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Development of nitrogen-removal bioreactor using poly(lactic acid) as an energy source.

To control nitrogen such as ammonia in a rearing water of aquatic animals, we developed new bioreactor capable of both nitrification and denitrification. It was consisted of gel-plate immobilized microorganisms and a biodegradable plastic plate composed of three kind of poly(lactic acid) as an energy source of denitrification. When batch treatment experiment by the bioreactor was continuously carried out with an artificial rearing water containing ammonia, nitrogen-removal rate of the bioreactor was approximately 3 g-N/d/m2-gel surface and the activity was maintained for over 3 month without additional energy source. Therefore, the bioreactor would be effective to control nitrogen concentration in rearing water of a closed water circulating system for aquatic animals.

Ammonia↗

Review article: structural and functional properties of cytochrome aa3 from bacteria.

The aa3 oxidases from bacteria form a group of related enzymes that resemble the far more complex mitochondrial cytochrome c oxidase, both functionally and structurally. These enzymes catalyze electron transfer from ferrocytochrome c to oxygen to produce water. This transfer is coupled to proton translocation. Several oxidases of this type have been purified from cytoplasmic membranes of bacteria. This review summarizes the present knowledge on purified bacterial aa3 oxidases and correlates these findings with data available for the eukaryotic cytochrome c-oxidases.

Bacteria↗

[Bacterial lipids].

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Bacillus subtilis↗

[Relationship of hydrogen bacteria to carbon monoxide].

The purpose of this work was to study the ability of various cultures of hydrogen bacteria to grow in a gaseous mixture containing, apart from H2, O2 and CO2, carbon monoxide at a concentration of 10% (v/v) and higher. In contrast to CO-oxidizing bacteria, these organisms could not use CO as a sole source of energy. Among 17 studied strains of hydrogen bacteria, only those belonging to the group Alcaligenes eutrophus could withstand 10% of CO (v/v) in a gaseous mixture.

Alcaligenes↗

Phase separation of integral membrane proteins in Triton X-114 solution.

A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic proteins are found exclusively in the aqueous phase, and integral membrane proteins with an amphiphilic nature are recovered in the detergent phase. Triton X-114 is used to solubilize membranes and whole cells, and the soluble material is submitted to phase separation. Integral membrane proteins can thus be separated from hydrophilic proteins and identified as such in crude membrane or cellular detergent extracts.

Acetylcholinesterase↗

[No stimulation of the growth of microorganisms by repair-substances (author's transl)].

By means of manometric technique the influence of X-ray-repairsubstances on the growth and respiration of Micrococcus denitrificans, Micrococcus radiodurans and Saccharomyces cerevisiae was investigated. Though these substances are stimulating the development of higher plants, there is no effect on growth and respiration of these microorganisms.

Micrococcus↗