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PubMedDate not supplied

Intrauterine insemination with semen of oligozoospermic men: effectiveness of the continuous-step density gradient centrifugation technique.

There has been an argument as to whether the intrauterine insemination (IUI) substantially improves pregnancy rate in cases of oligoasthenozoospermia. The present study evaluates the efficiency of the IUI with the use of the life table analysis. The insemination was performed after washing and concentrating sperm using the continuous-step density gradient centrifugation reported in our previous papers. The number of IUI cycles studied was 222 (46 couples), in 13 cases of which pregnancy was established with a pregnancy rate of 28.3%. The cumulative probability of conception reached to 57% at the eleventh cycles of the IUI treatment. These results suggest that the potential efficiency of the IUI for the oligo-asthenozoospermic cases is high if the sperm is processed in a proper method.

Adult↗
PubMed1998

Effect of oxygen concentration in the gas atmosphere during in vitro insemination of bovine oocytes on the subsequent embryonic development in vitro.

In vitro matured bovine oocytes were co-incubated with sperm for 18 hr in a droplet of fertilization medium under a gas atmosphere of 5% CO2 with 5 or 20% O2. After removing the cumulus cells, they were fixed to examine their fertilization rate, or cultured for another 154 hr in a chemically defined medium under 5% O2 to determine their development to the blastocyst stage. There was no difference between the 5 and 20% O2 groups in the fertilization rate. However, the percentage of inseminated oocytes which developed to the blastocyst stage was higher when in vitro insemination was conducted under 5% O2 compared with that under 20% O2 (34.4 vs. 24.7%, P < 0.05).

Animals↗
PubMed2005

Onset and duration of luteal activity postpartum and their effect on first insemination conception rate in lactating dairy cows.

The incidence of different types of luteal activity postpartum and their effect on reproductive performance were studied in 21 postpartum dairy cows. Progesterone concentrations in defatted milk collected 3 times a week were determined by EIA. Reproductive tract examination was undertaken every other week postpartum. Body weight and body condition score (BCS) were measured before and after calving and the average 100-day milk yield was calculated. Nine (42.9%) cows had normal ovarian activity (first luteal activity < or = 50 days postpartum followed by regular cycles), 5 (23.8%) had prolonged luteal phase (PLP; ovarian cycle with luteal activity > or = 20 days pre-service) and in 7 (33.3%) cows the first luteal activity was shown later than 50 days postpartum (DOV). When compared with normal cows, both PLP and DOV had longer interval to first insemination (63.1 +/- 22.0 days versus 77.6 +/- 21.6 and 93.0 +/- 22.3 days, P<0.05 and P<0.01, respectively), lower first insemination conception rate (88.9% versus 0.0% and 57.1%, P<0.01 and P<0.05, respectively) and greater BCS loss (0.81 +/- 0.2 versus 1.05 +/- 0.21 and 1.04 +/- 0.10, respectively, P<0.01). Cows with PLP showed longer interval to uterine involution than normal and DOV groups (54.0 +/- 8.3 days versus 42.4 +/- 5.5 and 43.3 +/- 8.3 days, respectively, P<0.01) and higher 100-day milk yield (38.8 +/- 2.7 kg versus 33.6 +/- 4.7 and 29.9 +/- 6.1 kg, respectively, P<0.01). In conclusion, more than half of the cows had abnormal luteal activity postpartum, which adversely affected reproductive performance.

Analysis of Variance↗
PubMed1999

Effect of time interval from insemination to first cleavage on the developmental characteristics, sex ratio and pregnancy rate after transfer of bovine embryos.

In vitro produced bovine zygotes show substantial variation in the time required to complete the first cell cycle and in their in vitro development potential. A number of reports have highlighted the fact that the fastest developing embryos in vitro are most likely to be comparable with their in vivo counterparts. At 24 h after IVF, presumptive zygotes were cultured in droplets of synthetic oviduct fluid medium. Droplets were examined at regular intervals and all cleaved embryos at each time point were transferred into new droplets and cultured separately for the duration of the experiment. All uncleaved zygotes were returned to the incubator and re-examined at the successive time points until 48 h after insemination, at which time the remaining uncleaved oocytes were retained as a group. A representative number of day 7 blastocysts from zygotes that had cleaved by 30 or 36 h were transferred to synchronized recipients and pregnancy was diagnosed by ultrasonography at day 35. Glucose and glutamine metabolism was examined in zygotes and blastocysts and compared retrospectively with time of first cleavage. A representative number of blastocysts from each of the cleavage groups was sexed using PCR. Data were analysed by chi-squared and regression analysis. Development to the blastocyst stage decreased as the time from insemination to first cleavage increased (r = 0.97, P < 0.03). There was no difference in blastocyst hatching, number of blastocyst cells or pregnancy rate between the 30 and 36 h groups. The overall sex ratio was 62% males (n = 258, P < 0.0001) and was not different in the 30 and 36 h groups (61%, n = 155 versus 63%, n = 95, respectively). These results indicate that although time of first cleavage has a major influence on the probability of an embryo developing to the blastocyst stage, once that stage is attained, subsequent developmental characteristics are unrelated to the time of first cleavage.

Animals↗
PubMedDate not supplied

A double method sperm wash for artificial insemination.

Recently, there have been concerns regarding the presence of reactive oxygen species (ROS) produced during sperm processing for insemination. However, the sperm wash methods that yielded low ROS levels also had low sperm recovery after processing. The objective of this study was to compare sperm recovery after swim-up from pellet, overlay, and 2-layer Percoll wash methods with the recovery after the double method wash. The latter method consisted of a combination of 2 sperm wash methods, namely, the overlay and the Percoll method. Motile sperm were first collected through the overlay method. The leftover semen was then processed through the 2-layer Percoll method to scavenge motile sperm and the resultant pellet combined with the pellet from the overlay method. In this manner, the level of ROS was kept to a minimal, sperm recovery was improved, and a mixture of sperm with different surface properties was produced as a result of using different processing methods. The results indicated an improvement in sperm recovery and in total sperm motility in noncryopreserved sperm after using the double method wash when compared with the other wash methods. The study suggests that the double method wash is a feasible method for processing sperm for insemination.

Cell Separation↗
PubMed1988

[Milk ejection induced by PGF2 alpha during the first two months following insemination of cows. Application to pregnancy diagnosis].

Milk ejection after intrajugular injection of 256 micrograms of a synthetic PGF2 alpha analogue (Dinolytic, Upjohn) was measured every 2 days until D60 in 7 pregnant cows and until D25 in 5 non pregnant inseminated cows (trial 1). There was a very close correlation (r around + 0.98) between the values of the 3 parameters used to characterize milk ejection, i.e. amplitude of intramammary pressure deflection, surface area under the pressure curve, alveolar milk volume collected by a teat probe. In the two groups of animals, these values behaved similarly until D15 with a sudden increase between D2 and D7. From D15 they decreased abruptly in females where insemination had failed while they still remained at a high level until D25 (then decreasing until D60) in those presumed to be pregnant (fig. 2). These responses were assumed to result from the release of luteal oxytocin and it is suggested that a single injection of 256 micrograms of Dinolytic on D20, together with milk ejection control using a teat probe, would be a rapid, little expensive method for the farmer (without risks of luteolysis) (trial 2 and fig. 3) to distinguish between pregnant and non pregnant cows.

Animals↗
PubMedDate not supplied

[The effect of delayed insemination on fertilization in vitro and the development of human embryos].

Extracorporeal fertilization of 363 oocytes was applied in 148 patients with tubal infertility. The aspiration of the follicles was performed 36 to 38 hrs after the onset of the endogenous LH rise or 36 hrs after injection of hCG. Oocytes maturity was evaluated on the basis of the morphological appearance of the cumulus oophorus. The eggs were incubated in culture medium before insemination. The duration of delayed insemination had a marked effect on the proportion of fertilized oocytes and the development of embryos (X2 = 10.34, P = 0.05). After maturation of 1.5 to 3.5 hrs, minimal preovulatory oocytes were fertilized and developed to embryos. Maturation of 3.5 to 5.5 hrs increased the number of fertilized oocytes from 56% to 71.7% and the number of embryos from 50% to 55.1%. After 5.5 to 7.5 hrs of maturation significantly more fertilized oocytes (t = 2.4, P less than 0.02) and developed embryos (t = 2.41, P less than 0.02) were obtained. Preincubation of intermediary preovulatory or immature oocytes had no beneficial effect on the fertilization rate and the development of embryos.

Adult↗
PubMed1976

Artificial insemination using homologous semen: a review of 158 cases.

One hundred fifty-eight women underwent artificial insemination with homologous semen (AIH) in an attempt to achieve conception. Only 15 (9.5%) were successful. Women with anatomical abnormalities were not excluded from the study and they were less successful than the normal women, but results were disappointing in both groups. The most frequently recorded indication for AIH was decreased density or motility of the husband's sperm, but pregnancy occurred in only 2 of the 48 cases in which sperm count was consistently less than 50 X 10(6)/ml and in only 3 of the 63 cases in which sperm motility was consistently less than 60%. When several semen analyses revealed considerable fluctuation in semen quality, the chances for impregnation by natural means appeared to be greater than the likelihood of success with AIH. The procedure does not seem to compensate for diminished count or motility, does not seem to be of particular value in cases of unexplained infertility, and appears to be indicated only in very special cases, if at all.

Adult↗
PubMed1978

A longitudinal analysis of artificial insemination with donor semen.

A longitudinal analysis of artificial insemination with donor semen (AID) in 114 consecutive couples revealed a 37% over-all pregnancy rate. The 45 pregnancies occurred in 39 patients, and approximately 90% of these pregnancies occurred within six cycles of AID. Pregnancy rates were higher in the age group 25 to 30, in those who received only fresh semen, and in those with a history of a previous pregnancy. Lower pregnancy rates were observed in patients age 31 or older, in those with pelvic disease, and in those randomly receiving freshly thawed semen or fresh semen. The use of a vaginal device for retaining semen, the use of patient positioning, or the duration of infertility did not appear to affect the success rate. When surgically correctable pelvic disease was treated, a pregnancy rate of 22% was obtained.

Adolescent↗
PubMed1978

Intrauterine insemination of isolated motile sperm.

Semen from infertile males was filtered through liquid albumin, and sperm retrieved from the most distal fraction were used for intrauterine insemination. Compared with the ejaculate, the isolated specimens contained fewer sperm but an increased percentage of motile sperm. The fractions were also free of the debris (white cells, agglutinated sperm, immature forms, and particulate matter) commonly seen in the ejaculates. Despite the improvement in motility none of the 19 women who were treated for a total of 67 cycles became pregnant. It is possible that even the best sperm from a poor specimen may have intrinsic defects that interfere with their ability to fertilize.

Female↗
PubMed1980

Laparoscopic findings in twenty-five failures of artificial insemination.

The laparoscopic findings, treatment, and follow-up of 25 patients who failed artificial insemination donor (AID) were retrospectively evaluated. A high incidence (72%) of abnormal pelvic findings was encountered. Endometriosis was found in 60% and pelvic adhesions in 12% of the patients. The incidence of these two entities was not significantly different from their incidence in patients with unexplained infertility in the same institution (44% for endometriosis and 36% for adhesions). After surgical or medical treatment, a pregnancy rate of 22.2% (16.6% for endometriosis and 66.6% for pelvic adhesions) was achieved in patients with positive pelvic findings; in the group with negative pelvic findings, a pregnancy rate of 20% was achieved. The value of diagnostic laparoscopy in AID failures is discussed in the light of the findings presented and the relatively low rate of post-treatment pregnancy.

Adult↗
PubMed1981

Two hundred and seventy cases of artificial donor insemination: management and results.

Whenever male infertility is resistant to therapy, artificial donor insemination (AID) may be employed as a very efficient tool to provide the infertile couple with the desired child. Two hundred and seventy cases of AID were reviewed for the following information: indication for AID, age of the female partner, length and type of infertility, socioeconomic state, ovulatory pattern prior to and following initiation of the AID program, acceptance of the procedure by the male partner, and outcome of pregnancies. The over-all pregnancy rate was 85.2%. There were 159 live births, 2 perinatal deaths, 38 spontaneous abortions, 1 ectopic pregnancy, and 1 therapeutic abortion due to rubella infection. Forty patients discontinued treatment with or without notification. Treatment success was significantly affected by age of the woman, duration of infertility, socioeconomic state of the couple, and the husband's acceptance of the AID procedure. Of all pregnancies, 86.5% occurred within six treatment cycles. Repeated pregnancies required similar numbers of treatment courses per pregnancy.

Abortion, Incomplete↗
PubMed1981

Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH).

A method is described for the separation of motile sperm from semen by "migration' into a diluent, providing a large area of interface between the semen and the diluent and resulting in a good yield of motile sperm (58%), with a mean percentage of motility of 95%. The method provides a specimen free of seminal plasma and debris and suitable for intrauterine insemination in less than two hours. The motile fraction was used for AIH treatment of 20 couples with a mean of 4 years' infertility, where the female factor had been excluded and the male was asthenospermic. After a mean of six cycles, three pregnancies were achieved. Improvement of the percentage of motility alone did not, in this study, give a significant improvement in conception rates when compared with accepted cycles with the placebo.

Adult↗
PubMed1982

Female fertility and donor insemination.

Artificial insemination with donor semen (AID) frozen in 0.25-ml straws yielded an overall pregnancy rate of 64% in a series of 131 patients. The pregnancy rate was significantly higher in patients whose husbands were azoospermic (70%) than in women whose husbands were severely subfertile (48.8%) (P less than 0.02). Equivalent ages of patients as well as donor distribution between both groups were confirmed. This study brings evidence that patients turning to AID because of severe male subfertility represent a selected hypofertile population, from which the highly fertile females have disappeared through previous spontaneous conception.

Female↗
PubMed1982

Factors influencing the success of artificial insemination.

Factors influencing the probability of conception following artificial insemination with donor semen (AID) have been investigated in a series of 124 married females. Overall, 79 conceptions were achieved, for a cumulative rate of conception of 85.1% at the end of 1 year and an average fecundability of 15%, using life-table analysis. Women aged 30 or over or those with evidence of tubal or ovulatory problems had decreased probability of conception, although not to a significant extent. Women whose husbands were azoospermic had 20% fecundability in response to AID, significantly better than the 10% fecundability after AID observed in the women whose mates were oligospermic. Cycles during which AID was successful were significantly more likely to have had a positive postcoital test observed, compared with unsuccessful AID cycles.

Adult↗
PubMed1983

Pregnancy following use of the cervical cup for home artificial insemination utilizing homologous semen.

Sixty-one couples with infertility from 1 to 11 years were instructed in the use of the cervical cup for artificial insemination using homologous semen in the privacy of their own homes. There have been 36 reported pregnancies in 32 of these couples. Among women with primary infertility, the pregnancy rate was 43%; it was 67% for those with secondary infertility and 53% overall. Sperm counts and percent motility, as well as postcoital test results, however, failed to be indicative of eventual ability to conceive. Regardless, among couples with documented infertility, this method provided over half of the couples with at least one pregnancy. Additionally, the technique is simple, inexpensive, without significant risk or discomfort, and can be carried out by a couple at their convenience and in privacy.

Female↗
PubMedDate not supplied

Value of sperm morphology assessed by strict criteria for prediction of the outcome of artificial (intrauterine) insemination.

The purpose of this study was to investigate the relationship between sperm parameters and the outcome of artificial (intrauterine) insemination (IUI). One hundred and twenty-six patients undergoing 395 consecutive IUI cycles in a 1-year period in our institution were studied. In all cases, controlled ovarian stimulation and hCG-timed IUI were performed, followed by progesterone supplementation of the luteal phase. In 86 patients, (243 cycles) the husband's sperm was utilized, whereas in 40 couples (152 cycles), donor sperm was used due to severe male factor infertility. Among the sperm parameters of the original ejaculate, % normal morphology (assessed by strict criteria) was the most significant predictor of pregnancy (stepwise regression analysis, P = 0.003). Using logistic regression, morphology was the best predictor of pregnancy (r = 0.12); linearity of movement significantly enhanced the predictive value of morphology alone (r = 0.17, P = 0.004). Overall, the pregnancy rates were significantly higher (P = 0.01) in cases with > or = 14% normal morphology (15% per cycle) compared to cases with < 14% normal morphology (7% per cycle). This threshold level for morphology is in agreement with previously published IVF results. These results underscore the significance of sperm morphology assessed by strict criteria as a predictor of pregnancy outcome in the IUI setting.

Adult↗