Fatty acid synthetase from lactating rat mammary gland. I. Isolation and properties.
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To study some changes that take place in the liver after schistosomal infection, the level of five vitamins of the B-complex group was estimated in non-infected mice and in 8, 16 and 20 weeks after the exposure of mice to cercariae of Schisostoma mansoni. Schistosomiasis caused a decrease in the level of thiamine, pantothenic acid, and niacin, but did not affect the concentration of riboflavin and pyridoxine in the liver.
Experiments were carried out to examine the growth and metabolism of thermotolerant yeast Candida tropicalis K-41 and bacteria Micrococcus freudenreichii that do not have a single temperature point but instead have an optimal temperature plateau at which the growth rate and biosynthetic activity remain unaltered or change insignificantly. Upon transition from the carbohydrate to the hydrocarbon pattern of nutrition these microorganisms show significant changes in metabolic processes: optimal concentration of biotin in the medium decreases significantly; the synthesis of riboflavin, nicotinic and pantothenic acids increases in yeast; the synthesis of nicotinic acid, biotin and vitamin B12 increases in bacteria. During microbial cultivation on hydrocarbons the content of cell lipids grows; yeast accumulate actively phospholipids and free fatty acids; bacteria build up intensively waxes and phospholipids. With the near-maximal growth rate the total synthesis of lipids decreases on carbohydrates and increases drastically on hydrocarbons, primarily at the expense of the above fractions.
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A growth study was conducted of rats continuously exposed for 4 weeks to ambient air, 1 ATA He-O2, or 11 ATA He-O2 conditions and fed one of 16 diets. The diets were the standard diet alone (adequate according to National Research Council standards); the standard diet with additional casein (50%), fat (25%), and all vitamins (25%); or the standard diet with all vitamins (25%), or all vitamins increased (25%) except one, which was supplied at the standard level. The standard diet was inadequate to support a normal rate of growth when fed under 11 ATA He-O2 conditions. Supplemental casein, fat, and vitamins or all vitamins alone adequately provided nutrients necessary for a normal rate of growth by hyperbaric exposed rats. The standard levels of thiamine, pantothenic acid, biotin, and vitamin K were inadequate and the standard levels of niacin, and vitamins A, D, and E were marginal in supporting growth when fed under hyperbaric conditions.
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The effect of the oral administration of a series of aliphatic alcohols (methanol to butanol) in addition to isopropanol on the liver contents of thiamine, riboflavin, pyridoxine, niacin, and pantothenic acid was investigated in rats. It was found the tested alcohols caused a significant decrease in the liver contents of the studied vitamins. This effect was observed after daily oral administration of the tested alcohols in doses of 1 and 2 mg/kg for 7 days. The diminishing effect of alcohols on the liver contents of vitamins was directly proportional to the dose administered.
Nine vitamin and mineral elements were determined in goat's milk of two breeds, Aardi and Masri, in Saudi Arabia. The concentrations of various vitamins in the milk of the Aardi and Masri goats, respectively, were: vitamin A, 77 and 203 IU/100 gm; thiamin, 0.047 and 0.030 mg/100 gm; riboflavin, 0.122 and 0.170 mg/100 gm; vitamin B-6, 0.045 and 0.046 mg/100 gm; niacin, 0.280 and 0.248 mg/100 gm; pantothenic acid, 0.247 and 0.191 mg/100 gm; ascorbic acid, 0.890 and 0.898 mg/100 gm; vitamin B-12, 0.07 and 0.06 microgram/100 gm; and folacin, 0.2 and 0.7 microgram/100 gm. Among the minerals, the concentrations (mg/100 gm) in the milk of Aardi and Masri goats, respectively, were: sodium, 58 and 53; potassium, 158 and 155; calcium, 88 and 116; magnesium, 13 and 13; phosphorus, 65 and 88; iron, 0.14 and 0.14; copper, 0.07 and 0.07; zinc, 0.15 and 0.15; and manganese, 0.02 and 0.02. The concentrations of vitamin B-12 and folacin in goat's milk were lower than those in cow's milk. The mineral profile of goat's milk compared better with that of cow's milk than with that of human milk.