[Isolation and characterization of hemagglutinin from the stock culture of Actinomyces streptomycini str. 32-12].
From the culture fluid of Actinomyces streptomycini streptomycin was extracted and then hemagglutinin, a biologically active substance, was isolated. The isolation included ethanol fractionation, sulfate ammonium protein precipitation, elimination of contaminating components via centrifugation, dialysis and subsequent lyophilization. The resultant hemagglutinin showed activity towards animal and human erythrocytes, and blast-transforming and mitogenic activity towards human lymphocytes.