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Studies on the perception of taste: do primaries exist?

The idea that there are four primary tastes is basic to almost all research in gustation. Although this concept guides the formulation and interpretation of studies of taste psychophysics, as well as studies of taste stimuli, receptors, and neural organization, there is a surprising absence of studies designed to determine if this quadripartite view of taste is correct. The present group of three studies is designed to ask this question on a psychophysical level, comparing the taste data with combinations of tones, which appear to remain separate; subjects are asked: (a) whether tastes and tones--including mixtures--are perceived as singular or more-than-one, (b) whether increasing the number of components in a mixture (tastes or tones) results in increases in the perceived complexity, and (c) whether stimuli (tastes or tones) remain identifiable in mixtures with other stimuli. In all three experiments, it appears that the individual tones remained distinct and unaltered in combinations. Taste stimuli combined very differently from tones, in ways that suggested that the original components lost their identity, and sensations other than those in the original components (e.g., "primary tastes") were synthesized. From this it follows that taste is composed of many sensations, not just the "primary four."

Adolescent↗

Sweet taste and intake of sweet foods in normal pregnancy and pregnancy complicated by gestational diabetes mellitus.

BACKGROUND: Dietary compliance in gestational diabetes mellitus (GDM) is poor. Changes in sweet taste perception might alter food preferences in GDM, making dietary compliance difficult to achieve. These indexes have never been studied in GDM. OBJECTIVES: This study documented changes in sweet taste perception and dietary intakes in pregnant women with and without GDM and determined whether these differences persisted postpartum. DESIGN: Subjects were 30 pregnant women without GDM, 25 pregnant women with recently diagnosed GDM, and 12 nonpregnant control subjects. Pregnant women were tested at 28-32 wk gestation and retested 12 wk postpartum. Subjects evaluated the taste of strawberry-flavored milks with different sucrose (0-10%) and fat (0-10%) contents and glucose solutions (10-160 mmol/L). RESULTS: Women with GDM showed no differences in liking for the milk samples across test sessions and their liking ratings were not significantly different from those of nonpregnant control subjects. Women without GDM liked the 10% sucrose-sweetened milk samples less during pregnancy than at 12 wk postpartum (P </= 0.01), at which time their ratings were not significantly different from those of nonpregnant control subjects. In women with GDM, plasma glucose after a 50-g glucose load was correlated with both increased liking for the taste of glucose (r = 0.64, P </= 0.001) and higher consumption of fruit and fruit juices (r = 0.45, P </= 0.02). CONCLUSIONS: Normal pregnancy was associated with a lower preference for 10% sucrose-sweetened milk samples late in gestation than postpartum, whereas GDM was associated with no such differences. Plasma glucose in women with GDM was related to a higher preference for the sweet taste of glucose and higher dietary sweet-food intakes from fruit and fruit juices. These findings have important implications for the dietary management of GDM.

Adolescent↗

Taste intensity perception in aging.

Men (n = 91) and women (n = 79) between the ages of 23 and 88 years made cross-modal matches of distance to the perceived intensity of aqueous solutions representing the four basic taste qualities. Traditional analysis, which characterizes age-group performance in terms of the slope of a group's psychophysical function, yielded results consistent with previous findings in that some flattening of slope was observed in the oldest group. Analyses based on the slope of the functions generated by individual participants demonstrated significant regression on age for some qualities. None of these findings were observed in the analogous analysis of variance (ANOVA). Significant age-related changes in Intraclass Correlation Coefficient (ICC) were demonstrated by both ANOVA and regression analysis. The ICC reflects the consistency of an individual's discriminative response over repeated stimulus presentations. Its variation with age was dependent upon taste quality.

Adult↗

Sweet taste and diet in type II diabetes.

The relationship between sweet taste function and dietary intake was studied in 21 patients with type II diabetes mellitus and 16 age-, weight-, and sex-matched controls. Subjects rated the sweetness intensity and pleasantness of a series of beverage samples sweetened with sucrose: 1.5-24%, fructose: 1-18%, or aspartame: 0.25-4%. They also kept 7-day food records. No group differences were found in sweet taste perception, pleasantness ratings, daily energy intakes, or macronutrient composition of the diets. However, subjects with diabetes consumed less sucrose but 3.5 times more alternative sweeteners than did controls. Peak pleasantness ratings for the beverage samples were positively correlated with dietary sweetness content in the subjects with diabetes but not the controls. These findings suggest that in diabetes, hedonic ratings for a sweetened beverage were related to dietary sweetness intake rather than changes in sweet taste perception.

Adult↗

Adverse effects profile of sulfhydryl compounds in man.

Many of the adverse reactions produced by penicillamine and other compounds with an active sulfhydryl group form a distinctive pattern when viewed as a class. Alterations in taste perception, mucocutaneous lesions, proteinuria due to immune-complex membranous glomerulopathy, and pemphigus are adverse reactions that have been encountered with all of the compounds discussed herein. Hematologic reactions such as neutropenia and thrombocytopenia occur rarely and with variable frequency. The angiotension converting enzyme inhibitor captopril has an active sulfhydryl group. When it was first given in high doses to patients with severe hypertension, adverse effects similar in pattern to those just outlined were reported. With reduced doses and more careful patient selection, the more serious reactions are no longer found, but disturbances of taste perception, rash, and oral mucosal ulcers are still encountered.

Anemia, Aplastic↗

Testing taste sensitivity and aversion in very young children: development of a procedure.

Taste perception in 45 3- to 6-year-old children was tested using procedures specifically designed for this age group. Detection thresholds for sucrose and urea were measured by a staircase method and aversion to urea was assessed hedonically, using drawings of facial expressions. All children understood the task and could perform the necessary actions. A subgroup of 20 children participated in a second measurement after a mean interval of 9.5 days: there was a satisfactory degree of stability between the sessions. However, a third measurement, on a subgroup of 13 children after a somewhat longer interval, showed a marked drop in the stability of the urea thresholds. This drop was thought to arise from a decrease in the children's motivation, leading to increased distractibility. Mean threshold estimates were 31 mmol/l for sucrose detection, 59 mmol/l for urea detection and 134 mmol/l for urea aversion, but some children were extremely sensitive to the taste of urea. The findings show that it is possible to study taste perception in very young children if their age is taken into consideration in developing the test procedure. Valid data can be obtained if the procedures are short, easy to understand and intrinsically motivating.

Child↗

Ingestion patterns of food, water, saccharin and sucrose in streptozotocin-induced diabetic rats.

Changes in preference for sweet tasting solutions have previously been reported in diabetic rats. The present study was designed to use detailed analyses of drinking patterns to investigate changes in sweet taste perception in rats made diabetic with a streptozotocin (SZ) injection and tested with saccharin and a broad range of sucrose concentrations. Although meal patterns in diabetic rats have been studied extensively, drinking patterns and their relation to meal patterns have not been described. A second goal of this research was to use the detailed patterns of eating and drinking of the diabetic rat in an effort to understand the resulting hyperphagia. Rats were given IP injection of SZ or saline. Following the SZ injection, the rats showed marked increase in blood glucose levels, an increase in food and fluid intake and they failed to gain weight. The SZ-injected rats showed a loss of preference for saccharin and for high concentrations of sucrose. Patterns of sucrose ingestion that are correlated with taste perception were distorted in the diabetic rats. The detailed pattern analysis of eating showed that the diabetic-induced hyperphagia resulted from a marked increase in length of eating bouts. The increase in fluid intake in the diabetic rats resulted from both an increase in frequency and length of drinking bouts. Circadian patterns of both food and fluid ingestion were maintained in the diabetic rats.

Animals↗

Taste acuity of the human palate. II. Studies with electrogustometry on subjects in different age groups.

The electrical taste acuity on the hard and soft palate and the tip of the tongue was determined in 140 subjects aged 13-81 years using an electrogustometer constructed for the study. The possible influence of several variables including age, sex, wearing of a full upper denture, smoking as well as medical and social factors on the threshold values was also studied. Taste perception was demonstrated on the soft palate and on an area of the hard palate close to the junction of the hard and soft palate in most subjects. No perception of the electrical taste was found in eleven subjects (8%) on either the hard or the soft palate and in an additional 33 subjects (24%) on the hard palate alone and 2 subjects (1%) on the soft palate alone. The taste thresholds on the hard palate were very high compared to those on the soft palate and those on the tongue. Older subjects generally had higher thresholds than did younger subjects and the differences were greater for the thresholds on the hard and soft palate than for those on the tongue. No statistically significant differences in threshold values were found between women and men, denture wearers and non-denture wearers or smokers and non-smokers.

Adolescent↗

fMRI activation in response to odorants orally delivered in aqueous solutions.

During food intake flavor perception results from simultaneous stimulation of the gustatory, olfactory and trigeminal systems. Olfactory stimulation occurs mainly through the retronasal pathway and the resulting perception is often interpreted as a taste perception, thus leading to the well-known sensory confusion between taste and olfaction. The present experiment was designed to study, with functional magnetic resonance imaging (fMRI), the cortical representation of olfactory perception in humans in response to retronasal stimulation by odorants delivered in aqueous solution. Psychophysical evaluation confirmed that the stimuli acted as pure olfactory stimuli through the retronasal pathway and did not present any taste component. Results showed activation in all brain regions previously described with neuroimaging techniques using olfactory stimulation with an odorized air flow. Piriform and orbitofrontal cortex were found activated as well as the hippocampal region, the amygdala, the insular lobe, the cingulate gyrus and the cerebellum. These results demonstrate the feasibility of efficiently stimulating the olfactory system in an fMRI scanner through the retronasal pathway with liquids delivered to the oral cavity. The presentation of olfactory stimuli in liquids to the mouth is a realistic model for the study of food-related flavor perception. This stimulation protocol furthermore allows presenting taste and olfactory stimuli separately or combined, thus allowing for direct comparisons between single modality representation, taste or olfaction, and representation of multi-modality mixtures.

Administration, Oral↗

Effect of protease inhibitors on the sense of taste.

The purpose of this study was to investigate the taste properties of protease inhibitors which are essential components of drug regimes used to treat human immunodeficiency virus (HIV) infection. In this study, the taste properties of four protease inhibitors (indinavir, ritonavir, saquinavir, and nelfinavir) were investigated in unmedicated HIV-infected patients and healthy controls. Three of the four protease inhibitors (indinavir, ritonavir, and saquinavir) were found to be predominantly bitter (with additional qualities of medicinal, metallic, astringent, sour, and burning). Nelfinavir was found to be relatively tasteless. HIV-infected and uninfected control subjects detected protease inhibitors at similar concentrations, but HIV-infected subjects perceived suprathreshold concentrations as more bitter than controls. Detection thresholds ranged from 0.0061 mM for saquinavir in HIV-infected patients to 0.0702 mM for ritonavir in uninfected control subjects. Suprathreshold studies indicated that protease inhibitors modified the taste perception of a variety of other taste compounds. These results are consistent with clinical findings that protease inhibitors produce taste complaints that can impact patient compliance.

Adult↗

Neurochemical markers of human fungiform papillae and taste buds.

The presence of distribution of several neurochemical markers in human fungiform papillae and taste buds were investigated by the immunohistochemical technique. The gustatory cells of the taste buds are in synaptic contact with sensory nerve endings, and considering the taste buds strictly as specialized sensory organs, the amounts and distribution of some of the neurochemical markers were different to what we expected. For example, few structures showed immunoreactivity to the tachykinins substance P (SP), calcitonin gene-related peptide (CGRP), and neurokinin A (NKA) also for the peptides vasoactive intestinal polypeptide (VIP), neuropeptide tyrosine (NPY) and galanin, low amounts of immunoreactivity occurred. On the other hand, using antibodies to protein gene product 9.5 (PGP 9.5), protein S-100, and glutamate, numerous nerve fibres and/or immunoreactive cells were found in the fungiform papillae, in the epithelium, in the connective tissue and around blood vessels, as well as in or near taste buds. Incubation with the antibodies against somatostatin, enkephalin, bombesin, peptide histidine isoleucine amide (PHI), cholecystokinin (CCK)/gastrin and dopamine-beta-hydroxylase (DBH) was negative for the fungiform papillae. In conclusion, the present study has shown several immunoreactive structures using antibodies against certain neurochemical markers. Further investigations will hopefully correlate these morphological findings with functional taste perception data. Future studies of patients with taste disorders or other pathological changes correlated with taste and tongue will also be of utmost importance.

Antibodies↗

Smell and taste disorders, a study of 750 patients from the University of Pennsylvania Smell and Taste Center.

Smell and taste disorders are common in the general population, yet little is known about their nature or cause. This article describes a study of 750 patients with complaints of abnormal smell or taste perception from the University of Pennsylvania Smell and Taste Center, Philadelphia. Major findings suggest that: chemosensory dysfunction influences quality of life; complaints of taste loss usually reflect loss of smell function; upper respiratory infection, head trauma, and chronic nasal and paranasal sinus disease are the most common causes of the diminution of the sense of smell, with head trauma having the greatest loss; depression frequently accompanies chemosensory distortion; low body weight accompanies burning mouth syndrome; estrogens protect against loss of the sense of smell in postmenopausal women; zinc therapy may provide no benefit to patients with chemosensory dysfunction; and thyroid hormone function is associated with oral sensory distortion. The findings are discussed in relation to management of patients with chemosensory disturbances.

Burning Mouth Syndrome↗

The taste of monosodium glutamate (MSG), L-aspartic acid, and N-methyl-D-aspartate (NMDA) in rats: are NMDA receptors involved in MSG taste?

Monosodium glutamate (MSG) is believed to elicit a unique taste perception known as umami. We have used conditioned taste aversion assays in rats to compare taste responses elicited by the glutamate receptor agonists MSG, L-aspartic acid (L-Asp), and N-methyl-D-aspartate (NMDA), and to determine if these compounds share a common taste quality. This information could shed new light upon the receptor mechanisms of glutamate taste transduction. Taste aversions to either MSG, L-Asp or NMDA were produced by injecting rats with LiCl after they had ingested one of these stimuli. Subsequently, rats were tested to determine whether they would ingest any of the above compounds. The results clearly show that a conditioned aversion to MSG generalized to L-Asp in a dose-dependent manner. Conversely, rats conditioned to avoid L-Asp also avoided MSG. Conditioned aversions to MSG or L-Asp generalized to sucrose when amiloride was included in all solutions. Importantly, aversions to MSG or L-Asp did not generalize to NMDA, NaCl or KCl, and aversions to NMDA did not generalize to MSG, L-Asp, sucrose or KCl. These data indicate that rats perceive MSG and L-Asp as similar tastes, whereas NMDA, NaCl and KCl elicit other tastes. The results do not support a dominant role for the NMDA subtype of glutamate receptors in taste transduction for MSG (i.e. umami) in rats.

Animals↗

Candidate taste receptors in Drosophila.

Little is known about the molecular mechanisms of taste perception in animals, particularly the initial events of taste signaling. A large and diverse family of seven transmembrane domain proteins was identified from the Drosophila genome database with a computer algorithm that identifies proteins on the basis of structure. Eighteen of 19 genes examined were expressed in the Drosophila labellum, a gustatory organ of the proboscis. Expression was not detected in a variety of other tissues. The genes were not expressed in the labellum of a Drosophila mutant, pox-neuro70, in which taste neurons are eliminated. Tissue specificity of expression of these genes, along with their structural similarity, supports the possibility that the family encodes a large and divergent family of taste receptors.

Algorithms↗

Changes in patients' taste acuity after Roux-en-Y gastric bypass for clinically severe obesity.

OBJECTIVE: Patients who have undergone Roux-en-Y gastric bypass (RYGB) for clinically severe obesity often report an aversion to sweet foods and meat. This study was designed to determine whether changes in taste acuity occur after RYGB. DESIGN: This prospective, repeated measures trial evaluated acuity for sweet (sucrose) and bitter (urea) tastes, zinc status, and reported changes in taste perceptions in patients undergoing RYGB for weight reduction. SUBJECTS: Taste acuity and serum zinc level were measured in 14 subjects, 6 men and 8 women (mean preoperative body mass index [calculated as kg/m2] = 60.8 +/- 11.8 and mean age = 38.4 +/- 6 years), before surgery and 6 and 12 weeks after surgery. Inquiries regarding taste preferences were made at each postoperative visit with specific reference to sweets and meat. Serum zinc level was measured at the same visit. A noncontrolled comparison group of 4 subjects who were consuming a very-low-calorie diet also underwent taste acuity testing at similar intervals. MAIN OUTCOME MEASURES: The main outcome measures were recognition taste thresholds, serum zinc levels, and taste preference changes. STATISTICAL ANALYSIS: Analysis of variance with repeated measures over time, Pearson correlation coefficients, and post hoc analysis of variance were used to analyze data. RESULTS: Mean recognition thresholds for sucrose were 0.047 +/- 0.03 mol/L preoperatively and fell significantly to 0.024 +/- 0.01 and 0.019 +/- 0.01 mol/L at 6 and 12 weeks postoperatively, respectively. Overall, there were no significant differences in taste thresholds for urea over time; a significant difference was noted, however, in the pattern of change for urea between patients who reported an aversion to meats and those who did not. Zinc concentrations did not change during the study. APPLICATION/CONCLUSIONS: At 6 weeks postoperatively, all patients reported that foods tasted sweeter, and they modified food selection accordingly. Six patients reported an aversion to meats associated with increased nausea and vomiting. Acuity for sweet and bitter tastes may need to be considered when planning dietary modifications for patients undergoing RYGB.

Adult↗

Issues of gustatory neural coding: where they stand today.

The basic issues of gustatory neural coding are revisited. Questions addressed and conclusions drawn are: (1) what is the physical dimension across which gustatory neurons are sensitive, and upon which taste perceptions are based? The dimension that unites the various taste qualities is not physical, but physiological: a dimension of well-being, bounded by toxins at one extreme and nutrients at the other. (2) How broadly tuned are taste cells across the dimension? There are instances of specificity, but most mammalian taste cells respond to a range of qualities. (3) Are there basic taste qualities? Sweet, salty, sour, and bitter are widely accepted as basic tastes. Umami and starch tastes are considered basic by some. (4) Is taste topographically organized? There is some degree of physical separation among neurons most responsive to different taste qualities, but this does not appear to be sufficient precision to act as a meaningful coding mechanism. (5) Are there gustatory neuron types? Neurons, separated into categories according to their response profiles, respond as members of their category to the challenges of conditioned aversions and preferences, sodium deprivation, hyperglycemia, and receptor blockade, while cells from other categories react differently. This indicates the existence of functionally distinct types of taste cells. (6) Is the quality signal coded within the activity of the single most appropriate category of neurons, or is it carried by the pattern of response across neuronal categories? Both the breadth of responsiveness and the logical ambiguity of the signal in any one category of neurons argue that the taste message is carried by a pattern of activity across gustatory neuron types.

Animals↗