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Rachel weeping for her children.
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The 'weep and sleep' and 'fight or flight' systems.
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Weep for Adonais.
A 1995 motor-vehicle crash brought a tragic end to the promising medical career of Michael Agapitos, a specialist in physical medicine and rehabilitation who graduated from the University of Ottawa in 1982. The death of the popular young Ottawa physician and the subsequent court case involving the driver of the other vehicle have had a major impact on the victim's family and patients, Nicole Baer reports.
Weeping umbilicus: an unusual presentation of abdominal tuberculosis.
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When the weeping stopped: an otologist views otorrhea and gentamicin.
Gentamicin sulfate ophthalmic solution, 0.3%, was used ototopically in the treatment of 300 patients with otorrhea associated with acute and chronic ear conditions and to obtain a preoperative "dry state" in 93 cases in which surgical operation was indicated. Bacterial cultures, antibiograms, and audiometric tests were conducted before, during, and after ototopical therapy. Gentamicin-susceptible pathogens were isolated in all cases. A totally "dry state", after three weeks of therapy, was considered evidence of clinical success. Administration of gentamicin was by means of a wick or at the rate of 0.3 to 0.4 ml twice daily. Clinical success was observed in 271 cases. There were no signs of ototoxicity and only one reported instance of skin reaction. This simple, inexpensive treatment is particularly adaptable to situations in which specialized bacteriological testing facilities are unavailable and a broad-spectrum antibiotic is indicated.
Shoot circumnutation and winding movements require gravisensing cells.
Circumnutation and winding in plants are universal growth movements that allow plants to survive despite their sessile nature. However, the detailed molecular mechanisms controlling these phenomena remain unclear. We previously found that a gravitropic mutant of Japanese morning glory (Pharbitis nil or Ipomoea nil), Shidare-asagao (weeping), is defective not only in circumnutation but also in the winding response. This phenotype is similar to that of the Arabidopsis SCARECROW (SCR) mutant. We therefore investigated whether morning glory SCR (PnSCR) is involved in the weeping phenotype. We found that one amino acid was inserted into the highly conserved VHIID motif in weeping-type PnSCR; this mutation caused abnormal endodermal differentiation. We introduced either the mutant or WT PnSCR into Arabidopsis scr mutants for complementation tests. PnSCR of the WT, but not of weeping, rescued the shoot gravitropism and circumnutation of scr. These results show that both the abnormal gravitropism and the circumnutation defect in weeping are attributable to a loss of PnSCR function. Thus, our data show that gravisensing endodermal cells are indispensable for shoot circumnutation and the winding response and that PnSCR is responsible for the abnormal phenotypes of weeping.
The effect of temperature on the rate of cyanide metabolism of two woody plants.
The response of cyanide metabolism rates of two woody plants to changes in temperature is investigated. Detached leaves (1.0 g fresh weight) from weeping willow (Salix babylonica L.) and Chinese elder (Sambucus chinensis Lindl.) were kept in glass vessels with 100ml of aqueous solution spiked with potassium cyanide for a maximum of 28 h. Ten different temperatures were used ranging from 11 degrees C to 32 degrees C. The disappearance of aqueous cyanide was analyzed spectrophotometrically. The cyanide removal rate of Chinese elder was higher than that of weeping willow at all temperatures. The highest cyanide removal rate for Chinese elder was found at 30 degrees C with a value of 12.6 mg CN kg(-1) h(-1), whereas the highest value of the weeping willow was 9.72 mg CN kg(-1) h(-1) at 32 degrees C. The temperature coefficient values, Q10, which are the ratio of removal rates at a 10 degree difference, were determined for Chinese elder and weeping willow to 1.84 and 2.09, respectively, indicating that the cyanide removal rate of weeping willow was much more susceptible to changes in temperature than that of the Chinese elder. In conclusion, changes in temperature have a substantial influence on the removal rate of cyanide by plants.
Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses.
We have identified genes for pathogenicity toward rice (Oryza sativa) and genes for virulence toward specific rice cultivars in the plant pathogenic fungus Magnaporthe grisea. A genetic cross was conducted between the weeping lovegrass (Eragrostis curvula) pathogen 4091-5-8, a highly fertile, hermaphroditic laboratory strain, and the rice pathogen O-135, a poorly fertile, female-sterile field isolate that infects weeping lovegrass as well as rice. A six-generation backcrossing scheme was then undertaken with the rice pathogen as the recurrent parent. One goal of these crosses was to generate rice pathogenic progeny with the high fertility characteristic of strain 4091-5-8, which would permit rigorous genetic analysis of rice pathogens. Therefore, progeny strains to be used as parents for backcross generations were chosen only on the basis of fertility. The ratios of pathogenic to nonpathogenic (and virulent to avirulent) progeny through the backcross generations suggested that the starting parent strains differ in two types of genes that control the ability to infect rice. First, they differ by polygenic factors that determine the extent of lesion development achieved by those progeny that infect rice. These genes do not appear to play a role in infection of weeping lovegrass because both parents and all progeny infect weeping lovegrass. Second, the parents differ by simple Mendelian determinants, "avirulence genes," that govern virulence toward specific rice cultivars in all-or-none fashion. Several crosses confirm the segregation of three unlinked avirulence genes, Avr 1-CO39, Avr 1-M201 and Avr1-YAMO, alleles of which determine avirulence on rice cultivars CO39, M201, and Yashiro-mochi, respectively. Interestingly, avirulence alleles of Avr1-CO39, Avr1-M201 and Avr1-YAMO were inherited from the parent strain 4091-5-8, which is a nonpathogen of rice. Middle repetitive DNA sequences ("MGR sequences"), present in approximately 40-50 copies in the genome of the rice pathogen parent, and in very low copy number in the genome of the nonpathogen of rice, were used as physical markers to monitor restoration of the rice pathogen genetic background during introgression of fertility. The introgression of highest levels of fertility into the most successful rice pathogen progeny was incomplete by the sixth generation, perhaps a consequence of genetic linkage between genes for fertility and genes for rice pathogenicity. One chromosomal DNA segment with MGR sequence homology appeared to be linked to the gene Avr1-CO39. Finally, many of the crosses described in this paper exhibited a characteristic common to many crosses involving M. grisea rice pathogen field isolates.(ABSTRACT TRUNCATED AT 400 WORDS)
The PWL host specificity gene family in the blast fungus Magnaporthe grisea.
The PWL2 gene, isolated from a Magnaporthe grisea rice pathogen, prevents this fungus from infecting a second host grass, weeping lovegrass. We have investigated the distribution of sequences homologous to PWL2 in M. grisea strains isolated from diverse grass species. Multiple PWL2 homologs with varying degrees of sequence homology were identified. The presence of PWL2 homologs does not correlate with an avirulent phenotype on weeping lovegrass in many cases: some strains were fully pathogenic on weeping lovegrass although they carry multiple PWL2 homologs. Three weakly hybridizing PWL2 homologs were cloned and characterized. One of these, the PWL1 gene previously identified by genetic analysis, functioned to prevent infection of weeping lovegrass. Cloned PWL3 and PWL4 genes were nonfunctional, although PWL4 became functional if its expression was driven by either the PWL1 or the PWL2 promoter. The PWL1, PWL2, and PWL3/PWL4 genes map to different genomic locations. The amino acid sequences of the predicted PWL1, PWL3, and PWL4 proteins have 75, 51, and 57% identity, respectively, to the PWL2 protein. Our studies indicate that PWL genes are members of a dynamic, rapidly evolving gene family.
Effect of gravity on apical dominance in Pharbitis nil.
When the upper part of main shoot of morning glory (Pharbitis nil) is gently bent down, lateral bud on the bending region is released from apical dominance and starts to elongate. But, clinorotating the bending shoots prevents the release of the lateral bud from apical dominance. These results suggest that gravity affects apical dominance in morning glory. Here we verified the gravity-regulated apical dominance by using a weeping morning glory defective in gravitropic response due to abnormal differentiation of endodermis. That is, bending main shoot of the weeping morning glory hardly caused the lateral bud to elongate. In addition, decapitation of apical bud released the lateral bud from apical dominance, and exogenous auxin applied to the cut surface of the decapitated stem was inhibitory to the outgrowth of the lateral bud in the wild type. However, the effect of auxin was much less in the weeping morning glory. Thus, apical dominance of the weeping morning glory was weaker and less influenced by gravity than that of the wild type, which could occur due to abnormal differentiation of endodermis required for graviperception.
Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus.
Genetic analysis of host specificity in the rice blast fungus (Magnaporthe grisea) identified a single gene, PWL2 (for Pathogenicity toward Weeping Lovegrass), that exerts a major effect on the ability of this fungus to infect weeping lovegrass (Eragrostis curvula). The allele of the PWL2 gene conferring nonpathogenicity was genetically unstable, with the frequent appearance of spontaneous pathogenic mutants. PWL2 was cloned based on its map position. Large deletions detected in pathogenic mutants guided the gene cloning efforts. Transformants harboring the cloned PWL2 gene lost pathogenicity toward weeping lovegrass but remained fully pathogenic toward other host plants. Thus, the PWL2 host species specificity gene has properties analogous to classical avirulence genes, which function to prevent infection of certain cultivars of a particular host species. The PWL2 gene encodes a glycine-rich, hydrophilic protein (16 kD) with a putative secretion signal sequence. The pathogenic allele segregating in the mapping population, pwl2-2, differed from PWL2 by a single base pair substitution that resulted in a loss of function. The PWL2 locus is highly polymorphic among rice pathogens from diverse geographic locations.
Persistence of the effects of early experience on consumption of low-quality roughage by sheep.
The aim of this study was to determine the effects of experience early in life with cured weeping lovegrass (Eragrostis curvula) (low-quality roughage) or fresh oats (Avena sativa) (high-quality roughage) on voluntary intake and nitrogen retention when sheep ate a low-quality roughage 9 mo after initial exposure. From 1 to 5 mo of age, experienced wethers (EW) grazed cured weeping lovegrass, whereas inexperienced wethers (IW) grazed fresh oats (initial exposure). Then both EW and IW were fed sorghum (Sorghum bicolor) hay for 45 d, fresh weeping lovegrass for 105 d, and finally alfalfa hay for 120 d. Nine months after completion of the initial exposure EW and IW were offered sorghum hay (low-quality roughage), and, in addition, both groups were offered sorghum with ad libitum access simultaneously to alfalfa hay at six levels of availability. Experienced wethers ingested 15% more (P < .02) sorghum than IW. When given sorghum simultaneously with alfalfa at different levels of availability, EW ingested more (P < .10) sorghum than IW when only sorghum was freely available. Nitrogen retention was negative in both groups, but it was less negative in EW than in IW per unit of metabolic weight (P < .09) and per wether (P < .04). The results show that early dietary experience can have profound and persistent effects on consumption of foods low in nutritional quality, apparently through changes in critical physiological mechanisms.
[Ficus benjamina allergy].
We report the case of a 48-year-old patient suffered from asthma and conjunctivitis caused by an immediate type allergy to weeping fig (Ficus benjamina). By RAST inhibition test we could demonstrate that IgE antibodies react with allergens of fig; however our patient tolerated figs in oral provocation test. Sensitization to latex proteins reported to be cross reactive to Ficus species was not found. Ficus benjamina allergens represent relevant indoor allergens. A standardized allergen extract for skin testing is not yet available. Allergen specific IgE is mostly found in patients with strongly positive prick test results using the native sap of the tree. In 12 of 64 latex allergic patients we found simultaneous sensitization to weeping fig, so that cros-sensitization has to been considered in patients with IgE-mediated sensitization to latex.
The effects of reduced climbing and parallel fibre input on Purkinje cell dendritic growth.
The effects of afferent fibre depletion on the development of the dendritic trees of Purkinje cells in the cerbellum were investigated. Parallel fibres were reduced by postnatal administration of a schedule of low level X-irradiation. Climbing fibers were prevented from innervating the cerebellum by lesioning the olivo-cerebellar tract. Network analysis was performed on Purkinje cells in Golgi-Cox preparations of the vermis of 30-day-old animals. tin the irradiated cerebella Purkinje cells with a 'weeping willow' type morphology predominated. Purkinje cells devoid of their climbing fibre contact exhibited large spines on their main dendritic trunks. In both experimental situations the size of the dendritic tree was reduced. This diminution was the result of a decrease in the total number of dendritic segemnts. Individual segment lengths were largely unalteral. Topological type analysis revealed that the trees had arisen in a manner indistinguishable from terminal dichotomous branching and that the 'weep-willow' pattern was produced by a deviation of branching from a purely random form. The interaction of intrinsic and extrinsic factors in the formation of segments and on the nature of branching were discussed.
The effect of disc thickness and trauma on disc surface friction in the porcine temporomandibular joint.
The pathomechanics of osteoarthritis in the human temporomandibular joint (TMJ) are unknown. Compromised lubrication is a potential factor, but, lubrication within even the normal TMJ is not understood completely. Weeping lubrication is a concept that may be applicable to the TMJ. A characteristic of weeping lubrication is a slow increase in friction during static loading. The rate of increase in friction is related to the rate of lateral movement of synovial fluid away from the loading area. The TMJ disc is expected to be the main source of TMJ lubrication. This study tested two variables, disc thickness and magnitude of trauma to the disc, as factors that can affect the rate of flow of synovial fluid and thus alter lubrication of the disc surfaces. To test these variables, TMJ disc surface friction was measured before and after an impulse load. Before the impulse load, all discs demonstrated a gradual increase in friction during light static loading. The rate of increase in friction was inversely related to the disc thickness (R(2)=0.75). After an impulse load of known magnitude and peak force, disc surface friction was higher. The magnitude of this surface friction was correlated with the magnitude of the impulsive blow (R(2)=0.89) and the area of surface damage (R(2)=0.85). Disc thickness was a significant factor in determining the minimal impulse needed to produce higher surface friction (R(2)=0.99). These results confirm that disc thickness and trauma to the disc affect surface friction in the TMJ, and therefore may be important factors in compromised lubrication and the development of osteoarthritis.
The physiological and psychological effects of the expression and inhibition of emotion.
Previous work has indicated that humor enhances immunity, but the immunological effects of overt crying have not been studied. Thirty-nine women viewed sad and humorous videotapes and either inhibited or expressed overt expressions of laughter and weeping; these were observed and timed. Moods and immunity (S-IgA) were repeatedly measured. The humorous stimulus resulted in improved immunity, regardless of the overt laughter expressed; overt crying was immunosuppressive, whereas the inhibition of weeping in the context of the same sad stimulus was not. Moods were more negative following the sad stimulus and in the expression condition. The results with respect to humor are consistent with previous research; the immunosuppressive effect of overt crying is discussed in the context of various types of crying that may have different effects.