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At least 1,369 records · Page 76Linked to original sources
PubMed1999

[The clinical characters of tuberculous otitis media].

OBJECTIVE: To sum up the clinical characters of tuberculous otitis media. METHOD: 2 cases of tuberculous otitis media and other reports are reviewed. RESULT: The onset of this disease is sudden and rapid with little discharge coming from the ear. Sometimes one or more perforation and granulation tissue can be found. Necrotizing bone may be seen by radiography. CONCLUSION: According to the tuberculous history of patients or their families and the clinical features, the diagnosis and the treatment to this disease are not difficult.

Adult↗
PubMed2001

[Influences of the mobile phase constitution, salt concentration and pH value on retention characters of proteins on the metal chelate column].

The effects of the nature and concentration of salts, pH value and competitive eluent in the mobile phase on the protein retention have been systematically investigated. A mathematical expression describing the protein retention in metal chelate chromatography has been derived. It is proposed that the eluting power of the salt solution can be expressed by the eluent strength exponent epsilon. According to the retention characters of protein under different chromatographic conditions, the interaction between the various metal chelate ligands and proteins is discussed. The protein retention on the metal chelate column is a cooperative interactions of coordination, electrostatic and hydrophobic interaction. For the strong combined metal column with proteins such as IDA-Cu, the coordination is the most important, and the electrostatic interaction is secondary in chromatographic process. However, for the weak combined metal columns with proteins such as IDA-Ni, IDA-Co and IDA-Zn, the electrostatic interaction between the metal chelate ligands and proteins is the chief one, while the coordination is the next in importance. When the mobile phase contains high concentration of salt which can't form complex with the immobilized metal, the hydrophobic interaction between the protein and stationary phase will be increased. As the interaction between the metal chelate ligand and proteins relates to chromatographic operating conditions closely, different elution processes may be selected for different metal chelate columns. The gradient elution is generally performed by the low concentration of salt or different pH for weakly combined columns with proteins, however the competitive elution procedure is commonly utilized for strongly combined column. The experiment showed that NH3 is an excellent competitive eluent. It isn't only give the efficient separation of proteins, but also has the advantages of cheapness, less bleeding of the immobilized metals and ease of controlling NH3 concentration. The interaction between the metal chelate ligand and proteins and the selectivity of metal chelate chromatography can be changed through changing chromatographic conditions.

Chelating Agents↗
PubMed1999

[The clinical characters of myopatic pharyngoparalysis and laryngoparalysis with 3 cases report].

OBJECTIVE: To improve the level of diagnosis and treatment about myopathic pharyngoparalysis and laryngoparalysis (MPL). METHOD: 3 cases of MPL were diagnosed by clinical inaterials, electromyogram, serokinase and muscle biopsy. The clinical characters are dysphagia, phonasthenia, abnormal electromyogram and high CPK et al. They were all treated with glucocorticoid. RESULT: The effect of treatment on all 3 cases was good. CONCLUSION: It is important to distinguish MPL with neuropathic pharyngoparalysis and laryngoparalysis. An appropriate glucocorticoid treatment is also impontant.

Adolescent↗
PubMed2001

[Character of alpha-crystallin molecular chaperone activity in selenite cataract].

PURPOSE: To evaluate the character of alpha-crystallin molecular chaperone activity in selenite cataract. METHODS: alpha-Crystallin from lenses of normal young rats and lenses with selenite cataract were separated and purified by gel filtration chromatography. The chaperone activity of alpha-crystallin was assayed by mearsuring heat-induced aggregation and scattering of catalase. RESULTS: alpha-Crystallin in both normal and cataractous lenses specifically prevented catalase against thermal aggregation compared with control proteins. The preventive ability in alpha H-crystallin was less than in alpha L-crystallin. Chaperone activity was diminished in selenite cataract. There was statistically significant difference in chaperone activity between normal and cataractous lenses in both alpha H-crystallin and alpha L-crystallin respectively. CONCLUSION: The reduction of alpha-crystallin chaperone activity in selenite cataract by preventing thermal aggregation of catalase is an important process in cataract formation.

Animals↗
PubMed1999

[Character identification of 12 kinds of pilose antler medicinal materials].

In this paper, commercial medicinal materials of 12 kinds of pilose antler, Cervus nippon, C. elaphus, C. albirostris, C. unicolor, C. eldihainanus, C. timorensis C. porinus, Dama dama, Rangifer farandus, Alces alces, Elaphurus davidianus, Capreolus capreolus were compared and identified. A key and simple character illustration were listed.

Animals↗
PubMed2002

[Unique ecosystem characters and ecological principles for development in the ecotones between agriculture and pasture in north China].

Based on the fact that its ecological deterioration, a rethinking on the former definition of 'transitional zone between agriculture and grassland' was proposed. It was recognized that this zone has been gradually and dynamically formed under the specific circumstances of history and anthroponomy in China. It should thus be re-defined as 'ecotone between agriculture and pasture', instead of just focusing on its features of geography, ecology, and vegetation separately, as well as its character of transition. More attention should be put on its nature of complex system of ecology, society and economy, and then, derive relevant abating measures. Boreal ecotone has the functions of life support for a relatively dense population, as well as ecosystem services. Under the premise of unable copying the models for arid and semiarid areas in abroad, in particular developed countries, an unique strategy of capable of compromising both above-mentioned functions, as well as ecological reconstruction, which is embodied in readjusting agro-structure, rationally utilizing and conserving resources, and establishing 'polar' industries, such as grasses and livestock, should be the center of an interdisciplinary study approach.

Agriculture↗
PubMed2003

[Infection characters of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in immunodeficient guinea pigs].

OBJECTIVE: The aim of this study was to investigate effects of immunodeficiency on the periodontal infection characters of the specific pathogens of juvenile periodontitis. METHODS: A total of 36 immunodeficient guinea pigs produced by twice whole-body irradiation with 60Co were divided randomly into four groups, in which Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and A. actinomycetemcomitans with P. gingivalis were inoculated into the gingival sulcus of two mandibular incisors respectively. The pigs in the control group did not receive any inoculation. At 2, 3 and 6 weeks after inoculation, three animals in each group were sacrificed successively. Clinical and histological examinations were used to examine the changes in the periodontal tissues. The other 36 normal guinea pigs were divided into four groups and treated in a similar way described above. RESULTS: Significant periodontal damages were noted in immunodeficient pigs inoculated with A. actinomycetemcomitans, P. gingivalis or A. actinomycetemcomitans and P. gingivalis in 2 and 3 weeks after bacterial inoculation. The damages were more severe than in the normal groups. The immunodeficient groups demonstrated larger numbers of osteoclasts than the normal groups (P < 0.05). CONCLUSION: The loss of periodontal tissue in immunodeficient hosts is much serious than those with normal defence system, after they are infected with A. actinomycetemcomitans and P. gingivalis. Abnormal defence system in hosts may play an important role in onset and development of juvenile periodontitis.

Actinobacillus Infections↗
PubMed2003

[Clinical characters, diagnosis and treatment of parapharyngeal region neoplasms: a report of 115 cases].

OBJECTIVE: The aim of this study was to analyze the clinical characters, diagnosis and treatment of primary neoplasms in the parapharyngeal regions. METHODS: A total of 115 cases of primary neoplasms in the parapharyngeal regions were included in this study. RESULTS: Disorders of swallowing, breath or speaking were the common symptoms of neoplasms in parapharyngeal regions. The majority of neoplasms parapharyngeal regions were benign. 46 cases were from the deep lobe of the parotid, and 42 cases were neurogenic. CT or MRI provides useful information such as their sizes, shapes and extents. The relationship between the tumor and vessel was shown using super-selective angiography. All the tumors were resected completely by extra-oral approach through the posteroinferior incision of the mandible. The follow-up was carried out from 1 to 10 years, and the data indicated that 2 patients died, 18 cases were lost, and others had no recurrence. CONCLUSION: CT and/or MRI are essentially useful for diagnosis and presurgical planning. Super-selective angiography can be used if the lesions involved carotid artery. Posteroinferior incision of the mandible is a simple, effective and safe approach for excision of neoplasms parapharyngeal regions.

Adolescent↗
PubMed1999

Character of Immune Disturbances and Their Correction by Including the Interferon Inductor in Complex Therapy for Turboperitoneal Infertility Associated with Chlamydial Infection.

The character of immune disturbances was studied in women with tuboperitoneal infertility associated with a chlamydial infection. The immunological methods included a study of the population and subpopulation composition of peripheral blood lymphocytes (CD3, CD7, CD22, CD4, CD8, ratio CD4/CD8). The general hemolytic activity of the complement and main components of the complement (C1-C5), the level of IgA, IgM, IgG, the concentration of CIC, the content of IL-2 and its soluble receptor (sIL-2R) were determined in blood serum and in peritoneal fluid before and after treatment. A reduction in the absolute content of lymphocytes, T helpers, the number of B lymphocytes and their functions, the absence of activation of the T cell compartment, is detected. Evidence of this is provided by the low level of sIL-2R and the absence of a reliable rise of IL-2 in the blood serum and peritoneal fluid. The complex therapy of tuboperitoneal infertility associated with a chlamydial infection, with the obligatory inclusion of immunomodulators was effective and was accompanied by normalization of the majority of the immunity indices. Use of the interferon inductor ensured the maximum increase of the absolute content of the lymphocytes in circulation, without any pyrogenic reaction. This made interferon inductors preferable for therapeutic use in the case of this pathology.

Journal Article↗
PubMedDate not supplied

[Character and circadian patterns of disturbances of the heart's rhythm in patients with postinfarction cardiosclerosis and pre- and early postinfarction angina (with data from Holter electrocardiogram monitoring)].

An evaluation was done of the character and circadian patterns of cardiac rhythm in 157 patients with ischemic heart disease, postinfarction cardiosclerosis, who ranged from 34 to 73 years old, with a history of preinfarction and early postinfarction stenocardia. It has been found out that in patients with postinfarction cardiosclerosis there prevail supraventricular and ventricular extrasistole in the structure of the heart's rhythm disturbances. Other rhythm disturbances occur very seldom. Supraventricular and ventricular tachycardia are more commonly seen in patients with a history of early postinfarction angina. The above patients demonstrate an increase in both the number of extrasystoles and in their gradation suggesting to us an electrical instability of the myocardium. Ventricular extrasystole was seen significantly less frequently in those patients presenting with preinfarction stenocardia, which fact might be related to a protective action of the ischemic preconditioning phenomenon.

Adult↗
PubMed2003

[Isolation, culture and biological character studies on human fetal pancreatic nestin positive cells].

Pancreatic nestin positive cells are multipotential stem cells which may play an important role in the development of pancreas. Here we isolated nestin positive cells from human fetal pancreases and then examined its biological characters in vitro. The results showed that: (1) Nestin positive cells expressed high level transcript of ABCG2/BCRP1 which was the molecular determination of stem cells, and they differed from pancreatic ductal epithelium cells both in morphology and in growth behavior. (2) Upon confluence, these cells self assemble to form islet like cell clusters (ICCs). (3) During differentiation, nestin positive cells in ICCs expressed markers of different cell lineages which meant that they were multipotential, and they could generate insulin-producing cells after inducement.

Cell Differentiation↗
PubMed2003

[Effect of ribozyme against platelet-derived growth factor receptor beta subunit mRNA on the biological characters of hepatic stellate cells].

OBJECTIVE: To study the cleavage activity of hammerhead ribozyme targeting at platelet-derived growth factor receptor beta subunit (PDGFR- beta) mRNA in hepatic stellate cells (HSCs) and its effect on the biological characters of HSCs. METHODS: Expression vector of anti-PDGFR- beta ribozyme was constructed and transfected into rat-derived HSC-T6 cells with lipofectin. The positive cell clones were gained by G418 selection. The expression of PDGFR- beta, alpha-smooth muscle actin (alpha-SMA), and type I and type III collagen was detected by means of northern blot, Western blot and immunocytochemical staining respectively. The cell proliferation was determined with MTT colorimetric assay. The cell apoptosis was demonstrated with flow cytometry, acridine orange fluorescence vital staining and transmission electron microscopy. RESULTS: The expression of PDGFR- beta at mRNA and protein level was markedly reduced in ribozyme-transfected HSCs only 43% to 51% of that in control cells (t > or = 3.957, P < 0.05), and alpha-SMA expression level, type I and type III collagen synthesis ability were also reduced (t > or = 6.790, P < 0.01). The proliferation of ribozyme-transfected HSCs was significantly decreased (t > or = 3.858, P < 0.05), and the proliferation response to PDGF BB was markedly inhibited. However the apoptotic rate was significantly increased in ribozyme-transfected HSCs (chi2 > or = 14.157, P < 0.01), and typical apoptotic cells could be found under transmission electron microscopy. CONCLUSIONS: The anti-PDGFR- beta ribozyme can be expressed stably in HSCs, cleave the target RNA effectively, inhibit HSCs proliferation and collagen synthesis, and induce HSC apoptosis. The results suggest that inhibiting PDGFR- beta expression in HSCs may be a new therapy for liver fibrosis.

Apoptosis↗
PubMed2003

Morphological characters of adult Anopheles (Nyssorhynchus) marajoara in Venezuela.

A morphometric study was carried out to find diagnostic characters with which to update taxonomic keys for field identification of Anopheles (Nyssorhynchus) marajoara and the 3 other sympatric Anopheles (Nyssorhynchus) species (An. darlingi, An. argyritarsis, and An. braziliensis) that occur in Venezuela. Diagnostic random amplified polymorphic DNA-polymerase chain reaction markers from wild-caught specimens showed that An. marajoara was the only species in the Anopheles albitarsis complex collected in Venezuela.

Animals↗
PubMed2003

[Life-table of Helicoverpa armigera in northern China and characters of population development in southern and northern China].

The population dynamics of Helicoverpa armigera in Raoyang County, Hebei Province as an example in Northern China was researched, and its natural life-table was established. The results showed that total mortality of first to fourth generations of cotton bollworm in Northern China were 98.3%, 98.34%, 98.69% and 99.13%, and the population tendency index (I) were 3.97, 1.50, 1.41 and 1.09, respectively. A comparative analysis of the natural life-table of cotton bollworm in Southern China and in Northern China showed that the survival curves were concave curves in the two cotton zones. The curve showed that the mortality of cotton bollworm was higher in its egg stage to the second instar, and lower after the third instar. The key death factor of cotton bollworm was the scouring effect of rain and wind in Southern China, and the predation of its natural enemies in Northern China. The hatch period to the first instar was the key stage in Northern China and Southern China, and in this time, its larva instar was slim, fragile and sensitive to bad circumstances, so its mortality was higher. The second and third generations were the key damage generations in Northern China, while the third and fourth generations were the key damage generations in Southern China. The development and damage characters of each generation in Northern China and in Southern China were discussed.

Animals↗
PubMed2001

[Purification of recombinant hEGF expressed in yeast Pichia pastoris and the study on its characters].

OBJECTIVE: To obtain recombinant human epidermal growth factor(hEGF) that can be used in animal experiments and clinical trial. METHOD: Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow(high sub), Q-sepharose High Performance, and Superdex 30 chromatography, and its characters were studied by respective methods. RESULTS: The purified hEGF doesn't contain pyrogen, endotoxin, or yeast chromosome DNA and the purity reached 98%. The recombinant human EGF has correct molecular weight, pI, N-terminal amino acids sequences, peptide map, ultraviolet spectrum and well-biological activity. CONCLUSION: The purified hEGF is in accord with the requirements for animal experiments and clinical trial which provides the basis of preparing EGF agents for clinical test.

Animals↗