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At least 1,423 records · Page 79Linked to original sources
PubMed1992

Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters.

The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride-methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifluoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90 degrees C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.

Animals↗
PubMed1982

Metabolism of 1-0-[1'-14C]octadecyl-sn-glycerol in Leishmania donovani promastigotes. Ether lipid synthesis and degradation of the ether bond.

A long-chain 0-alkylglycerol, 1-0-[1'-14C]octadecyl-sn-glycerol, was taken up and metabolized extensively in Leishmania donovani promastigotes grown on a lipid-free, semi-defined medium as well as on a lipid-free, synthetic medium in nearly identical ways. Cleavage of the ether bond was the main event even after short incubation times (1 h) and low precursor concentration (2 microM), as judged from the appearance of radioactive acyl moieties residing mainly in phosphatidylcholine. In addition, labeled plasmanyl inositol and plasmenyl ethanolamine were formed to a considerable extent. However, when administered in higher concentrations (25 microM), 1-0-alkyl-sn-glycerols as well as their enantiomers inhibited lipid metabolism dramatically, accompanied by rounding of the cells and appearance of cellular debris in the medium. This effect was not dependent on the aliphatic side chain (12:0, 16:0, 18:0, 18:1) of the 0-alkylglycerols. It is suggested that, by incorporation into cellular membranes, 0-alklglycerols affect factors responsible for the interaction of membranes and the cytoskeleton as well as enzymes of lipid metabolism.

Animals↗