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PubMed1989

[Changes in primary heat sensitivity of rhabdomyosarcoma PA-2 cells in rats during selection for heat resistance].

Using the technique of multistep selection, a thermoresistant substrain (TR RA-2T45) was obtained from a rhabdomyosarcoma RA-2 strain. The cells of this substrain retain the ability to form lung metastasis after a 20 minute treatment at 45 degrees C in vitro. The selection of cells for their ability to retain their reproduction activity is accompanied by the increase in their primary thermoresistance. The increased reproductive thermoresistance and primary thermoresistance of the selected cells is hereditary stabilized. Cells of both original strain and its substrain are capable of thermal tolerance, which makes perspective the selection for further increase in thermoresistance in RA-2T4t cell population.

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