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At least 1,369 records · Page 76Linked to original sources
PubMed1993

Effect of dietary sodium bicarbonate on growth, liver copper concentration and incidence of gastric ulceration in pigs fed excess dietary copper.

An experiment was conducted with growing-finishing pigs to evaluate the interactive effects of dietary sodium bicarbonate (1%) and excess dietary Cu (250 mg/kg diet) on growth, liver Cu accumulation and incidence of gastric ulceration. A 2 x 2 factorial arrangement of treatments was used and each treatment was replicated four (Trial 1) or three (Trial 2) times with four pigs per replicate. Initial weight of the pigs was 57 (Trial 1) and 32 kg (Trial 2). Growth rate and efficiency of feed utilization were not affected (P > .10) by treatment. Sodium bicarbonate increased (P < .06) the incidence of gastric ulceration and decreased (P < .01) dressing percentage. Dietary Cu (P < .01) increased liver Cu concentration but did not affect (P > .10) the incidence of gastric ulceration.

Animal Feed↗
PubMed1996

Accurate measurement of copper-67 in the presence of copper-64 contaminant using a dose calibrator.

UNLABELLED: The use of 67Cu-labeled antibodies for the treatment of cancer has advanced to the clinical trial phase. Quantitation of 67Cu radiopharmaceuticals is complicated by the presence of the radioimpurity of 64Cu in 67Cu supplies. Here we report a method to assay 67Cu and 64Cu in a mixed sample with a commonly available instrument, the ionization chamber dose calibrator. METHODS: The activities of 67Cu and 64Cu in a mixed sample can be calculated from a single-dose calibrator measurement. The calculation requires (1) instrument-specific response coefficients D67 and D64, generated by gauging the instrument for the efficiency of measurement of 67Cu and 64Cu, and (2) a value for the ratio of 67Cu to 64Cu in the sample, routinely provided by major suppliers of 67Cu. D67 and D64 were empirically determined by measuring samples containing known amounts of 67Cu and 64Cu. The samples were also assayed by gamma ray spectroscopy to verify the isotope ratios given by the suppliers. RESULTS: This method generated accurate response coefficients. At the recommended dose calibrator setting for the measurement of 67Cu, at which D67 = 1.0, the measurement for D67 with this method was 1.02 (+/- 0.04). Isotope ratios provided by the radionuclide suppliers were corroborated by gamma ray spectroscopy. CONCLUSION: A method is presented by which 67Cu and 64Cu in a mixed sample can be assayed using a dose calibrator. Although the derived numeric constants are only correct for a specific dose calibrator and setting, the method can be adapted for use with any dose calibrator.

Calibration↗
PubMed1996

Low molecular weight copper-binding protein in the liver of rats given a single and repeated injections of copper.

Whether Cu-metallothionein is the sole low molecular weight Cu-binding protein induced in Cu-loaded rat tissues or not is hotly debated, because several investigators reported that Cu-metallothionein did not exist and other Cu-binding proteins were induced in the Cu-loaded rat liver. Investigation by using rats given a single and repeated injections of Cu led us to settle the problem. Cu-metallothionein was identified in the liver of both groups of rats by the purification procedure established in this study. Moreover in the final purification step of Cu-metallothionein, an additional unknown protein which did not belong to the metallothionein group was found only in the rats given repeated Cu injections, and not in the rats given a single Cu injection. The confusion on Cu-metallothionein and other Cu-binding proteins was shown to be caused by inadequate purification of Cu-metallothionein.

Amino Acid Sequence↗