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PubMed1990

Discrepancy between in vitro and in vivo assays for the susceptibility of Bacillus C.I.P. 5832 to antimicrobial agents.

In vitro, avoparcin, flavomycin, virginiamycin bacitracin, trimethoprim + sulfadimethoxine, colistin, spiramycin, oxolinic acid, lincomycin + spectinomycin and a mixture of organic acids were able to inhibit Bacillus C.I.P. 5832. In vivo, none of these antibacterial agents, at the levels incorporated in feeds, could affect the viable bacillus count in the caecal content of chickens. In another in vitro assay, olaquindox, carbadox and tylosin were also effective in inhibiting Bacillus C.I.P. 5832. But, concomitantly administered with carbadox or tylosin, Bacillus C.I.P. 5832 still promoted growth and feed conversion of fattening pigs. These results, as well as data of the quoted literature, suggest that the current in vitro techniques should not be used to screen the susceptibility of Bacillus C.I.P. 5832 to antimicrobial agents. Only in vivo assays seem to be of value.

Animals↗
PubMed1988

Staphylococcal infections in hospital: the Greek experience.

Resistance rates of various species to many antimicrobials are very high in Greece. Mean resistance rates of Staphylococcus aureus isolated in 12 Athens hospitals in the second half of 1986 were 80% for penicillin, 32% for methicillin, 22% for gentamicin, 27% for erythromycin, 17% for lincomycin and 16% for co-trimoxazole. In a prospective study in the General Hospital of Athens from May to July 1987, 40 coagulase-positive staphylococcal strains were isolated from various materials from inpatients. Of these 78% were resistant to penicillin, 50% to methicillin at 37 degrees C, 62% to methicillin at 30 degrees C, 38% to erythromycin, 32% to tobramycin, 15% to clindamycin, 15% to fusidic acid, 9% to amikacin, 5% to netilmicin and 0% to vancomycin. Ten of these strains (seven methicillin-resistant) were responsible for severe infections and three of the affected patients died (two nosocomial pneumonias, one infected burn, all due to methicillin-resistant strains). Another 11 strains (eight methicillin-resistant) were responsible for mild infections. From 41 coagulase-negative staphylococci isolated from inpatients, seven (five methicillin-resistant) were held responsible for mild infections. Of the 20 patients with infections due to methicillin-resistant strains, nine had previously received a beta- lactam antibiotic. Staphylococci are not responsible, however, for a large proportion of infections in our hospital at the moment. High resistance rates in Greece are due to overuse of antibiotics, a phenomenon attributed to a consumer's society behaviour of doctors and patients.

Anti-Bacterial Agents↗
PubMed1996

Oral species of Fusobacterium from human and environmental samples.

PURPOSE: The aim of this study was the characterization and identification of oral Fusobacterium in patients with and without periodontal disease, and from spittoons and air-water syringes. The antimicrobial susceptibility of this bacterium was evaluated. METHOD: Subgingival samples were taken using sterilized absorbent paper points. Spittoon samples were collected using sterile swabs around the drain area with shut off, and air-water syringe samples by washing the tip with Ringer solution. Samples were transferred in tubes under CO2 flux. Diluted samples were inoculated on to Omata and Disraely agar and blood agar plates, which were incubated in anaerobiosis, at 37 degrees C, for 4 days. Bacterial species were identified biochemically. MIC was determined using an agar dilution method. RESULTS: Periodontal patients, healthy subjects, spittoons and air-water syringes were 80%, 67.6%, 37.8% and 3.3% positive to Fusobacterium, respectively. Clindamycin, imipenem, lincomycin, metronidazole and tetracycline were active against all human and environmental isolates. Eighteen isolates resistant to ampicillin or penicillin G produced beta-lactamases. The presence of human oral bacteria in items of dental equipment supports the hypothesis that such equipment may serve as a vehicle for the transmission of pathogenic organisms. CONCLUSION: Pieces of dental equipment may serve as a vehicle for the transmission of oral pathogenic organisms.

Adolescent↗
PubMed1996

Staphylococcal infections in the Sofia Burn Centre, Bulgaria.

This study analysed staphylococcal infections in the Sofia Burns Centre in order to estimate their frequency, features and role in burns. For an 8-year period (1987-94) the bacterial aetiology of wound infections and bacteraemia in burned patients was studied. The prevalence of staphylococci in both wound exudation (31.4 per cent) and in blood cultures (60.7 per cent) was established. During the last year of the study there was a significant increase in the incidence of methicillin-resistant Staphylococcus aureus (MRSA) from 19.4 per cent in 1993 to 28.0 per cent in 1994 (P < 0.001). This raised serious therapeutic problems. MRSA were found more frequently in the ICU than in the wards and in wounds and blood cultures compared with other species/strains. MRSA caused infections in 18.8 percent of patients. Over 70 per cent of the MRSA strains were resistant to gentamicin, erythromycin and tetracycline and about one-third to lincomycin, co-trimoxazole, chloramphenicol and ciprofloxacin. All the MRSA strains were sensitive to vancomycin and 71.1 per cent to rifampicin. These findings show the necessity of urgent measures for restriction of the further distribution of MRSA infections in our burns centre.

Adolescent↗
PubMed1986

Nucleotide sequence of gene linA encoding resistance to lincosamides in Staphylococcus haemolyticus.

We have determined the nucleotide sequence of gene linA in plasmid pIP855, which confers resistance to lincomycin by inactivating it in Staphylococcus haemolyticus. The gene was defined by start and stop codons and an open reading frame of 483 bp corresponding to a product with an Mr of 19020. The apparent size of the resistance-conferring protein was 21 kDa, when a minicell extract was electrophoresed in the sodium dodecyl sulfate-polyacrylamide gels. Full expression of linA was obtained both in Escherichia coli and Bacillus subtilis.

Amino Acid Sequence↗
PubMed1987

Cloning and nucleotide sequence of a carbomycin-resistance gene from Streptomyces thermotolerans.

Two plasmids (pOJ158 and pOJ159) containing DNA fragments from the carbomycin(Cb)-producing strain Streptomyces thermotolerans were identified in Streptomyces griseofuscus based on their ability to confer resistance to Cb. The Cb-resistance determinants on pOJ158 and pOJ159 were designated carA and carB, respectively. In S. griseofuscus, pOJ159 also confers resistance to spiramycin, rosaramicin, lincomycin, and vernamycin B, but not to tylosin; in Streptomyces lividans, pOJ159 additionally confers resistance to erythromycin and oleandomycin. The carB gene was localized on pOJ159 to a 1.25-kb region whose nucleotide sequence was determined. The sequence has a G + C content of 68% and contains the coding sequence for carB and portions of the 5' and 3' untranslated regions. A comparison of the amino acid sequence of the protein encoded by carB (as deduced from the nucleotide sequence) with the deduced amino acid sequence of the RNA methylase from Streptomyces erythraeus (encoded by ermE) revealed extensive homology, suggesting that carB also encodes an RNA methylase. The region 5' to the coding sequence does not contain a small ORF or regions of complementarity that are commonly associated with translationally regulated macrolide-lincosamide-streptogramin B resistance genes. The 3' untranslated region contains an inverted repeat sequence that potentially can form a stable RNA stem-loop structure with a calculated delta G of -70 kcal.

Base Composition↗
PubMed1992

Resistance to macrolides and lincosamides in Streptomyces lividans and to aminoglycosides in Micromonospora purpurea.

Ribosomal (r) resistance to gentamicin in clones containing DNA from the producing organism Micromonospora purpurea is determined by grmA, and not by kgmA as originally reported. The kgmA gene originated in Streptomyces tenebrarius and is identical to kgmB. Both grmA and kgm encode enzymes that methylate single specific sites within 16S rRNA, although the site of action of the grmA product has not yet been determined. In either case, the methylated nucleoside is 7-methyl G. Inducible resistance to lincomycin (Ln) and macrolides in Streptomyces lividans TK21 results from expression of two genes: lrm, encoding an rRNA methyltransferase and mgt, encoding a glycosyl transferase (MGT), that specifically inactivates macrolides. The lrm product monomethylates residue A2058 within 23S rRNA (Escherichia coli numbering scheme) and confers high-level resistance to Ln with much lower levels of resistance to macrolides. Substrates for MGT, which utilises UDP-glucose as cofactor, include macrolides with 12-, 14-, 15- or 16-atom cyclic polyketide lactones (as in methymycin, erythromycin, azithromycin or tylosin, respectively) although spiramycin and carbomycin are not apparently modified. The enzyme is specific for the 2'-OH group of saccharide moieties attached to C5 of the 16-atom lactone ring (corresponding to C5 or C3 in 14- or 12-atom lactones, respectively). The lrm and mgt genes have been cloned and sequenced. The deduced lrm product is a 26-kDa protein, similar to other rRNA methyltransferases, such as the carB, tlrA and ermE products, whereas the mgt product (deduced to be 42 kDa) resembles a glycosyl transferase from barley.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1991

Corynebacterium pseudotuberculosis: in vitro susceptibility to 39 antimicrobial agents.

The minimal inhibitory concentrations of 39 antimicrobial agents for 54 isolates of Corynebacterium pseudotuberculosis in vitro have been determined. The most active agents were penicillins, macrolides, tetracyclines, cephalosporins, lincomycin, chloramphenicol, and rifampicin. Most isolates were resistant to aminoglycosides, nitrofurans, polymyxins, nalidixic acid, and cycloheximide.

Animals↗
PubMed1991

In vitro susceptibility of rabbit strains of Clostridium spiroforme to antimicrobial agents.

Using an agar dilution method we measured the minimum inhibitory concentration (MIC) of 12 antimicrobial agents against 11 strains of iota-toxigenic strains of Clostridium spiroforme. Each strain was isolated from a separate outbreak of toxic diarrhoea of rabbits. Vancomycin and bacitracin, both agents used to treat intestinal clostridioses of humans and other animals, had a relatively high MIC (8 micrograms/ml or more). Metronidazole was uniformly active against C. spiroforme. With MIC of 8 micrograms/ml or more, both lincomycin (11 strains) and erythromycin (9 strains) were relatively inactive against C. spiroforme, conversely, penicillin G was active (MIC for 8 strains was 0.5 micrograms/ml or less). Exposure to any one of these drugs has been implicated as a predisposing factor for C. spiroforme mediated diarrhoea of rabbits. The greatest variation in MIC was seen for erythromycin (8-fold), penicillin G (8-fold) and tetracycline (16-fold).

Animals↗
PubMed1995

Molecular studies on avian strains of Pasteurella multocida in Australia.

A collection of 45 strains of Pasteurella multocida was assembled. The strains had been isolated from cases of fowl cholera in eastern Australia over 8 years, and included mainly type A strains. All the strains were examined for plasmids and resistance to 10 antimicrobial agents and most of the strains were examined for restriction fragment length polymorphism. Nine strains were assayed for pathogenicity for mice. Twenty strains yielded no plasmid. Seven contained a single plasmid of 1.3 kbp and 18 contained 2 plasmids, of 2.4 and 7.5 kbp. All the strains were resistant to streptomycin, trimethoprim and lincomycin while one strain was resistant to tetracycline. There was no correlation between plasmid content and resistance to antimicrobial agents. Three strains that lacked plasmids were highly virulent for mice, 6 strains containing plasmids were not. Restriction fragment length polymorphism generated by HpaII allowed the 39 strains that were tested to be divided into 10 groups.

Animals↗
PubMed1991

Studies on the antimicrobial activity of Nigella sativa seed (black cumin).

Filter paper discs impregnated with the diethyl ether extract of Nigella sativa seeds (25-400 micrograms extract/disc) caused concentration-dependent inhibition of Gram-positive bacteria represented by Staphylococcus aureus. Gram-negative bacteria represented by Pseudomonas aeruginosa and Escherichia coli (but not Salmonella typhimurium) and a pathogenic yeast Candida albicans. The extract showed antibacterial synergism with streptomycin and gentamicin and showed additive antibacterial action with spectinomycin, erythromycin, tobramycin, doxycycline, chloramphenicol, nalidixic acid, ampicillin, lincomycin and sulphamethoxyzole-trimethoprim combination. The extract successfully eradicated a non-fatal subcutaneous staphylococcal infection in mice when injected at the site of infection.

Animals↗
PubMed1988

In vitro susceptibility to 10 antibiotics of methicillin-resistant (MRSA) Staphylococcus aureus in Chile.

The in vitro activity of 10 antibiotics was determined for 231 strains of methicillin resistant (MRSA) Staphylococcus aureus. Oxacillin was a very good antibiotic to determine methicillin-resistance. Its agreement with methicillin-resistance was in all the strains tested. On the contrary, the correlation with nafcillin was established only in 95% of the strains tested. Cloxacillin and flucloxacillin are not good methicillin-resistance indicators. The strains tested against macrolides, such as erythromycin, and lincosamides, such as lincomycin and clindamycin presented a susceptibility of 68, 78, and 80%, respectively. All tested strains were susceptible to vancomycin.

Anti-Bacterial Agents↗
PubMed1994

Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex-polymerase chain reaction assay.

A polymerase chain reaction (PCR) test was developed in which the mecA gene responsible for the intrinsic resistance to oxacillin in Staphylococcus aureus and the gyrA gene, always present in this species, were amplified in one operation. Among the 468 clinical isolates tested, the results obtained for 454 of the isolates (97%) were consistent with those of MIC determination. Discrepant results were noted for strains with low-level oxacillin resistance (MICs, 4-8 micrograms/ml) and mecA gene negative. For these strains, susceptibility to oxacillin was restored in the presence of a beta-lactamase inhibitor, which suggested a resistance by penicillinase hyperproduction. In contrast, all of the high-level resistant strains (MICs, > 8 micrograms/ml) carried the mecA gene. The presence of this gene has frequently been associated with resistance to gentamicin, tetracycline, erythromycin, lincomycin, and pefloxacin. The PCR assay described in this study can be accomplished with ease and total confidence in the clinical microbiologic laboratory for a rapid and effective establishment of antistaphylococcal chemotherapy.

Bacterial Proteins↗
PubMed1988

Diarrhoea and constipation.

Drug-induced constipation is mostly caused by changes in gut motility, whilst diarrhoea is more frequently caused by an increase in intestinal fluid secretion. In both instances the drug has to reach the enteric nervous system or the enterocyte, either via the blood or from the lumen, in sufficient concentrations to affect the mediators that regulate motility and fluid transport. Diarrhoea and constipation are frequently mentioned as side-effects of drugs, and therapeutic agents for almost all organ systems have been implicated. However, both these side-effects are usually mild or moderate, and rarely necessitate interruption of drug treatment. An exception to this rule is the antibiotic-associated colitis seen in patients treated with antibiotics such as lincomycin or clindamycin; in principle almost all antibiotics may cause this severe and potentially life-threatening complication. Other rare forms of severe, drug-induced colitis and diarrhoea result from toxic or anaphylactic reactions against gold preparations, cytostatic agents and sulphonamides. Ischaemic colitis due to vascular complications has been described in some women taking oral contraceptives, and in patients treated with vasopressin or digitalis.

Constipation↗
PubMed2005

Evidence for the role of the oxygen-evolving manganese complex in photoinhibition of Photosystem II.

Photoinhibition of PSII occurs at the same quantum efficiency from very low to very high light, which raises a question about how important is the rate of photosynthetic electron transfer in photoinhibition. We modulated electron transfer rate and light intensity independently of each other in lincomycin-treated pea leaves and in isolated thylakoids, in order to elucidate the specific effects of light and PSII electron transport on photoinhibition. Major changes in the rate of electron transport caused only small changes in the rate of photoinhibition, suggesting the existence of a significant photoinhibitory pathway that contains an electron-transfer-independent phase. We compared the action spectrum of photoinhibition with absorption spectra of PSII components that could function as photoreceptors of the electron-transfer-independent phase of photoinhibition and found that the absorption spectra of Mn(III) and Mn(IV) compounds resemble the action spectrum of photoinhibition, showing a steep decrease from UV-C to blue light and a low visible-light tail. Our results show that the release of a Mn ion to the thylakoid lumen is the earliest detectable step of both UV- and visible-light-induced photoinhibition. After Mn release from the oxygen-evolving complex, oxidative damage to the PSII reaction center occurs because the Mn-depleted oxygen-evolving complex cannot reduce P680+ normally.

Cucurbita↗
PubMed2006

An exposure assessment for selected pharmaceuticals within a watershed in Southern Ontario.

Recent studies from a number of countries have shown that measurable concentrations of both human and veterinary pharmaceuticals can be found in a variety of environmental matrices such as surface and ground water, soils, and sediments. Few data are available that characterize the sources, exposure and effects of pharmaceuticals in the environment and there is clearly a need to define these parameters within a Canadian context. We present in this paper the first report in southern Ontario, Canada on the geographic and temporal distribution of pharmaceuticals detected within seven tributaries receiving primarily agricultural inputs in a typical watershed. Of the 28 pharmaceuticals surveyed, 14 were detected in the streams sampled (n=125). Temporal trends in concentration for five frequently detected pharmaceuticals show pulses occurring between May and November of 2003 at similar but varying times over the seasons, depending on the pharmaceuticals, flow rate, and precipitation. Fluctuations in concentration of ions indicative of agricultural run off, such as nitrate and phosphate, were not found to be useful predictors of changes in pharmaceutical concentration (P>0.4), however a significant correlation between dissolved organic carbon and monensin and carbamazepine concentrations were observed (P<0.013). Exposure profiles illustrating concentration distributions for three of the more prevalent pharmaceuticals detected, including lincomycin, monensin and carbamazepine, showed a log normal distribution, useful for calculating centiles of environmental concentrations. While distributions of estimated total potency of pharmaceuticals detected in the surface waters suggested small risks of environmental effects of mixtures to daphnia, green algae, Lemna gibba, and fish, the significance of non-target effects and impacts due to chronic low level exposures to chemical mixtures remains unclear.

Animals↗
PubMed2005

Occurrence and characterization of Aeromonas spp. in mussels from the Adriatic Sea.

Samples of mussels (Mytilus galloprovincialis) were collected from natural beds on the Adriatic Sea in front of Ancona Province (Marches, Central Italy) officially recognized for human consumption. The mussels were analyzed to evaluate the prevalence of Aeromonas spp. pathogenic strains. Out of 144 samples, 32 Aeromonas strains were isolated and 12 showed virulence and enterophatogenicity on mice. Isolates were also examined to check their sensitivity to different antimicrobial agents. All isolates were sensitive to imepenem, neomycin, ciprofloxacin, tetracycline, oxytetracycline, doxycicline, chloramphenicol, polymyxin B, and colistin sulfate, but resistant to penicillin, carbenicillin, cephalotin, sulfamethoxazole, lincomycin, novobiocin, and rifampicin. Multiple resistance to antimicrobial agents with different mechanism of action was observed. Owing to the high occurrence of pathogenic Aeromonas strains, the risk of developing human infections by eating raw or undercooked mussels is envisaged.

Aeromonas↗
PubMed2005

Antimicrobial resistance profiles of dairy and clinical isolates and type strains of enterococci.

The susceptibility to 30 antimicrobial agents was determined by the disk diffusion method for a collection of 172 enterococcal strains, including 96 isolates from dairy sources, 50 isolates of human and veterinary origin, and 26 reference strains from 24 different enterococcal species. Results were analysed by hierarchic numerical methods to cluster strains and to group antimicrobials according to similarity profiles. Resistance to 17 of the 30 antimicrobials showed to be correlated, leading to four groups reflecting the mode of action: quinolones (ofloxacin, enrofloxacin, ciprofloxacin and norfloxacin); macrolides (erythromycin, spiramycin), phenicols (cloramphenicol) and tetracyclins (tetracycline, oxytetracyclin); aminoglycosides (gentamicin, kanamycin) and lincosamides (clindamycin); penicillins (amoxicillin, ampicillin, penicillin G, piperacillin) and carbapenems (imipenem). Overall, the genus Enterococcus behaved as resistant to lincomycin, colistin, polimixin B and, with a few exceptions in dairy isolates, to methicillin. In general, all isolates were susceptible to vancomycin, cloramphenicol and fusidic acid. Clusters containing only dairy isolates were susceptible to the majority of antimicrobials tested, as opposed to clusters constituted only by clinical enterococcal isolates. Among the clinical isolates, 62% were highly multiresistant. Low level gentamicin resistance was found to be associated with clinical enterococci. Among dairy isolates, those that clustered with clinical isolates were both resistant to gentamicin and identified as Enterococcus faecalis. Resistance to macrolides, quinolones, penicillins and imipenem was found to be associated also with clinical environments, mainly with multiresistant isolates, contrary to what is generally agreed as a characteristic of the genus. Veterinary clinical isolates were mainly grouped with the multiresistant clinical human isolates. The 26 reference enterococcal strains were distributed in clusters with different antibiotic resistance profiles and were mainly clustered with dairy isolates.

Anti-Bacterial Agents↗