Cesticidal action of diphenyl sulphone 4:4'-di-isothiocyanate.
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Immunomodulation of macrophage activity by in vitro secretions of Mesocestoides corti has been previously demonstrated. The modifying activity secreted by M. corti had the effect of reducing the normal accessory function of macrophages in a Con-A-activated lymphocyte proliferation assay. This paper describes the purification of the modifying activity by FPLC techniques and the generation of a monoclonal antibody (MoAb) to this molecule in mice. The MoAb bound immunomodulatory FPLC fractions of M. corti in an ELISA. When MoAb was applied in conjunction with immunomodulatory parasite secretions to macrophages in vivo or in vitro, the modifying effect of the secretions was abolished. This profound effect of the MoAb should help to elucidate the mechanisms by which metacestode parasites avoid host immune responses and may enable therapeutic intervention.
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Genus Coronacanthus Spassky, 1954 contains three species--C. integra (Hamann, 1891), C. omissa (Baer et Joyeux 1943) and C. vassilevi Genov, 1980--but larval stages of only first two species were described before. During 1991-1993 cysticercoids of all three species were found from gammarid crustaceans in Eastern Carpathians. The article contains an illustrated description of the cysticercoid of C. vassilevi. It differs considerably from the formerly known Coronacanthus larvae by the egg-shaped (not lens-shaped) form of the cyst, absence of marginal thickening of the cyst wall, by the shape and length of cercomer and besides, by the invagination of the anterior part of the scolex. Microvilli on the cyst surface were not detected with light microscope. Cercomer of C. vassilevi is narrow and very long as for mammalian hymenolepidid (4.7 to 8 times longer than cyst). The number (37-38), length (8-9 microns) and shape of rostellar hooks well correspond to those of adult worms (Genov, 1980).
The study by scanning electron microscope of the scolex of Cotugnia polyacantha, Raillietina (R.) echinobothrida, R (R.) tetragona, R. (R.) tunetensis and R. (Skrjabinia) cesticillus allowed us to demonstrate the presence, on the rostellum of each individual, of scale-like spines. We believe that these scale-like spines represent a new character of diagnosis for the whole of the Davaineidae.
Description of a new genus and new species of proteocephalid tapeworm in Paulicea luetkeni, which shows superficial resemblance to tetraphyllids, especially in its gravid proglottides. Euzetiella tetraphylliformis belongs to the Proteocephalidae, Proteocephalinae due to the presence of medullary gonads as well as the shape of the scolex and the presence of uniloculate suckers. Furthermore, this species is characterized by an apolytic strobila, which represents an exception within the Proteocephalidea.
Anthobothrium galeorhini n. sp. (Eucestoda: Phyllobothriidae) collected from the spiral intestine of Galeorhinus galeus (Linnaeus, 1758) at the Puerto Madryn (Atlantic coastal region) is described. The new species differs from A. cornucopia as described by Euzet (1959) by the size of body, bothridia, gravid proglottids and eggs; by the neck length and by the different number of testes and proglottids. The size of bothridia and the gravid proglottids, the cirrus length and the number of testes allow differentiating the species here proposed from A. laciniatum Linton, 1890 as described by Euzel (1959). The body and cirrus sac size, the bothridia shape and the number of testes separate the species here described from A. parvum Stossich, 1895 as described by Yamaguti (1952). The anatomic differences between the cestode described by Arandas Rego (1977) and located laxonomycally under the name Anthobothrium laciniatum are listed and the systematic position of the cestode is discussed. Species parasitic of skates listed in scientific literature must be taxonomically reaccommodated, since their characteristics are not consistent with the diagnosis of Anthobothrium.
Paranoplocephala etholeni n. sp., parasitizing the meadow vole Microtus pennsylvanicus in Alaska and Wisconsin, USA, is described. Paranoplocephala etholeni is morphologically most closely related to the Nearctic Paranoplocephala ondatrae (Rausch, 1948). Available data suggest that P. etholeni is a host-specific, locally rare species that may have a wide but sporadic geographical distribution in North America. The finding of P. ondatrae-like cestodes in Microtus spp. suggests that this poorly known species may actually be a parasite of voles rather than muskrat (type host). A tabular synopsis of all the known species of Paranoplocephala s. l. in the Holarctic region with their main morphological features is presented.
Scalithrium gen. n. (Cestoda, Tetraphyllidea) is proposed with Scalithrium minimum (Van Beneden, 1850) n. comb., parasite of Dasyatis pastinaca (Elasmobranchii, Dasyatidae) as type-species. The new genus Scalithrium (Tetraphyllidea, Phyllobothriidae, Rhinebothriinae) is erected for several species previously included in the genus Rhinebothrium. These species have a scolex with four bothridia, the distal surface of which is divided by transverse septa in a single row of loculi. Scalithrium minimum (Van Beneden, 1850) n. comb. is redescribed from specimens collected from the type-host Dasyatis pastinaca in Tunisia and becomes the type-species of the new genus. After Braun (1900) Echeneibothrium variabile Van Beneden, 1850 is considered as type-species of the genus Echeneibothrium. Species of Rhinebothriinae to be transferred into the genus Scalithrium are discussed and a key is proposed for the eight species.
Progamotaenia capricorniensis sp. nov. (Cestoda: Anoplocephalidae) is described from the wallabies Macropus dorsalis (Gray, 1837) and Petrogale assimilis Ramsay, 1877 from Queensland, Australia. The new species is characterised by a fimbriated velum composed of 26-32 digitiform to triangular projections on each side of the proglottis, paired uteri and 140-190 testes distributed in a single band across the medulla. Minor variation occurs in the distribution of the testes. The above characters distinguish the new species from its most closely related congeners P. lagorchestis (Lewis, 1914), P. proterogyna (Fuhrmann, 1932), P. spearei Beveridge, 1980 and P. villosa (Lewis, 1914). P. capricorniensis appears to exhibit a highly disjunct distribution within its usual host, M. dorsalis.
Four different types of trypanorhynch postlarvae were found attached to the stomach mucosa, external stomach wall or free in the body cavity of two marine fishes from Madeira, namely blackscabbard fish, Aphanopus carbo and oceanic horse mackerel, Trachurus picturatus. Morphological features shown by SEM indicated that the postlarvae belonged to the species Tentacularia coryphaenae, Sphyriocephalus tergestinus, Nybelinia lingualis and possibly N. yamaguitii. Prevalence [mean intensity (range)] of T. coryphaenae, S. tergestinus and Nybelinia spp. in A. carbo (n = 135) was 12.6% [1.65 +/- 1.27(1-6)], 5.9% [1.57 +/- 0.79 (1-3)] and 2.2% [1.33 +/- 0.58 (1-2)] respectively. The prevalence of T. coryphaenae and S. tergestinus showed some seasonality, with a rise in prevalence of T. coryphaenae corresponding to a decrease in prevalence of S. tergestinus. However these differences were not significant. In T. picturatus (n = 304) only N. lingualis was found at a prevalence of 9.6%. Both S. tergestinus and N. lingualis were recovered only from the stomach mucosa or external stomach wall, while T. coryphaenae was observed either attached to the stomach mucosa or free in the visceral cavity of the fish. The paper presents the first scanning electron micrographs (SEM) of Sphyriocephalus tergestinus and a new geographical record of N. lingualis in T. picturatus.
Elasmobranch specimens of lamnid and sphyrnid captured in 1999 in the State of Santa Catarina, Brazil, were parasitized with homeacanthoid trypanorhynch cestodes: Isurus oxyrinchus Rafinesque, 1810 with Nybelinia lingualis (Cuvier, 1817) Dollfus, 1929; Sphyrna zygaena (Linnaeus, 1758) with Heteronybelinia rougetcampanae (Dollfus, 1960) Palm, 1999. New details of internal morphology and/or scolex and/or proglottid surface ultrastructure are given. Adults of N. lingualis are reported for the first time in the Brazilian coast.