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PubMed1983

[Therapeutic use of synthetic Gn-RH-Dirigestran in disorders of the reproductive cycle in cows from low-fertility herds].

Synthetic Gn-RH-Dirigestran Spofa was used for the treatment of 121 cows with functional ovarial disorders. Out of this total number, 41 cows showed no postpartal and no post-service symptoms of oestrus, 59 returned to oestrus with irregular cycles, and 21 cows showed an irregular course of oestrus. The treatment was made in herds with a low conception rate (32-37% conceptions after the first insemination, service period 108 to 142 days). On the whole, 80.1% of the treated cows got in calf, 53.7% conceived after the first insemination. The average time from treatment to successful insemination was 39 days. The treatment was started 148 days from parturition, on an average. The average insemination index of the treated cows was 1.44. As to cows out of heat, 70.7% got in calf on the whole and 51.2% after the first insemination, the therapeutic service period (TSP) being 44 days. Out of the cows returning to oestrus with an irregular cycle, 84.7% got in calf and 52.5% got in calf after the first insemination with the TSP of 37 days, and the cows with irregular cycle the conception was recorded in 85.7% (61.9% after the first insemination with the average TSP of 35 days). On the whole, 82% of treated cows showed clearly visible symptoms of oestrus after the application of Dirigestran. The pregnancy of the whole set (121 treated cows) was higher by 15 to 21% after the first insemination and by 41 to 49% after all inseminations, as compared with the pregnancy of normally inseminated cows in these herds. The results show that the treatment of the functional disorders of ovaries is also practicable in cows in herds with a decreased fertility if this treatment is not impaired by spermiotoxicity or embryotoxicity owing to the effects of inadequate secretions of sexual organs caused by incorrect nutrition or infections.

Anestrus↗
PubMed1984

[Submucosal vestibulo-vaginal administration of cloprostenol (Oestrophan-Spofa) in cows in relation to estrus activity, conception, and the level of progesterone in milk].

Trials were performed to examine the effectiveness of 250 micrograms (1 ml) of cloprostenol (Oestrophan Spofa) implantation under the mucous membrane of the vaginal vestibule of 128 cows with a clinically pronounced corpus luteum on ovaries. Within 72 hours from administration, oestrus was observed in 112 animals (85.5%). Out of the 97 cows inseminated, 63 cows (64.94%) got in calf. The effectiveness of the luteolytic action was examined on the basis of progesterone check in milk in 56 treated cows. The submucous implantation of cloprostenol rapidly degraded the function of the corpus luteum since from the original level of 14.78 ng/ml progesterone decreased to 0.87 ng/ml within 72 hours. However, luteolysis did not affect all the corpora lutea. Hence the submucous administration of cloprostenol was found to be effective, and at the same time, highly economical, owing to a substantial reduction in the costs of reproduction control as well as the costs of production.

Animals↗
PubMed1984

Embryo transfer in swine.

Embryo transfer is used to propagate genetically superior swine while minimizing disease risks. Embryos are harvested through a ventral midline incision in the anesthetized sow 4-6 days after estrus, using a glass tube or Foley catheter. Embryos are transferred to similarly prepared sows by injection directly into the uterus through a tomcat catheter or glass pipette. Embryos typically are transferred within a few hours of collection but may be stored in culture medium at 37 C for up to 30 hours.

Animals↗
PubMed1981

Endocrine and ovarian changes in dairy cattle fed a low beta-carotene diet during an oestrus synchronisation regime.

Twelve Friesian cows, 76 to 110 days calved, with blood cell counts and Compton metabolic profile values in the normal range throughout, were housed and fed a marginal diet for varying periods before being injected twice, 11 days apart, with cloprostenol. Artificial insemination was carried out 72 and 96 hours after the second injection. Plasma concentrations of progesterone, oestradiol 17 beta, luteinising hormone, follicle stimulating hormone, and beta-carotene were monitored during this regime as were uterine and ovarian changes. Progesterone profiles were followed for a further 21 days by assay of milk samples. Subsequent rectal examinations showed that five of the 12 cows conceived at controlled oestrus and another four within 52 days of this time. No correlation was observed between time of conception and condition score, metabolic profiles or haematological parameters; there was a correlation between time of conception and plasma beta-carotene concentrations and cows with lower beta-carotene values showed cyclic irregularities or appeared to have depressed steroid hormone production.

Animals↗