Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Electric Capacitance”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,333 records · Page 74Linked to original sources

Stimulus artefact reduction systems for the TDH-49 headphone in the recording of auditory evoked potentials.

Standard audiometric transducers, such as the TDH-49, produce an electrical artefact which interferes with the recording of the early auditory evoked potentials. The sources of these artefacts have been identified as the capacitive and inductive coupling of the transducer and its leads to the recording electrodes. Artefacts were recorded using an artificial head with an M2-Cz electrode array for 500- and 3-kHz tone pips and positive, negative, and alternating polarity clicks at Peak Equivalent Sound Pressure Levels from 60 dB (positive click and 3-kHz tone pip) to 90 dB (alternating polarity click). The artefacts grew linearly with increases in intensity. Shifts in the Visual Detection Threshold of the artefacts produced by various conditions of shielding the transducer and its leads were explored. Acceptable reductions in artefact magnitude were obtained by enclosing the transducer in either an aluminium or mu-metal case connected through a braided-wire shield of the transducer leads to earth ground. Lesser reductions were achieved by the use of antiphase cancellation. The combination of the latter and the aluminum and mu-metal cases did not produce greater shifts in the visual detection threshold than the use of the aluminum and mu-metal cases alone. The fabrication of a pair of shielded enclosures is described and data are presented which show that they do not adversely affect the acoustic output of the headphones.

Acoustics↗

Low-temperature resistance anomaly at SrTiO3 grain boundaries: evidence for an interface-induced phase transition.

Variable temperature transport between 1.4 and 300 K, structural imaging, and theoretical calculations were used to characterize the properties of electrically active 24 degrees and 36.8 degrees [001] tilt SrTiO3 grain boundaries with 0.1 at. % niobium doping. An anomaly in boundary resistance and capacitance characteristics typical of a positive temperature coefficient effect is observed. This behavior is indicative of interface-induced dipole ordering. The detailed atomic structures of these grain boundaries were determined from a comparison of ab initio calculations and Z-contrast TEM images. The number of excess electrons at the boundaries determined experimentally and theoretically agrees and is associated with the boundary structural units.

Journal Article↗

Changes in the properties and composition of zona pellucida of pigs during fertilization in vitro.

Several hundred fertilized pig eggs were prepared by an in vitro fertilization (IVF) technique in which follicular phase ovarian eggs were matured in vitro to metaphase II before incubation with capacitated epididymal spermatozoa for 12 h at 39 degrees C. Parthenogenetic eggs were also prepared by stimulation of the mature eggs with an electric pulse. The zonae were solubilized with 0.2% pronase/phosphate-buffered saline (PBS) or lactic acid/PBS. The time taken for solubilization was 30-40% shorter than for unfertilized eggs, indicating that zona hardening was induced during fertilization. At the same time, the sperm receptor activity of the zona was reduced. Electrophoretic analyses of zona glycoproteins from the ovarian, mature and fertilized eggs revealed that the amount of 90 kDa proteins decreased substantially during fertilization. This fraction could barely be detected in the zonae from parthenogenetic eggs. However, modification with a fluorescent probe showed that the general architecture of the zona remained unchanged during fertilization. These results suggest that the minor 90 kDa proteins are specifically degraded by the protease(s) released from the oocyte at fertilization, thereby leading to the block to polyspermy.

Animals↗

Theoretical analysis of a rectifying gap junction model.

Based on the experimental observations, an electrical circuitry model for a rectifying gap junction was developed in previous work. In order to show the physiological function for each element in the model, a theoretical analysis is carried out in this report. The results indicate that the gap junctional capacitance Cj newly introduced into the model plays a major role in determining the duration increase, the peak attenuation, and the peak delay on the postmembrane action potential. Also, a comparison between the newly proposed model and the widely accepted kinetic model by Giaume is made. The results show that the electrical circuitry model can not only describe the characteristics of Giaume's model but also allow the interpretation of some experimental phenomena that were not reflected in other gap junctional models.

Animals↗

Dielectric properties of yeast cells.

Dielectric measurements were made on suspensions of intact yeast cells over a frequency range of 10kHz to 100 MHz. The suspensions showed typical dielectric dispersions, which are considered to be caused by the presence of cytoplasmic membranes with sufficiently low conductivity. Since the conductivity of the cell wall was found to be of nearly the same value as that of the suspending medium, composed of Kcl solutions in a range from 10 to 80 mM, the cell wall may be ignored in establishing an electrical model of the cells suspended in such media. An analysis of the dielectric data was carried out by use of Pauly and Schwan's theory. The membrane capacitance was estimated to be 1.1+/-0.1 muF/cm2, which is compared with values reported so far for most biological membranes. The conductivity of the cell interior was almost unchanged with varying KCl concentrations and showed low values owing to the presence of less conducting particles, presumably intracellular organelles. The relatively low dielectric constant of about 50 obtained for the cell interior, in comparison with values of aqueous solutions, may be attributed also to the presence of intracellular organelles and proteins.

Cell Membrane↗

Current source design for electrical impedance tomography.

Questions regarding the feasibility of using electrical impedance tomography (EIT) to detect breast cancer may be answered by building a sufficiently precise multiple frequency EIT instrument. Current sources are desirable for this application, yet no current source designs have been reported that have the required precision at the multiple frequencies needed. We have designed an EIT current source using an enhanced Howland topology in parallel with a generalized impedance converter (GIC). This combination allows for nearly independent adjustment of output resistance and output capacitance, resulting in simulated output impedances in excess of 2 Gohms between 100 Hz and 1 MHz. In this paper, the theoretical operation of this current source is explained, and experimental results demonstrate the feasibility of creating a high precision, multiple frequency, capacitance compensated current source for EIT applications.

Breast Neoplasms↗

Forces on biological cells due to applied alternating (AC) electric fields. II. Electro-rotation.

Measurements are presented of angular velocities of rotation of mammalian cells of K562 (human) and SP2 (mouse) in external alternating electric fields over a frequency range of 0.5 kHz to 12 MHz. Electro-rotation of the cells was observed for the case of "two cells in contact' using two parallel, cylindrical electrodes; only one cell was located on the electrode. A theoretical analysis is also presented which shows that the cell rotation arises from a torque produced by the interaction between the primary electric dipole moment induced in the spinning cell and the secondary electric fields, generated by the primary dipole induced in the adjacent cell. These secondary fields are out of phase with the applied electric field. The results show that (a) only the cell not located on the electrode rotates, (b) maximal electro-rotation occurs at two different excitation field frequency domains for the frequency range employed here, (c) the spin speed of the rotating cell at each frequency domain is much less than the excitation frequency, (d) the rotation direction of the cell depends on the angle (theta) between the external electric field and the line joining the centres of the two cells and (e) for a given angle theta, the rotation direction is the same for both excitation frequency domains. The experimental measurements allowed us to estimate the conductivities of the cytoplasms and membrane capacitances of the cells of K562 and SP2. The conductivities of the cytoplasms of the cells of K562 and SP2 were estimated to be 0.2 and 0.3 Sm-(1), respectively, whereas the membrane capacitances of these cells were found to be 2.7 +/- 0.8 and 9.8 +/- 0.6 mFm-(2), respectively.

Animals↗

AAEM minimonograph #36: basic concepts of electricity and electronics in clinical electromyography.

Fundamental principles of electricity provide a basis for understanding the design and operation of electromyography equipment. An intuitive and quantitative explanation of charge, voltage, current, and impedance provides an introduction to the concepts of resistance, capacitance, and input impedance. These concepts form the basis for discussion of filters, amplifiers, electrodes, digital electronics, stimulators, and patient safety. The monograph assumes no specialized training in engineering or mathematics. The topics are discussed at an introductory level to provide understanding for readers with no electronics background and intuitive insight for more experienced readers.

Electric Conductivity↗

RF performance of a 418-MHz radio telemeter packaged for human vaginal placement.

The electrical and communication performance of a 0.8-microW UHF temperature telemeter designed for human vaginal placement is discussed; a solenoidal loop antenna was used, occupying a volume of 0.1 cm3. In situ, measured power absorption was between 19-25 dB, resulting in an effective operating range of 10 m. Capacitive loading lowered the antenna's resonant frequency by 1.4% and there was a significant polarization change in the radiated output.

Electric Conductivity↗

Electrical interfacing of nerve cells and semiconductor chips.

The electrical interfacing of individual nerve cells and silicon microstructures is considered, as well as the assembly of elementary hybrid systems made of neuronal networks and semiconductor microelectronics. Without electrochemical processes, coupling of the electron-conducting semiconductor and the ion-conducting neurons relies on a close contact of cell membrane and oxidised silicon with a high resistance of the junction and a high conductance of the attached membrane. Neuronal excitation can be elicited and recorded from the chip by capacitive contacts and by field-effect transistors with an open gate. Integrated iono-electronic system are obtained by the outgrowth of neuronal networks on the surface of the silicon chip, by implementing electrical circuits in the chip and by two-way interfacing of the neuronal and the electronic components.

Animals↗

Capacitive-coupling-enhanced switching gain in an electron y-branch switch.

We have fabricated electron Y-branch switches (YBS) on modulation doped GaAs/AlGaAs heterostructures. The Y branch consists of a one-dimensional source, which is split along the branching section into two one-dimensional drains. In addition to source drain voltages, external electric fields can be applied via gates along the branches. In the nonlinear transport regime sweeps of the side-gate voltages lead to a voltage difference between the drain reservoirs with gain. This switching gain increases superlinearly with the bias voltage applied between the source and the drains of the YBS. We explain the bias voltage enhanced switching by a capacitive coupling of the branches.

Journal Article↗

Effects of protein phosphorylation on the regulation of capacitative calcium influx in Xenopus oocytes.

The regulation of capacitative Ca2+ influx in Xenopus oocytes was investigated using both the two electrode voltage-clamp (where Ca2+ is monitored through the Ca2+-dependent Cl- current) and patch-clamp techniques. Following stimulation of expressed 5-hydroxytryptamine (5-HT) receptors, capacitative Ca2+ influx deactivated in around 15 min. Following injection of [adenosine 5'-O-(3-Thiotriphosphate)] (ATP [gamma-S]), an ATP analogue that is readily used by protein kinases, capacitative Ca2+ influx activated by 5-HT application either did not deactivate or was prolonged around twofold. However, injection of adenylyl 5'-(beta,gamma-methylene)-diphosphonate (AMP-PCP), another ATP analogue that is not utilised by kinases, did not affect the time-course of Ca2+ influx. When capacitative Ca2+ influx was activated by readmission of Ca2+ to oocytes incubated in thapsigargin/0 Ca2+ solution for several hours, Ca2+ influx occurred and a weakly saturating relationship between external Ca2+ and Ca2+ influx was found. Ca2+ influx in thapsigargin-treated cells was unaffected by ATP [gamma-S]. ATP [gamma-s] and several kinases had no effect on the Ca2+-dependent Cl- current when the latter was activated by elevation of Ca2+ independent of capacitative Ca2+ influx. Protein kinase C slowly and partially inhibited the Cl- current. Outside-out patches taken from thapsigargin-treated cells failed to demonstrated any Ca2+ current or Ca2+-dependent Cl- current on reapplying high Ca2+ to the patch, despite the oocyte showing a large capacitative Ca2+ influx. The results suggest that a kinase, activated on receptor stimulation, prolongs the activation time-course of capacitative Ca2+ influx.

Adenosine Triphosphate↗

An optimized approach to membrane capacitance estimation using dual-frequency excitation.

We present an optimized solution to the problem of membrane impedance estimation when a patch-clamped cell is stimulated by a dual-frequency, sinusoidal excitation. The complete data set of raw whole-cell current samples is typically reduced, via digital lock-in detection, to measurements of the complex cell model admittance at the two stimulus frequencies. We describe a statistical model of both data sets and demonstrate that the admittance data adequately represent the essential features obtained from the raw data. The parameter estimates obtained by a nonlinear weighted least-squares solution (NWLS), which under normal recording conditions is equivalent to the maximum likelihood solution, essentially obtain the theoretical lower bound on variance established by the Cramér-Rao bound. Our software implementation of the NWLS solution produces estimates of the cell model parameters that are less noisy than other dual-frequency systems. Our system can be used 1) to measure slow changes in membrane capacitance-in the face of large, slow changes in membrane resistance, 2) to detect with confidence capacitance changes expected from the exocytosis of moderate-sized dense core granules, and 3) to reduce the cross-talk between transient changes in membrane conductance and membrane capacitance.

Adrenal Medulla↗

Membrane capacitance changes associated with particle uptake during phagocytosis in macrophages.

We report the use of capacitance measurements to monitor particle uptake after cellular exposure to phagocytic stimuli. In these studies, human monocyte-derived macrophages (HMDMs) and cells from the murine macrophage-like cell line J774.1 were exposed to immune complexes or sized latex particles (0.8 or 3.2 micron in diameter). An average decrease in cell capacitance of 8 pF was seen after exposure of the cells to immune complexes. Cells in which particle uptake was inhibited by cytochalasin B treatment before exposure to immune complexes showed an average increase of 0.5 pF. The decrease in membrane capacitance after exposure of cells to particulate stimuli was absent with the soluble stimulus, platelet-activating factor, further confirming that decreases in membrane capacitance were due to particle uptake. Exposure of cells to sized latex particles resulted in a graded, stepwise decrease in membrane capacitance. The average step size for 0.8-micron particles was 250 fF, and the average step change for the larger 3.2-micron particles was 480 fF, as calculated from Gaussian fits to the step size amplitude histograms. The predicted step size for the individual particles based upon the minimum amount of membrane required to enclose a particle and a specific capacitance of 10 fF/micron2 was 20 and 320 fF, respectively. The step size for the smaller particles deviates significantly from the predicted size distribution, indicating either a possible lower limit to the size of the phagocytic vacuole or multiple particles taken up within a single phagosome. Dynamic interaction between phagocytosis and exocytosis was observed in a number of cells as a biphasic response consisting of an initial rapid increase in capacitance, consistent with cellular exocytosis, followed by stepwise decreases in capacitance.

Animals↗

Myocyte electrophysiological properties following the development of supraventricular tachycardia-induced cardiomyopathy.

Chronic supraventricular tachycardia (SVT) causes left ventricular (LV) dilatation and dysfunction, diminished myocyte contractile function, and abnormalities in sarcolemmal receptor systems. We hypothesized that changes in myocyte action potential characteristics and L-type Ca2+ channel (Ca2+ channel) function, which are major determinants of myocyte contractile processes, would occur with SVT cardiomyopathy. LV function and isolated myocyte contractile function were examined in 11 pigs with SVT cardiomyopathy (pace 240 bpm; 3 weeks) and 11 control pigs. With chronic SVT, LV fractional shortening fell and myocyte shortening velocity was reduced compared to controls (11 +/- 2 v 37 +/- 2%, P < 0.0001; and 32.5 +/- 1.2 v 55.7 +/- 1.6 microns/s, P < 0.0001, respectively). Isolated myocyte action potential upstroke velocity and amplitude were reduced with SVT cardiomyopathy compared to controls (92.8 +/- 4.8 v 129.5 +/- 3.1 V/s, P < 0.0001; and 98.2 +/- 2.2 v 110.3 +/- 1.3 mV, P < 0.0001, respectively). the duration of the myocyte action potential, defined as the time to 90% repolarization, was prolonged with SVT cardiomyopathy compared to controls (201.7 +/- 5.9 v 169.1 +/- 6.8 ms, P = 0.002). These specific abnormalities in the indices of myocyte contractile function and action potential characteristics which occurred with SVT cardiomyopathy were not normalized following beta-adrenergic receptor stimulation. In order to determine a potential mechanism for the changes in myocyte contractile function and action potential characteristics with SVT cardiomyopathy, Ca2+ channel function was examined in control and SVT myocytes. In SVT myocytes, peak L-type Ca2+ current (ICa) normalized to membrane capacitance and the Ca2+ channel inactivation time constant were reduced compared to controls (-2.30 +/- 0.24 v -3.79 +/- 0.28 pA/pF, P = 0.0001; and 104.0 +/- 10.8 v 199.9 +/- 27.4 ms, P = 0.005, respectively). The abnormalities in Ca2+ channel function with SVT cardiomyopathy persisted in myocytes with equivalent membrane capacitances and were not normalized with beta-adrenergic receptor stimulation. In conclusion, findings from the present study suggest that fundamental abnormalities in myocyte electrical events (action potential) and ionic flux (Ca2+ channel function) are contributory mechanisms for the depressed myocyte contractile function with SVT cardiomyopathy.

Action Potentials↗

Electrode-skin impedance from a dielectric viewpoint.

It is well established that the frequency variation of the complex electrode-skin impedance (ESI) Z is given by Z = K(i omega)-n, where n has a typical value of about 0.8. It is not easy to find a physical reason for this behaviour. However, many materials exhibit Cole-Davidson (CD) behaviour, so that in the high-frequency (hf) region their impedance has a characteristic which is almost indistinguishable from that of the ESI. It is therefore possible that the ESI exhibits a behaviour given by the CD equation or one of its variants, but that this has not been detected because experimental measurements have not extended to sufficiently low frequencies. Whatever the ESI behaviour may be at low frequencies, there are theoretical reasons which show that Z cannot obey the above equation indefinitely as the frequency is reduced. If the deviation from it at low frequencies were found to be consistent with CD behaviour, then a physical explanation in terms of protonic fluctuations, which has previously been put forward to explain CD behaviour, would be available as a possible explanation of ESI behaviour over the whole frequency range. It is shown that any such deviation would be more likely to be detected if the variation of complex capacitance with frequency, rather than of impedance with frequency, were to be plotted.

Electric Impedance↗

Plasma endothelin-1 during central hypovolaemia in man.

Endothelin-1 (ET-1) is a potent vasoconstricting peptide with effect on resistance as well as capacitance vessels. We followed ET-1 in arterial plasma together with heart rate (HR), central venous pressure (CVP), mean arterial pressure (MAP), and thoracic electrical impedance (TI) in seven men during central hypovolaemia induced by 50 degrees head-up tilt. During tilting plasma ET-1 increased from 1.1 +/- 0.2 to 1.4 +/- 0.3 pmol l-1 (mean +/- SE) concomitant with an increase in total peripheral resistance (TPR) (from 15 +/- 2 to 25 +/- 3 mmHg min l-1) (P < 0.01), and HR (from 67 +/- 2 to 94 +/- 5 beats min-1) (P < 0.01) while MAP remained unchanged. CVP decreased (from 1.8 +/- 0.9 to -1.6 +/- 1.0 mmHg) (P < 0.01) during tilting and remained unchanged during sustained tilt despite further reduction of central blood volume as recorded by TI. Presyncopal symptoms occurred after 28 +/- 6 min associated with decreases in HR (to 70 +/- 6 beats min-1), MAP (from 90 +/- 3 to 52 +/- 4 mmHg) and TPR (to 11 +/- 2 mmHg min l-1) (P < 0.01). At this time plasma ET-1 reached its highest level of 1.6 +/- 0.3 pmol l-1 (P < 0.01). Data show that head-up tilt is associated with increased plasma concentrations of ET-1 which may play a role in maintaining vascular tone in situations with a reduced central blood volume.

Adult↗