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Distal pancreatectomy with and without splenectomy.

Splenectomy is performed routinely during distal pancreatectomy, yet the spleen has an important role in host defence and can often be preserved. A personal series of 100 distal pancreatectomies undertaken for pancreatic disease between 1978 and 1990 included 23 patients undergoing total pancreatic resection. The remaining 77 patients, who form the basis of the present report, underwent primary distal pancreatectomy and comprised 34 women and 43 men with a median age of 41 years (range 17-78 years). Conventional distal pancreatectomy including splenectomy was performed in 42 patients (55 per cent) for chronic pancreatitis (34 patients), pancreatic neoplasia (six patients), suspected pancreatitis (one patient) or pancreatitic trauma (one patient). Conservative resection with splenic preservation was performed in 35 patients (45 per cent) for chronic pancreatitis (12 patients), suspected pancreatitis (13 patients, including eight patients with pancreas divisum), pancreatic neoplasia (six patients), recurrent acute pancreatitis (two patients) and pancreatic trauma (two patients). There were no postoperative deaths in either group. Early complications followed conventional resection in 10 patients (24 per cent) and conservative resection in seven patients (20 per cent). In five patients the splenic vessels were ligated away from the splenic hilum and the spleen was left in situ, but subsequent isotope scans and haematological indices showed no hyposplenism. The spleen can safely be preserved in many distal pancreatic resections, including those for inflammatory disease, and we now prefer a retrograde technique for dissecting the pancreas off the splenic vessels.

Adolescent↗

Subtotal splenectomy in Gaucher's disease: towards a definition of critical splenic mass.

Partial splenectomy is currently the favoured surgical approach for Gaucher's disease. Preservation of splenic tissue is indicated to prevent susceptibility to overwhelming postsplenectomy sepsis, to delay the massive deposition of glucocerebroside in the liver and bones, and to relieve symptoms of hypersplenism. Controversial issues and technical problems related to partial splenectomy for Gaucher's disease are discussed and a definition of critical splenic mass considered.

Gaucher Disease↗

The protection of small amounts of splenic tissue against intravenous pneumococcal challenge following subtotal splenectomy.

The asplenic state increases susceptibility to overwhelming bacterial infection. We studied the ability of small amounts of splenic tissue to protect against intravenous pneumococcal challenge following subtotal splenectomy in which approximately 75% of the spleen was removed. Animals that had undergone subtotal splenectomy were more resistant to challenge than were asplenic animals, but were not as resistant as were control animals. The small amount of residual splenic tissue confers some degree of protection against intravenous bacterial challenge.

Animals↗

Heart and spleen twin grafts in rats. II. Delayed host splenectomy.

Simultaneous auxiliary transplantation of the spleen has been shown to delay and attenuate the rejection of cardiac grafts in rats. In the experiments reported here, 144 such twin grafts in Lewis and ACI rats were explored in an attempt to determine whether removal of the host's spleen would further facilitate graft survival in this model. Thus, 87 rats were submitted to host splenectomy at various time intervals after implantation of the twin grafts. After considerable technical problems were overcome, it was found that this additional maneuver induced permanent (greater than 5 months) survival in 64% of Lewis-to-ACI twin grafts. The important requirement for this success was timing; delaying host splenectomy for 3 to 5 days after implantation of the twin graft was mandatory.

Animals↗

The incidence of post-splenectomy sepsis and herpes zoster in children and adolescents with Hodgkin disease.

The occurrence of sepsis due to Streptococcus pneumoniae and Hemophilus influenza and of herpes zoster (HZ) was reviewed in a series of 72 consecutive, previously untreated children and adolescents with Hodgkin disease. There was not a statistically significant difference in the risk of developing sepsis within five years of diagnosis between patients who had (16.6%) or had not (6.2%) undergone splenectomy. Sepsis occurred most frequently among patients treated initially with total nodal irradiation and combination chemotherapy. The estimated risk of HZ during the first five years after diagnosis was 34%. Patients treated initially with irradiation and combination chemotherapy had a significantly greater risk of developing HZ than patients treated initially with only irradiation (P less than 0.05). Although trends were present which suggested that splenectomy and the extent of disease at diagnosis may influence the occurrence of HZ, these did not achieve statistical significance. Survival was not influenced by the occurrence of HZ.

Adolescent↗

Plasmodium yoelii: splenectomy alters the antibody responses of infected mice.

The antibody response to Plasmodium yoelii is altered in splenectomized mice. Sera were obtained from sham-operated or splenectomized DBA/2 and C57BL/6 mice on Days 11, 18, and 24 after infection with nonlethal P. yoelii 17x and used to precipitate metabolically radiolabeled parasite antigens. Mice of both strains responded to many antigens. However, only splenectomized DBA/2 mice made strong antibody responses to antigens of approximately 110, 56, 50, 40, 35, and 20 kDa. Metabolically radiolabeled parasite extracts prepared in sham-operated and splenectomized mice appeared identical on SDS-PAGE. Thus it is unlikely that expression of new parasite antigens in splenectomized DBA/2 mice accounts for these results. Parasite-reactive IgM and IgG antibody responses were also modulated by splenectomy. Levels of IgM increased in splenectomized DBA/2 mice and decreased in C57BL/6 mice. Both mouse strains had slight to moderate increases in IgG when infected after splenectomy. The results suggest that when the spleen is present, responses to specific antigens are markedly suppressed. Alternatively, it is possible that in the absence of a spleen, antigen processing and presentation occurs in other tissues such as the lymph nodes or liver, leading to responses that are qualitatively different than those which occur when the spleen is present.

Animals↗

Splenectomy predisposes to fungal sepsis through defective phagosome formation.

Postsplenectomy septic sequelae may be fatal, but the mechanisms are unclear. We hypothesized that peritoneal macrophage (PM phi) antimicrobial function is abnormal following splenectomy and that this may predispose to increased mortality from the fungal pathogen Candida albicans. Study 1 (in vivo): female CD-1 mice were randomized into control (C), laparotomy (L), or laparotomy+splenectomy (L + S) and inoculated with C. albicans (10(7) organisms, ip) and were studied for mortality. Study 2 (in vitro): mice were randomized to C, L, or L + S groups. Twenty-four hours later, PM phi were harvested and studied for their antifungal activity, including percentage PM phi ingestion of C. albicans and vacuolar sealing of C. albicans within PM phi, percentage C. albicans killing, and superoxide anion (O2-) generation, the mechanism by which candida are killed. Results showed decreased phagocytosis and killing of C. albicans in the L + S group (P < 0.05 vs C) and reduced vacuolar sealing (P < 0.05 vs C) but significantly higher O2- release compared to that in other groups (P < 0.05). Mortality in the L + S group from C. albicans sepsis was significantly higher than that in the other groups (60% compared to 20% in the L group and 13% in C, P < 0.02). This may have resulted from L + S-induced defective phagocytosis of C. albicans and depressed C. albicans killing but increased O2- release in response to candida. This discrepancy between decreased killing and increased O2- may result from increased leakage of O2- from more unsealed vacuoles in the L + S group. Thus, L + S may predispose to candida-induced mortality through defective PM phi intracellular candida killing while enhancing the release of O2- extracellularly from unsealed vacuoles, causing tissue injury.

Animals↗

Laparoscopic splenectomy in children. Preliminary results and comparison with the open technique.

To determine the safety and efficacy of laparoscopic splenectomy (LS) in children, a retrospective review of our preliminary experience using LS was compared to results in patients who previously underwent open splenectomy (OS). From July 1993 to January 1995, we performed eight LS procedures in six children with hereditary spherocytosis (HS) and two with immune thrombocytopenic purpura (ITP). Laparoscopic cholecystectomy was simultaneously done in one case with HS. There were 4 males and 4 females who ranged in age from 5 to 15 years--an average age of 8.8 years. Two cases in the early series required a counterincision because of bleeding. Eleven patients who previously underwent OS in our department were used to compare demographics, operative courses, and surgical outcomes. The ages, genders, diseases, body weights, and spleen weights were comparable between LS group and OS groups. The operative time for the LS group was statistically longer than for the OS group (226 +/- 24 min vs 101 +/- 8 min, P < 0.001). The estimated blood loss in the LS group was similar to that of the OS group (100 +/- 39 ml vs 73 +/- 11 ml. P = 0.97). There were no peri- or postoperative complications in two groups. The postoperative hospital stay of LS group was statistically shorter than that of the OS (6.8 +/- 0.6 days vs 10.4 +/- .05 days, P < 0.0001). LS provided better cosmesis and minimized trauma in children over OS. LS appears to be a safe and effective procedure in children, and is useful in the management of pediatric patients with HS or ITP.

Adolescent↗

Portal vein thrombosis after splenectomy successfully treated by an enormous dosage of fibrinolytic agent in a short period: report of two cases.

Portal vein thrombosis (PVT) after splenectomy in a patient with portal hypertension occurs with unusually high frequency. Recently, two patients with PVT following splenectomy were treated by fibrinolytic therapy with an enormous dosage of urokinase (UK) in a short period. PVT was quickly dissolved without side effects and the patients are now doing well without any recurrence of PVT. Therefore, when there is no evidence of bowel infarction, fibrinolytic therapy with an enormous dosage of UK over a short period is deemed to be both effective and essential as a conservative therapy for PVT.

Adult↗

Impaired protein synthesis in leukemic hairy cells induced by splenectomy.

A case of hairy-cell leukemia in which protein synthesis by the leukemic hairy cells could be followed-up before and after splenectomy is presented. After splenectomy a remarkable reduction of initially enhanced protein synthesis was found. This observation supports the view that the splenic microenvironment has an important influence on the proliferative activity of hairy cells.

Female↗

Transitory IgA-deficiency, persistent IgE deficiency and recurrent respiratory tract infectious disease after splenectomy.

In a 14 year old boy, a ruptured spleen was removed. Two months after the splenectomy he developed recurrent infections of the respiratory tract. A deficiency of IgA and IgE in his blood serum was found. His impaired resistance to respiratory tract infections responded well to substitution therapy with IgA. Spontaneous clinical recovery occurred during 3 years after the splenectomy, and was parallelled by a slow return of IgA to normal levels, whereas the absence of IgE persisted. The identical twin brother of the patient, who had his spleen intact, had normal levels of serum IgA and did not suffer from frequent respiratory tract inflammations.

Adolescent↗

Chronic relapsing experimental allergic neuritis in Lewis rats: effects of thymectomy and splenectomy.

Experimental allergic neuritis (EAN) was induced in Lewis rats aged 4 months by the inoculation of whole bovine dorsal root with Freund's complete adjuvant. Prolonged follow-up demonstrated that a relapsing course is a regular feature of the disorder in animals at this age. Although the initial disease episode was the most severe, clinical recovery from subsequent relapses was less satisfactory, this probably being related to persistent morphological abnormalities in the peripheral nervous system. Antecedent thymectomy, splenectomy, or the two combined, had little effect on the clinical course of the disorder, apart from reducing the duration of relapses. This was only statistically significant following combined thymectomy/splenectomy. Histological abnormalities, however, tended to be less severe in the operated as compared with normal control or sham-operated animals with EAN. The animals must have attained an immunocompetent state at the time of thymectomy and/or splenectomy. The capacity to develop EAN presumably resides in the draining lymph nodes and the occurrence of relapses is due to the continuing presence of antigen at the injection sites.

Animals↗

IgA nephropathy and idiopathic thrombocytopenic purpura with splenectomy: a case report.

A 14-year-old boy who had had a splenectomy at the age of 2 years for idiopathic thrombocytopenic purpura, suffered from IgA nephropathy. Serum IgA and IgE levels were elevated and low levels of circulating immune complexes were detected. Splenectomy may play a role in the pathogenesis or susceptibility to IgA nephropathy by means of decreased clearance of circulating immune complexes or impaired immune regulation, such as increased IgA synthesis.

Adolescent↗

Immunologic studies before and after splenectomy in a patient with the Wiskott-Aldrich syndrome.

Sequential studies of cellular and humoral immunity were conducted in an infant with the Wiskott-Aldrich syndrome prior to and after a splenectomy for uncontrollable hemorrhage. All measures of cellular immunity showed gradual improvement during the 8-month period after surgery. Serum isohemagglutinins, diphtheria and tetanus antibodies, and the percentage of immunoglobulin-bearing B cells did not change significantly from presplenectomy values. The serum IgE concentration declined from a high of 10,800 IU/ml at 1 month postsplenectomy to a low of 860 IU/ml at 5 months after surgery and the IgG concentration gradually decreased from a high of 1880 mg/dl presplenectomy to a low of 620 mg/dl 8 months later. The platelet count ranged from 64,000 to 206,000/mm3 for the first 6 months after splenectomy. It decreased precipitously 6.5 months after the operation; at the same time there was a marked rise in platelet-bound IgG antibody (PB-IgG). The PB-IgG declined rapidly following vincristine therapy and, after another rise, declined more gradually following steroid therapy.

Antibody Formation↗

[Splenectomy: surgical indications in malignant hematologic diseases].

Indications for splenectomy in malignant hematological diseases are discussed for relief of symptoms (splenomegaly, cytopenias), for staging (M. Hodgkin) or improvement of survival (hairy cell leukemia). The role of splenectomies is also evaluated for Non-Hodgkin lymphomas, chronic lymphatic leukemia, myelofibrosis and for chronic myeloic leukemia.

Hematologic Diseases↗

[Radiologic alternatives of splenectomy].

Partial peripheral splenic embolization can be performed in case of incurable thrombocytopenia due to hypersplenism without following splenectomy. A combined proximal and peripheral splenic embolization should only be carried out immediately prior to splenectomy if complications of hemorrhage due to extreme splenomegaly are to be expected. An only proximally performed occlusion can be by-bassed by collaterals. Anatomic blood vessel variations, techniques and complications are discussed in 6 cases.

Combined Modality Therapy↗

Effects of splenectomy on production of poly I:C induced serum interferon in rabbits.

Splenectomy in rabbits causes a temporary reduction in interferon responding capacity of Poly I:C, followed by a period of hyperreactivity. At the same time a fraction of interferon with slightly different physiocochemical properties emerges. This could indicate that the spleen plays a relatively important role in the interferon response to Poly I:C. Splenectomy could be followed by the development of a compensatory cell population responsible for the production of interferon with slightly different molecular weight, isoelectric point and stability in urea.

Animals↗

Pseudocyst following splenectomy: impact of CT and ultrasound on its diagnosis and management.

Three cases of pseudocysts occurring after splenectomy are presented. Sonography and computed tomography were helpful in diagnosing the pseudocysts as well as providing guidance for subsequent drainage. Demonstration of a cystic mass adjacent to the tail of the pancreas following splenectomy suggests the diagnosis of postsplenectomy pseudocyst and has important implications for clinical management.

Adult↗