Molecular structure of crystalline Streptomyces griseus protease A at 2.8 A resolution. I. Crystallization, data collection and structural analysis.
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Feeding difficulties and growth failure are common problems in the premature infant who has been in a Neonatal Intensive Care Unit. This article, the fifth in a series, presents growth parameters and growth expectations for the premature infant. It also discusses the pathophysiology, causes, and assessments necessary to determine growth failure in these infants.
Energy-dispersive X-ray microanalysis (EDS) was compared in fresh- and frozen bulk hydrated tissues using the Environmental SEM (ESEM) and conventional cryo-SEM, respectively. Analysis of globoid inclusions of Eucalyptus calophylla seed from two soil types demonstrated that higher levels of cations (K, Ca, Mg, Al, Fe, Mn, Cu, Zn) occurred in seeds from soils containing higher levels of Al, while EDS-detectable levels of S and P were dependent upon the techniques utilised. Cumulative changes in ESEM-EDS-detectable levels of S and P were characterized by collecting cumulative spectra from nutrient standards and compared with those for K. Progressive increases in K occurred and were consistent with an enriching effect. Levels of S and P increased during early analysis (40-60 sec live time) and decreased thereafter. The semi-conductive nature of biological samples, the loss of anions and gain of cations from the net negatively-charged electron interaction volume contributed to an electrochemical bias. These local modifications in fluid chemistry were reversible. Dehydration effects also occurred in stable, 'wet' samples. These differences indicated that EDS in ESEM may be limited to cations rather than anions, and that changes in fluid electrochemistry and dehydration may affect the level and distribution of elements.
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Within surgical departments, a large amount of antibiotics is used for perioperative prophylaxis. Despite the existence of several guidelines and recommendations for administering antibiotic prophylaxis, mistakes still do occur and have an unknown impact on outcome severity. Based on the electronic anaesthesia records of 4304 patients undergoing defined surgical procedures requiring perioperative antibiotic prophylaxis, a matched pairs approach was used to evaluate the impact of inadequate antibiotic prophylaxis on hospital mortality and prolonged length of stay on intensive care. Stepwise regression models were developed to predict the impact of inadequate antibiotic prophylaxis on outcome measures. An inadequate antibiotic prophylaxis was found for a total of 877 cases. 77.9% of cases were successfully matched, leading to 683 cases and controls each. The crude mortality ratio of cases to controls was 1.5 (cases = 21 versus controls = 14; P = 0.19). The case group had a significantly (P < 0.01) prolonged stay on ICU when analysed as a metric variable. Using logistic regression analysis, we could determine that inadequate antibiotic prophylaxis had no impact on either hospital mortality or prolonged length of stay on ICU (>1 day; yes or no).
There were 574 scrapie positive suspects (histopathological scrapie lesions present) and 198 scrapie negative suspects (histopathological scrapie lesions absent). The greatest number of scrapie cases were recorded in sheep of 2, 3 and 4 years of age which represented 17%, 36% and 23% of the scrapie positive suspects, respectively. The sign sensitivities and specificities for the ten recorded signs were, respectively: pruritus (62%, 42%), ataxia (23%, 74%), hyperaesthesia (32%, 74%), wool loss (25%, 73%), fleece discolouration (29%, 85%), bruxism (23%, 69%), nibbling reflex (17%, 58%), head rubbing (47%, 78%), poll rubbing (25%, 83%). These single signs had poor discriminatory values with likelihood ratios close to one (range 0.89-1.21); combinations of the four signs, pruritus, wool loss, ataxia, hyperaesthesia and emaciation were more discriminatory (range 0.30-4.3). This study covered a time period when bovine spongiform encephalopathy (BSE) might have been introduced into the sheep population on the Shetland Islands via contaminated feed. No temporal changes could be detected in the age structure of the affected animals.
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In 1998, the Air & Surface Transport Nurses Association ASTNA (formerly known as the National Flight Nurses Association [NFNA]) sponsored a follow-up safety survey to one it had conducted in 1988. The survey was sent out to all association members and identified whether a respondent of the survey was employed by a program that had policies addressing the safety topics identified in the survey and were compliant with the topic. Covered topics included scheduling practices, rest, refusal to fly because of safety issues, safety briefings, safety training, chemical restraints, hot off-loading, personal protective gear, and safety equipment installed in aircraft. The 1998 NFNA safety survey replicated a safety survey sent in 1988. The results of the two surveys were compared using side-by-side analysis with graphic comparison of trends that have occurred during the 10-year period. With the exception of hot loading/unloading, every category of questions recognized an increased frequency of both written policies and compliance or practice. The most significant improvement in compliance was "personal protective gear;" the category engendering the most concern was "refusal to fly due to safety issues." In 1998, only 52% of respondents worked in programs that "defined the flight nurses' responsibility and acceptable reasons for refusal to participate."
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