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PubMed1999

Model-independent analysis of the orientation of fluorescent probes with restricted mobility in muscle fibers.

The orientation of proteins in ordered biological samples can be investigated using steady-state polarized fluorescence from probes conjugated to the protein. A general limitation of this approach is that the probes typically exhibit rapid orientational motion ("wobble") with respect to the protein backbone. Here we present a method for characterizing the extent of this wobble and for removing its effects from the available information about the static orientational distribution of the probes. The analysis depends on four assumptions: 1) the probe wobble is fast compared with the nanosecond time scale of its excited-state decay; 2) the orientational distributions of the absorption and emission transition dipole moments are cylindrically symmetrical about a common axis c fixed in the protein; 3) protein motions are negligible during the excited-state decay; 4) the distribution of c is cylindrically symmetrical about the director of the experimental sample. In a muscle fiber, the director is the fiber axis, F. All of the information on the orientational order of the probe that is available from measurements of linearly polarized fluorescence is contained in five independent polarized fluorescence intensities measured with excitation and emission polarizers parallel or perpendicular to F and with the propagation axis of the detected fluorescence parallel or perpendicular to that of the excitation. The analysis then yields the average second-rank and fourth-rank order parameters ( and ) of the angular distribution of c relative to F, and and , the average second-rank order parameters of the angular distribution for wobble of the absorption and emission transition dipole moments relative to c. The method can also be applied to other cylindrically ordered systems such as oriented lipid bilayer membranes and to processes slower than fluorescence that may be observed using longer-lived optically excited states.

Animals↗
PubMed2001

Simulation analysis of muscle activity changes with altered body orientations during pedaling.

Testing hypotheses related to the effect of gravitational orientation on neural control mechanisms is difficult for most locomotor tasks, like walking, because body orientation with respect to gravity affects both sensorimotor control and task mechanics. To examine the mechanical effect of body orientation independently from changes in workload and posture, Brown et al. (J. Biomech. 29 p. 1349, 1996) studied pedaling at altered body orientations. They found that subjects pedaling at different orientations changed needlessly their muscle excitations, putatively to preserve body-upright pedaling kinematics. We tested the feasibility of this hypothesis using simulations based on a three biomechanical-function pair organization for control of lower limb muscles (limb extension/flexion pair, extension/flexion transition pair, and foot plantarflexion/dorsiflexion pair), where each pair consists of alternating agonistic/antagonistic muscles. Adjustment of only three parameters, one to scale the muscle excitations of each pair, was sufficient to preserve pedaling kinematics to altered body orientation. Because these adjustments produced changes in muscle excitation and net joint moments similar to those observed in pedaling subjects, the hypothesis is supported. Moreover, the effectiveness of a decoupled gain adjustment procedure where each parameter was adjusted by error in only one aspect of the pedaling trajectory during each iteration (i.e., cadence adjusted the Ext/Flex parameter; peak-to-peak variation in crank velocity over the cycle adjusted the transition parameter; average ankle angle over the cycle adjusted the foot parameter) further supports the distinct function of each muscle pair.

Bicycling↗
PubMed1997

Orientation of the trabecular pattern of the distal radius around the menopause.

The trabecular pattern depicted on radiographs of the distal radius of women around the menopause is analysed by a new method to measure orientation. It is shown that the trabecular pattern on radiographs of the distal radius is aligned preferentially along the axial direction and to a lesser extent along the transverse direction. Two minima of orientation are found at 60 degrees off the axial direction. A significant correlation is found between the average value of the orientation in the radius and the average lumbar bone mineral density. It is known that the bone mineral content decreases around the menopause. Previous reports on the present images describe several features that were found to be related to bone mineral content and to time. The orientation of the trabecular pattern, however, is found to be independent of time, at least during the two years of the investigation. Therefore, it is concluded that the orientation of the radiographic trabecular pattern tends to remain stable even if some features of the pattern evolve. Previous studies on radiographs of the radius of children and elderly women and men provide evidence for the tendency of orientation to remain stable not only in women around the menopause, but also in men and women during the entire adult life.

Analysis of Variance↗
PubMed2001

Micropattern orientation and spatial localization.

A current, popular, theory of spatial localization holds that the visual system represents the location of simple objects by a single positional tag, the accuracy of which is largely independent of the internal properties of the object. We have already presented evidence of the limitations of such a view (Keeble & Hess (1998). Vision Research, 38, 827-840) in that 3-micropattern alignment performance was found to be dependent on the orientation of the micropatterns. We tested whether this was caused by a local anisotropy in positional coding by conducting 3-micropattern bisection experiments with varying patch orientation. No corresponding effect of patch orientation was found, implying a difference in the mechanisms used for the two tasks. In a further experiment we show that alignment task performance is very similar to the otherwise identical 2-patch orientation discrimination task. We conclude that the 3-micropattern alignment task is mediated by orientational mechanisms. We therefore present a 2nd-order orientation model for 3-patch alignment.

Anisotropy↗
PubMed1998

Tuning of orientation detectors in human vision.

A new method to study the tuning of orientation detectors in the human visual system is proposed. The stimulus consists of a sequence of flashed sinusoidal gratings of random orientations and spatial phases shown at a fast presentation rate. The subject's task is to report, as fast as possible, when the presence of a particular orientation (horizontal, vertical, or oblique) is seen in the stimulus sequence by pressing a button. The data are analyzed by calculating the empirical distribution of orientations present in the stimulus sequence within an optimal time-window before the button was pressed. The resulting orientation distributions show a "Mexican hat" shape, which resembles the distributions obtained in some single neurons of monkey primary visual cortex using a similar method (Ringach et al., 1997). The findings are consistent with the idea of "lateral inhibition" between neighboring detectors in the orientation domain.

Female↗
PubMed1998

Integration of local orientation in strabismic amblyopia.

In order to investigate the processes which integrate local orientation information in observers with strabismic amblyopia, we measured contrast thresholds for discriminating the global orientation of a pattern (3 "bars") comprised of Gabor patches. We found that in both eyes of amblyopic observers, as has been reported for normal observers [Saarinen, Levi & Shen (1997) Proceedings of the National Academy of Sciences USA, 94, 8267-8271], there is an approximately two-fold enhancement of contrast sensitivity when the global and local orientations are aligned (relative to mixed orientations), and a smaller enhancement when the global and local orientations are orthogonal. This orientation dependent enhancement occurs despite substantial losses of contrast sensitivity. These results suggest that the integration processes in the amblyopic eye that operate to enhance detection are essentially intact.

Adult↗
PubMed1999

Orientation-based texture segmentation in strabismic amblyopia.

Texture segmentation of 'target' Gabors from an array of 'background' Gabors was measured in terms of the difference in orientation between the two regions, as well as the difference in orientation within each region. Segmentation was shown to occur on the basis of local orientation differences at the boundary between the target and background regions (Nothdurft, H.C. (1992). Feature analysis and the role of similarity in preattentive vision. Perception and Psychophysics, 52, 355-375.). We obtained similar results for both the amblyopic and non-amblyopic eye of three strabismic amblyopes, and showed also that the effects of texture undersampling and positional jitter were similar for the two eyes. This pattern of results is consistent with intact mechanisms of texture perception in amblyopic cortex, and suggests also that any amblyopic deficits in first-order cortical units (undersampling and/or positional uncertainty) do not limit higher-order texture segmentation processes. Therefore, first- and second-order processes involved in perceptual grouping of oriented elements (that appear to be abnormal in amblyopic cortex; Kovács, I., Polat, U., Norcia, A.M. (1996). Breakdown of binding mechanisms in amblyopia. Association for Research in Vision and Ophthalmology Abstracts; Mussap, A.J., Levi, D.M. (1995). Amblyopic deficits in perception of second-order orientation. Investigative Ophthalmology and Visual Science (Supplement), 36, S634; Mussap, A.J., Levi, D.M. (1998). Amblyopic deficits in perceptual grouping. Vision Research, submitted) do not contribute to texture perception based on orientation contrast.

Adult↗
PubMed1999

Which end is up? Two representations of orientation in visual search.

What is the orientation of an object? A simple line has an axis of orientation. That line, turned upside-down, is indistinguishable from the original line. Thus, the possible orientations of a line range from 0 to 180 degrees. Most objects, however, have an axis and a polarity. A polar object, turned upside-down, looks upside-down. Accordingly, the orientations of a polar object range from 0 to 360 degrees. A series of visual search experiments were run to determine if preattentive processes represent orientation in a 180 or a 360 degrees framework. Results suggest that preattentive orientation is represented in 180 degrees. Experiments 1 and 4 show that search for a target rotated 90 degrees from the distractors is more efficient than search for a target rotated 180 degrees from the distractors. Experiments 2, 3, and 5 use a variety of different stimuli to demonstrate that search for targets rotated 180 degrees from distractors is inefficient.

Attention↗
PubMed1999

Orientation tuning of the transient-stereopsis system.

Stereo-perception appears to be mediated by at least two systems: a transient system that processes stimuli presented briefly and a sustained one that processes stimuli presented for longer durations. In this paper we investigated the tuning of the transient-stereopsis system to stimulus orientation. Narrowband-gabor targets with a constant envelope size (Gaussian standard deviation of 1 degree) were presented for brief (140 ms) durations at large (from 4 to 8 degrees) disparities. The results were as follows: (1) while observers could extract depth from orthogonally-oriented gabors at above chance levels, their performance was worse than that with gabors of matched orientation; (2) varying the relative contrasts of the two orthogonally oriented gabors of the same spatial frequency resulted in a reduction in performance; (3) varying the relative spatial frequencies of the orthogonally-oriented gabors impaired performance, relative to that for matched frequencies; and (4) varying the relative contrasts of orthogonal gabors that were at different spatial frequencies could improve performance. These results indicate that transient stereo-performance in the orthogonal condition was not mediated by the channels that extracted depth in either the horizontal- or vertically-matched gabor conditions. This apparent lack of orientation tuning is indicative of a second-order pathway. That this performance was mediated by a binocular, as opposed to a monocular channel, is supported by the finding that performance decreased as the contrast of one of the gabors was reduced. The finding that performance with orthogonal gabors of unmatched spatial frequency (0.5 and 4 cpd) could be improved by varying their relative contrasts suggests that the binocular spatial-frequency tuning exhibited by this channel is broadband in nature. Finally, the observation that lowering the contrast of either the high or low spatial-frequency gabor improved performance suggests the presence of at least two broadband channels: one with its peak sensitivity at a low and the other at a high spatial-frequency.

Contrast Sensitivity↗
PubMed1999

The stereoscopic (cyclopean) motion aftereffect is selective for spatial frequency and orientation of disparity modulation.

Across two experiments, this study investigated the spatial frequency tuning and orientation tuning (both in the disparity domain) of the stereoscopic (cyclopean) motion aftereffect. In Experiment 1, observers adapted to a moving stereoscopic grating of a given cyclopean spatial frequency and tested for the motion aftereffect with a static grating of the same or different spatial frequency. Robust motion aftereffects were induced only when the spatial frequency of the adapt and test stimuli was the same. In Experiment 2, observers adapted to a moving stereoscopic grating of a given cyclopean orientation and tested for the motion aftereffect with a static grating of the same or different orientation. Robust motion aftereffects were induced only when the orientation of the adapt and test stimuli was the same. Together, these results suggest that the stereoscopic motion aftereffect is tuned for cyclopean spatial frequency and orientation which, in turn, suggest that the stereoscopic motion aftereffect is mediated by low-level oriented spatial-frequency mechanisms.

Adaptation, Physiological↗
PubMed2000

Asymmetries of saccadic eye movements in oriented-line-target search.

Visual search for a line-element target differing sufficiently in orientation from a background of line elements can be performed rapidly, effortlessly, and without eye movements. There is, however, a response asymmetry: detection is better with an oblique target element in vertical or horizontal background elements than when these orientation are interchanged. If the underlying visual mechanisms also provide an input to the oculomotor system, then a similar asymmetry should be observed in eye-movement behaviour. To test this hypothesis, an experiment was undertaken in which eye movements were recorded while subjects searched for a line-element target in background of line elements; orientations were chosen from the range 0 degree, 30 degrees, 60 degrees, and 90 degrees to the vertical. Data from three subjects showed that (1) latencies for the initial saccade, (2) angular errors in initial-saccade direction, and (3) manual response times depended similarly on the combination of target- and background-element orientations, performance being better for 30 degrees or 60 degrees targets in 0 degree or 90 degrees backgrounds than vice-versa. The early orientation-selective mechanisms responsible for the rapid detection of oriented-line targets are probably the same as those providing signals for saccadic eye movements.

Adult↗
PubMed2000

Perception of three-dimensional shape from texture is based on patterns of oriented energy.

This paper presents empirical support for a new observer model of inferring three-dimensional shape from monocular texture cues. By measuring observers' abilities to estimate the relative three-dimensional curvature along a textured surface from two-dimensional projected images, and concurrently examining the local spectral changes occurring in the projected image for various texture patterns, we have found that correlated changes in oriented energy along lines corresponding to the lines of maximum and minimum curvature of the surface are crucial for conveying the three-dimensional shape of the surface. Energy along these lines of maximum and minimum curvature can be used to compute the orientation of local surface patches. Texture patterns consisting of simple and complex sinusoidal gratings and plaids, and filtered noise were drawn onto a surface that was corrugated sinusoidally in depth about the horizontal axis and projected in perspective onto an image plane. The perceived relative surface curvature was reconstructed from measurements of local ordinal depth around a central fixation point at 12 different phases of the corrugation. Our results show that: (1) it is neither necessary nor sufficient to identify individual texture elements or texture gradients in order to extract the shape of the surface; (2) one-dimensional frequency modulation is insufficient for conveying complex three-dimensional shape. (3) Veridical ordinal depth is seen only when the projected pattern contains changes in oriented energy along lines corresponding to projected lines of maximum curvature of the surface. (4) For a surface corrugated in depth about the horizontal axis, this pattern of oriented energy arises from energy along the vertical direction in the global Fourier transform of the pre-corrugated pattern. (5) Local orientation changes across lines of minimum curvature can be also critical for conveying shape. (6) These correlated orientation changes along lines of maximum and minimum curvature are entirely lost in parallel projection. Hence texture is a useful cue for shape if the image is a perspective projection. (7) Only some natural textures will provide sufficient monocular cues to support veridical shape inferences, and this can be predicted from their global Fourier transforms.

Computer Simulation↗
PubMed2000

Association of fibroblast orientation around titanium in vitro with expression of a muscle actin.

The objective of this study was to investigate the association of cell orientation around a biomaterial with expression of a contractile actin isoform. Selected cytokines and a fungal metabolite known to alter the cytoskeleton were used to modulate the fibroblast orientation around titanium in vitro and the synthesis of a specific muscle actin in order to reveal an association between these processes. A novel culture system using a fibronectin-coated silicone surface was employed to evaluate the orientation of human gingival fibroblasts around titanium discs. Round glass cover slips, 25 mm in diameter, were coated with polydimethylsiloxane. During the heat-induced polymerization process, two commercially pure titanium discs, 5 mm in diameter, were placed on the silicone at a distance of approximately 0.5 mm apart. The rubbery consistency of the silicone stabilized the metal discs on the cover slip and eliminated the risk of developing a lip at the edge of the titanium sample. The cover slip was then heated to complete polymerization of the silicone and subsequently coated with fibronectin. One hundred thousand human gingival fibroblasts were plated onto each glass cover slip containing the titanium discs. The cells were treated with one of the following prior to seeding on the cover slips: transforming growth factor-beta1 (TGF-beta1), platelet-derived growth factor-BB (PDGF-BB), interferon-gamma (IFN-gamma) for cytochalasin-D. Untreated cells served as controls. The orientation of the cells at the surface of the titanium discs was evaluated microscopically and the cell content of alpha-smooth muscle actin (SMA) was determined by Western blot analysis and immunohistochemistry. A notable finding was the high correlation between the percentage of cells oriented perpendicular to the titanium surface and SMA synthesis. TGF-beta1, IFN-gamma and cytochalasin-D increased synthesis of SMA while PDGF-BB decreased it. The findings support the proposition that SMA-enabled cell contraction may play a role in the orientation of cells to a biomaterial surface.

Actins↗
PubMed2003

Binding and orientation of fibronectin to silanated glass surfaces using immobilized bacterial adhesin-related peptides.

Previously, we have demonstrated the suitability of bacterial adhesin-related peptides, directly immobilized on polystyrene surfaces, to bind and orient fibronectin (FN). For these studies a method to bind the large protein FN in a desired orientation on a solid substratum was developed which utilizes a bacterial adhesin-related peptide (designated BRP-A), which is known to bind specifically to the NH3-terminus end of FN. Glass substrata was first coated with an amine-terminated silane, followed by streptavidin (SA), which was used as an intermediate tether to bind the biotinylated bacterial adhesin-related peptide. The BRP-A peptide, used for these studies was synthesized with a terminal biotin to assure irreversible coupling of the BRP-A to the streptavidin. The biotinylated BRP-A was next immobilized on the SA-silanated glass surfaces. 125I-FN was used to quantify the amount of FN binding to the (BRP-A):SA-silanated glass surface. Monoclonal antibodies, which react with specific epitopes at either the NH3- or -COOH-termini of FN, were used to quantify the binding and orientation of FN. The results of these studies indicated: (1) FN bound to the BRP-A:SA-silanated glass surface; and (2) the bound FN was oriented such that NH2-terminal region of FN was bound towards the glass surface and the COOH-terminus was oriented away from the glass surface. These studies demonstrate that small peptides can be used to specifically bind and orient large proteins such as FN on the surfaces.

Adhesins, Bacterial↗
PubMed1999

Reliability and validity of first metatarsophalangeal joint orientation measured with an electromagnetic tracking device.

OBJECTIVE: To establish the reliability and validity of measurements of sagittal plane orientation of the first metatarsophalangeal joint using the Flock of Birds electromagnetic tracking device. DESIGN: Different joint orientations were reproduced on cadaver specimens in a specially designed jig with skin and skeletal application of the sensors. BACKGROUND: The Flock of Birds provides a means for quantifying first metatarsophalangeal joint motion, however, the reliability and validity of such an application has not been determined. METHODS: Joint orientation was measured in five cadaver feet with skin and skeletal sensor application. A specially designed jig allowed simulation of clinical tests of range of motion. Sagittal plane orientation was determined from 3-D, anatomically-based, reference frames embedded in the first metatarsal and proximal hallux. RESULTS: Reliability of all measurements was high for skin and skeletal sensor application. There were no significant differences in joint orientation between either technique for any of the simulated motion tests. CONCLUSIONS: The Flock of Birds provides reliable and valid measures of first metatarsophalangeal joint orientation with the sensors applied to the skin over the first metatarsal and proximal hallux. RELEVANCE: An electromagnetic tracking device is suitable for measuring sagittal plane motion of the first metatarsophalangeal joint. This motion can easily be expressed using 3-D, anatomically-based reference frames.

Cadaver↗
PubMed2001

A comprehensive approach to electro-orientation, electrodeformation, dielectrophoresis, and electrorotation of ellipsoidal particles and biological cells.

Suspended cells may respond to AC polarization by orienting, deforming, moving or rotating. For modeling of ellipsoidal cells, a new dipole approach is proposed. Along each of the principal axis of the model, three finite elements of arbitrary but equal cross-sectional area for the interior, low conductive membrane shell and exterior are assumed. The length of the external medium elements is defined by influential radii which are related to the depolarizing factors. The model predicts the potential at the ellipsoid's surface leading to the induced dipole moment. The moment obtained is identical to the Laplace approach for homogeneous ellipsoids; in the single-shell case, it is slightly different. The reason is the constant shell thickness which overcomes the confocal thickness necessary for the Laplace solution. Expressions for electro-orientation, deformation, dielectrophoresis, and electrorotation are derived. In linearly and circularly polarized fields, different orientation spectra are predicted to occur. While in linearly polarized AC fields, particles are oriented along their axis of highest polarizability, in circularly polarized fields, the axis of lowest polarizability is oriented perpendicular to the plane of field rotation. Based on this finding, a new electro-orientation method is proposed. In dielectrophoresis and electrorotation, reorientations are predicted which lead to discontinuous spectra.

Electrochemistry↗
PubMedDate not supplied

Second-order features extraction in the cat visual cortex: selective and invariant sensitivity of neurons to the shape and orientation of crosses and corners.

About 1/3 of neurons (52/174) studied in the cat striate cortex (area 17 or V1) gave a larger (by 3.2 times on average) response to a flashed cross or corner centered in the receptive field (RF) than to a single bar of optimal orientation. Most such neurons (71.4%) were found to be highly selective both to shape (angle between the lines) and to orientation of these figures. In the studied neuronal selection we also found all possible types of invariance of sensitivity to orientation and/or shape of these figures. We found neurons with selectivity to form of the figure and invariance to its orientation and vice versa. Some cells were found invariant both to form and orientation of the cross or corner but highly sensitive to flashing of any such figure in the RF. The role of RF center and surrounding area in sensitivity to cross figures was also studied in 44 additional units. Separated and combined stimulation of these zones revealed in different units summation, antagonism and absence of interaction of these zones by the selectivity index (figure/bar response ratio). Possible mechanisms of the described effects are discussed as well as their functional implication for second-order feature extraction in the visual cortex: selective or invariant sensitivity of neurons to the shape and orientation of the line-crossings.

Animals↗
PubMed2003

Orientation of human glioblastoma cells embedded in type I collagen, caused by exposure to a 10 T static magnetic field.

We investigated the preferred orientation of human glioblastoma cells (A172) following exposure to static magnetic fields (SMF) at 10 Tesla in the presence or absence of collagen. A172 cells embedded in collagen gel were oriented perpendicular to the direction of the SMF. A172 cells cultured in the absence of collagen did not exhibit any specific orientation pattern after 7 days of exposure to the SMF. Thus we succeeded in evoking the magnetic orientation of human glioblastoma cells by exposure to the SMF. Our results suggest that the orientation of glioblastoma cell processes may be due to the arrangement of microtubules under the influence of magnetically oriented collagen fiber.

Cell Division↗