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At least 1,423 records · Page 79Linked to original sources
PubMed2005

Numerous erythroblasts in maternal blood are impervious to fluorescent in situ hybridization analysis, a feature related to a dense compact nucleus with apoptotic character.

BACKGROUND AND OBJECTIVES: The analysis by fluorescence in situ hybridization (FISH) of fetal erythroblasts enriched from maternal blood remains an attractive alternative for risk-free prenatal diagnosis of aneuploidies. However, current results are discouraging because of the low levels of sensitivity or the inability to detect fetal erythroblasts by FISH. DESIGN AND METHODS: Erythroblasts were enriched from 35 maternal blood samples by magnetic cell sorting (MACS), identified morphologically following May-Grünwald Giemsa staining and examined by FISH for chromosomes X, Y and 18. RESULTS: We observed that circulating erythroblasts comprised two distinct groups: one was clearly of maternal origin and could be reliably analyzed by FISH, whereas the other, which appeared to be of fetal origin, was largely impervious to FISH analysis. This latter feature seemed to be related to an abnormally dense nucleus with an apoptotic character. Since the oxygen tension in the maternal circulation is higher than that in the fetus, we cultured fetal cord blood erythroblasts in conditions mimicking this difference in oxygen concentrations and found that high oxygen concentrations rapidly induced shrinkage of the erythroblast nucleus, rendering it impervious to FISH analysis. INTERPRETATION AND CONCLUSIONS: Our data show that circulating erythroblasts of presumed fetal origin cannot be reliably analyzed by FISH because of an abnormally dense nucleus. This nuclear phenotype appears to be induced by the higher oxygen tension present in the maternal circulation than in fetal blood.

Aneuploidy↗
PubMed2005

[Pridiction of the Sex-effects of the Cocoon Quality Characters in Silkworm.].

In this paper , the sex-effects of the cocoon quality characters in silkworm was predicted with Mixed linear model úThe fact that the probability of effect variance and predictability of random gender of whole cocoon weight, cocoon shell weight, ratio of cocoon shell and pupa weight reached a level of extreme significance showed that the gender effect of the four traits was extremely significant, which matched with the reality completely. The predictive values of gender effect of the four traits of female(male) were 0.248g (-0.247g), 2.423cg(-2.394)cg, -1.976%(1.992%) and 0.224g(-0.223g) respectively. Each trait showed single peak distribution after adjusting by sex-effects, which fitted for the request that quantitative traits should show continuously normal standard distribution if QTL analysis was taken.

Animals↗
PubMed2005

[Isolation of PVA-degrading symbiotic bacteria and examination of degradation characters].

From printing and dyeing actived sludge, PVA- degrading symbiotic bacteria was isolated. The symbiotic bacteria is composed of B1 and B2. During PVA biodegradation, B1 produces a certain growth factor for B2, B2 produces PVA-degrading enzyme. The PVA-degrading characters of the symbiotic bacteria are examined. When the concentration of PVA was 0.1, 0.5, 1g/L respectively,more than 80% of PVA was degradated after 7days. During the treatment of the dyeing and printing wastewater, when the temperature was 30 degree C ,the removal of COD(Cr) was 69.75%, the proper treating time was 12h.

Bacteria↗
PubMed2005

[Early characters of recurred vocal cord cancers after laser cordectomy and treatment].

OBJECTIVE: To explore the characters of recurred vocal cord cancer in early phase after laser cordectomy and selection of the second treatment. METHOD: Five cases who had recurrence after laser cordectomy was selected and analyzed according to the scope of the tumor, operation type, recurrence time, site of recurrence. RESULT: One case's had the first recurrence two months after the first operation and was treated by laser once more, and the second recurrence happened three months after the second operation and no recurrence was found within the 4 years after the third operation-extended vertical hemilaryngectomy. Two cases had recurrence 3 months after the first operation and one case 6 months later. The three cases had extended vertical hemilaryngectomy for remedy and had no recurrence within 4 years. The last one had recurrence 1 year after operation, and received a total laryngectomy. The site of recurrence was the anterior commission in 4 cases and median part of vocal cord in one case. The 5 cases included 3 T1a and 2 T1b. The recurrence rate of T1a and T1b were 3.1% and 50.0% respectively. CONCLUSION: The recurrence time after laser cordectomy is often within half a year. The most common site of recurrence is anterior commission. Extended partial laryngectomy should be the first choice remedy. Strict selection of indication and skillful technique are the base of good efficacy.

Adult↗
PubMed2005

[Dynamic characters of sulphur and heavy metals concentrations in Pinus taiwanensis growth rings].

Based on the concentration analyses of S, Mn, Fe, Zn, Cu and Pb in the growth ring set of Pinus taiwanensis at the summit (1,400-1,600 m alt.) of Huangshan Mountains during past 80 years, this paper studied the dynamic characters of element concentrations in the ring set, and their relationships with atmospheric environment. The results showed that there were three levels of S concentration in the growth ring set, i.e., low concentration (less than 2 mg.kg(-1)), accounted for 58.5% of the ring set; medium (3.65-6.0 mg.kg(-1)), accounted for 24.4%; and high ( > 11.0 mg.kg(-1)), accounted for 17.1%. The dynamic change of S accumulation in the ring set displayed an obvious fluctuation, which could be divided into 3 major types, i.e., fluctuation during 1917-1960, relatively stable during 1961-1980, and gradual increase after 1981. In the growth ring set of 1935-1938, 1959-1960, and recent 10 years, the S accumulation concentrations were all higher than 11 mg.kg(-1), suggesting the relatively high atmospheric S concentration in those periods. There was a significant correlation between the increase of tourist amount in Huangshan scenic spot and the S concentration in growth ring set. The fuel fume centralized in some places might result in the atmospheric pollution at local scale, which in turn, might influence the S accumulation in growth ring set. The Pb accumulation concentration in the ring set was less than the background value in soil. The accumulation concentrations of Mn, Fe, Zn and Cu showed fluctuation characteristics, with independence of each other. The accumulation of Zn and Cu in growth rings had no correlation with tree age, while that of Mn and Fe was in adverse. The Mn concentration in the growth ring set decreased with tree age but increased in recent growth rings, while the Fe concentration was on the contrary, which needed further study.

Metals, Heavy↗
PubMed2002

[Gene frequency of 12 characters in 5 nationalities in inner mongolia].

The gene frequency of 12 characters was reported in Ewenki, Oroqen, Daur, Mongol and Han nationalities in Inner Mongolia, and compared among these nationalities. The result indicated that the difference of Mongoloid fold among nationalities was significant, followed by the Folding tongue, while the difference of Handedness and Nasal profile was relatively insignificant.

English Abstract↗
PubMed2005

[Distribution and characters of heavy metals from municipal solid waste incinerator fly ash].

The distribution and characters of heavy metals from municipal solid waste incinerator fly ash was studied in East China. The result indicate that heavy metals in fly ash were mainly distributed in fine particles. The heavy metals including Cd, Pb, Mg, and Cu could migrate easily from fly ash while Ag and Sn would be more stable. The metal mobility changed with the pH level. For environmentally important metals such as Cd, Co, Cu, Mn, Ni, Pb, and Zn, the increase in mobility due to the decrease in pH was more pronounced and significant than for other elements, while Cr and As with the opposite condition.

Air Pollutants↗
PubMedDate not supplied

Association analysis of some drought related characters in hexaploid spring wheat (Triticum aestivum L.).

The association among yield components and their direct and indirect influence on the grain yield of wheat were investigated. 24 breeding lines were tested in a randomized complete block experiment design with three replications. According to the results the phenotypic correlation among the traits and their path coefficient were estimated. Positive significant correlation coefficients were obtained for association between survival rate treatment I, III, leaf venation, stomatal frequency, osmotic pressure, flag leaf area and number of tillers per plant with grain yield per plant at both phenotypic and genotypic levels. Negatively significant correlation between hygrophilic colloids and epidermal cell size with grain yield per plant was obtained at phenotypic and genotypic levels. Path coefficient was also computed to estimate the contribution of character to the yield. Path coefficient analysis revealed that flag leaf area, root/shoot ratio and survival rate II had the highest positive direct effects on grain yield, while hygrophilic colloids and osmotic pressure had negative direct effect on grain yield. These results thus obtained suggested that flag leaf area is an important component of yield and hence needs special attention in selection strategies.

Biomass↗
PubMed2005

[Culture, purification and biological characters of olfactory ensheathing cells from adult transgenic mice].

Several studies have demonstrated the potential of olfactory ensheathing cells (OECs) for the repair of central and peripheral nerve injury. In this work, ensheathing cells that express the receptor gene coding for enhanced green fluorescent protein (eGFP) from adult mice were isolated and purified, and then, its biological characters were examined in vitro. The work was based on combinations of fluorescence confocal, phase contrast, cell proliferation assay and immunolabeling identification. The results showed that (1) two major morphologically and immunohistochemically distinct types of cells were present after 15 days in the primary cultures of adult transgenic mice olfactory nerves and glomerular layers of the olfactory bulb. One cell type was bipolar or multipolar OECs and strained positively for P75 low affinity neurotrophic receptor (P75N), S100, and glial fibrillary acidic protein(GFAP). The other type was fibroblasts with flat or endothelial-like shape, and reacted with antibody against Thy1.1. (2) A simple, inexpensive method for purifying ensheathing cells, in which various harvested cell types showed different rates of attachment to the uncoated culture ware, was developed. This technique neither binds any antibodies nor requires any costly equipment, and yields a large number of highly purified cells. (3) In sequential observations over 22 days in culture, the population of purified cells was maintained and continued to proliferate for longer. This experiment not only supported and advanced the ensheathing cell research but also offered ideal materials of in vivo transplantation for the repair of CNS injury.

Animals↗
PubMedDate not supplied

Biological characters and bacteriophage typing of Staphylococcus aureus isolated from chicken staphylococcosis and commercial balanced chicken food in Argentine.

During 1976-78, 33 S. aureus strains were isolated from chickens suffering from dermatitis and septicemia, omphalitis, pneumonia, arthritis and tenosynovitis; and from a commercial balanced chicken food. All the strains were classified into the biotype B of Hájek and Marsálek. Twenty-nine (87.8%) strains could be classified into Sato et al biological type 4 although they differed from it in its ability to grow at 45 degrees C. The remaining 4 strains differed from the others because they failed in producing hemolysis in bovine and ovine blood agar, egg yolk reaction and splitting of Tween 80. Thirty (90.9%) strains were lysed by avian phages: 28 strains at 1 x RTD and 2 at 100 x RTD. Twenty seven (93.1% strains of the Sato et al biotype 4 were sensitive to the phages CH42/CH43/CH63/CH37 of avian group II, and 2 strains to the phage CHA1 at 100 x RTD. Only one of the 4 unclassified strains could be typed by phage CH4 of avian group I at 1 x RTD. Twenty seven (81,8%) strains were typable at 100 x RTD by the basic set of human phages: 26 strains belonged to group III (53 and 53/77) and 1 to a mixed group I/III (79/53/77). The typable strains belonged only to Sato et al biotype 4. None of 33 strains were sensitive to any of the bovine phages. The biological characters and bacteriophage typing of the S. aureus showed that almost all the strains belong to chicken types. The strains isolated from food belong to the same chicken biotype and phagetype as the strains isolated from dermatitis, septicemia, omphalitis and pneumonia. The strains isolated from arthritis and tenosynovitis were different from the ones isolated from other pathologies.

Animal Feed↗
PubMed2005

[The prevalence and character of primary headache in Japanese high school students].

We investigated prevalence and character of primary headache for 2,462 examples of high school student. An overall headache prevalence was 41.0 in boys, 55.3% in girls, and average of the headache onset was 12.7 in boys, 12.9 in girls years old. A prevalence of migraine is 5.5 in boys, 6.1% in girls according to ICHD-I and 13.7 in boys, 17.5% in girls according to modified ICHD-II diagnosis criteria. For the tension type headache, it was 23.0 in boys, 30.6% in girls according to modified ICHD-I diagnosis criteria. High school students with primary headache tend take painkillers easily, but are much less likely visit physicians (only 15.0%). Our data showed that the prevalence of headache especially migraine in Japanese high school student was almost similar to past world wide findings, and suggest that its impact on the quality of life can not be ignored. Therefore, headache education must be important and necessary in a high school.

Adolescent↗
PubMed2005

[Splicing site mutation of D19S418 in PRPF-31 gene and its phenotypic characters with autosomal dominant retinitis pigmentosa].

OBJECTIVE: To evaluate the disease-causing gene and phenotypic characters of a large family with autosomal dominant retinitis pigmentosa (adRP). METHODS: Disease status and associated ocular abnormalities of eight patients and six unaffected members who represent different generations of this family were assessed by measurement of visual psychophysics, full-field and multifocal electrophysiology (ERG and mfERG) and funds fluorescent angiography (FFA). The DNA samples of nineteen patients and fifteen unaffected individuals in this family were examined by Genome scanning, linkage analysis and mutation detection to identify coding sequence changes. RESULTS: A case with variable, early onset night blindness before 10 years and visual field loss in their teens was found. Macular dystrophy, progressing to a retinitis pigmentosa phenotype was demonstrated in most adult cases. Both a-wave and b-wave amplitudes of photopic and scotopic full-field ERG were marked reduced and nearly non-detectable, demonstrating severe damage of photoreceptor systems. There were two obligate gene carriers in the family which remained asymptomatic in the clinical. But one of them was found with a minimal RP characteristic and the other was normal by examination of fundus and ERG. An unreported splicing site mutation (IVS5-1G > A) was identified in intron 5- acceptor site of PRPF-31 gene on chromosome 19. ERG and molecular genetic findings were consistent with the reclassification of this disease as an autosomal dominant RP. CONCLUSION: It is a novel splicing site mutation that IVS5-1G > A of D19S418 site in PRFP31, the relative phenotypes by which main displayed type I/diffuse has variable expressivity and complex phenotype.

Adolescent↗
PubMed2005

[Clinical, pathological and radiological characters of appendiceal mucocele].

OBJECTIVE: To improve capability of appendiceal mucocele diagnosis via analyzes its clinical, pathological basis and radiological results. METHODS: Summarize 18 cases with pathological diagnosed appendiceal mucocele in PUMC Hospital since January 1985 till september 2004, including 4 males and 14 females (1:3.5), age ranged from 31 to 78 yrs old (average 59.8 years old). Eleven cases underwent barium enema, in which 9 cases received abdominal CT scanning and 1 case received CT virtual colonography. RESULTS: (1) CLINICAL RESULTS: 8 cases (44.4%) had right lower abdominal pain, 12 cases (66.7%) had palpable abdominal mass. (2) Radiological results: barium enema showed extrinsic oppressed filling defect at the end of cecum without destruction of mucosa; CT scanning showed right lower abdominal cystic mass, 75% with cystic wall calcification, massive septal mucous ascites was seen in one case with pseudomyxoma peritonei; CT virtual colonography showed clearly the extra cavity and intracavity changes at cecum. (3) Pathological results: simple mucocele (4 cases, 22.2%), mucous cystadenoma (12 cases, 66.7%) and mucous cystadenocarcinoma (2 cases, 11.1%) CONCLUSION: There are four pathological types in appendiceal mucocele. Radiological characters of appendiceal mucocele have distinct specificities while its clinical ones not. Radiological procedures are vital for correct clinical diagnosis.

Adult↗
PubMed2005

[Fine-root character and its action mechanism of forest at its initial reestablished stage on degraded red soil].

This paper studied the fine-root character and its action mechanism of forest reestablished by different models for 10 years on a seriously degraded red soil, and analyzed their correlation with soil property. The results showed that fine-roots mostly distributed in 0-20 cm soil layer, accounted for 73.39% -87.41% of those in 0-40 cm soil layer. There were notable differences in nutrient storage of fine-roots under different reestablishment models. The fine- roots of medium density pure Pinus serotina forest had a much higher storage of total nitrogen, phosphorus and potassium, with the content of total nitrogen, phosphorus, potassium, calcium and magnesium being 84.47, 5.55, 38.42, 17.00 and 10.76 kg hm(-2), respectively. Soil organic matter, total nitrogen, available nitrogen, available potassium and pH had significant correlations with fine-root biomass (P < 0.05). The effects of fine-roots on soil physical properties mainly reflected on soil capillary porosity and total porosity. Fine-roots obviously increased the contents of > 0.25 mm and > 5 mm soil waterstable aggregates, and improved soil structure-stability. The correlations between fine-roots and soil bacteria and microbial quantities were significant.

Carbon↗
PubMed2005

[Study on the original plants and macroscopic characters of Radix Paeoniae].

OBJECTIVE: For the identification of botanical origin and macroscopic characters of Radix Paeoniae. METHODS: Different types of original plant and crude of Radix Paeoniae samples were collected from 10 main producing areas. RESULTS: By taxonomical and morphological researches, Paeonia lactiflora and P. lactiflora var. trichocarpa could be identified. CONCLUSION: The root of P. lactiflora is cylindrical, pale white to reddish brown in surface color, with longitudinal furrows, flat borken section, with radial rows in xylem elements, sour taste. While the root of the variety can be differ from its irregular-cylindrical shape, brownish surface, floury transverse section and sweet taste.

Flowers↗
PubMed2006

Phenotypic characters of urinary isolates of Pseudomonas aeruginosa & their association with mouse renal colonization.

BACKGROUND & OBJECTIVE: Very few studies regarding production of virulence factors in different predominant serotypes of uropathogenic Pseudomonas aeruginosa are available and they have not been correlated to in vivo pathogenicity in the urinary tract. This study was carriedout with the objective to analyze the phenotypic characters of uroisolates of P. aeruginosa in vitro and to study the association of these virulence traits with their ability to cause nephropathogenicity in mouse model of ascending urinary tract infection (UTI). METHODS: Protease, elastase, alginate, haemolysin, pyochelin, pyoverdin and phopholipase C were measured using standard protocols in 18 uroisolates of P. aeruginosa isolated from patients suffering from complicated UTIs. An ascending model of pyelonephritis was established in Swiss Webster (LACA) female mice with these isolates. Quantitative bacterial count and histopathological evaluation of mouse renal tissue was done which were then assessed for a possible association with elaboration of virulence factors. RESULTS: All isolates of P. aeruginosa were able to colonize renal tissue of mice. However, renal counts varied amongst different isolates producing different virulence factors. Isolates producing high levels of haemolysin along with other virulence factors were able to colonize and multiply more in mouse renal tissue as compared to those producing low levels of haemolysin. INTERPRETATION & CONCLUSION: The findings of this study indicated an association between haemolysin production and renal colonization. High level of haemolysin production in vitro could be used as surrogate information for assessing pyelonephritic potential of P. aeruginosa.

Animals↗
PubMed2006

[The pathological characters and its clinical significance of internal hemorrhoids].

OBJECTIVE: To investigate the pathological characters and the corresponding clinical significance of internal hemorrhoids tissues. METHODS: Normal anal cushion and internal haemorrhoids tissue samples were obtained after stapled haemorrhoidectomy procedure from 24 grade III hemorrhoidal patients. The macroscopically normal cushions served as own controls and the normal cushions from a patient without a history of haemorrhoids as quality control. Routine Hematoxylin-Eosin and orcein were performed for elastic fibers. RESULTS: Compared with the corresponding normal anal cushions, the subepithelial vessels especially the cavernous vessels of the hemorrhoidal tissues showed obvious structural impair, retrograde changes, and the internal elastic lamina were ruptured and discontinuous. In addition, thrombosis and subsequent ischemic changes were observed. The Trietz's muscle and the fibro-elastic tissues showed hypertrophy, distortion, rupture and tortility. Obvious mucosal injury was observed in the mucous of hemorrhoidal tissues. Venous dilatation was infrequent in the hemorrhoidal tissues. CONCLUSIONS: The anal cushions of hemorrhoids disease patients show significant pathological changes. The pathological changes include structural impair, retrograde changes of the cavernous vessels and the hypertrophy, distortion, rupture and tortility of the Trietz's muscle and the fibroelastic tissues, and mucosal injury of the mucous membranes. These pathological changes are the basis of pathogenesis and development of hemorrhoids.

Adult↗
PubMed2006

[Construction of Tet-on inducible CXCR1 eukaryotic expression plasmid and identification of the expression character in NIH3T3 cells].

AIM: To construct tetracycline (Tet)-controlled inducible vector CXCR1-pTREhyg, and then detect the expression character of CXCR1 under the regulation of Dox in NIH3T3 cells. METHODS: A full length cDNA of human CXCR1 was cloned from sample of fibroblast like synovium (FLS) in rheumatoid arthritis (RA) patient by RT-PCR and then sub-cloned into the pTREhyg plasmid after sequence analysis. CXCR1-pTREhyg and pTet-on was co-transfected with Lipofect2000 to NIH3T3 cells, and the expression of IL-8RA was detected by Western blot after given different concentration of Dox. RESULTS: Western blot showed that CXCR1 could be induced by Dox in NIH3T3 cells, and the phosphorylated Erk-1/2 level was significantly increased after IL-8 stimulation. CONCLUSION: Tet inducible recombinant vector of CXCR1-pTREhyg was successfully constructed, and it could be expressed in NIH3T3 cells. The stimulation of IL-8 obviously changed the activity of Erk-1/2 in the transfected NIH3T3 cells. This work has laid foundations for further study on the relationship between CXCR1 and RA disease.

Animals↗