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Evaluation of a diagnostic protocol using screening diagnostic peritoneal lavage with selective use of abdominal computed tomography in blunt abdominal trauma.

BACKGROUND: The optimal method of evaluating blunt abdominal trauma remains controversial. A combination of a sensitive screening test, diagnostic peritoneal lavage (DPL), and a specific test, abdominal computed tomography (CT), may be a safe, efficient approach to adult blunt abdominal trauma. METHODS: A prospective cohort study compared a protocol using screening DPL followed by selective use of abdominal CT (DPL/abdominal CT) and the use of abdominal CT alone in the evaluation of hemodynamically stable, adult blunt trauma patients. RESULTS: One hundred sixty-seven adult blunt trauma patients were initially evaluated by DPL (n = 71) or abdominal CT (n = 96). Emergency department evaluation required less time in the DPL/abdominal CT group than in the abdominal CT alone group (41 minutes vs. 2.5 hours; p < 0.001). There were no missed injuries in the DPL/abdominal CT group versus seven missed injuries in the abdominal CT group (p = 0.02). There were no nontherapeutic celiotomies in either study group. CONCLUSION: Screening DPL, followed by abdominal CT if positive, is a safe, efficient method of evaluating adult blunt abdominal trauma that reduces the time required to evaluate the abdomen, does not result in increased nontherapeutic celiotomies, results in fewer missed injuries, and reduces the overall use of abdominal CT.

Abdominal Injuries↗

Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay.

A recently described PCR test for the identification of Neospora caninum and Toxoplasma gondii has been further developed and optimized in view of its practicability for routine diagnostic application. The N. caninum-specific PCR was adapted to the diagnostic operating standard of the T. gondii-specific PCR in that the uracil DNA glycosidase system was introduced, which eliminates potential carry-over contaminations of amplified target DNA from previous reactions. Furthermore, both PCR tests were optimized by including a DNA hybridization immunoassay based on the use of the commercially available Gen-eti-k DEIA kit. This assay allowed highly sensitive and specific detection of respective DNA amplification products and thus substantially facilitated the reading and interpretation of the test results.

Animals↗

Engineering CRISPR for Point-of-Care Tests.

CRISPR-based molecular diagnostics have emerged as powerful and programmable platforms that enable sensitive and specific detection for disease management and epidemiological surveillance. Advances in CRISPR engineering and assay design are driving the emergence of next-generation detection platforms that are highly sensitive, rapid, and amenable to field deployment. These engineering breakthroughs have the potential to reshape point-of-care tests (POCT) and transform how emerging and persistent health threats are monitored in decentralized and resource-limited settings. Herein, we systematically review the recent advancements in CRISPR engineering strategies aimed at improving detection sensitivity and specificity, eliminating the dependence on preamplification, and enabling robust POC deployment. The discussed strategies encompass both the rational engineering of CRISPR ribonucleoproteins (RNPs) and the optimization of downstream signaling modules for molecular diagnostic applications. We further highlight key challenges and future perspectives that may inspire impactful research directions and accelerate the advancement of CRISPR engineering strategies toward robust, field-deployable POCT platforms.

CRISPR-Cas Systems↗

Bimodality analysis of frequency distributions of 2-hour plasma glucose concentrations in the urban Micronesian population of Kiribati.

Bimodality in the frequency distribution of plasma glucose 2 h after a 75-g oral glucose challenge has been demonstrated in only a few population groups, and the generalizability of the phenomenon remains unclear. Therefore, we have studied the distribution of 2-h glucose in 1813 Micronesians, aged 20 years and over, from an urbanized community of the Pacific Ocean Republic of Kiribati. The 2-h plasma glucose distributions were consistent with a mixture model comprising two lognormal components for age groups 30-39, 40-49 and 50+ years. The parameters of the mixture distribution were estimated by the method of maximum likelihood. The prevalence of non-insulin-dependent diabetes mellitus was 7.3% as defined by World Health Organization diagnostic criteria, and 4.7% when defined according to age-specific optimal cut-off values based on the minimization of the total number of misclassified individuals. Assuming that the fitted mixture distribution for each age group was the true plasma glucose distribution, the sensitivity and specificity of the World Health Organization diagnostic criteria and the age-specific optimal cut-off values were determined. Higher sensitivity with relatively little impairment of specificity resulted with the use of the former criteria. The prevalence of non-insulin-dependent diabetes mellitus increased with age as did the mean and standard deviation of the lower lognormal glucose component ("nondiabetics"). However, the mean and standard deviation of the "diabetic' upper component remained relatively constant and varied little with age.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

[Unique children -- unique headaches. Case reports of pediatric headache patients from an outpatient children's pain department].

In the industrialized nations headache prevalence is increasing in children and adolescents. The nosologic classification determines the therapeutic strategy to follow. Three case reports illustrate the optimal cooperation of both a pediatric outpatient pain clinic and a pediatric psychosomatic pain clinic. We report on (1) a girl aged 2 years and 7 months with a 4-month history of headache episodes lasting about 15 min each with concomitant symptoms; (2) an 11-year-old boy with Schimmelpenning-Feuerstein-Mims syndrome, symptomatic focal epilepsy, psychomotor retardation, mild postinfectious internal hydrocephalus, and repeated heat-triggered episodes of right-sided headache beginning suddenly with a duration of 5-30 min and concomitant flush of his hemiface; and (3) a 12-year-old boy who for about 2 years has suffered from "migraine" 3 times a week, significantly impairing his quality of life. We discuss the patients' courses, diagnostic pitfalls, and therapeutic options. For the optimal treatment of children with headache not easily fitting into one of the categories, with significant comorbidity present, or if there is no adequate response to therapy conforming with guidelines, the help of an interdisciplinary pediatric pain clinic is invaluable.

Amines↗

[Transcranial dopplerography in the optimization of the treatment procedure in cerebral arteriovenous malformations taking into account the functional cerebral hemodynamics].

The incidence of vascular abnormalities in the pattern of central nervous diseases is steadily increasing. Arterioventricular malformations (AVM) are of great significance among cerebrovascular diseases to be surgically treated. A comprehensive examination of 57 patients with AVM, which involved cerebral angiography, transcranial Doppler at the stages of surgical treatment led to the conclusion that the application of AVM morphological characteristics alone did not allow the development of specific complications, hyperemic ones in particular, to be accurately predicted in the early postoperative period after elimination of malformation. It was suggested that due to the naturally significant relationship of Doppler indices of blood flow in the nutrient arteries having structural malformations and showing cerebral circulatory disorders, the use of values of the linear velocity of blood flow in and the reactivity and resistance in the nutrient arteries enables one to predict a risk for hyperemic complications after AVM elimination to a high precision and to define a preferable surgical intervention. The priority employment of transcranial Doppler in the diagnostic examination of patients with AVM will optimize an instrumental preliminary study, make a correct treatment policy, improve the outcomes of surgical intervention, and reduce the length of hospital stay.

Adolescent↗

Evaluation of diagnostic criteria for borderline personality disorder.

A comparison of 17 narrowly defined borderline patients with 20 nonpatient control subjects indicated that certain individual and combinations of criteria may be more highly correlated with the disorder than others. Requiring any four or certain specific combinations of two or three of the five most discriminating criteria provided the optimal balance of sensitivity, specificity, predictive power, and diagnostic efficiency considerations. Fewer than five DSM-III-R criteria adequately identified the patients.

Adolescent↗

The use of bitewing radiographs in the management of dental caries: scientific and practical considerations.

The objective of this paper is to synthesize published research on the use of radiographs in caries diagnosis in order to produce recommendations for guidelines that are clinically oriented but scientifically based. The options available include the use of various types of radiographic examination alone or in conjunction with other diagnostic aids. The outcomes should facilitate the optimal management of lesions as either preventive care advised (PCA) or operative care advised (OCA). Small initial lesions (PCA) require prompt detection, the application of appropriate preventive care and subsequent monitoring to maintain the most favourable tooth state achievable in the long term. Larger dentinal lesions (OCA) also require prompt detection so that appropriate high-quality operative care can be provided before further loss of tooth substance. Evidence was collected from the literature by updating several recent reviews by the author. The values employed were broadly analogous to those of the Canadian Task Force on the Periodic Health Examination. The use of ionizing radiation is always associated with a degree of risk: therefore all exposures must be kept as low as is reasonably achievable. Present evidence on the balance of risk and benefit indicates that the diagnostic yield for caries diagnosis is high enough to justify individualized examinations, particularly as changes in the morphology of caries have rendered clinical diagnosis of dentinal lesions less sensitive. This issue must be kept under review as alternative diagnostic technologies develop. There is good evidence that initial posterior bitewing radiographs are required for all new dentate patients over five years of age with posterior teeth. This procedure is required as an adjunct to clinical examination for the detection of caries on both the approximal and occlusal surfaces of the teeth. Although a 'blanket' regimen of routine radiographic examination at fixed intervals cannot be advocated, individualized bitewing examinations at varying frequencies determined on the basis of caries risk are supported. At the initial visit, an assessment of caries risk of the individual patient should be made. Varying intervals of first radiographic recall can then be suggested on the basis of differing degrees of risk. At present, risk assessment is imprecise, and risk status may change over time. Therefore, intervals between subsequent radiographic examinations must be re-assessed for each period. The purpose of detecting individual lesions should be to facilitate the planning of appropriate preventive treatment decisions based on lesion severity, caries risk and the patient. Different treatment should be employed for lesions in the PCA and OCA categories. Further rigorous studies are required to evaluate diagnostic methods appropriate for use in individual patient care, epidemiology and clinical research, and to increase the understanding of how findings from these applications inter-relate. Development and validation of reliable methods of caries risk assessment which are usable in general practice is a priority. Investigations of the processes involved in, and the outcomes of, dental decision-making are required to ensure that existing and new methods are used appropriately. Further work should develop and evaluate effective mechanisms of disseminating and implementing research findings by information transfer to dental educators and clinicians.

Decision Making↗

[Intravascular ultrasound].

An intravascular ultrasound (IVUS) is a new cardiological diagnostic technique which provides detailed cross-sectional images of the vessel wall, allows to investigate lumen, as well as structure of the coronary artery. This imaging technique has the unique potential to provide an image of atherosclerotic plaque, characterizes its composition, and severity of stenosis. "Soft" plaque, dense fibrous "hard" plaque, calcification and thrombosis have all been identified using IVUS. Intravascular ultrasound imaging has become a method for supporting interventional decision making by selecting patients who are to undergo Percutaneous Transluminal Coronary Angioplasty, Stent placement, Directional Coronary Atherectomy or Laser Angioplasty. Quality of measurements should allow more widespread use to guide interventional techniques. A series of IVUS imagings could assess the influence of plaque composition on mechanism of immediate lumen enlargement after revascularization. With utilization of IVUS guidance and high pressure balloon inflation--stent implantation could now be performed "optimally" and safely without anticoagulation. This original diagnostic tool is becoming a clinically useful adjunct to angiography in the assessment coronary arteries of heart transplant recipient and patients with higher risk of restenosis.

Humans↗

[MRI of the temporomandibular joint. Technique, results, indications].

An optimized examination protocol for the MRI evaluation of the temporomandibular joint (TMJ) is presented. The MRI protocol is based on an optimized coil technology, sequence design, and a programmed examination protocol for diagnostics of the TMJ. Depending on the clinical findings, MRI provides an all-in-one diagnostic protocol for the diagnosis of degenerative, inflammatory and tumorous lesions of the TMJ. MRI using an optimized examination protocol should become the primary examination protocol for diseases of the TMJ.

Adult↗

Optimal imaging in static telepathology.

Telepathology is the most recent addition to the diagnostic armory of the Pathologist. In spite of its relative limitations, as compared to Dynamic Telepathology, Static Telepathology has been widely accepted as a low-cost diagnostic and consultation tool, especially in those remote areas where expert opinions or second opinions on histopathology, cytopathology or haematopathology are not readily available to the reporting pathologist. For an accurate Telepathology performance, optimal quality of images is required. Knowledgeable use of available hardware and software have made it possible to produce high quality images, making Static Telepathology a reliable diagnostic tool.

Computers↗

Managing dysphagia in mentally retarded adults.

Mentally retarded adults with dysphagia are a chronically disabled population with high incidence of co-occurring gastrointestinal and respiratory disorders. Their ability to achieve and maintain optimal functional adequacy of swallow is dependent on medical and nutritional management of the disorder, as well as on improving the swallow itself. This paper presents a dysphagia management protocol for this population, which includes screening to identify at risk individuals, diagnostic evaluation of dysphagia, daily management to promote optimal mealtime function, and dysphagia therapy to improve underlying neuromotor competency and eating skills.

Adult↗

British Co-operative Clinical Group national survey on diagnostic issues surrounding genital herpes. MSSVD Special Interest Group on Genital Herpes and the British Co-operative Clinical Group.

OBJECTIVES: To investigate the current use of diagnostic methods for genital herpes simplex virus (HSV) infection, to determine how information from these tests influences clinical practice, and to identify areas for future guideline development within genitourinary medicine (GUM) clinics in the United Kingdom. METHODS: National survey of 173 consultants in UK GUM clinics. RESULTS: Completed questionnaires were returned by 146 (84%) consultants. Cell culture was the first line diagnostic method for 133 (91%) respondents, the remaining 13 (9%) used antigen detection tests. Typing of isolates (HSV-1 or HSV-2) was available in their local laboratory to 109 (75%) clinicians; however, less than two thirds routinely pass this information on to their patients. Although 74 (51%) respondents had access to serological diagnosis, the majority of methods described were non-specific; only three (2%) had access to type specific tests. Only 81 (56%) respondents frequently (> 90% of the time) recommend notification of recent sexual partners of genital herpes patients. CONCLUSIONS: While access to culture based diagnosis is widespread, type specific serology has limited availability. Information on typing of isolates as HSV-1 or 2, although available in three quarters of centres, is underutilized in counselling patients. As HSV type influences both clinical and subclinical reactivation rates and may also affect probability of transmission, this is an important omission. Future guidelines need to address the optimal use of viral typing and new diagnostic tests to optimise health gain; there is also a need for evidence based recommendations about partner notification in genital herpes.

Contact Tracing↗

A randomized prospective trial comparing unsedated esophagoscopy via transnasal and transoral routes using a 4-mm video endoscope with conventional endoscopy with sedation.

BACKGROUND AND STUDY AIMS: Unsedated upper endoscopy is an attractive alternative to conventional sedated endoscopy because it can reduce the cost, complications, and recovery time of the procedure. However, it has not gained widespread acceptance in the United States. A prototype 4-mm-diameter video esophagoscope is available. Our aims were to compare unsedated esophagoscopy using this 4-mm esophagoscope with conventional sedated endoscopy with regard to diagnostic accuracy and patient tolerance, to determine the optimal intubation route (transnasal vs. transoral), and to identify the predictors of tolerance of unsedated endoscopy. PATIENTS AND METHODS: Outpatients presenting for conventional endoscopy were randomized to undergo unsedated esophagoscopy by either the transnasal or the transoral route, followed by conventional endoscopy. The diagnostic findings, optical quality, and patient tolerance scores were assessed. RESULTS: A total of 137 patients were approached and 90 (65.6 %) were randomized to undergo esophagoscopy by the transnasal route (n = 44) or by the transoral route (n = 46) before undergoing conventional esophagoscopy. Patient tolerance of unsedated esophagoscopy was comparable to that of conventional endoscopy. The transnasal route was better tolerated than the transoral route, except with respect to pain, and 93.2 % in transnasal group and 91.3 % in transoral group were willing to have the procedure again. The diagnostic accuracy of endoscopy using the 4-mm video endoscope was similar to that of standard endoscopy. Patients who tolerated the procedure well had lower preprocedure anxiety scores (29 vs. 42.5, P = 0.021) and a higher body mass index (31.5 kg/m2 vs. 28 kg/m2, P = 0.029) than the other patients. CONCLUSIONS: Unsedated esophagoscopy with a 4-mm esophagoscope was well tolerated and has a level of diagnostic accuracy comparable to that of conventional endoscopy. Factors associated with good tolerance of unsedated esophagoscopy were low anxiety levels, high body mass index, and use of the transnasal route. Unsedated endoscopy may be offered to a selected group of patients based on these criteria.

Adult↗

An evaluation of current diagnostic tests for the detection of infectious salmon anaemia virus (ISAV) following experimental water-borne infection of Atlantic salmon, Salmo salar L.

Four commonly used diagnostic tests [reverse transcription polymerase chain reaction (RT-PCR), indirect fluorescent antibody test (IFAT), virus culture and light microscopy] were evaluated for their ability to detect infectious salmon anaemia virus (ISAV) or tissue pathology following experimental infection of Atlantic salmon. Fish were infected with ISAV by water-borne exposure which mimics the route of natural infection. Forty-five per cent of pre-clinical fish tested yielded positive results by RT-PCR for at least one of the organs tested (kidney, heart, gill, liver, blood). No significant difference was detected between organs in the number or time of first occurrence of positive result. Virus culture identified a total of 14% of pre-clinical fish as ISAV-infected. The presence of ISAV in heart tissue was particularly notable (13% of fish sampled) as was the inability to culture virus from spleen tissue. In the case of IFAT, 15% of fish sampled were positive, although tissue other than kidney proved unsuitable for use in this method. Only limited ISAV-specific pathology was detectable by histological examination of fish prior to the onset of clinical disease. These findings reveal important information regarding the optimal choice of both tissue sample and diagnostic test for the routine diagnosis of ISAV.

Anemia↗

Interpretation of diagnostic laboratory tests for severe acute respiratory syndrome: the Toronto experience.

BACKGROUND: An outbreak of severe acute respiratory syndrome (SARS) began in Canada in February 2003. The initial diagnosis of SARS was based on clinical and epidemiological criteria. During the outbreak, molecular and serologic tests for the SARS-associated coronavirus (SARS-CoV) became available. However, without a "gold standard," it was impossible to determine the usefulness of these tests. We describe how these tests were used during the first phase of the SARS outbreak in Toronto and offer some recommendations that may be useful if SARS returns. METHODS: We examined the results of all diagnostic laboratory tests used in 117 patients admitted to hospitals in Toronto who met the Health Canada criteria for suspect or probable SARS. Focusing on tests for SARS-CoV, we attempted to determine the optimal specimen types and timing of specimen collection. RESULTS: Diagnostic test results for SARS-CoV were available for 110 of the 117 patients. SARS-CoV was detected by means of reverse-transcriptase polymerase chain reaction (RT-PCR) in at least one specimen in 59 (54.1%) of 109 patients. Serologic test results of convalescent samples were positive in 50 (96.2%) of 52 patients for whom paired serum samples were collected during the acute and convalescent phases of the illness. Of the 110 patients, 78 (70.9%) had specimens that tested positive by means of RT-PCR, serologic testing or both methods. The proportion of RT-PCR test results that were positive was similar between patients who met the criteria for suspect SARS (50.8%, 95% confidence interval [CI] 38.4%-63.2%) and those who met the criteria for probable SARS (58.0%, 95% CI 44.2%-70.7%). SARS-CoV was detected in nasopharyngeal swabs in 33 (32.4%) of 102 patients, in stool specimens in 19 (63.3%) of 30 patients, and in specimens from the lower respiratory tract in 10 (58.8%) of 17 patients. INTERPRETATION: These findings suggest that the rapid diagnostic tests in use at the time of the initial outbreak lack sufficient sensitivity to be used clinically to rule out SARS. As tests for SARS-CoV continue to be optimized, evaluation of the clinical presentation and elucidation of a contact history must remain the cornerstone of SARS diagnosis. In patients with SARS, specimens taken from the lower respiratory tract and stool samples test positive by means of RT-PCR more often than do samples taken from other areas.

Adolescent↗

The sensitivity and specificity of direct immunofluorescence testing in disorders of mucous membranes.

BACKGROUND: Direct immunofluorescence testing is frequently used to diagnose inflammatory mucosal disorders, but its accuracy relative to histologic and clinical diagnosis has not been reported. OBJECTIVE: Our purpose was to compare diagnoses made on the basis of direct immunofluorescence, histologic features, and clinical impression and define optimal immunofluorescence criteria. METHODS: Direct immunofluorescence findings and diagnostic impressions for 500 unselected mucosal biopsy specimens were recorded, as were the histologic diagnosis, initial clinical impression, and final diagnosis made on the basis of all studies and follow-up. Sensitivity and specificity were calculated for each parameter by diagnosis and site. RESULTS: Direct immunofluorescence testing was superior for diagnosing pemphigus and pemphigoid and was slightly inferior to histologic evaluation for diagnosing lichen planus. Optimal criteria were IgG and C3 intercellular substance staining for pemphigus, linear C3 basement membrane zone deposits for pemphigoid, and shaggy fibrinogen basement membrane zone staining plus IgM cytoids for lichen planus. Direct immunofluorescence testing was diagnostic for several extraoral mucosal biopsy specimens. CONCLUSION: Direct immunofluorescence is a valuable diagnostic tool for diseases of the oral mucosa and other mucosal sites.

Complement C3↗