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At least 1,333 records · Page 74Linked to original sources
PubMed1991

[Reducing the duration of revoked driving permit penalty in alcoholic drivers following psychological remedial traffic education].

1) Also new facts--driver improvement, longstanding good reputation in road traffic (minimum 25 years)--are only conditionally acceptable to substantiate a reduction in the blocking period in terms of section 69 a section 7 StGB. 2) A high blood alcohol level (as from 0.2 g %) precludes the reduction in the blocking period for obtaining a driving licence of drunken drivers. 3) Attending a post-schooling course may, in individual cases, as a new fact able to justify a reduction of blocking period. 4) In many cases the court is insisting behind own behaviour prognosis on a specialist for psychological selection tests.

Accidents, Traffic↗
PubMed1991

[Which evidence permits a liver cell smear in cattle with metabolic disorders?].

The technique of liver puncture as described by Holtenius (1961) was assessed with regard to its practicability and safety in a preliminary study of 26 cattle and a follow-up study of 108 German black pied cattle. The sample material was smeared, stained and examined by light microscope. All animals with changes of grade 5 had to be slaughtered. Liver puncture is a simple, quick and safe technique which supplies useful information about the various stages of hepatic fatty degeneration in individual cattle.

Animals↗
PubMed1991

[Difference reflectometry might permit process control for dental laser applications].

Difference reflectometry is a new method for assessing material surfaces by measuring the relative amount of light reflected within a range of 300-720 nm. The in vivo and in vitro results of the present study indicate that it is possible to transfer this measuring principle to dental applications. Reflexion measurements allow the detection of hard tooth structures and their pathologic alterations with adequate exactness. With the aid of this method it is possible to differentiate between carious hard tissues and clinically healthy tissues by color and surface structure during excavation. It also allows a reliable demarcation from oral soft tissues. Due to its high measuring frequency it may be used as on-line process control. In contrast to other spectrometric methods it requires a minimum of equipment and is easy to handle.

Dental Caries↗
PubMed1991

Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template.

A simple, rapid method is presented for the enzymatic sequence analysis of nanogram amounts of single-stranded or double-stranded DNA. This approach employs the thermostable DNA polymerase from Bacillus sterothermophilus and exploits its ability to efficiently extend all of the template-primer complex, even at low substrate concentrations. The procedure requires few pipetting steps, no preannealing step and very short reaction time. This method can significantly reduce the cost associated with DNA polymerase and the amount of template and time required to perform the enzymatic sequencing reactions. As little as a 10-ng aliquot of such sequencing reactions can be analyzed on a fluorescence-based capillary gel electrophoresis instrument recently developed in our laboratory. This highly sensitive detection, in conjunction with the ability to efficiently sequence nanogram amounts of template, strongly suggests the feasibility of direct DNA sequencing of single bacteriophage M13 plaques without prior amplification.

Bacteriophages↗
PubMed1991

Lipid hydroperoxides permit deformation-dependent leak of monovalent cation from erythrocytes.

Subtle peroxidative perturbation of normal red blood cells (RBC) using t-butylhydroperoxide creates a leak pathway for monovalent cations that is reversibly activated by cell deformation. To determine what factor promotes expression of this unique membrane defect, we have dissected "peroxidation" into components that can be evaluated separately by comparing K leak from suitably modified RBC during elliptical deformation and parallel control incubation. Selective introduction of phospholipid hydroperoxides into normal RBC membranes successfully induces a deformation-dependent leak pathway having the same phenomenology as that previously documented for cells treated with t-butylhydroperoxide itself (fully recoverable; calcium-independent; inhibited at lower pH; K efflux balanced by Na influx). This leak pathway occurs in the absence of detectable secondary peroxidative change and appears to reflect a direct influence of lipid hydroperoxide. Using micropipette examination of vesicular bilayers reconstituted from RBC lipid extracts, we find that lipid from peroxidized RBC exhibits only a slight tendency to be less cohesive than normal lipid, apparently precluding isolated lipid properties as an explanation for altered permeability barrier function. However, addition of a hydrophobic membrane-spanning peptide to these same lipids significantly diminishes bilayer cohesion, an effect that is exacerbated further by the presence of peroxidized lipid. These observations suggest that lipid hydroperoxide is a necessary, but perhaps not sufficient, factor for induction of this unique leak pathway. Our results may be relevant to the abnormal cation homeostasis of sickle RBC in which deformation of an oxidatively perturbed membrane occurs during the sickling phenomenon.

Biophysical Phenomena↗
PubMed1990

[Do clinical parameters exist that permit predicting the need to combine a diuretic with captopril in the initial treatment of essential arterial hypertension?].

In the present work the clinical, biological, radiological, electrocardiographic and hormonal characteristics are analyzed in 51 patients suffering mild essential hypertension, in whom treatment with captopril in monotherapy or associated to chlortalidone managed to normalize arterial pressure and maintained the pressure control during a period of one year. Captopril in monotherapy at a dose of 50 to 150 mg/day normalized blood pressure in 34 patients (66.7%) while in the remaining 17 patients (33.3%) the association of 25 mg of Chlortalidone was required. When comparing the subgroup of patients whose blood pressure levels were controlled with captopril as the only used drug (Group A) against those who required the association with diuretics (Group B), we could only observe significant differences regarding the blood pressure level and cardiothoracic index, being these higher in the group of patients who required pharmacologic association. We conclude that only the severity of hypertension allows to predict the necessity of associating a diuretic to captopril in order to obtain the control of blood pressure levels.

Adult↗
PubMedDate not supplied

Paraformaldehyde fixation of LPS-stimulated human monocytes: technical parameters permitting the study of membrane IL-1 activity.

The existence of IL-1 activity on the cell surface of stimulated mononuclear phagocytes is a matter of controversy. In particular, fixation of IL-1-expressing cells for 15 min in 1% paraformaldehyde (PFA) is commonly used to evidence such "membrane-associated" IL-1 activity but other authors have attributed this to passive leakage of IL-1 alpha from the cells and report no activity with longer fixation times. Using specific IL-1 alpha and IL-1 beta assays, we found that after the mild standard PFA fixation procedure, not only IL-1 alpha but also IL-1 beta were released into the supernatants for up to 96 h following fixation; membrane IL-1 activity cannot thus be measured in these conditions. However, using conditions in which neither immunoreactive IL-1 molecules nor IL-1 activity are found in the supernatants (i.e. assay at 144 h, increased fixation time), we were still able to detect IL-1 activity on LPS-stimulated, PFA-fixed monocytes. This activity was independent of the duration of PFA fixation and was inhibited by anti-IL-1 alpha but not anti-IL-1 beta antibodies. Our data thus underline the importance of technical conditions in the study of membrane-associated IL-1 activity.

Adult↗