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On the nurse cell and the spermatozeugma in Littorina sitkana.

Nurse cells develop from diploid cells in the testis. Each cell undergoes a reduction division which leaves the nucleus with half the volume of a normal diploid cell. They send out pseudopodia which form desmosome-like junctions with developing spermatids. The nurse cells detach from the testicular wall, their nuclei degenerate and secretion droplets form in the cytoplasm. The pseudopodia are drawn in as the cytoplasmic secretions swell and the nurse cell becomes spherical. The eupyrene sperm become grouped unilaterally and at this stage are attached to the nurse cell by only the tips of their acrosomes. At maturity the nurse cells with their clumps of attached eupyrene sperm (spermatozeugmata) are released from the testis via ducts into the seminal vesicles,where they are stored prior to copulation. Nurse cells serve similar functions to those of apyrene sperm which are common among the Molluscs. We believe that the nurse cell and apyrene sperm are homologous.

Acrosome↗

Validation of the in vivo somatic mutation method in the mouse as a prescreen for germinal point mutations.

The in-vivo somatic mutation method developed by us in an earlier X-ray experiment was tested for its usefulness in chemical mutagenesis work, specifically in the prescreening for germinal point mutations. In order to explore possible parallelisms, the 7 compounds chosen for study, as well as the genetic markers used, were those with which large-scale specific-locus mutation-rate experiments in germcells had been conducted in the past or were in progress. From 1--3 dose levels were tested for each compound. On day 10 1/4 after copulation of C57BL females with T males, a single injection of the test compound was administered, and about 2000 offspring altogether were subsequently scored for survival, morphology, and presence of spots of various types. In accordance with our earlier results we found 3 types of spots: white near midline ventral spots (WMVS) which probably result from killing of melanocyte precursor cells; spots resulting from misdifferentiation; and the remainder, which probably result from expression of the recessive by one of several mechanisms (RS). Induction of teratogenic effects, which were stage-specific rather than agent-specific, generally paralleled induction of WMVS's. Both are interpreted as resulting from cell killing. Induction of RS's did not always parallel induction of WMVS's, but roughly paralleled relative frequencies of specific-locus mutations induced in spermatogonia by the same compounds. Even though the in vivo somatic-mutation method probably detects genetic changes additional to point mutations, the results indicate that it may be a useful prescreen for germinal specific-locus mutations, provided care is taken to distinguish between the 3 types of spots, only one of which (RS) is indicative of expression of the recessive.

Animals↗

Detection of chloroplast DNA by using fluorescent monoclonal anti-bromodeoxyuridine antibody and analysis of its fate during zygote formation in Chlamydomonas reinhardtii.

A monoclonal anti-bromodeoxyuridine antibody conjugated to fluorescein was used to detect the chloroplast nucleoids after specific incorporation of bromodeoxyuridine (BUdR) into the chloroplast DNA of Chlamydomonas reinhardtii. The incorporation of BUdR was enhanced by simultaneous treatment with fluorodeoxyuridine (FUdR). The method was applied to analyze the fate of chloroplast DNA in zygotes resulting from mating between BUdR-treated gametes (mt+ or mt-) and untreated gametes of opposite mating-type. In crosses between wild-type strains, the nucleoids of mt+ origin remained in the large majority of zygotes whereas those of mt- origin most often disappeared within the first hours following copulation. In crosses of the type mat-3 mt+ x wild-type mt- (the mat-3 mutation permits a high transmission of chloroplast genes from the mt- parent), the nucleoids of mt- origin were generally not eliminated which indicates that the mat-3 mutation prevents the selective destruction of paternal chloroplast DNA in the zygote.

Antibodies, Monoclonal↗

Sexual reproduction in the monogenean Diclidophora merlangi: tissue penetration by sperms.

Copulation between pairs of adult Diclidophora merlangi detached from the host was frequently observed. The spined penis of one animal always attaches to a second worm at a latero-ventral position posterior to the genital openings. There is no vagina. The sperms travel between the cells of the recipient to reach the seminal receptacle. The ultrastructure of the sperm is described. Positions of adult D. merlangi on the gills of the host would facilitate pairing for sperm transfer.

Animals↗

Influence of concanavalin a on autolysis of gametes from Chlamydomonas reinhardii.

The phytohemagglutinin concanavalin A inhibited zygote formation of Chlamydomonas reinhardii. 15--50 mug lectin/ml not only interfered with the mating reaction, but also with cell wall lysis of gametes and zoospores in a crude autolysin preparation gained from copulating gametes. Further, the structure of cell walls shed into the medium after autolysis in the course of the mating reaction and after lysis "from without" in the crude autolysin preparation was stabilized by Con A. Therefore, it must be assumed that the lectin inhibited zygote formation of C. reinhardii by interfering with autolysis of the cell walls of the gametes. Though Con A inhibited the lytic processes of C. reinhardii, an activation of the autolytic system in theta gametes by the lectin was found to compete with its inhibitory reaction. Con A induced autolysis of theta gametes was dependent on adherence of the cells by their flagella to the surface of the culture vessel or the liquid medium and did not occur in cultures stirred by rotation. The interferences of Con A with the autolytic serum of C. rienhardii were inhibited by methyl-alpha-D-mannopyrano-side and to a lesser degree by glucose, indicating that the carbohydrate binding sites of the lectin were involved in its reactions with the cells.

Binding, Competitive↗

Effects of caffeine and various xanthines on hornets and bees.

The effect of caffeine was assessed on Vespa orientalis hornets maintained either in sealed breeding boxes or as entire colonies free to forage, and also on Apis mellifera bees within their hives. In a number of instances the hornets were also used to study the effect of various bodily extracts of queen hornets and of the following xanthines: Purine; hypoxanthine; uric acid; theophylline; and theobromine. The studied materials were found to exert an effect on three categories of activities: (1) Motor motility, flight, and construction; (2) sensory response to light (retinal and extraretinal), noise, irritability, orientation; and (3) physiological changes in appetite, copulation, oviposition, hibernation, resistance to cold, and longevity. Up to a point the produced effects were reversible. Throughout the period of experimentation the test insects did not show signs of tolerance or addiction towards caffeine.

Animals↗

The effects of neonatal capsaicin treatment on growth and subsequent reproductive function in the rat.

The results of the present study describe the following effects of neonatal capsaicin treatment on growth and reproductive function in the rat. Neonatal capsaicin treatment in the rat resulted in a retardation of body growth through adulthood most pronounced at 35 days of age. Capsaicin-treated rats had normal estrous cycles, but mated significantly less frequently than age-matched controls. Confirmed matings in capsaicin-treated female rats resulted in significantly fewer pregnancies compared to controls. Male rats treated with capsaicin as neonates produced significantly fewer pregnancies when mated with untreated females compared to controls. Ovulation, sperm transport, and fertilization occur normally in capsaicin-treated rats. The decidual response was markedly diminished in mated capsaicin-treated rats. These findings led to the conclusion that the neurohormonal reflex, which is normally initiated through the stimulation of the cervix by copulation, and which ultimately stimulates secretion of pituitary luteotropic hormones, did not occur in capsaicin-treated rats. Thus, ovarian progesterone secretion was not facilitated resulting in inadequate hormonal support for implantation, pregnancy or pseudo-pregnancy. These data further suggest that the sensory limb of this neurohormonal reflex may consist of capsaicin-sensitive, possibly substance P containing, primary afferent fibers.

Animals↗

Cyclophosphamide effects on fetal mouse cephalic acetylcholinesterase.

Pregnant C3H mice were exposed to a teratogenic dose of cyclophosphamide (CP, 20 mg/kg body weight) on the 10th day after copulation. Embryos or fetuses were examined 2-9 days later for gross abnormalities, weighed, and assayed for cephalic acetylcholinesterase (EC 3.1.1.7, AChE) activity, and haemoglobin content. In embryos examined 2 days after CP administration, fetal weight and brain weight were less than controls, but cephalic AChE was higher (p less than 0.001). The higher cephalic AChE was associated with a higher absorbance for haemoglobin, although at 14 days there was no apparent difference in the cephalic AChE and absorbance for haemoglobin, compared with controls. At 19 days, fetal weight, brain weight and brain AChE activity were less than controls (p less than 0.001 in each case). The raised cephalic AChE activity in 12-day embryos is explained by the presence of blood, which appears to have resorbed by day 14, whereas the reduced brain AChE activity in 19-day fetuses is accounted for by the growth inhibitory effects of the drug.

Acetylcholinesterase↗

Scanning electron microscopy of Ancylostoma ceylanicum and its site of attachment to the small intestinal mucosa of the dog.

The relationship between the hookworm Ancylostoma ceylanicum and the host intestinal mucosa in situ has been examined by using an in vivo fixation technique and scanning electron microscopy. Heads of the adult worms were buried deeply in the mucosa of the dog's small bowel. At some sites of attachment there was severe atrophy and ulceration of the immediately surrounding villi. Superficial deposits of fibrillar material with many enmeshed erythrocytes were associated with these attachment sites. When adult worms became removed after fixation, large numbers of erythrocytes could be seen at the base of the ulcers. Examination of the morphological details of adult A. ceylanicum revealed the characteristic mouthparts of large outer ventral teeth and smaller inner teeth. The distance between adjacent transverse cuticular striations was measured as 5.2 microns. The wide separation of the externolateral ray from the closely related mediolateral and posterolateral rays was illustrated together with the functi characteristic mouthparts of large outer ventral teeth and smaller inner teeth. The distance between adjacent transverse cuticular striations was measured as 5.2 microns. The wide separation of the externolateral ray from the closely related mediolateral and posterolateral rays was illustrated together with the functi characteristic mouthparts of large outer ventral teeth and smaller inner teeth. The distance between adjacent transverse cuticular striations was measured as 5.2 microns. The wide separation of the externolateral ray from the closely related mediolateral and posterolateral rays was illustrated together with the functional relationship of male and female copulating worms.

Ancylostoma↗

Studies of esterase 6 in Drosophila melanogaster. VII. Remating times of females inseminated by males having active or null alleles.

Esterase 6 (EST 6) in Drosophila melanogaster is a male reproductive enzyme transferred to females as a component of the seminal fluid [Richmond, R. C., Gilbert, D. G., Sheehan, K. B., Gromko, M. H., and Butterworth, F. W. (1980). Science 207:1483-1485]. Here we report investigation into the relation between EST 6 and remating by females. EST 6 activity in a strain selected for decreased time to remating is increased over control levels. Inseminated females remated to males carrying null or active alleles show no differences in the timing of remating, However, females inseminated by EST 6-active males remate significantly sooner than females inseminated by null males. Interrupted copulation experiments demonstrate that the remating effect is not due to EST 6 alone but requires other components of the ejaculate. Other evidence suggests that sperm stored in the ventral receptacle respond to EST 6 levels and control remating time. As the first mate of a female who will remate, null-EST 6 males have, under laboratory conditions, a significantly higher fitness than males carrying active alleles. Thus the absence of null alleles of EST 6 in natural populations presents a dilemma suggesting that the remating effect of EST 6 may be balanced by other effects on reproduction.

Animals↗

Male-male interactions and mating kinetics in Drosophila.

Male-male interaction (K) has been estimated from data on the attrition rate of virgin females per minute in a study of the mating kinetics in Drosophila. K is expressed as the time males expend on other males relative to that expended while searching for, courting, and copulating with females. The value of K in these studies ranged from 0 (approximately) to .695; it was affected both by strain (sepia or ebony D. melanogaster and wild-type D. simulans) and by size of the mating chamber. Host-parasitoid models of ecologists appear to be appropriate for examining mating kinetics in Drosophila.

Analysis of Variance↗

Analysis of truncated distributions: mating speed in Drosophila melanogaster.

The distribution of mating speeds in wild-type Drosophila melanogaster is shown to be log normal. The analysis of mating speeds by methods for truncated distributions is validated, and unbiased estimates of the mean mating speed, the variance of mating speed, and the proportion of flies capable of ever mating are produced. In pair matings, not all pairs are capable of copulating, given even a 7-day mating period.

Animals↗

Courtship behavior of Drosophila genetically or surgically deprived of basiconic sensilla.

The lack of basiconic antennal sensilla in the mutant lozenge was used to assess the role of these olfactory receptors in the courtship behavior of Drosophila melanogaster. Under normal light conditions, lozenge males courted virgin females much less than wild-type males did. However, when visual courtship stimuli were eliminated by studying behavior under dim red light, the two kinds of males courted individual wild-type virgin females with the same intensity, and the latency to copulation was similar. Also, no difference in courtship vigor was observed if the two kinds of males were paired in red light with a mated female. These data suggest that antennal basiconic sensilla are important for neither the perception of the attraction pheromone(s) of virgin females nor the inhibitory pheromone(s) of mated females. Similar assays with males deprived of maxillary palps make it unlikely that the basiconic-like sensilla on these appendages are needed to perceive the attraction pheromones. However, the unexpectedly high courtship activity of palp-deprived males toward mated females suggests that basiconic-like maxillary sensilla may be receptors of inhibitory female compounds.

Animals↗

Relative male age, fertility, and competitive mating success in Drosophila melanogaster.

Female choice experiments were used to investigate the effect of relative male age on mating success in D. melanogaster. Experiments were conducted with a Canton-S (CS) strain, in which two virgin males of different ages (2, 4, or 8 days old) were offered to virgin females. Older males were found to be more successful under competitive conditions in another group of experiments, vermilion (v) males of different ages competed with CS males of different ages. The competitive success of v males was found to increase with their relative age. Male fertility at 2, 4, and 8 days of age was documented for both male genotypes mated with CS females. CS males fathered more offspring per copulation than v males, and the fertility of all males was found to increase with age. Discussion focuses on the changes in male mating success and fertility with age and genotype.

Age Factors↗

Portions of the central nervous system controlling reproductive behavior in Drosophila melanogaster.

Drosophila melanogaster sex mosaics were tested in their courtship interactions with females and then with males. The distribution of genetically male and female tissues in each mosaic was determined with respect to an external cuticle marker and an internal enzyme marker. Performance of malelike courtship was correlated with the genotype of various tissues, with special attention being paid to the genotypes of head and thoracic ganglia. Male tissue in the left or right dorsal brain is necessary and nearly always sufficient to trigger early courtship actions--following of females and wing extension at them--but male tissue in both the dorsal brain and thoracic ganglia is necessary for attempted copulation to occur. Female tissue on or in the abdomen is nearly always necessary and sufficient for a mosaic to be courted by a male.

Animals↗

Transmission of Demodex flagellurus (Acari: Demodicidae) in the house mouse, Mus musculus, under laboratory conditions.

Transmission of Demodex flagellurus Bukva, 1985 inhabiting the preputial and clitoral glands of the house mouse, Mus musculus L., was studied in mating experiments using pairs of infested, feral X mite-free, white laboratory mice. Spontaneous transfer of mites between sexually active hosts of the opposite sex, presumably through copulation, is reported for the first time for the hair-follicle mites under controlled conditions. Transfer of mites from host mother to her progeny was also proven. A comparatively low transmission effectiveness was observed in both modes of transmission, being 9.5% in adult-to-adult transmission and 14.8% in mother-to-young transmission.

Animals↗

Effects of chlordiazepoxide, oxazepam, chlorpromazine, and d-amphetamine on sexual responses in male and female hamsters.

The acute effects on sexual behavior of oxazepam (16--64 mg/kg), chlordiazepoxide (8--64 mg/kg), chlorpromazine (2--8 mg/kg), and d-amphetamine (0.8--3.2 mg/kg) were examined in intact male and female golden hamsters (Mesocricetus auratus). Intraperitoneal injections were given 45 min before the first behavioral test. In 10-min tests, lordosis was observed in estrous females both before and after copulation, and mounts, intromissions, and ejaculations were observed in males. Dose-response related decrements in male sexual behavior were observed following chlorpromazine and chlordiazepoxide. All dose levels of oxazepam depressed male sexual behavior. The highest dose of chlordiazepoxide and oxazepam attenuated the onset of female sexual behavior, and all dose levels reduced postcopulatory lordosis durations. Amphetamine did not interrupt either male or female sexual behavior, and chlorpromazine disrupted male but not female behavior.

Animals↗

The accessory gland proteins in male Drosophila: structural, reproductive, and evolutionary aspects.

Recent results from biochemical and molecular genetic studies of the accessory gland proteins in male Drosophila are reviewed. The most prominent feature is the species-specific variability. However, the analysis of the sex peptide in D. melanogaster shows that there is a strong homology in the molecular structure to the closely related sibling species, and that divergence increases with increasing phylogenetic distance. For this reason the sex peptide, after being transferred to the female genital tract during copulation, reduces receptivity and increases oviposition only in virgin females belonging to the same species group and subgroup. Even though studies were hitherto limited to a small number of the secretory components, it is evident that the accessory gland proteins play a key role in reproductive success of the fruit fly by changing female sexual behavior, supporting sperm transfer, storage and displacement. Thus, genes encoding the accessory gland proteins are apparently under strong evolutionary selection.

Animals↗