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PubMed2006

Correction of presbyopia by technovision central multifocal LASIK (presbyLASIK).

PURPOSE: To investigate central multifocal presbyLASIK based on the creation of a central hyperpositive area. METHODS: Twenty-five patients (50 eyes) underwent presbyLASlK in an open-label, prospective, non-comparative pilot study. Mean patient age was 58 years (range: 51 to 68 years), mean preoperative spherical equivalent refraction was +1.6 +/- 0.63 diopters (D) (range: +0.50 to +3.00 D), and mean spectacle near addition was +2.27 +/- 0.37 D (range: +1.75 to +3.00 D). The ablation pattern was performed with proprietary software from Technovision using an H. Eye Tech. excimer laser platform. RESULTS: Mean postoperative spherical equivalent refraction was -0.37 +/- 0.55 D (range: -1.50 to + 1.00 D) and mean spectacle near addition was +1.72 +/- 0.34 D (range: +1.25 to +2.25 D). After 6 months, 16 (64%) patients achieved a distance uncorrected visual acuity (UCVA) of > or = 20/20 and 18 (72%) patients achieved a near UCVA of > or = 20/40. Seven (28%) patients lost a maximum of 2 lines of best spectacle-corrected visual acuity (BSCVA). The safety index for distance was 0.98 binocular and for near was 0.99 binocular. After 6 months, no significant change was noted in contrast sensitivity at 1.5 cycles/degree. A significant mean reduction was found at spatial frequencies of 3, 6, 12, and 18 cycles/degree (P<.001). There was a significant change in corneal aberrations after surgery. The coefficients for coma increased and the coefficients for spherical aberrations decreased. A significant decrease was noted in point spread function values (P=.0018). CONCLUSIONS: Central presbyLASIK may be used to provide improvement in functional near vision in patients with presbyopia associated with low and moderate hyperopia. However, factors involved in the loss of BSCVA in some cases and loss in vision quality should be further clarified prior to its general use.

Adult↗
PubMed2005

Phosphorylated l-caldesmon is involved in disassembly of actin stress fibers and postmitotic spreading.

The function of the ubiquitous actin-binding protein, caldesmon (l-CaD) in mammalian non-muscle cells remains elusive. During mitosis, l-CaD becomes markedly phosphorylated at Ser497 and Ser527 (in the rat sequence), therefore, it has been suggested that l-CaD is involved in cytokinesis by inhibiting the actomyosin interaction until it is phosphorylated, although direct in vivo evidence is still missing. In the present study, we used F-actin staining and specific antibodies against these two phosphorylation sites of l-CaD to simultaneously monitor actin assembly and l-CaD phosphorylation. Our observations demonstrated that the level of l-CaD phosphorylation undergoes dynamic changes during the cell cycle. The spatial and temporal distributions of phospho-CaD do not correlate with cytokinesis per se, but rather, with the level of actin bundles in a reciprocal manner. The highest l-CaD phosphorylation level coincides with the disassembly of actin cytoskeleton during mitotic cell rounding. Ser-to-Ala mutations at these two positions prevent stress fibers from disassembly upon migratory stimulation. In addition, phospho-CaD appears to colocalize with nascent focal adhesion complexes during postmitotic spreading. These findings suggest that l-CaD phosphorylation plays an important role not only in cytoskeleton remodeling during cell shape changes, but also in cell spreading and migration.

Actins↗
PubMed2006

Origin sites of spontaneous cortical spreading depression migrated during focal cerebral ischemia in rats.

Spontaneous cortical spreading depression (CSD) has been found to occur in the penumbral zone of the brain in rats with focal cerebral ischemia, and has been shown to promote expansion of infarction. Electrophysiological recording of CSD has been used for monitoring the penumbral zone, but with an inherently low spatial resolution; consequently, optical intrinsic signal imaging (OISI) was applied to characterize the spontaneous CSD waves following permanent left middle cerebral artery occlusion (MCAO) in rats under alpha-chloralose/urethane anesthesia. Besides the previous report about the regional variation of optical reflectance during spontaneous CSD following MCAO, the origin site of CSD was easily determined using OISI with the benefit of high resolution in the present study. Those origin points (n = 82) were dynamically located in the ipsilateral hemisphere cortex: sometimes outside of the 6 mm x 8 mm observation area in the parietal cortex (n = 19, 23%), and sometimes inside (n = 63, 77%). The data showed a general trend towards the medial cortex (0.40 +/- 0.15 mm per CSD). Because the lateral cortex of the rat brain proved to be infarcted with 2% 2,3,5-triphenyltetrazolium chloride (TTC) staining after 4 h occlusion, the migration of the origin sites implied a growth of the infarcted area. Hence, the determination of origins of spontaneous CSD using OISI would contribute to the continued study of stroke.

Animals↗
PubMed2001

Optical recording of spreading depression in rat neocortical slices.

A spreading depression (SD) was elicited in adult rat neocortical slices by microdrop application of high potassium and the SD propagation pattern was analyzed by recording simultaneously the extracellular DC potential and the changes in the intrinsic optical signal. The electrical SD with an average peak amplitude of 13.2+/-3.4 mV showed a good spatial and temporal correlation with the optical signal. In 79% of the slices, the SD was characterized by an initial increase of light reflectance by 2.3+/-1.6%, followed by a reflectance decrease of 0.5+/-2.4% and finally a larger and long-lasting increase by 5+/-2.4%. In the remaining slices, the SD revealed an initial decrease in light reflectance by 5.8+/-1.8% followed by an increase of 1.4+/-1.2%. In all slices, the recovery in the DC recording was faster as in the optical signal. The SD preferentially propagated within layers I-IV and could be blocked in most experiments by a vertical incision through upper layers or by local glutamate receptor blockade following microdrop application of kynurenic acid in layers II-III. The SD could be also blocked by bath application of kynurenic acid, MK-801 and octanol, but not by the more specific gap junction blocker carbenoxolone. Our results indicate that the high density of dendritic processes and glutamate receptors in layers II-IV promote the horizontal spread of the SD in these cortical layers and that gap junctions are not required for the propagation of SD in neocortical slices.

Animals↗
PubMed1996

Spatial and temporal variations in pacemaking and conduction in the isolated renal pelvis.

In renal pelvis preparations isolated from the sheep, the location of the pacemaker and the pathway of conduction of the electrical impulse in the pelvis were analyzed in detail. An electrophysiological acquisition system was used allowing simultaneous recordings from 240 extracellular electrodes. Reconstruction of the spread of activity showed that the site of the pelvis pacemaker was, in virtually all cases, located at the pelvicalyceal border and never in the body of the pelvis or in the area of the pelviureteric junction. One single pacemaker was responsible for a particular spread of activation, and fusion of activity originating from two or more pacemakers did not place. Furthermore, spontaneous shifts of the pacemaker could occur from one site to another along the pelvicalyceal border. Conduction from the site of the current pacemaker to the pelviureteric junction and the ureter was slow, inhomogeneous, and contorted. Multiple instances of partial or total conduction block were seen at all levels in the pelvis and were not restricted to the pelviureteric junction. The occurrence of the conduction block did not seem to be related to the length of the preceding interval, implying that the refractory period did not play a major role in the genesis of intrapelvic conduction block. In conclusion, high-resolution mapping of the renal pelvis is possible and reveals location and behavior of the pacemaker and documents inhomogeneities in conduction and conduction block.

Animals↗
PubMed1977

Visualization of centriole-bodies using silver stain.

Duplicate staining of human metaphase chromosomes, first with Giemsa followed by silver, revealed the presence of two small silver-stained bodies not seen in the Giemsa stained metaphases. Similar bodies were subsequently found in the metaphases of several animal groups. The size, structure, spatial relationships to the nucleus, behavior throughout the cell cycle, and apparent universal presence of these bodies suggest that they are either centrioles or associated centriolar structures. These centriole-bodies can be seen throughout the cell cycle, even in hypotonically spread C-metaphase chromosome plates. The silver stain procedure allows enough resolution to distinguish parent and daughter centriole-bodies at interphase, thus permitting visualization of the replication, maturation and separation stages of these bodies with the light microscope.

Animals↗
PubMed1995

Dispersal and mobility of heavy metals in relation to tree survival in an aerially contaminated woodland soil.

Nearly a century of metal deposition adjacent to a metal refinery in Prescot, north-west England has led to highly elevated metal levels in soils at a dominantly Acer pseudoplatanus woodland, but with incongruously and perplexingly few detrimental effects on trees. Dispersal and speciation of Cu, Cd, Zn, Pb and Ni in soil was found to be extremely variable, but spatial patterns of metals were inter-related and also related to soil pH and soil organic matter. These soil variables were all generally higher in soil directly beneath trees than in soil between trees, and were particularly high beneath the spreading canopy of Aesculus hippocastanum. It is argued that this heterogeneous dispersal and availability of metals in soils may explain the survival of mature trees and the successful establishment of seedlings within the woodland. Differing speciation and mobility has allowed high disappearance rates of metals since recent closure of the refinery, which may result in soil recovery at a faster rate than previously thought.

Journal Article↗
PubMed2006

Achieving centimetre-scale supercollimation in a large-area two-dimensional photonic crystal.

Diffraction, a fundamental process in wave physics, leads to spreading of the optical beams as they propagate. However, new photonic crystal (PhC) meta-materials can be nano-engineered to generate extreme anisotropy, resulting in apparent propagation of light without diffraction. This surprising phenomenon, called supercollimation, effectively freezes the spatial width of a light beam inside a PhC, observed over a few isotropic diffraction-lengths. However, using such experiments to predict the behaviour for longer propagation lengths is difficult, as a tiny error in a measured width can extrapolate to order unity uncertainty in the width at distances over hundreds of diffraction-lengths. Here, supercollimation is demonstrated in a macroscopic PhC system over centimetre-scale distances, retaining spatial width confinement without the need for waveguides or nonlinearities. Through quantitative studies of the beam evolution in a two-dimensional PhC, we find that supercollimation possesses unexpected but inherent robustness with respect to short-scale disorder such as fabrication roughness, enabling supercollimation over 600 isotropic diffraction-lengths. The effects of disorder are identified through experiments and understood through rigorous simulations. In addition, a supercollimation steering capability is proposed.

Journal Article↗
PubMed1992

Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos.

The Drosophila gene pumilio is expressed maternally, and its function is essential during early embryogenesis for the formation of abdominal segments. Our molecular analysis reveals that pumilio is a large gene that encodes a protein of 160 kD whose RNA is enriched at the posterior pole of the egg. As with pumilio, the maternal effect gene nanos is specifically required for abdomen formation. The Nanos protein is expressed in a posterior-to-anterior concentration gradient in the developing embryo. Previous experiments demonstrated a genetic interaction between pumilio and nanos, and led to the suggestion that pumilio is required for the proper spatial distribution of the Nanos protein. Here, we show that the expression and distribution of nanos RNA and protein in embryos derived from pumilio mutant females are indistinguishable from wild type. We conclude that abdomen formation depends both on Nanos activity, spreading from the localized posterior source, and on Pumilio activity, present throughout the embryo.

Abdomen↗
PubMed2005

The use of film dosimetry of the penumbra region to improve the accuracy of intensity modulated radiotherapy.

Accurate measurements of the penumbra region are important for the proper modeling of the radiation beam for linear accelerator-based intensity modulated radiation therapy. The usual data collection technique with a standard ionization chamber artificially broadens the measured beam penumbrae due to volume effects. The larger the chamber, the greater is the spurious increase in penumbra width. This leads to inaccuracies in dose calculations of small fields, including small fields or beam segments used in IMRT. This source of error can be rectified by the use of film dosimetry for penumbra measurements because of its high spatial resolution. The accuracy of IMRT calculations with a pencil beam convolution model in a commercial treatment planning system was examined using commissioning data with and without the benefit of film dosimetry of the beam penumbrae. A set of dose-spread kernels of the pencil beam model was calculated based on commissioning data that included beam profiles gathered with a 0.6-cm-i.d. ionization chamber. A second set of dose-spread kernels was calculated using the same commissioning data with the exception of the penumbrae, which were measured with radiographic film. The average decrease in the measured width of the 80%-20% penumbrae of various square fields of size 3-40 cm, at 5 cm depth in water-equivalent plastic was 0.27 cm. Calculations using the pencil beam model after it was re-commissioned using film dosimetry of the penumbrae gave better agreement with measurements of IMRT fields, including superior reproduction of high dose gradient regions and dose extrema. These results show that accurately measuring the beam penumbrae improves the accuracy of the dose distributions predicted by the treatment planning system and thus is important when commissioning beam models used for IMRT.

Film Dosimetry↗
PubMed1990

Subcellular calcium transients visualized by confocal microscopy in a voltage-clamped vertebrate neuron.

Confocal laser-scanned microscopy and long-wavelength calcium (Ca2+) indicators were combined to monitor both sustained and rapidly dissipating Ca2+ gradients in voltage-clamped sympathetic neurons isolated from the bullfrog. After a brief activation of voltage-dependent Ca2+ channels, Ca2+ spreads inwardly, and reaches the center of these spherical cells in about 300 milliseconds. Although the Ca2+ redistribution in the bulk of the cytosol could be accounted for with a radial diffusion model, local nonlinearities, suggesting either nonuniform Ca2+ entry or spatial buffering, could be seen. After electrical stimulation, Ca2+ signals in the nucleus were consistently larger and decayed more slowly than those in the cytosol. A similar behavior was observed when release of intracellular Ca2+ was induced by caffeine, suggesting that in both cases large responses originate from Ca2+ release sites near or within the nucleus. These results are consistent with an amplification mechanism involving Ca2(+)-induced Ca2+ release, which could be relevant to activity-dependent, Ca2(+)-regulated nuclear events.

Animals↗
PubMed2001

Gene expression profiling of acute spinal cord injury reveals spreading inflammatory signals and neuron loss.

We have completed the first large-scale gene expression study of acute spinal cord injury (SCI) in rat. Oligonucleotide microarrays containing 1,200 gene-specific probes were used to quantify mRNA levels, relative to uninjured controls, in spinal cords injured using a standard contusion model. Our results revealed a marked loss of neuron-specific mRNAs at the injury site. The surviving cells showed a characteristic inflammatory response that started at the injury site and spread to the distal cord. Changes in several mRNA levels were associated with putative regenerative responses in the spinal cord. Notably, phosphodiesterase 4, nestin, glia-derived neurite promoting factor, and GAP-43 mRNAs increased significantly. Other mRNAs clustered temporally and spatially with these regeneration-associated genes. Thus we have described global patterns of gene expression following acute SCI, and we have identified targets for future study and possible therapeutic intervention.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed2006

4Pi microscopy deconvolution with a variable point-spread function.

To remove the axial sidelobes from 4Pi images, deconvolution forms an integral part of 4Pi microscopy. As a result of its high axial resolution, the 4Pi point spread function (PSF) is particularly susceptible to imperfect optical conditions within the sample. This is typically observed as a shift in the position of the maxima under the PSF envelope. A significantly varying phase shift renders deconvolution procedures based on a spatially invariant PSF essentially useless. We present a technique for computing the forward transformation in the case of a varying phase at a computational expense of the same order of magnitude as that of the shift invariant case, a method for the estimation of PSF phase from an acquired image, and a deconvolution procedure built on these techniques.

Journal Article↗
PubMed2006

Physical structure of lakes constrains epidemics in Daphnia populations.

Parasites are integral parts of most ecosystems, yet attention has only recently focused on how community structure and abiotic factors impact host-parasite interactions. In lakes, both factors are influenced by habitat morphology. To investigate the role of habitat structure in mediating parasitism in the plankton, we quantified timing and prevalence of a common microparasite (Metschnikowia bicuspidata) in its host, Daphnia dentifera, in 18 lakes that vary in basin size and shape. Over three years, we found substantial spatial and temporal variation in the severity of epidemics. Although infection rates reached as high as 50% in some lakes, they did not occur in most lakes in most years. Host density, often considered to be a key determinant of disease spread, did not explain a significant amount of variation in the occurrence of epidemics. Furthermore, host resistance does not fully explain this parasite's distribution, since we easily infected hosts in the laboratory. Rather, basin shape predicted epidemics well; epidemics occurred only in lakes with steep-sided basins. In these lakes, the magnitude of epidemics varied with year. We suggest that biological (predation) and physical (turbulence) effects of basin shape interact with annual weather patterns to determine the regional distribution of this parasite.

Animal Diseases↗
PubMed2006

Processesof Tamarix invasion and floodplain development along the lower Green River, Utah.

Significant ecological, hydrologic, and geomorphic changes have occurred during the 20th century along many large floodplain rivers in the American Southwest. Native Populus forests have declined, while the exotic Eurasian shrub, Tamarix, has proliferated and now dominates most floodplain ecosystems. Photographs from late 19th and early 20th centuries illustrate wide river channels with largely bare in-channel landforms and shrubby higher channel margin floodplains. However, by the mid-20th century, floodplains supporting dense Tamarix stands had expanded, and river channels had narrowed. Along the lower Green River in eastern Utah, the causal mechanism of channel and floodplain changes remains ambiguous due to the confounding effects of climatically driven reductions in flood magnitude, river regulation by Flaming Gorge Dam, and Tamarix invasion. This study addressed whether Tamarix establishment and spread followed climate- or dam-induced reductions in annual peak flows or whether Tamarix was potentially a driver of floodplain changes. We aged 235 Tamarix and 57 Populus individuals, determined the hydrologic and geomorphic processes that controlled recruitment, identified the spatial relationships of germination sites within floodplain stratigraphic transects, and mapped woody riparian vegetation cohorts along three segments of the lower Green River. The oldest Tamarix established along several sampling reaches in 1938, and 1.50-2.25 m of alluvium has accreted above their germination surfaces. Nearly 90% of the Tamarix and Populus samples established during flood years that exceeded the 2.5-year recurrence interval. Recruitment was most common when large floods were followed by years with smaller peak flows. The majority of Tamarix establishment and Green River channel narrowing occurred long before river regulation by Flaming Gorge Dam. Tamarix initially colonized bare instream sand deposits (e.g., islands and bars), and most channel and floodplain changes followed the establishment of Tamarix. Our results suggest that Tamarix recruitment was triggered by large annual floods that were followed by years with lower peak flows, not by periods of low flow alone. Tamarix appears to have actively invaded floodplains, while Populus colonization has been limited. Thus, Tamarix invasion may have greatly influenced floodplain development and riparian vegetation composition along the lower Green River since the early 20th century.

Disasters↗
PubMed1986

Three-dimensional architecture of the Golgi complex observed by high resolution scanning electron microscopy.

In spite of many light and transmission electron microscopic studies of the Golgi complex, many important points remain unsettled. Recently, we have devised a method of observing the three-dimensional configurations of intracellular structures by scanning electron microscopy. We have used this method to study the three-dimensional structure of the Golgi complex in several tissues of the rat, paying special attention to three problems: the overall configuration; the spatial construction of each stack, and the relationship to neighbouring organelles. The interconnected stacks which form the Golgi complex are arranged in a manner characteristic of the particular cell type. In rat extraorbital lacrimal gland the stacks form an irregular network spread throughout the cell, while in anterior pituitary cells they form a hollow sphere. Continuities were seen between cisternae within a stack, and in one case a stack actually consisted of one helically wound cisterna. We also observed slender tubules connecting Golgi stacks to neighbouring rough endoplasmic reticulum. Vesicles, presumably transport vesicles, were frequently observed on the surface of the cisternae, most commonly at the cisternal rims. However the continuities among stacks and connections with cisternae and with the rough endoplasmic reticulum demonstrated here offer alternative routes for movement of substances through the cellular endomembrane system.

Animals↗
PubMed2002

Covert attention increases spatial resolution with or without masks: support for signal enhancement.

Visual attention can increase spatial resolution even when it leads to a decrease in performance. Whether this effect is mediated by reduction of external noise or by signal enhancement is an unsettled question. Although we previously demonstrated that attention can improve speed and accuracy in an acuity task, those experiments made use of a local postmask, which could be considered a source of external noise. In this work, a peripheral cue improved observers' abilities to indicate which side of a Landolt-square target had a gap whether or not a local postmask was used and with both central- and spread-neutral cues. In addition, we documented the presence of visual field inhomogeneities in a resolution task. Given that these experiments presented the target alone with no external noise added (i.e., without distracters or masks), our results indicate that transient attention enhanced the quality of the stimulus representation. Furthermore, because performance in the Landolt-square task indexes resolution, this attentional benefit indicates that transient attention can produce signal enhancement through finer spatial resolution.

Attention↗
PubMed2003

Cyclooxygenase-2 mRNA expression is associated with c-fos mRNA expression and transient water ADC reduction detected with diffusion MRI during acute focal ischemia in rats.

Cyclooxygenase-2 (COX-2) plays an important role in the development of injury during cerebral ischemia and inhibition of its activity can reduce infarct size. COX-2 expression during acute ischemia is caused by activation of post-synaptic glutamate receptors, which occurs during spreading depression and ischemic depolarization. Both of these phenomena cause a reduction in the apparent diffusion coefficient of water (ADC), which can be detected with diffusion-weighted magnetic resonance imaging. The reduction is believed to be caused by cellular swelling that occurs as cells depolarize. The goal of this work was to determine the spatial relationship between cyclooxygenase-2 mRNA (cox-2) expression, c-fos mRNA expression and ADC reduction during acute focal cerebral ischemia. Adult rats were subjected to either 30- or 60-min permanent occlusion of the middle cerebral artery. A 2-Tesla scanner was used to acquire diffusion-weighted echo-planar images throughout the ischemic period, which were used to calculate ADC maps. Cox-2 and c-fos mRNA were detected with (35)S in situ hybridization. The results indicate that, for rats subjected to 60-min ischemia, cox-2 was observed in superficial layers of cortex, where transient ADC reduction and c-fos expression were observed. The same was true for most rats subjected to 30-min ischemia. However, in a small number of rats of the 30-min group, cox-2 mRNA expression was observed in regions exhibiting transient and persistent ADC reduction with no c-fos expression. The results suggest that cox-2 mRNA expression during acute MCA occlusion is caused by either or both spreading depression and transient ischemic depolarization.

Acute Disease↗