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[Cat-scratch disease. Presentation of 2 cases in a family with serologic follow-up and discussion of diagnostic methods].

Two cases are reported of cat scratch disease which we consider worth describing both because of their familial presentation (the involved patients were brothers) and the serologic follow-up performed after diagnosis, which showed significant increases in IgG antibody titers to Bartonella henselae in both patients, one on day 35 and the other on day 45 after diagnosis. Cat scratch disease is a rarely diagnosed condition in our environment and it is easily misdiagnosed with other regional lymphadenitis; hence, a serologic test revealing a seroconversion or a significant increase in serologic titers will be very useful, as well as the epidemiologic antecedent of cat exposure.

Adolescent↗

[Database on nucleotide sequences used as primers of microorganisms].

The data base (DB) "Primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. This DB includes data on primers for the laboratory diagnostics of 20 bacterial genera (Aerococcus, Aeromonas, Bartonella, Borrelia, Burkholderia, Chlamydia, Clostridium, Corynebacterium, Escherichia, Francisella, Helicobacter, Legionella, Listeria, Mycobacterium, Mycoplasma, Salmonella, Shigella, Staphylococcus, Vibrio, Yersinia) and 6 viral families (Arenaviridae, Flaviviridae, Hepadnaviridae, Herpesviridae, Picornaviridae, Retroviridae). DB contains data on 145 pairs of primers and 530 bibliographic sources. The retrospective depth of DB is 10 years (1987-1996), and it is replenished as new Russian and foreign documented sources of information arrive.

Bacteria↗

Rochalimaea species stimulate human endothelial cell proliferation and migration in vitro.

Rochalimaea henselae and R. quintana are associated clinically with proliferative neovascular lesions. The effect of Rochalimaea species on human umbilical vein endothelial cell (HUVEC) proliferation and migration was evaluated in vitro. Cocultivation of Rochalimaea organisms with HUVECs resulted in enhanced HUVEC proliferation. Fibroblast proliferation was unaffected by R. henselae. HUVECs were also stimulated to migrate by Rochalimaea. When R. henselae organisms were disrupted and subjected to centrifugation, the ability to enhance HUVEC proliferation and migration was localized to the particulate, noncytosolic fraction. Trypsin treatment of this fraction diminished its stimulatory activity. These data suggest that the neovascular manifestations of infection with Rochalimaea are likely caused by the production of an angiogenic factor by these bacteria.

Bartonella↗

Intermediate uveitis and retinal vasculitis as manifestations of cat scratch disease.

PURPOSE: To study the ocular manifestations of systemic Rochalimaea infection. METHODS: We examined a healthy 21-year old woman who had floaters in both eyes. A bilateral mild vitreitis and multiple foci of retinal vasculitis were found; during the ensuing two weeks, exudates appeared over the inferior pars plana. The patient owned five kittens but had no history of cat bites or scratches. Serum levels of antibodies to Rochalimaea were elevated. RESULT: The retinal vasculitis and vitreitis resolved after three weeks of therapy with ciprofloxacin hydrochloride without concomitant anti-inflammatory therapy. CONCLUSION: Rochalimaea infection should be considered in the differential diagnosis of intermediate uveitis and retinal vasculitis.

Adult↗

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Bartonella↗

[Bacillary angiomatosis].

Bacillary angiomatosis (BA) is a rare infectious disease usually associated with HIV infection. Recent molecular biologic investigations confirm that both Rochalimaea henselae and Rochalimaea quintana can cause BA. The bacteria can be identified by Warthin-Starry staining and electron microscopy. The typical clinical signs are solitary or multiple dermal or subcutaneous nodules. Bone, liver, spleen and other organs may also be involved. We describe the clinical and histological features of a 39-year-old HIV-infected patient with cutaneous and bony lesions of BA. All manifestations of BA disappeared during therapy with erythromycin.

AIDS-Related Opportunistic Infections↗