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Rhesus monkey (Macaca mulatta) complex learning skills reassessed.

Results from three experiments on basic learning and transfer in rhesus monkeys (Macaca mulatta) are reported in which fully automated testing paradigms, afforded by the Language Research Center's Computerized Test System (LRC-CTS), were employed. Performance levels for discrimination learning set, transfer index, and mediational-learning testing were uniformly higher than was predicted from the literature, in contrast to previous reports of compromised learning under similar conditions (automated apparatus, planimetric stimuli, spatial discontiguity between stimuli and response loci). Analyses reveal relatively advanced learning set performance, transfer-index ratios, and positive transfer of learning even at stringent criterion levels. Moreover, the data suggest that rhesus monkeys tested in these experiments exhibit mediational instead of associative learning strategies, as do great apes and in contrast to previous reports of rhesus learning. We argue that the LRC-CTS enhances learning by nonhuman primate subjects, obviating those factors, reported in the literature from experiments in which manual or other automated systems were employed, that compromise learning.

Animals↗

Lesions of nucleus accumbens disrupt learning about aversive outcomes.

Nucleus accumbens (NAcc) is critical for encoding and using information regarding the learned significance of cues predictive of reward. However, its role in processing information about cues predictive of aversive outcomes is less well studied. Here, we examined the effects of NAcc lesions in an odor-guided discrimination task in which rats use odor cues predictive of either appetitive or aversive outcomes to guide responding. Rats with sham or neurotoxic lesions of NAcc were trained on a series of two-odor discrimination problems. Performance on each problem was assessed by monitoring accuracy of choice behavior and by measuring latency to respond for fluid reinforcement after odor sampling. After acquisition of four problems, rats were trained on serial reversals of the final problem. Rats with NAcc lesions exhibited normal choice performance relative to controls on both acquisition and reversal of the discrimination problems (indeed, lesioned rats exhibited a mild facilitation on the first discrimination problem). Despite normal choice performance, however, lesioned rats failed to show normal changes in response latency during discrimination learning, particularly on trials involving the aversive outcome. These findings are consistent with a deficit in processing cue-outcome associations. These results are compared with those obtained from studies of basolateral amygdala and orbitofrontal cortex lesions in this task and suggest that NAcc integrates the motivational value of both appetitive and aversive cues to bias or modulate the vigor of subsequent responding.

Animals↗

Amygdalectomy and disconnection in visual learning for auditory secondary reinforcement by monkeys.

Nine monkeys in 3 groups took part in an experiment on visual discrimination learning set in an automatic apparatus. Each new visual discrimination problem was solved using auditory secondary reinforcers. Primary food reinforcement was delivered only after a new problem had been solved to a criterion, and the problem was then replaced by a new one; thus, within-problem learning relied purely on secondary and not on primary reinforcement, but the secondary reinforcers were associated with primary reinforcement. Bilateral amygdalectomy severely retarded within-problem learning. Disconnection of the amygdala from auditory input, by crossed unilateral lesions of amygdala and of auditory cortex combined with forebrain commissurotomy, had a similar effect to that of bilateral amygdalectomy. Disconnection of the amygdala from visual association cortex left learning unimpaired. Thus, for normal performance in this task, interaction of the amygdala with the sensory modality of the secondary reinforcer was essential, but interaction of the amygdala with the sensory modality of the discriminative stimuli was not necessary. It was concluded that the amygdala is involved in associating stimuli with the primary reinforcing attributes of food reward, and not with its other attributes.

Amygdala↗

Neuromagnetic fields reveal cortical plasticity when learning an auditory discrimination task.

Auditory evoked neuromagnetic fields of the primary and association auditory cortices were recorded while subjects learned to discriminate small differences in frequency and intensity between two consecutive tones. When discrimination was no better than chance, evoked field patterns across the scalp manifested no significant differences between correct and incorrect responses. However, when performance was correct on at least 75% of the trials, the spatial pattern of magnetic field differed significantly between correct and incorrect responses during the first 70 ms following the onset of the second tone. In this respect, the magnetic field pattern predicted when the subject would make an incorrect judgment more than 100 ms prior to indicating the judgment by a button press. One subject improved discrimination for much smaller differences between stimuli after 200 h of training. Evidence of cortical plasticity with improved discrimination is provided by an accompanying decrease of the relative magnetic field amplitude of the 100 ms response components in the primary and association auditory cortices.

Acoustic Stimulation↗

Narcotic cuing and analgesic activity of narcotic analgesics: associative and dissociative characteristics.

By using a discrete-trial, two-lever, food-reinforced discrimination learning paradigm, rats were trained to discriminate the narcotic analgesic fentanyl (0.05 mg/kg) from saline. Stimulus generalization experiments with lower fentanyl doses (0.0025 to 0.02 mg/kg) were carried out to generate individual threshold doses. The latter were compared with the sensitivity of the same rats to the analgesic effect of fentanyl, and it was found that there is no correlation between these two sets of data. In a time-effect experiment, the duration of fentanyl's cuing effect was compared with that of its analgesic effect, and it was found that the time-effect characteristics of the narcotic cue are similar to those of analgesia. Again, however, there was no correlation between the duration of both effects within the same group of animals. The results further deliniate the associative and dissociative characteristics of the narcotic cue and narcotic analgesia.

Analgesics↗

Appetitive learning in snails shows characteristics of conditioning in vertebrates.

The snail Lymnaea stagnalis can be rapidly conditioned to produce feeding response to a previously neutral tactile stimulus, repeatedly and specifically paired with positive reinforcement (food). The conditioned response has many of the same characteristics seen in appetitive learning in vertebrates. The relative simplicity of the molluscan CNS and the detailed knowledge of the neural circuitry underlying feeding in Lymnaea will facilitate the neurophysiological analysis of appetitive learning as well as stimulus generalization, discriminative learning and generalization of extinction all described in the present paper.

Animals↗

Teaching relational discrimination to individuals with mental retardation: some problems and possible solutions.

The problem of teaching relational discriminations to people with mental retardation was examined. The limitations of several commonly used teaching procedures were discussed and alternative approaches that derive from studies of simple-discrimination learning were described. Results of two preliminary studies were summarized that demonstrate the feasibility of these approaches and suggest new methods for teaching relational discriminations to individuals with developmental limitations.

Attention↗

omega-Conotoxin GVIA disrupts memory formation in the day-old chick.

Calcium channel antagonists have previously been shown to alleviate age-associated reductions in memory acquisition and enhance retention for a number of different tasks. We have investigated the effects of pretraining injections of nifedipine, nimodipine, and amlodipine (L-type calcium channel antagonists) and omega-conotoxin GVIA (an N-type calcium channel antagonist) on passive avoidance and visual discrimination learning in day-old Ross 1 chunky chicks. None of the L-type calcium channel antagonists at any time or dose tested caused amnesia for the passive avoidance task or enhanced weak learning, and nifedipine did not affect acquisition or retention of the visual discrimination task. However, pretraining bilateral intracerebral injections of 1.25 pmole/hemisphere omega-conotoxin GVIA produced amnesia for the passive avoidance task in chicks tested 30 min or 3 h after training. The same dose of omega-conotoxin GVIA significantly reduced the rate of acquisition of the visual discrimination task, but did not affect retention of the task when tested 30 min or 3 h after training. These data indicate that calcium uptake via presynaptic N-type omega-conotoxin-sensitive but not L-type dihydropyridine-sensitive calcium channels is necessary for memory formation in young chicks.

Age Factors↗

Lack of correlation between cortical levels of choline acetyltransferase and learning scores in rats with globus pallidus lesions.

Previous work has shown that rats with lesions of the globus pallidus (GP) exhibit a generalized learning impairment. Data are presented suggesting that this impairment is not due to inadvertent damage to the nucleus basalis magnocellularis. Rats with GP lesions evidenced a significant visual discrimination learning loss and a significant reduction in cortical choline acetyltransferase (ChAT) activity. However, there was no significant correlation between the severity of the learning loss and the amount of reduction of cortical ChAT activity.

Animals↗

Learning of imitate in infancy.

Theories of imitation learning are examined regarding their account of how the infant acquires the ability to emit a response which resembles a response previously exhibited by another. Each theory suffers from 1 or more of the following problems: logical inconsistency, incompleteness, and lack of empirical support. 3 of the views (associative thesis, discriminative learning thesis, and acquired value of the relational stimulus of similarity) are not mutually exclusive, and, therefore, they could be included in a theory of learning to imitate. However, none of these learning theses deals with the fact that imitation requires the infant to consistently abstract similar features from stimuli which differ on various dimensions. Hence, consideration is given to the role of cognition in a theory of learning to imitate.

Association Learning↗

Ontogenetic and phylogenetic shifts in the cognitive representation of the conditioned response.

The retention of the conditioned response was tested in a retroactive interference paradigm using subjects from kindergarten, Grades 3 and 6, and college. The conditioned stimuli (CS) were either a tone or light, and the unconditioned stimuli (US) were vibratory tactual stimulations delivered to either the right or left index finger which produced a withdrawal response. Original AB learning consisted of pairing one of the CSs with one of the USs (e.g., tone-left). Interpolated CD learning consisted of pairing the other CS with the other US (e.g., light-right). When the first stimulus was presented alone to test for retroactive interference, the college students gave the response associated with it during original learning, the B response, but the kindergartners and third graders gave the D response, which was never associated with the CS during training. A second experiment used avoidance conditioning instructions and replicated these results while including rest-control and AD groups. These data suggest that what is learned by children in simple conditioning paradigms is different than what is learned by adults in the same paradigms. The results are discussed in terms of the following two hypotheses: (a) Russian theories of conditioning and extensions of Tighe and Tighe's and Kendler's theories of the development of perception and discrimination learning in children, and (b) theories of development of separability in perceptual development.

Adolescent↗

Investigation of narcotics and antitussives using drug discrimination techniques.

Rats learned drug discriminations in a shock-escape T-maze task. They were trained to turn right in the maze following injection of a drug (D) and left when no injection (N) was given. Number of training sessions before criterion performance (STC) was used to indicate degree of discriminability of the training drug. STC decreased monotonically as dosage increased, and reached a minimum of 3 to 26 with various agonists. Most agonists were not highly discriminable. Daily maintenance injections of morphine, 200 to 600 mg/kg, increased the STC of morphine, 15 mg/kg, significantly, but complete tolerance to discriminable drug actions was not observed. After rats discriminated D vs. N, they were tested with novel drugs to determine which would elicit D choices. Most morphine-like agonists substituted for one another during substitution tests; the tested agonists included alphaprodine, codeine, fentanyl, heroin, meperidine, methadone, morphine, piminodine and propoxyphene. In a few instances, one of these agonists failed to substitute for another. Naloxone and naltrexone antagonized the discriminable effects of morphine. Cyclazocine, levallorphan, naltrexone, dextromethorphan, ethoheptazine and the narcotic agonists did not substitute for one another, suggesting that six dissimilar discriminable effects were produced by these drugs.

Analgesics, Opioid↗

The effects of post-weaning environment, biological dam, and nursing dam on feeding neophobia, open field activity, and learning.

Using rats in a cross-fostering design we examined the effects of pre- and post-weaning rearing environments on feeding neophobia, open field activity, runway training, and visual discrimination learning. Fostering had no effect on the offspring behaviors. The animals reared, post-weaning, in an enriched environment consumed more food in a novel situation, were less active in the open field, and learned the first of two discrimination tasks faster than did the animals reared in the standard condition. Inter-correlations among these measures were nonsignificant, suggesting that the effect of environment on learning cannot be reduced to temperamental factors. The deficit in learning resulting from rearing in standard environment does not, however, appear to be irreversible; animals reared in the standard condition reached performance levels on the second discrimination task characteristic of those reared in the enriched environment.

Animals↗

DARPP-32 knockout mice exhibit impaired reversal learning in a discriminated operant task.

The current study was conducted to examine the performance of mice with a targeted deletion of the gene for DARPP-32 in a discriminated operant task using food reinforcement. DARPP-32 plays a central role in regulating the efficacy of dopaminergic neurotransmission. Initially, wild-type and DARPP-32 knockout mice were trained to nose-poke for food on a continuous reinforcement schedule. The minimum response requirement was increased every 5 days until the animals were responding on an FR-15 schedule of reinforcement. At the completion of extensive operant training, reversal learning was assessed. Wild-type and DARPP-32 knockout mice exhibited equivalent performance during acquisition of this task, with both groups increasing operant responding as the schedule of reinforcement was raised. However, significant differences in discrimination learning were observed during the reversal phase, with DARPP-32 knockout mice requiring significantly more trials to reach criterion than wild-type controls. These results provide evidence for a functional role of DARPP-32 in the mediation of processes underlying learning and memory.

Animals↗

More learned irrelevance than perseveration errors in rule shifting in healthy subjects.

The present experiments examined the extent to which two possible sources of error affect healthy subjects' performance in a rule-shift task. All 115 participants first received a discrimination learning task, in which a pair of different visual stimuli was presented on each trial, one of which had to be identified as 'correct.' Each stimulus varied in two dimensions: a task-relevant and a task-irrelevant dimension. Feedback on correctness was given after each choice. After eight successive correct choices, the nature of the task-relevant dimension changed: the post-shift learning phase. Two types of error can occur in this phase: continued responding to the former relevant, but now irrelevant, dimension, a perseverative error, and non-responding to the former irrelevant, but now relevant, dimension, an error due to learned irrelevance. Different groups received a post-shift task in which none, one, or both of these two types of error could affect performance. The number of incorrect choices in the post-shift phase was significantly affected by learned-irrelevance errors but not by perseverative errors. An associative-learning model incorporating feedback-induced changes in both associative strength and saliency of the elements comprising the stimuli can explain these results.

Adolescent↗

The effects of anticholinergic drugs on delayed time discrimination performance in rats.

To investigate the effects of the muscarinic antagonist scopolamine and the nicotinic antagonist mecamylamine on time discrimination and short-term memory, rats were trained on a delayed conditional time discrimination task until performance stabilized. In a two-lever operant chamber, pressing one lever was correct after the presentation of a stimulus light for 2 seconds (SD short); and pressing the other lever was correct after presentation of a stimulus light for 8 seconds (SD long). Scopolamine (0.06 mg, 0.25 mg, and 1.0 mg/kg) attenuated performance in a dose-dependent manner. Furthermore, the drug decreased nose-pokes (an activity necessary to trigger the presentation of the discriminative stimuli and the presentation of the response levers), and increased response delay (time from opportunity to lever press to actual lever press). Performance attenuating effects of mecamylamine in the time discrimination task did not appear unless high doses (8 mg/kg) of the drug were given. With increasing delays between 0 and 8 seconds, animals injected with saline developed a bias towards reporting the occurrence of the SD long, independent of the actual stimulus presented. A reversal of this bias was seen in animals injected with scopolamine; they more often reported the occurrence of the SD short. Our results support a role for muscarinic receptors in discrimination learning, attention, and time estimation.

Animals↗

Evidence of complementary afterimages in the pigeon.

Key pecking of pigeons was reinforced on a variable-interval schedule when ambient illumination came from a green light, but not when it came from a red, yellow, or white light. The different hues were randomly presented for periods of 30 sec each, with the restriction that white never followed red. After discriminative control was established, the pigeons were tested with the same procedure used during training, except that white sometimes followed red. Significantly more responses were made during white-following-red than during white following either green or yellow. These findings indicate that, in changing from red to white, complementary afterimages can be induced in pigeons for a brief period of time. By providing behavioral evidence for afterimages in the pigeon, this technique may be useful to research in comparative neurophysiology, animal discrimination learning, and theories of color vision.

Afterimage↗

Learned behavior of Rhesus monkeys following neonatal bilateral prefrontal lobotomy.

Bilateral ablation of dorsolateral frontal cortex in the newborn animal failed to produce the frontal lobe syndrome typical of the adolescent and adult stages. The crucial tests (delayed response, string tests, and discrimination learning) were performed 3 to 4 months postoperatively. At this time, no significant differences between normal animals and animals with prefrontal lesions were found.

Animals↗