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At least 1,333 records · Page 74Linked to original sources
PubMed1996

[Expression of cDNA of human chorionic gonadotropin beta-subunit (beta-hCG) cDNA in insect cells and effect of expressed product on mouse lymphocytes in vitro].

Expression vector pVL 1393-hCG beta containing beta-hCG cDNA has been constructed using an unfused protein nuclear polyhedrosis virus (AcNPV) expression vector. The insect cells (Sf 9) were cotransfected by the expression vector and nuclear polyhedrosis virus genomic DNA, and recombinant virus AcNPV-hCG beta was screened out, beta-hCG cDNA was expressed in insect cells infected by recombinant virus and recombinant beta-hCG (r beta-hCG) was secreted into medium. The purity of r beta-hCG, purified by immuno-affinity chromatography, was about 90% and the molecular weight of r beta-hCG was 22,500 Da. Like hCG, r beta-hCG suppressed significantly proliferation of induced lymphocytes, as well as production of IL-2 to some extent, on a parallel with suppression of lymphoproliferation.

Animals↗
PubMed1964

TICK-BORNE ENCEPHALITIS IN THE USSR.

The author gives the history of the study of tick-borne encephalitis in the USSR, following it up with a description of the etiological and epidemiological aspects and of the pathology, symptomology, diagnosis, treatment, and prevention of the disease. He stresses that other ticks as well as the main vectors of the virus, Ixodes, transmit the disease, in particular Dermacentor and Haemaphysalis. A detailed account of tick-borne encephalitis in the USSR may be of value in the study of the rather similar forms of the disease now being encountered more and more frequently in countries of South-East Asia as well as of northern and eastern Europe.

Allergy and Immunology↗
PubMed1962

A survey of Simulium control in Africa.

It has become possible to control or even eradicate the Simulium fly vectors of Onchocerca volvulus, the causative organism of onchocerciasis. There are two vectors in Africa-namely, S. damnosum, characteristic of the rivers of West Africa, and S. neavei, which breeds on the carapaces of crabs in the streams of East Africa. The use of DDT applied to the water at a concentration as low as 0.1 p.p.m. for 30 minutes eliminates the larvae of Simulium. Such larvicidal methods have eradicated S. neavei from western Kenya and virtually eradicated S. damnosum from the Victoria Nile in Uganda. Excellent control sufficient to render the transmission of onchocerciasis almost negligible has been obtained at Léopoldville (Republic of the Congo) and in circumscribed areas in southern Chad, Northern Nigeria, and Sierra Leone. The following survey describes operational research on Simulium control carried out in Kenya, Uganda, the Congo, Chad, Nigeria, Ghana, Upper Volta and Sierra Leone.

Africa↗
PubMed1993

Conformation dependence of antipeptide antibodies: characterization of cell-CAM105 isoform-specific antipeptide antibodies using proteins expressed in insect cells with baculoviral vectors.

Cell-CAM105 proteins are hepatocyte adhesion molecules of the immunoglobulin superfamily. The two isoforms, L-form and S-form, are highly homologous. In their extracellular domains, only 16 amino acid substitutions are found scattered in the first immunoglobulin domain of 105 amino acids. Peptide sequences containing these differences are selected for production of antibodies. Isoform specificities of these antibodies were evaluated with proteins expressed in the baculovirus-insect cell system. In immunoblot and immunoprecipitation experiments, anti-C1 antibody, which was generated against a peptide sequence found only in the cytoplasmic domain of the L-form, reacted only with the L-form cell-CAM105 protein. Anti-N antibody, which was generated against a pentadecapeptide of the L-form, reacted with both the S- and L-isoforms. The lack of isoform specificity of anti-N is not surprising because there is only one amino acid substitution in this pentadecapeptide sequence. In contrast, S3, a S-form-specific pentadecapeptide with four amino acid substitutions was able to elicit antibody, anti-S3, specific for the S-isoform. With the commonly used immunoprecipitation procedures, only anti-C1 was able to precipitate cell-CAM105 from the liver membrane. Anti-N and anti-S3 could precipitate the proteins only after the liver membrane samples had been boiled in the presence of denaturing agents. Hydropathy analysis of these peptides revealed that both peptides N and S3 are more hydrophobic than peptide C1, suggesting that the peptide fragments N and S3 are probably not located on the surface of the protein. This may explain why boiling of the protein sample was necessary before anti-N and anti-S3 could precipitate the protein. The present study demonstrates that it is possible to produce isoform-specific antibodies for highly homologous proteins. Furthermore, we show that special sample treatment may be required to expose the antigenic sites.

Adenosine Triphosphatases↗