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Oral electrochemical action after soft drink rinsing and consumption of sweets.

The aim of this study was to investigate the influence of pH changes in dental plaque and saliva on the magnitude of the galvanic current created when amalgam restorations make contact in the oral cavity. Ten persons with 46 contacts between amalgam fillings in all participated in the experiments. Potential, polarization, and pH measurements were performed before and after Coca-Cola and orange juice rinsing and intake of sweets, which were used as test products. Distilled water was used as a control. The measurements were performed 2, 5, 10, 20, and 30 min after the rinsing or intake. There was no statistically significant difference in the current magnitude after any test product or between the time intervals after the different products. The results indicated that changes of the plaque and saliva pH for a short time after food and soft drink intake do not influence the magnitude of the galvanic current flowing between amalgam restorations in contact.

Adult↗

Histopathology of melanocytic lesions in goats and establishment of a melanoma cell line: a potential model for human melanoma.

Melanocytic cells from white Angora goats were studied in vivo and in vitro. The histopathology of pigmented areas of skin from the most common sites of melanoma (solar-exposed areas of the ear, face, and perineum) resembled that of the epidermal melanocytes in Hutchinson's melanotic freckle in humans. Seven melanoma biopsies from 6 Angora goats showed histopathological features in common with human melanoma. A melanoma cell line, GM-1, was established in culture from a lymph node metastasis obtained from an animal that had a primary tumor excised and later developed extensive metastatic disease. GM-1 cells were mainly diploid, amelanotic, proliferated rapidly, spontaneously formed vacuolated cells, and were tumorigenic in nude mice. The species of origin of the GM-1 line was confirmed by isozyme profiles. GM-1 cultured cells and the original biopsy both expressed S-100 protein and tyrosinase antigen. Using GM-1 cells as the immunogen, a monoclonal antibody (MoAb 1F1) was derived that reacted strongly with a 116 kDa antigen in 50% of the GM-1 cells, but had little activity with goat fibroblasts (GM-F) or with human melanoma cells. GM-F, on the other hand, yielded more intense staining than GM-1 with an intermediate filament antibody (IFA), reacting with a 58 kDa antigen in both cell lines. The sensitivity of GM-1 to anticancer agents was similar to that of human melanoma cells. The pathology of caprine melanoma and its association with sun-exposed sites in relatively young animals suggest that it may be a suitable model for studying induction of melanoma by natural sunlight.

Animals↗

Stimulation of inositol phospholipid breakdown in rat cortical and hippocampal miniprisms by noradrenaline, 5-hydroxytryptamine and carbachol: some methodological aspects.

After incubation of miniprisms from rat cerebral cortex or hippocampus with 3H-myo-inositol, labelling of the phospholipid ("Lipid") and inositol phosphate ("InsP") fractions was found. Inositol phospholipid hydrolysis ("PI breakdown") was stimulated by noradrenaline, 5-hydroxytryptamine and carbachol. Expressing data as InsP/(Lipid + InsP) was found to be a superior measure of the rate of PI breakdown compared with the more commonly used InsP d.p.m. unit, since the former was found to be independent of the volume of the miniprism aliquot used and the degree of labelling of inositol phospholipids. The PI breakdown responses to noradrenaline, 5-hydroxytryptamine and particularly carbachol were found to be enhanced by increasing the assay [K+] from 5.88 mM to 18.2 mM. Storage of hippocampal samples at -70 degrees by the "slow freeze-fast thaw" method of Hardy et al. (1983) resulted in a decreased degree of labelling of the Lipid and InsP fractions and a loss of the PI response to noradrenaline when assayed at a [K+] of 5.88 mM, but a reasonable response was seen in these samples at an assay [K+] of 18.2 mM. The temperature of the Krebs-Henseleit buffer used in the preparation of the miniprisms was found to be important for the PI breakdown response.

Animals↗

Laboratory studies of sweets re-formulated to improve their dental properties.

OBJECTIVES: To evaluate the potential dental effects of ten new types of sugar-free sweets formulated with Lycasin or isomalt as bulk sweeteners instead of sugars. METHODS: Examination of the sweets for their acidity, fermentability by oral microorganisms, influence on the demineralisation of dental enamel, and their influence on human interdental plaque pH, compared with conventional sugar-containing sweets. FINDINGS: The importance of reducing the levels of flavouring acids in the sweets was demonstrated. It was not straightforward to evaluate chocolate products in this system, but the potential benefits of re-formulating fruit gums, lollipops, chew-bars, toffee and fudge with Lycasin or isomalt in place of sugars were shown by determining their reduced acidogenicity and fermentability compared with conventional confectionery. CONCLUSIONS: The extent of demineralisation of dental enamel was related to both the acidity and the fermentability of the sweets. Re-formulating sweets with reduced acidity levels and bulk sweeteners not fermentable by dental plaque microorganisms can provide a basis for improving their potential dental effects.

Analysis of Variance↗

Said another way. My definition of nursing.

The definition of nursing has evolved since the introduction of the definition by the American Nurses Association in 1980. The society we nurses serve has changed significantly and members of the society demand quality health care and respect for their individual decisions regarding their health care. As a profession, we must keep our fingers on the pulse of society and be ready to respond to the changes of society's needs. Being diligent in defining our position in society is imperative for the preservation of the nursing profession. This paper supports the current definition of the ANA (2003) within the domains of the art, science, and practice of nursing.

American Nurses' Association↗

Dental caries risk in relation to dietary habits and dental services in two industrial populations.

OBJECTIVES: Environmental hazards contribute to the poor oral health in many occupations. Among the earliest occupations mentioned are those of the baker and confectionery worker. To determine appropriate ways of promoting oral health in places of work, factors affecting caries susceptibility were studied. METHODS: Dental caries status, dietary and dental health behaviors, and salivary microbiologic findings in 338 confectionery and 101 shipyard workers were compared. A caries risk index based on salivary findings was used in log-linear models where the effects of sex, diet, use of dental services, and work environment were studied. RESULTS: Caries experience was found to be high in both populations. Dietary habits were the most important factor affecting caries occurrence and susceptibility. Similar dietary behaviors were found in both groups. CONCLUSIONS: The confectionery industry did not seem to be an exceptionally hazardous environment for dental health in general. However, the screening of high-risk workers should be organized. Reimbursement of costs had no major effect on use of dental services or caries risk. Oral health promotion should be integrated with existing occupational health services to improve oral health in industrial populations.

Adult↗

Oral health care knowledge and practices of a group of deaf adolescents in Lagos, Nigeria.

OBJECTIVE: This study sought to determine the oral health care knowledge and practices of a group of deaf adolescents in Lagos. METHODS: The study involved 50 students of Wesley School 1 for the Deaf, Lagos (26 males and 24 females, aged 10-19 years, mean 13.3 +/- 2.8). Information about previous dental care, oral hygiene, and snacking habits were obtained through a questionnaire and sign language by the teachers. RESULTS: Only 12 percent of pupils had received dental care. Eight percent and 72 percent, respectively, gave correct answers to causes of tooth decay and bleeding gums. Ninety-four percent brushed their teeth once daily, with no significant sex difference (P > .05). Reported dental problems include bleeding gums (36%), tooth discoloration, and tooth decay. The majority of pupils (60%) preferred biscuits and soft drinks as snacks. More than 90 percent were willing to have a dental check-up. CONCLUSIONS: The oral health knowledge and practices of this group of children will improve through a controlled school-based oral health education program.

Adolescent↗

Comparison between saliva stimulants and a saliva substitute in patients with xerostomia and hyposalivation.

The purpose of this study was to assess patient preference and product efficacy of three non-prescription products for the symptomatic relief of xerostomia. The study group consisted of 80 individuals with a complaint of chronic (> six months) xerostomia and an unstimulated salivary flow rate of < 0.1 mL/min. The three products--a sorbitol/xylitol-sweetened chewing gum, a sorbitol-sweetened sour lemon lozenge, and a sorbitol/xylitol-sweetened artificial saliva substitute spray--were assigned in a permuted block randomization scheme. Each product was used for two weeks with an interval of one week between trials. The study did not identify any product to be statistically significant in terms of patient preference. Kruskal-Wallis testing revealed no statistical significance (P > 0.589) among the products. No product demonstrated marked efficacy in stimulating salivary output. ANOVA analysis followed by Tukey HSD testing revealed no significant difference between the baseline paraffin-stimulated mean flow rate and the gum- and lozenge-stimulated flow rates.

Adult↗