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At least 1,369 records · Page 76Linked to original sources
PubMed2002

High throughput peptide mass fingerprinting and protein macroarray analysis using chemical printing strategies.

We describe a chemical printer that uses piezoelectric pulsing for rapid, accurate, and non-contact microdispensing of fluid for proteomic analysis of immobilized protein macroarrays. We demonstrate protein digestion and peptide mass fingerprinting analysis of human plasma and platelet proteins direct from a membrane surface subsequent to defined microdispensing of trypsin and matrix solutions, hence bypassing multiple liquid-handling steps. Detection of low abundance, alkaline proteins from whole human platelet extracts has been highlighted. Membrane immobilization of protein permits archiving of samples pre-/post-analysis and provides a means for subanalysis using multiple chemistries. This study highlights the ability to increase sequence coverage for protein identification using multiple enzymes and to characterize N-glycosylation modifications using a combination of PNGase F and trypsin. We also demonstrate microdispensing of multiple serum samples in a quantitative microenzyme-linked immunosorbent assay format to rapidly screen protein macroarrays for pathogen-derived antigens. We anticipate the chemical printer will be a major component of proteomic platforms for high throughput protein identification and characterization with widespread applications in biomedical and diagnostic discovery.

Amino Acid Sequence↗
PubMedDate not supplied

The social construction of the crack epidemic in the print media.

Early news coverage of the rapid expansions and horrors associated with use of crack in the mid-1980s led to a great panic. Scholarly research subsequently debunked the various myths emanating from this media scare. This article examines whether this expanded understanding was reflected in the quality of news coverage over time through a comprehensive examination of all articles about crack cocaine appearing in the New York Times, Time, and Newsweek from 1985 through 1995. It was found that later news stories were generally less blatant, but overall did not correct for previous exaggerations. The long-term perspective also revealed an insidious bias in news coverage through its focus on the inner city, in spite of broader use of crack. This misleading view of "the problem" constructed by the media probably helped divert attention from persistent structural problems facing the inner-city. Scholars and activists need to continue their efforts to focus attention on the underlying social problems to counteract the media's propensity to focus on what might more appropriately be termed symptoms, such as the "crack epidemic."

Cocaine-Related Disorders↗
PubMed1996

So you want to publish in JACH? How to avoid some potholes and pitfallls and make it into print.

Many college health professionals are intimidated at the thought of submitting an article to the Journal of American College Health. In this article, the editors provide details about the scope of the journal, describe types of articles it publishes, warn about common errors authors sometimes make in writing and submitting manuscripts for publication, and outline the steps in the review process.

Periodicals as Topic↗
PubMed2005

Media advocacy and newspaper coverage of tobacco issues: a comparative analysis of 1 year's print news in the United States and Australia.

Tobacco control advocates now recognize the value of influencing news coverage of tobacco; news coverage influences attitudes and behavior as well as policy progression. It is, however, difficult to assess the progress of such efforts within a single national and temporal context. Our data represent the first systematic international comparison of press coverage of tobacco issues. Tobacco articles from major daily newspapers in Australia (12 newspapers; 1,188 articles) and the United States (30 newspapers; 1,317 articles) were collected over 1 year (2001). The analysis shows that coverage in the two countries was similarly apportioned between hard news (>70%) and opinion pieces. Similarly, stories in both countries were most likely to recount positive events. The substantive focus of coverage, however, differed, as did the expression of hostile opinion toward tobacco control efforts (United States, 4%; Australia, 7.1%). Although secondhand smoke and education, cessation, and prevention efforts were covered widely in both settings, these topics dominated coverage in Australia (29.2%) more than in the United States (17.6%), where a more diffuse set of tobacco topics gained relative prominence. The difference in policy conditions seems to offer contrasting opportunities for advocates in the two countries to use newspapers to promote helpful tobacco control messages for both behavior and policy change.

Australia↗
PubMed2003

Communicating anthrax in 2001: a comparison of CDC information and print media accounts.

Information about anthrax released by news media from October 4 to December 3, 2001, was identified, sampled, coded, and compared with information released by CDC during that period using statistical analysis. In addition, communications about two anthrax-related issues were examined in depth. The quantitative analysis showed that, overall, CDC information releases and news coverage tracked fairly closely. When weight was defined as number of mentions, both sources gave the same weight to reports of risk for the population. The news sample gave roughly half the weight as CDC to who was exposed, how people were exposed, and what role antibiotics play in preventing anthrax. The samples were widely divergent (CDC high, news sample low) for public health precautions and other details. The in-depth, qualitative analysis showed that some reporters misinterpreted information provided by CDC, but they responded to requests to clarify the issue. The findings of this study suggest ways to improve future crisis communication efforts and demonstrate how differing methods of analysis can yield substantially different conclusions.

Anthrax↗