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At least 1,297 records · Page 72Linked to original sources
PubMed1978

Direct analysis for cholesterol in gallstones by use of differential scanning calorimetry transition phase.

A cholesterol transition phase at 37.5 degrees C has been shown by use of a Differential Scanning Calorimeter. The transition, reversible and reproducible, is useful for analytical purpose. We propose an analytical method for cholesterol in gallstones based on this transition. The method requires no intermediate reactions and cholesterol can be assayed directly in the powdered stone, with no other pretreatment or reagents. The analysis is highly specific, requires about 10 min, and is quite precise (CV about 1.5%) and accurate (about 2%).

Calorimetry, Differential Scanning↗
PubMed1984

[Metabolic effects of gliclazide in diabetes type II patients. Study with indirect calorimetry].

An attempt is made to determine the effect of treatment with gliclazide on the manner in which obese patients with noninsulin-dependent diabetes dispose of an oral glucose load. Six patients were studied on two occasions - once after one month of an isocaloric diet containing 40% carbohydrate, and two months after gliclazide had been added. Body weight remained constant throughout the three months. However, fasting plasma glucose concentration fell and mean (+/- SEM) incremental insulin response to oral glucose increased significantly (p less than 0.05) with drug therapy (51.1 +/- 14.6 microU/ml vs 17.1 +/- 1.5 microU/ml). Glucose oxidation significantly improved after gliclazide treatment (16.9 +/- 2.4 g/3 h vs 7.5 +/- 2.1 g/3 h, p less than 0.02) in parallel with the fall in plasma glucose and the increase in insulin response. In addition, glucose storage significantly increased after drug therapy (44.1 +/- 10.1 g/3 h vs 28.4 +/- 7.2 g/3 h, p less than 0.001). In contrast, lipid oxidation fell with gliclazide treatment (7.2 +/- 0.7 g/2 h vs 12.0 +/- 1.7 g/3 h, p less than 0.02). This effect may be explained by improvement of the antilipolytic effect of insulin. In conclusion, improvement in glucose oxidation (25%) and in glucose storage (55%), together with reduced lipid oxidation, was observed after two months of gliclazide therapy.

Blood Glucose↗
PubMed1993

Walking and running energy expenditure estimated by Caltrac and indirect calorimetry.

The purpose of this study was to examine the accuracy of the Caltrac personal activity computer during walking and running. Ten women and 10 men walked at speeds of 2-5 mph and ran at speeds of 4-8 mph on a horizontal treadmill. Two Caltrac monitors were attached over opposite hips: one programed to give caloric expenditure and the other to give Caltrac counts. Oxygen uptake was measured simultaneously. Significant correlations were found during walking between Caltrac estimated and actual energy expenditure (r = 0.91) and between activity counts and net exercise VO2.kg-1 (r = 0.87). However, the Caltrac significantly overestimated energy cost during horizontal walking at speeds above 2 mph. Although there was a significant correlation between Caltrac estimated and actual energy expenditure during running (r = 0.71), the correlation between Caltrac counts and net exercise VO2.kg-1 was not significant (r = 0.29). There was no significant increase in Caltrac kcal or counts with increased running speed between 5 and 8 mph. It is concluded that the Caltrac is a valid indicator of physical activity during walking but does not adequately discriminate between running speeds of 5-8 mph.

Calorimetry, Indirect↗