroup and 1 (0.5 per cent) had antibodies to a strain belonging to the Icterohaemorrhagiae serogroup. These antibodies were predominantly immunoglobulin M whereas in the dams, antibodies belonged to both immunoglobulin M and G classes.
Abortion, Veterinary↗
Humoral immune response to infectious agents in aborted bovine fetuses in Argentina.
The purpose of this study was to characterize the exposure of bovine aborted fetuses from beef and dairy herds of the humid pampas of Argentina to different infectious agents by the evaluation of fetal fluid antibodies. Presence of fetal antibodies to bovine viral diarrhea virus genotype 1 (BVDV-1), bovine herpes virus type 1 (BHV-1), Leptospira interrogans, Brucella abortus, and Neospora caninum was determined. Of the 95 fetuses processed, 66 came from 49 beef herds and 29 from 12 dairy herds. The average gestational age of the aborted fetuses was 7.1 months. Antibodies to the mentioned agents were detected in 65 of the 95 fetal fluids (68.4%). In addition, antibodies to more than one infectious agent were detected in 32 fetuses (33.7%), suggesting fetal exposure to multiple antigens during gestation. There were antibodies to BVDV-1, BHV-1, N. caninum and Leptospira interrogans in 43 (45.2%), 29 (30.5%), 26 (27.4%) and 5 (5.2%) specimens, respectively. Antibodies to B. abortus were not detected in any of the fetal fluids. The results of this study provide information on the determination of antibodies in fluids from bovine aborted fetuses exposed to different infectious agents in the region.
Abortion, Veterinary↗
Using fetus after abortion.
Explore the source record for details and available documents.
Aborted Fetus↗
Isolation of Aino virus from an aborted bovine fetus.
A male fetus of gestation day 187 was aborted from a Holstein-Friesian cow in an epizootic of the Aino virus (AINOV) in September 1995. Neutralizing antibody titers against AINOV were 1:128, 1:16 and 1:64 in the dam serum, fetal ascites and cerebrospinal fluid, respectively. A 10% brain suspension of the aborted fetus was prepared immediately after autopsy, rinsed three times and sonicated before centrifugation. The supernatant was then inoculated into HmLu-1 cell cultures. A cytopathic effect was noted on post-inoculation day 7. The isolated virus was identified as the AINOV based on the physicochemical properties and cross neutralization test. This is the first report on the isolation of AINOV from an aborted bovine fetus.
Abortion, Veterinary↗
Serologic and molecular characterization of an abortigenic strain of equine arteritis virus isolated from infective frozen semen and an aborted equine fetus.
A virus isolated from an aborted equine fetus was determined to be antigenically distinct from several other strains of equine arteritis virus (EAV) by use of a neutralization assay with a large panel of neutralizing monoclonal antibodies. The virus was readily neutralized by polyclonal equine anti-EAV serum. Comparative nucleotide and amino acid sequence analyses indicated that the virus (WA97) isolated from the aborted fetus was virtually identical to a virus (S1971) isolated from imported semen used to inseminate another mare on the farm. Phylogenetic analysis indicated that the WA97/S1971 virus was more related to European than to North American strains of EAV. These sensitive molecular procedures may be useful for epidemiologic investigations of EAV infections. Screening and certification of stallions and frozen equine semen would prevent dissemination of pathogenic strains of EAV.
Abortion, Veterinary↗
Ethical and legal issues regarding selective abortion of fetuses with Down syndrome.
Selective abortion of fetuses with Down syndrome was discussed in terms of current abortion perspectives, genetic testing, legislation, and ethical principles. Legal doctrine and practice were presented in terms of the disparate treatment given the fetus with Down syndrome as compared to the fetus with no apparent disabilities. Finally, the ethical principles of autonomy, beneficence, nonmaleficence, fidelity, and justice were offered as guidelines for the examination of the legal double standards imposed by current legislation.
Abortion, Eugenic↗
Buenos Aires, a new Leptospira serovar of serogroup Djasiman, isolated from an aborted dog fetus in Argentina.
This study describes the isolation of a new leptospiral serovar from the Djasiman group from an Argentinean aborted fetus of a dog. The strain was isolated from a culture of mixed liver and kidney tissue from one aborted dog fetus. Bitch's serum showed a titre of 1:800 against the new serovar and 1:400 or less against other serovars tested. Microscopic Agglutination test (MAT) with a panel of 38 rabbit anti-Leptospira sera representative for all pathogenic serogroups was performed to establish the putative serogroup of the isolated strain. Serovar identification was done by Cross-Agglutination Absorption Test (CAAT). Species determination was carried out on basis of sequence identity of primer pair G1/G2 generated PCR products from the isolate with those from reference strains belonging to the various pathogenic Leptospira species. Results showed that the new strain named, Baires, belongs to species L. interrogans sensu stricto and the serogroup Djasiman. However, the isolate could not be identified as any of the known serovars within this serogroup. These results allow us to conclude that the strain Baires represents a new serovar (Buenos Aires) of Djasiman serogroup.
Aborted Fetus↗
Moral reflections on vaccines prepared from cells derived from aborted human fetuses.
Explore the source record for details and available documents.
Aborted Fetus↗
Concurrent conjunctivitis and placentitis in aborted bovine fetuses.
Consistent histopathological lesions were found in 10 out of 136 aborted fetuses examined during a three year period, using a multi-disciplinary diagnostic investigation technique. Fetuses exhibited a generalized mononuclear inflammatory cell infiltration, accompanied by distinctive lesions of conjunctival hyperplasia and goblet cell formation, alveolitis, and necrotic placentitis. In two cases where amnion was also examined, a chronic amnionitis was present. No consistent laboratory findings could be related to these cases. The fetal and placental lesions described were similar to those associated with experimental inoculation of Ureaplasma diversum in pregnant cows, and with field isolations of the same organism in aborting cattle.
Abortion, Veterinary↗
Isolation of Campylobacter jejuni from an aborted caprine fetus.
Campylobacter jejuni was isolated in pure culture from the tissues and stomach contents of an aborted fetus from a mixed-breed Illinois goat herd. In the herd, 5 of 21 does aborted in late gestation. The does had diarrhea before or concurrent with the abortions. Does that did not abort were vaccinated with ovine C fetus bacterin and were given chlortetracycline orally at the rate of 75 mg/day for 2 weeks. Further abortions did not occur.
Abortion, Veterinary↗
Cryptococcal pneumonia and abortion in an equine fetus.
Cryptococcus neoformans was the causative agent of pneumonia in a 9-month-old equine fetus aborted by a healthy American Paint mare. Endometritis was diagnosed on biopsy, and vaginal specimens obtained for culture were Cryptococcus-positive 1 month following abortion but not 5 months after abortion. Infection resolved without treatment between 1 and 5 months after abortion, and the mare was bred the following year and delivered a live premature foal without evidence of Cryptococcus infection.
Abortion, Veterinary↗
DNA hypomethylation of individual sequences in aborted cloned bovine fetuses.
Cloned bovines have a much higher abortion rate than those derived in vivo. Available evidence indicates that inappropriate epigenetic reprogramming of donor nuclei is the primary cause of cloning failure. To gain a better understanding of the DNA methylation changes associated with the high abortion rate of cloned bovines, we examined the DNA methylation status of a repeated sequence (satellite I) and the promoter regions of two single-copy genes (interleukin 3/cytokeratin) in aborted cloned fetuses, aborted fetuses derived from artificial insemination (AI), cloned adults and AI adults by bisulfite sequencing and restriction enzyme analysis. Two of four aborted cloned fetuses show very low methylation levels in the two single-copy gene promoter regions. One of the two fetuses also showed undermethylated status in the satellite I sequence. The other two aborted cloned fetuses have similar methylation levels to those of aborted AI fetuses. However, no difference in methylation was observed between cloned adults and AI adults. Our results demonstrate for the first time the undermethylated status of individual sequences in aborted cloned fetuses. These findings suggest that aberrant DNA methylation may contribute to the developmental failure of cloned bovine fetuses.
Aborted Fetus↗
Sarcocystosis in an aborted bovine fetus.
Sarcocystosis was diagnosed in an aborted bovine fetus. Immature and mature schizonts of Sarcocystis were disseminated in the vascular endothelium of all organs, but especially the brain. Microscopic granulomas, focal gliosis, and petechial hemorrhages in the neuropil were scattered in the brain. Multifocal collections of mononuclear cells were observed in the kidney, liver and heart. Organisms in sections of frozen tissues were demonstrated by immunofluorescent techniques to be Sarcocystis.
Abortion, Veterinary↗
Demonstration of equine herpesvirus-1 gene expression in the placental trophoblasts of naturally aborted equine fetuses.
Equine herpesvirus-1 (EHV-1) infection was demonstrated in the lung tissue of seven aborted fetuses by immunohistochemical labelling and polymerase chain reaction. The placentas of the fetuses were also examined by non-isotopic in-situ hybridization for the EHV-1 glycoprotein B (gB) gene. Positive hybridization signals were observed in the cytoplasm of trophoblasts, especially in microcotyledons, of all seven placentas, and in villous epithelium of the allantochorion of six placentas. Despite the presence of EHV-1 RNA, EHV-1 antigens were not detected in placentas by immunohistochemical examination. The present study represents the first in-vivo demonstration of the EHV-1 gene in equine trophoblasts. The findings suggest direct cell-to-cell spread of EHV-1 from endometrial cells to trophoblasts.
Abortion, Veterinary↗