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[The treatment of Actinomyces pyogenes endometritis by intrauterine Gentaseptin administration].

10 cows suffering from bacteriological proved A. pyogenes-endometritis have been treated by a single intrauterine infusion of GENTASEPTIN. All animals were cured. Clinical findings were confirmed by bacteriological tests. All animals conceived at the first insemination after treatment. The intrauterine application of GENTASEPTIN seems to be a very efficient treatment of A. pyogenes-endometritis as compared to hitherto existing treatments.

Actinomyces↗

[Stability and specificity of extracellular protein inhibitor for trypsin from Actinomyces janthinus 118].

Some properties of protein inhibitor for trypsin (TI) from Act. janthinus 118 were studied. It was shown that TI has an antitrypsin activity within a wide pH range with a maximum at about 9,5. At 4 degrees and 20 degrees C TI is stable for 24 hours within the pH range of 6,0--11,0. At 100 degrees C TI is more stable in the slightly acid region of pH than at neutral or alkaline conditions. Trypsin and chymotrypsin inactivate the inhibitor for 8 hours. TI inhibits trypsin, fibrinolysin, subtilisin, pronase and terrilytin, but have no effect on chymotrypsin, thrombin, papain and pepsin. The dissociation constants for the trypsin-inhibitor complex were found to be 1,7.10-8 M, 4,1.10-9 M and 2,4.10-10 M, with casein, p-nitroanilide benzoylarginine and tosylarginine methyl ester used as substrates, respectively. The corresponding dissociation rate constants for the subtilisin-inhibitor complex were equal to 1.10-9 M and 4.10-10 M with casein and carbobenzoxy-L-alanyl-L-alanyl-L-leucin p-nitroanilide used as substrates, respectively.

Actinomyces↗