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PubMed1993

Accumulation of actin in subsets of pioneer growth cone filopodia in response to neural and epithelial guidance cues in situ.

Directed outgrowth of neural processes must involve transmission of signals from the tips of filopodia to the central region of the growth cone. Here, we report on the distribution and dynamics of one possible element in this process, actin, in live growth cones which are reorienting in response to in situ guidance cues. In grasshopper embryonic limbs, pioneer growth cones respond to at least three types of guidance cues: a limb axis cue, intermediate target cells, and a circumferential band of epithelial cells. With time-lapse imaging of intracellularly injected rhodamine-phalloidin and rhodamine-actin, we monitored the distribution of actin during growth cone responses to these cues. In distal limb regions, accumulation of actin in filopodia and growth cone branches accompanies continued growth, while reduction of actin accompanies withdrawal. Where growth cones are reorienting to intermediate target cells, or along the circumferential epithelial band, actin selectively accumulates in the proximal regions of those filopodia that have contacted target cells or are extending along the band. Actin accumulations can be retrogradely transported along filopodia, and can extend into the central region of the growth cone. These results suggest that regulation and translocation of actin may be a significant element in growth cone steering.

Actins↗
PubMed1998

Unique responses of differentiating neuronal growth cones to inhibitory cues presented by oligodendrocytes.

During central nervous system development, neurons differentiate distinct axonal and dendritic processes whose outgrowth is influenced by environmental cues. Given the known intrinsic differences between axons and dendrites and that little is known about the response of dendrites to inhibitory cues, we tested the hypothesis that outgrowth of differentiating axons and dendrites of hippocampal neurons is differentially influenced by inhibitory environmental cues. A sensitive growth cone behavior assay was used to assess responses of differentiating axonal and dendritic growth cones to oligodendrocytes and oligodendrocyte- derived, myelin-associated glycoprotein (MAG). We report that >90% of axonal growth cones collapsed after contact with oligodendrocytes. None of the encounters between differentiating, MAP-2 positive dendritic growth cones and oligodendrocytes resulted in growth cone collapse. The insensitivity of differentiating dendritic growth cones appears to be acquired since they develop from minor processes whose growth cones are inhibited (nearly 70% collapse) by contact with oligodendrocytes. Recombinant MAG(rMAG)-coated beads caused collapse of 72% of axonal growth cones but only 29% of differentiating dendritic growth cones. Unlike their response to contact with oligodendrocytes, few growth cones of minor processes were inhibited by rMAG-coated beads (20% collapsed). These results reveal the capability of differentiating growth cones of the same neuron to partition the complex molecular terrain they navigate by generating unique responses to particular inhibitory environmental cues.

Animals↗
PubMed2003

A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain.

Monoubiquitylation is a regulatory signal, like phosphorylation, that can alter the activity, location or structure of a protein. Monoubiquitin signals are likely to be recognized by ubiquitin-binding proteins that transmit the regulatory information conferred by monoubiquitylation. To identify monoubiquitin-binding proteins, we used a mutant ubiquitin that lacks the primary site of polyubiquitin chain formation as bait in a two-hybrid screen. The C-terminus of Vps9, a protein required in the yeast endocytic pathway, interacted specifically with monoubiquitin. The region required for monoubiquitin binding mapped to the Vps9 CUE domain, a sequence previously identified by database searches as similar to parts of the yeast Cue1 and mammalian Tollip proteins. We demonstrate that CUE domains bind directly to monoubiquitin and we have defined crucial interaction surfaces on both binding partners. The Vps9 CUE domain is required to promote monoubiquitylation of Vps9 by the Rsp5 hect domain ubiquitin ligase. Thus, we conclude that the CUE motif is an evolutionarily conserved monoubiquitin-binding domain that mediates intramolecular monoubiquitylation.

Amino Acid Motifs↗
PubMed1996

Chemical cues are necessary but insufficient for reproductive activation of female pine vole (Microtus pinetorum).

Among various arvicoline rodents, reproduction is influenced to varying degrees by social factors, including behavioral or chemical cues. Since previous research suggested that chemosignals from adult males reproductively activate female pine voles (Microtus pine-torum), we sought to determine specifically what types of stimuli promote the activation response. In these experiments, female were exposed to unfamiliar adult males, or to some combination of cues from males, or were housed alone. Using uterine mass as a measure of reproductive activation, we found that females were not activated by exposure either to male urine by itself or to male-soiled bedding by itself, but full contact with a male clearly resulted in heavier uteri. Females whose vomeronasal organs were surgically excised failed to undergo reproductive activation when housed with males. Finally, females allowed physical contact by being housed directly underneath males had heavier uteri than did females whose housing allowed contact only with the chemical cues from males. Among female arvicoline rodents, it appears that there exists a physiological continuum between absolute dependence on both contact and chemical cues from males vs. absolute independence for reproductive activation. The present results place female pine voles closer to the former extreme than to the latter.

Animals↗
PubMedDate not supplied

Using evidence to educate birthing center nursing staff about infant states, cues, and behaviors.

The authors sought to apply evidence from research to nursing practice. Research about infant states, cues, and behaviors was presented to a birthing center nursing staff and expectant parent class instructors. Posttest results indicated that the staff's knowledge and skill in interpreting infant behavior for parents increased after an educational session. The results are important, for research supports the idea that parent-infant attachment affects both parents and infants by promoting a loving relationship and improved infant development, a healthy self-image, and better relationships later in life. Cue sensitivity has been documented as the origin of parent-infant attachment. Cue sensitivity involves recognition of individualized infant body language and provision of an appropriate response. Parents who are sensitive to their infant's needs and who respond consistently and appropriately foster a mutually satisfying reciprocal interaction that leads to a healthy relationship. Incorporating information about infant states, cues, and behaviors into prenatal education can provide parents with an introduction to quality parent-child interactions.

Adult↗
PubMed1999

Imposed retinal image size changes--do they provide a cue to the sign of lens-induced defocus in chick?

BACKGROUND: Young chicks can adjust their eye growth to compensate for both imposed hyperopia and myopia (using negative and positive spectacle lenses); the rate of eye elongation increases in the former and slows in the latter case. This emmetropizing behavior implies that the eye can distinguish the sign and magnitude of defocus, although the identity of the cue(s) involved is unknown. As the spectacle lenses used in these studies generally introduce significant retinal image size differences that are in opposite directions for negative and positive lenses (minification vs. magnification), we asked whether retinal image size might provide the required sign information. METHODS: This question was addressed by manipulating retinal image size while keeping lens power constant. We also investigated the effect of eliminating other potential cues, accommodation and chromatic aberration, under these conditions. Three negative "size" lenses of approximately -11 D optical power were used, with 2 of the lenses producing magnification rather than minification as typical of negative lenses (i.e. +1.9% and +6.9% compared to -2.9%). The lenses were fitted monocularly to 7-day-old chicks, which were subsequently measured at 9 and 11 days of age (refractive error and axial dimensions). The same lens-wearing schedule was applied to two other groups of chicks that had monocular ciliary nerve section surgery to prevent accommodation 2 days posthatching; one of these groups was reared under monochromatic yellow light instead of white light. RESULTS: Near-perfect refractive compensation was seen by the end of the treatment period with all three lenses, for all three treatment groups, and there was also little difference in the rate of compensation among the various groups. In all cases, the typical responses of axial (mainly vitreous chamber) elongation and myopia were observed. CONCLUSIONS: That manipulations to retinal image size, which either decrease or reverse the usual effects of negative lenses, did not disrupt compensation to the imposed hyperopic defocus, even in the absence of accommodation and chromatic aberration cues, argues against imposed retinal image size changes being the directional cue to defocus in experimental emmetropization.

Accommodation, Ocular↗
PubMedDate not supplied

Differences in salivation to repeated food cues in obese and nonobese women.

In a series of studies we have shown that salivation, a cephalic phase preingestive response, habituates to repeated presentations of olfactory or gustatory cues in nonobese subjects. Previous research has studied the differences in anticipatory response to food cues in obese vs. nonobese subjects. This study was designed to assess if obese and nonobese females differed in their patterns of salivary response to repeated presentation of palatable food cues. The salivary response to 10 gustatory presentations of lemon yogurt was studied in 10 obese and 10 nonobese nonrestrained women. Results showed significant differences in the pattern of salivary responding, with obese subjects showing a significantly slower decline in salivation than nonobese subjects. These results are consistent with the hypothesis that obese women differ from nonobese women in their pattern of response to repeated food cues. The results are discussed in relationship to models of intake that focus on differences in satiety or differences in the reinforcing value of food between obese and nonobese subjects.

Adolescent↗
PubMed2001

Motivation for alcohol assessed by multiple variable interval schedule behaviour: effects of reward size and alcohol cues.

Altered motivation for drugs of abuse is a central feature of most definitions of drug dependence and the impact of drug-related cues on motivation is of current interest. However, since most studies of cue-reactivity have not used behavioural measures of motivation, their results are often difficult to interpret in motivational terms. In the current paper we describe two experiments in which a behavioural technique, based on multiple variable interval (VI) schedules of reinforcement, was used to study motivation for alcohol in human subjects. In both experiments, subjects attended for several sessions and, during each session, were exposed to a 6-ply VI schedule (values ranged from 1 to 720 s), during which they earned points that were later exchanged for a preferred beer or lager. In Experiment 1 the procedure was validated by showing that changes in the magnitude of reinforcement altered behaviour appropriately. In Experiment 2 we found evidence that the presence of an alcohol-related cue increased the value of alcohol rewards. The results are discussed with reference to a model for the behavioural effects of drug-related cues in triggering relapse and a number of problems we found in using the multiple variable interval schedule procedure.

Adult↗
PubMed1996

Image blur as a pictorial depth cue.

A range of cues are already known to mediate depth perception in pictures and have been exploited by artists in drawings and paintings. Modern images are commonly generated by photographic or video equipment, and these images contain a depth cue that cannot be found in artistic depictions of natural scenes: different image regions are often blurred by different amounts, because of depth of focus limitations. Demonstrations presented here show that this selective image blur also acts as a pictorial depth cue, even when other pictorial cues are removed. Experimental data indicate that the degree of blur at borders between blurred and sharp image regions is used by the visual system to establish the depth ordering of different regions. Selective image blur is thus a potentially useful addition to computer-generated and cartoon images to enhance the impression of depth they convey. It may well also contribute to depth perception in natural retinal images, because the depth of focus of the human eye is limited.

Computer Graphics↗
PubMed1999

Facial attractiveness, symmetry and cues of good genes.

Cues of phenotypic condition should be among those used by women in their choice of mates. One marker of better phenotypic condition is thought to be symmetrical bilateral body and facial features. However, it is not clear whether women use symmetry as the primary cue in assessing the phenotypic quality of potential mates or whether symmetry is correlated with other facial markers affecting physical attractiveness. Using photographs of men's faces, for which facial symmetry had been measured, we found a relationship between women's attractiveness ratings of these faces and symmetry, but the subjects could not rate facial symmetry accurately. Moreover, the relationship between facial attractiveness and symmetry was still observed, even when symmetry cues were removed by presenting only the left or right half of faces. These results suggest that attractive features other than symmetry can be used to assess phenotypic condition. We identified one such cue, facial masculinity (cheek-bone prominence and a relatively longer lower face), which was related to both symmetry and full- and half-face attractiveness.

Adolescent↗
PubMedDate not supplied

Using visual cues of contact to improve interactive manipulation of virtual objects in industrial assembly/maintenance simulations.

This paper describes a set of visual cues of contact designed to improve the interactive manipulation of virtual objects in industrial assembly/maintenance simulations. These visual cues display information of proximity, contact and effort between virtual objects when the user manipulates a part inside a digital mock-up. The set of visual cues encloses the apparition of glyphs (arrow, disk, or sphere) when the manipulated object is close or in contact with another part of the virtual environment. Light sources can also be added at the level of contact points. A filtering technique is proposed to decrease the number of glyphs displayed at the same time. Various effects--such as change in color, change in size, and deformation of shape- can be applied to the glyphs as a function of proximity with other objects or amplitude of the contact forces. A preliminary evaluation was conducted to gather the subjective preference of a group of participants during the simulation of an automotive assembly operation. The collected questionnaires showed that participants globally appreciated our visual cues of contact. The changes in color appeared to be preferred concerning the display of distances and proximity information. Size changes and deformation effects appeared to be preferred in terms of perception of contact forces between the parts. Last, light sources were selected to focus the attention of the user on the contact areas.

Journal Article↗
PubMed2006

Time course of attention for alcohol cues in abstinent alcoholic patients: the role of initial orienting.

OBJECTIVE: Addicted people are characterized by enhanced attention for drug cues leading to drug use. However, there is little research on the component processes of attention in individuals with alcoholism. Here, we examine 2 distinct components of attention in abstinent alcohol-dependent individuals and social drinkers of alcohol, that is to say, the initial orienting to alcohol-related cues, and the maintenance of attention to them. METHOD: The present study used an "alcohol" version of the visual probe detection task with alcohol-related or neutral pictures being presented briefly (i.e., 50 ms), to assess initial orienting, or longer (i.e., 500 and 1,250 ms), to assess the maintenance of attention. RESULTS: Only alcoholic patients were faster in detecting a probe displayed immediately after pictures related to alcohol presented for 50 ms than in detecting the same probe replacing non-alcohol-related pictures. However, when pictures were presented for 500 ms, only social alcohol drinkers were faster in detecting the probe replacing alcohol scenes. At a stimulus of 1,250 ms duration, no group showed attentional bias toward alcohol cues. In addition, the severity of alcoholism measured by the total number of prior detoxification treatments was positively correlated with the attentional bias (or "attraction") for alcohol pictures presented for 50 ms. CONCLUSIONS: These results show that, subsequent to initial visual orienting to alcohol-related cues, abstinent patients' attention was disengaged from these stimuli, thus suggesting a visual approach-disengagement attentional pattern. The influence of these findings on relapse was discussed.

Adult↗
PubMed1996

A comparison of the ovulation method with the CUE ovulation predictor in determining the fertile period.

The purpose of this study was to compare the CUE Ovulation Predictor with the ovulation method in determining the fertile period. Eleven regularly ovulating women measured their salivary and vaginal electrical resistance (ER) with the CUE, observed their cervical-vaginal mucus, and measured their urine for a luteinizing hormone (LH) surge on a daily basis. Data from 21 menstrual cycles showed no statistical difference (T = 0.33, p = 0.63) between the CUE fertile period, which ranged from 5 to 10 days (mean = 6.7 days, SD = 1.6), and the fertile period of the ovulation method, which ranged from 4 to 9 days (mean = 6.5 days, SD = 2.0). The CUE has potential as an adjunctive device in the learning and use of natural family planning methods.

Adult↗
PubMed1982

Short-term release cues to stop-consonant place of articulation in child speech.

In recent work Blumstein and Stevens (J. Acoust. Soc. Am. 66, 1001-1017 (19179); 67, 648-662 (1980) have proposed that the short-term onset spectra at stop-consonant releases provide invariant cues for the perception of place of articulation, and that the salient acoustic characteristics of these cues can be characterized by a set of templates. The present study investigated the suitability of these templates for characterizing place of articulation in utterance-initial voiced stop consonants produced by two children 71-75 weeks old in spontaneous discourse. The overall correct categorization rate for the templates was 65%, with 70% correct categorizations for the bilabial template, 85% for the alveolar template, and 40% for the velar template. The results of a perceptual study in which listeners were asked to identify place of articulation for computer-edited 15-, 25-, 40-, and 135-ms samples of the initial parts of these utterances were compared with the results of the template matching study. Although listeners' identification of place of articulation was at well above chance level on even the 15-ms tokens, there was no correlation between the results of the template-matching study and the perceptual data. The perceptual data thus indicate that short-term onset cues exist in the spontaneous speech of children at an early stage of language development, however, these cues do not appear to be adequately characterized by the Blumstein-Stevens templates.

Alveolar Process↗
PubMed1991

Discrimination of formant transition onset frequency: psychoacoustic cues at short, moderate, and long durations.

Two experiments determined the just noticeable difference (jnd) in onset frequency for speech formant transitions followed by a 1800-Hz steady state. Influences of transition duration (30, 45, 60, and 120 ms), transition-onset region (above or below 1800 Hz), and the rate of transition were examined. An overall improvement in discrimination with duration was observed suggesting better frequency resolution and, consequently, better use of pitch/timbre cues with longer transitions. In addition, falling transitions (with onsets above 1800 Hz) were better discriminated than rising, and changing onset to produce increments in transition rate-of-change in frequency yielded smaller jnd's than changing onset to produce decrements. The shortest transitions displayed additional rate-related effects. This last observation may be due to differences in the degree of dispersion of activity in the cochlea when high-rate transitions are effectively treated as non-time-varying, wideband events. The other results may reflect mechanisms that extract the temporal envelopes of signals: Envelope slope and magnitude differences are proposed to provide discriminative cues that supplement or supplant weaker spectrally based pitch/timbre cues for transitions in the short-to-moderate duration range. It is speculated that these cues may also support some speech perceptual decisions.

Attention↗
PubMed1992

Effects of roving level variation on monaural detection with a contralateral cue.

Monaural detection with a contralateral cue (MDCC) was measured with and without a 20-dB overall roving level to determine the contribution of loudness to performance on this task. Psychometric functions were obtained for three normal-hearing subjects as a function of the signal-to-noise ratio for pure-tone and 1/3-oct noiseband signals at 500 and 4000 Hz with a wideband noise masker. At 4000 Hz, the roving level degrades performance for the narrow-band noise signal by about 5.3 dB. In addition, the presence of the contralateral cue degrades performance for both the pure-tone and narrow-band noise signals at 4000 Hz by 3 to 6 dB. At 500 Hz, however, performance is not affected by the roving level, and is improved by 3 to 6 dB by the contralateral cue. These results indicate that loudness is being used as a cue only for detection of the 4000-Hz narrow-band noise.

Acoustics↗
PubMed1993

Function-based modeling of binaural processing: level and time cues.

From theoretical considerations, function-based modeling predicts the input-output characteristics of a neural system intended to perform a signal processing task within a sensory system. The sensory task under study here is the time- and level-based localization of a high-frequency, possibly amplitude-modulated, sound source in the horizontal plane. The stimulus is assumed to be represented by each ear's primary-like discharge pattern. An optimal system that extracts azimuthal angle from these discharge patterns, which represent acoustic time and level localization cues, has been derived. This system can be described as the maximization of a sum of three subsystems' outputs. The stimulus cues employed by these systems are interaural level difference for the level-based subsystem, the interaural onset-time difference for the time-based subsystem, and the interaural envelope-phase difference for the phase-based subsystem. The system encompassing all these cues is shown to trade-off the level, time, and envelope-phase cues depending upon the time since stimulus onset, the observation time, and the incident signal's level. How this system might correspond to known structures in the lower auditory pathway is described.

Acoustic Stimulation↗
PubMed1994

The development of the perception of cues to the [m]-[n] distinction in CV syllables.

The contribution of the nasal murmur and vocalic formant transition to the perception of the [m]-[n] distinction by adult listeners was investigated for speakers of different ages. Children, aged 3, 5, and 7, and an adult female and male produced consonant-vowel (CV) syllables consisting of either [m] or [n] and followed by [i ae u a]. Three productions of each syllable were computer edited into the following segments: (1) full murmur; (2) 50-ms murmur preceding release; (3) 25-ms murmur preceding release; (4) 25-ms murmur preceding release +25-ms transition following release; (5) 25-ms transition following release; (6) 50-ms transition following release; and (7) full transition+vowel. The results indicate that both the murmur and transition provide cues to place of articulation, but that the latter property is more prominent in perception than the former across speaker age. The salience of the murmur and vocalic transition cues was greater for adults than children indicating a developmental progression of the encoding of gestures associated with these properties. Although the simultaneous presence of murmur and vocalic transition cues surrounding the point of spectral discontinuity improved perception of place of articulation across speakers, there was evidence of a developmental progression of this property also. For speakers of all ages, as segment duration decreased, consistent decrements in identification of place of articulation occurred only for transition stimuli. The murmur+transition was the most salient cue supporting the importance of spectral discontinuities and/or relational properties in production and perception particularly in the acquisition of sound features.

Age Factors↗