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At least 1,297 records · Page 72Linked to original sources
PubMed1996

[Calcification of the pectineal ligament. An usual radiological imaging in pediatrics].

OBJECTIVES: Since its first description several years ago in the elderly subject, calcification of the Cooper ligament is often considered as a rare anecdotal phenomenon. We present the radiographic presentation which is often not well known. METHODS: Two experienced radiologists examined independently a series of 100 consecutive X-rays of the pelvis performed in a population of elderly subjects (mean age 84.4 +/- 9.2), looking for calcification of the Cooper ligament. RESULTS: Calcification of the Cooper ligament was observed on 7 of the 100 images of the pelvis. There was no significant difference in age, sex, or reason for ordering the examination between subjects with calcification and those without calcifications. Arterial calcifications were observed at an equal frequency. The usual aspect of the Cooper ligament was a fine opaque line following along the upper border of the iliopectinate crest. Oblique views visualized the calcification better. The line of calcification was either continuous of fragmented and was bilateral in all 7 cases. CONCLUSION: These images are apparently of no pathological significance. Clinicians should however be aware of the radiographic presentation in order to avoid confusion with visceral calcification or periosteal deposits.

Aged↗
PubMed1997

Promoter sequences of two homologous pectin esterase genes from Chinese cabbage (Brassica campestris L. ssp. pekinensis) and pollen-specific expression of the GUS gene driven by a promoter in tobacco plants.

The promoter regions of two genomic clones, GBAN215-6 and GBAN215-12 from Chinese cabbage (Brassica campestris L. ssp. pekinensis), were sequenced. The nucleotide sequences of their promoter regions were compared with that of the Bp19 pollen-specific gene of Brassca napus. High nucleotide sequence homologies were observed among these three genes in the region between 210 bp upstream and the putative transcription start site. A sequence motif TGTGGTG, which is similar to that of the PB core motif (TGTGGTT) of two tomato pollen-specific genes, LAT52 and LAT56, was present in these two cloned genes. To determine regulatory sequences responsible for the anther-specific expression of the gene BAN215-6, two recombinant plasmids, pBPE3 (-274- + 109) and pBPE4 (-816- + 109) containing different lengths of the promoter fused with the GUS gene, were constructed and introduced into tobacco plants by Agrobacterium-mediated transformation. The result showed that the 383 bp (-274- + 109) of the BAN215-6 promoter region was sufficient for the anther-specific expression of the GUS gene. The GUS expression in a tobacco plants transformed with these constructs was first detected in uninucleate microspores and persisted at in vitro germinated pollen tubes. The expression level was increased during anther development, reaching the highest level in mature pollens.

Brassica↗
PubMed1998

Pollen ablation of transgenic tobacco plants by expression of the diphtheria toxin A-chain gene under the control of a putative pectin esterase promoter from Chinese cabbage.

We previously showed that a 383 bp (-274 to approximately +109) promoter of a pollen-specific gene, GBAN215-6, had a property of a late gene in pollen development in transgenic tobacco plants. It drove GUS gene expression from uninucleate microspores to pollen tube growth of trinucleated cells. To more precisely characterize the specificity of the promoter, we placed the diphtheria toxin A-chain (DTx-A) coding region under the control of the GBAN215-6 promoter. Transgenic tobacco plants containing the GBAN215-6/DTx-1 were phenotypically normal until an early stage of flowering. The dehisced anthers do not contain pollen grains and the filament length of stamen was shorter than that of normal plants. Microscopic examination showed that ablation of pollen by the expression of DTx-A was variable. The transgenic tobacco plants containing one copy of the DTx-A gene show 50% aborted and 50% normal pollen, which suggests that this gene acts gametophytically. However, most of the transgenic plants with high copy number were male-sterile. When these male-sterile tobacco plants were backcrossed as female with pollen from wild-type tobacco plants, the fruit capsule sizes and seed yields of the next generation (BC1 lines) were severely reduced and the segregation of male-sterile to fertile plants in BC1 seeds was not Mendelian.

Artificial Gene Fusion↗
PubMed1998

[Effect of marine pectin in complex with mineral water on blood antioxidant system in experimental hyperlipidemia].

Experimental investigations on impact of biological active additives zosterin to food in complex with carbonate mineral waters on system peroxidate oxidation of lipids-antioxidant protection at hyperlipidemia have been conducted. It has been established the corrected action of each tested remedy taken separately and in different combinations on process of peroxidation of lipids and antioxidant functions of organism.

Animals↗
PubMed1998

Influence of dietary fiber on DNA adduct formation in rat tissues.

The occurrence of DNA-adduct-like indigenous compounds (I-compounds) was examined in tissue samples of rats differing in their microbial state and diet with or without pectins of different degrees of esterification (DE). For 21 days groups of six germfree and ten conventional rats were each fed either pectin-free or with diets containing 7.5% of three differently esterified pectin preparations (pectin A: DE 92.6%; pectin B: DE 70.8%; pectin C: DE 34.5%). DNA was isolated from colonic mucosa, liver, lung, kidney and measured by the highly sensitive 32P postlabelling assay for DNA adduct analysis. In germfree animals I-compounds were detected in all tissue samples after feeding the low-esterified pectin C. Under the higher-esterified pectins, A and B, a weak adduct formation could be demonstrated only in the liver, but not in the colonic mucosa, lung, and kidney. In conventional animals DNA adducts were found in all samples of colonic mucosa with the highest intensity in the control group, followed by the low-esterified pectin C group, and a weak intensity under the higher-esterified pectins A and B. The experiments show a tendency to a higher number and intensity of spots in the germfree compared with the conventional rats, in rats with a pectin-free compared with the pectin diet, and under the low-esterified compared with the higher-esterified pectins.

Animals↗
PubMed2003

Changes in macroscopic viscosity do not affect the release of aroma aldehydes from a pectinaceous food model system of low sucrose content.

The effects of pectin and viscosity on the release of a systematic series of aldehydes (alkanals, methyl-alkanals, alkenals, and alkandienals) were studied in a food model system of low sucrose content (10% w/w). The viscosity was varied by adding different amounts of Ca(2+) (0, 13.5, and 27 mg/g pectin) to the model system of constant pectin concentration (0.4% w/w). Air-liquid partition coefficients, K (37 degrees C), of the aroma compounds were determined in aqueous and pectin-thickened solutions. Diffusivities of the aroma compounds in water and three pectin-thickened solutions were estimated from release rate constants that were obtained via timed collection of volatiles in the gas phase and quantifications by dynamic headspace-gas chromatography. The partition coefficients increased as the carbon chain increased within each homologous series. Overall, no significant difference was found between partition coefficients of aldehydes in water and in pectin solutions except for 2-methyl-propanal and butanal that showed higher K values when pectin was present. Furthermore, the diffusional properties of the model system with a constant pectin level (0.4% w/w) remained constant when the viscosity was increased from 0.001 to 150 Pa s. It was concluded that neither pectin nor alterations in macroscopic viscosity as such influenced the release of aldehydes from the pectin-thickened food model system.

Aldehydes↗