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At least 1,423 records · Page 79Linked to original sources
PubMed2003

Expression level of Ubc9 protein in rat tissues.

Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDa band of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein appears to have some degree of tissue specificity.

Animals↗
PubMedDate not supplied

Expression of the epidermal growth factor receptor in squamous carcinoma cell lines of the larynx and hypopharynx.

Characterization of the EGF-R has been carried out on five squamous carcinoma cell lines of the head and neck region (FaDu, HLaC-78, HLaC-79, UM-SCC-17A and 17-B). The results have been compared with those from a spontaneously immortalized aneuploid human keratinocyte cell line (HaCaT) and cultured fresh skin keratinocytes. A monoclonal antibody directed against the external region of the EGF-R was used for APAAP-immunostaining and flow cytometric analysis. We observed an important heterogeneity in the EGF-R expression of the different cell lines. The highest overexpression of the EGF-R was found on the cell line UM-SSC-17A. The results of our flow cytometric studies confirm and extend previous studies in which either radioimmunoassay of tissue extracts or immunocytochemistry of tissue sections showed an altered expression in head and neck squamous carcinoma. The cell line UM-SSC-17A seems to be most useful in elucidating the role of the EGF-R expression in the behaviour of head and neck epidermoid malignancies.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

TPS, thymidine kinase, VEGF and endostatin in cytosol of thyroid tissue samples.

The aim of the study was to determine whether VEGF, TPS, TK or Endostatin determination in tissue cytosol may have some additional value in distinguishing among different types of thyroid lesions. These markers were chosen as representatives of the 2 main pathways (angiogenesis and proliferation) involved in thyroid diseases. VEGF is the most potent angiogenic promoter and Endostatin plays an opposing role. Thymidine kinase (TK) is a marker of DNA synthesis and TPS, cytokeratin 18 fragments, is a marker of the rate of proliferation. We determined qualitatively all four markers in tissue extracts: cytosol from 157 tissue specimens (93 goitre, 12 Hashimoto's thyroiditis, 39 adenomas and 13 carcinomas). In 6 cases we were able to compare both normal and pathological tissue samples from a single patient. Statistically significant differences were found in the measured markers, but outliers were present in all groups. This fact does not permit their use in differential diagnosis. The highest levels of all markers were reached in adenomas, being higher than in carcinomas, probably explained by the higher overall metabolic rate in adenomas.

Cytosol↗
PubMed1989

Serum and gingival tissue antibody levels to oral microbial antigens in human chronic adult periodontitis.

Antibody levels against antigens from eight oral micro-organisms were measured in sera and gingival tissue extracts from adult periodontitis patients and healthy subjects by an enzyme-linked immunosorbent assay. The antibody levels of diseased serum samples were significantly higher than those of healthy serum samples. Analysis of diseased sera indicated that antibody levels against Bacteriodes gingivalis antigens were significantly higher than antibody levels against all the other antigens tested except for those from B. intermedius and B. asaccharolyticus. Extracts from diseased gingival tissues had significantly higher levels of antibody against B. gingivalis antigens than against antigens from all of the other micro-organisms tested, including B. intermedius and B. asaccharolyticus. These results suggest selective, specific and localized antibody production during or following the establishment of chronic adult periodontitis.

Adult↗