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Drinking water microbiology--new directions toward water quality enhancement.

Drinking water microbiology has emerged from decades of relative complacency to recognize there can be major concerns with potable water quality. Many of these issues are a result of an explosion of information on new waterborne agents, treatment problems with raw-source water qualities, biofilm development in some distribution systems and specialized requirements in water quality unique to hospitals and industries. Protozoan cyst survival after some disinfection practices involving surface water impoundments and virus occurrence in poorly protected groundwaters have provided reasons for expanding minimum treatment of surface waters and for requiring disinfection of all groundwaters unless there is a demonstrative data base to support exceptions in treatment requirements. Official monitoring of small water supplies must be increased on a monthly basis and a rapid alert established to inform water plant operators of unsatisfactory water qualities. As an option, application of operational tests to analyse water quality in terms of chlorine residual, turbidity, total coliforms and heterotrophic bacterial counts in small water plant operations should be encouraged. This would provide the operator at remote locations with the opportunity to utilize the information to make necessary treatment adjustments or corrections in water distribution deficiencies promptly and be a supplement to the official regional monitoring program. Application of drinking water alternative sources (bottled water and water from point-of-use treatment devices) should be viewed by the health authorities as only a temporary solution, not as a permanent fix for a public water supply known to present some established health risk to consumers. The public must also recognize that bottled water is not frequently monitored by health laboratories for acceptable quality and the use of home treatment devices places the responsibility of proper maintenance on the user. Microbial quality improvements in drinking water to hospitals and food industries can frequently be achieved through a routine, systematic flushing program for building plumbing networks and associated attachment devices. In other situations, use of booster disinfection or point-of-use devices may provide the important special water quality requirements for certain industrial applications. In any event, these supplemental treatment measures will require careful in-plant monitoring and maintenance to prevent reversals in water quality enhancement.

Animals↗

The efficacy of electrolysed oxidising water for inactivating spoilage microorganisms in process water and on minimally processed vegetables.

The efficacy of Electrolysed Oxidising Water (EOW) for inactivating spoilage microorganisms in process water and on minimally processed vegetables was investigated. The direct effect of EOW on three important spoilage bacteria namely; Pseudomonas fluorescens, Pantoea agglomerans or Rahnella aquatilis was determined by inoculating tap water or "artificial process water" with approximately 8 log CFU/ml pure culture and electrolysing the resultant solutions. The three bacteria were each reduced to undetectable levels at low (0.5 A) and relatively higher levels (1.0 A) of current in tap water and "artificial process water", respectively. The residual effect of EOW on P. fluorescens, P. agglomerans or R. aquatilis was determined by incubating at room temperature 1 ml (approximately 9 log CFU/ml) pure culture suspensions in 9 ml of EOW-T (EOW produced from tap water), EOW-A (EOW produced from "artificial process water" supplemented with approximately 60.7 mg Cl(-)/l and 39.3 mg Na(+)/l) or deionised water (control) for 0, 15, 45 or 90 min. The bactericidal activity of both EOW-T and EOW-A increased with the concentration of free oxidants and incubation period and the three bacteria were completely reduced at free oxidants-incubation period combinations of 3.88 mg/l-45 min and 5.1 mg/l-90 min in EOW-T and EOW-A, respectively. Two types of industrial vegetable process water; salad-mix and soup process water, which had each a total psychrotrophic count of approximately 8 log CFU/ml were then electrolysed. Without any NaCl addition, only 1.2 and 2.1 log reductions of the psychrotrophs in soup and salad-mix process water was attained respectively. Supplementation of the process water with approximately 60.7 mg Cl(-)/l and 39.3 mg Na(+)/l afterwards resulted in complete reduction of the psychrotrophic count in both process waters, but soup process water required relatively higher levels of current compared to salad-mix water. Finally, fresh-cut lettuce was washed in EOW-T containing 3.62 mg free oxidants/l, EOW-IP (EOW produced from industrial process water) containing 2.8 mg free oxidants/l or tap water (control) for 1 or 5 min. Washing the vegetables for 1 min in EOW-T resulted in 1.9, 1.2, and 1.3 log reductions of psychrotrophs, lactic acid bacteria and Enterobacteriacae, respectively, which increased to 3.3, 2.6, and 1.9 log reductions after washing for 5 min instead. EOW-IP tested in this work had no bactericidal effect on the microflora of fresh-cut lettuce. Electrolysis could therefore be used to decontaminate process water for vegetable pre-washing and to sanitise tap water for final rinsing of vegetables, respectively.

Colony Count, Microbial↗

A comparison of water quality indices for coastal water.

The present article discusses and compares five different water quality indices, viz arithmetic water quality index, multiplicative water quality index, unweighted arithmetic water quality index, unweighted multiplicative water quality index, and Harkin's water quality index, which were considered for characterizing the coastal water quality at the Jawaharlal Nehru Port Trust, Bombay, India. Dissolved oxygen, pH, biochemical oxygen demand (BOD), temperature, suspended solid, and turbidity were used as the parameters of water quality indices. The value function graphs used for above-mentioned variables were developed using harbour water quality standards and aquatic life. The product moment correlation coefficients for various water quality indices were determined using the SPSS software package to evaluate correlation among various indices. It was found that the unweighted arithmetic water quality index was higher than weighted arithmetic water quality index while the multiplicative water quality index was lower than unweighted multiplicative water quality index. All the indices were well correlated with each other except Harkin's water quality index. The Harkin's water quality index was different from other water quality indices. The comparison of different form of indices showed that the multiplicative water quality index was the most suitable water quality index for coastal waters.

Environmental Monitoring↗

Water uptake by roots: effects of water deficit.

The variable hydraulic conductivity of roots (Lp(r)) is explained in terms of a composite transport model. It is shown how the complex, composite anatomical structure of roots results in a composite transport of both water and solutes. In the model, the parallel apoplastic and cell-to-cell (symplastic and transcellular) pathways play an important role as well as the different tissues and structures arranged in series within the root cylinder (epidermis, exodermis, cortex, endodermis, stelar parenchyma). The roles of Casparian bands and suberin lamellae in the root's endo- and exodermis are discussed. Depending on the developmental state of these apoplastic barriers, the overall hydraulic resistance of roots is either more evenly distributed across the root cylinder (young unstressed roots) or is concentrated in certain layers (exo- and endodermis in older stressed roots). The reason for the variability of root Lp(r), is that hydraulic forces cause a dominating apoplastic flow of water around protoplasts, even in the endodermis and exodermis. In the absence of transpiration, water flow is osmotic in nature which causes a high resistance as water passes across many membranes on its passage across the root cylinder. The model allows for a high capability of roots to take up water in the presence of high rates of transpiration (high demands for water from the shoot). By contrast, the hydraulic conductance is low, when transpiration is switched off. Overall, this results in a non-linear relationship between water flow and forces (gradients of hydrostatic and osmotic pressure) which is otherwise hard to explain. The model allows for special root characteristics such as a high hydraulic conductivity (water permeability) in the presence of a low permeability of nutrient ions once taken up into the stele by active processes. Low root reflection coefficients are in line with the idea of some apoplastic bypasses for water within the root cylinder. According to the composite transport model, the switch from the hydraulic to the osmotic mode is purely physical. In the presence of heavily suberized roots, the apoplastic component of water flow may be too small. Under these conditions, a regulation of radial water flow by water channels dominates. Since water channels are under metabolic control, this component represents an 'active' element of regulation. Composite transport allows for an optimization of the water balance of the shoot in addition to the well-known phenomena involved in the regulation of water flow (gas exchange) across stomata. The model is employed to explain the responses of plants to water deficit and other stresses. During water deficit, the cohesion-tension mechanism of the ascent of sap in the xylem plays an important role. Results are summarized which prove the validity of the coehesion/tension theory. Effects of the stress hormone abscisic acid (ABA) are presented. They show that there is an apoplastic component of the flow of ABA in the root which contributes to the ABA signal in the xylem. On the other hand, (+)-cis-trans-ABA specifically affects both the cell level (water channel activity) and water flow driven by gradients in osmotic pressure at the root level which is in agreement with the composite transport model. Hydraulic water flow in the presence of gradients in hydrostatic pressure remains unchanged. The results agree with the composite transport model and resemble earlier findings of high salinity obtained for the cell (Lp) and root (Lp(r)) level. They are in line with known effects of nutrient deprivation on root Lp(r )and the diurnal rhythm of root Lp(r )recently found in roots of LOTUS.

Abscisic Acid↗

Evaluation of factors affecting survival of Escherichia coli in sea water. V. Studies with heat- and filter-sterilized sea water.

The bactericidal action of sea water was measured as the difference in survival of cells of Escherichia coli in untreated and autoclaved portions of water samples. The beneficial effect of sterilization by heat on the survival of E. coli in sea water varied with season and was most marked during summer months, however, the magnitude of the effect differed greatly from sample to sample. The more obvious and commonly suggested explanations for the bactericidal action of sea water were tested experimentally. The pH and salinity of sea water were changed by autoclaving, but the direction of the former was detrimental rather than beneficial and the significance of the latter was not clarified. The survival of cells of E. coli in filtered portions of some water samples was greater than that in untreated portions and equal to that in autoclaved portions, indicating that predators and competitors removed by filtration had contributed significantly to the rapid death of the bacterium in the untreated water. However, in the majority of samples tested, survival of E. coli in autoclaved water was considerably greater than survival in filtered water. The possibility that the beneficial effect of autoclaving over and above that of filtration resulted from inactivation or destruction by heat of bacteriophages and thermolabile toxic substances such as antibiotics was considered. Moreover, the suggestion was tested that the increased survival of E. coli in autoclaved sea water was due to the ability of heat to disrupt and degrade microbial cells and thermolabile compounds and, thereby, to cause an increase in concentration of available nutrients in sea water. It was concluded that the bactericidal action of sea water is not explicable in terms of the destruction or inactivation by heat of bacteriophages or antibiotics. Although added organic matter influenced the survival of E. coli, the test organism was not an effective competitor in sea water and the nutrient levels required to offset the bactericidal action were excessive. Artificial sea water was demonstrated to exert a bactericidal action comparable to that of natural sea water. Low levels of cysteine which favor survival of E. coli in natural sea water had a similar effect in artificial sea water. Nevertheless, it is not at this time possible to conclude that the factors responsible for the bactericidal action of artificial sea water are identical with those responsible in natural sea water.

Anti-Bacterial Agents↗

Effects of water source, dilution, storage, and bacterial and fecal loads on the efficacy of electrolyzed oxidizing water for the control of Escherichia coli O157:H7.

To evaluate the potential of using electrolyzed oxidizing (EO) water for controlling Escherichia coli O157:H7 in water for livestock, the effects of water source, electrolyte concentration, dilution, storage conditions, and bacterial or fecal load on the oxidative reduction potential (ORP) and bactericidal activity of EO water were investigated. Anode and combined (7:3 anode:cathode, vol/vol) EO waters reduced the pH and increased the ORP of deionized water, whereas cathode EO water increased pH and lowered ORP. Minimum concentrations (vol/vol) of anode and combined EO waters required to kill 10(4) CFU/ml planktonic suspensions of E. coli O157:H7 strain H4420 were 0.5 and 2.0%, respectively. Cathode EO water did not inhibit H4420 at concentrations up to 16% (vol/vol). Higher concentrations of anode or combined EO water were required to elevate the ORP of irrigation or chlorinated tap water compared with that of deionized water. Addition of feces to EO water products (0.5% anode or 2.0% combined, vol/vol) significantly reduced (P < 0.001) their ORP values to < 700 mV in all water types. A relationship between ORP and bactericidal activity of EO water was observed. The dilute EO waters retained the capacity to eliminate a 10(4) CFU/ml inoculation of E. coli O157:H7 H4420 for at least 70 h regardless of exposure to UV light or storage temperature (4 versus 24 degrees C). At 95 h and beyond, UV exposure reduced ORP, significantly more so (P < 0.05) in open than in closed containers. Bactericidal activity of EO products (anode or combined) was lost in samples in which ORP value had fallen to < or = 848 mV. When stored in the dark, the diluted EO waters retained an ORP of > 848 mV and bactericidal efficacy for at least 125 h; with refrigeration (4 degrees C), these conditions were retained for at least 180 h. Results suggest that EO water may be an effective means by which to control E. coli O157:H7 in livestock water with low organic matter content.

Colony Count, Microbial↗

Risk assessment of adenoviruses detected in treated drinking water and recreational water.

AIMS: Human adenoviruses (HAds) have previously been detected in sewage and polluted river and dam water, as well as treated drinking water. The 51 serotypes of HAds cause a wide range of infections with clinical manifestations associated with the gastrointestinal, respiratory and urinary tracts, and the eyes. Water may play a meaningful role in the transmission of many of these HAd serotypes, specifically the enteric HAds which are transmitted via the faecal-oral route. The presence of these viruses in water used for drinking and recreational purposes is considered to constitute a potential health risk. In this study, the risk of infection by the group of HAds previously detected over a period of 1 year in selected drinking water supplies, as well as river and dam water used for recreational purposes, was assessed. METHODS AND RESULTS: Adenoviruses were previously detected in nine of 204 (4.41%) samples of two drinking water supplies (A and B) treated and disinfected according to international specifications, in four of 51 (7.8%) samples of river water and nine of 51 (17.7%) samples of dam water. Application of these previously published results in an exponential risk assessment model indicated an annual risk of infection of 1.01 x 10(-1) and 1.7 x 10(-1) for drinking water supplies A and B, respectively, assuming a daily consumption of 2 l day(-1). The daily risk of infection constituted by HAds in the river water was calculated as 1.71 x 10(-4), and in the dam water as 3.12 x 10(-5), assuming a consumption of 30 ml of water per day. CONCLUSIONS: The risk of infection exceeded the tolerable risk of one infection per 10 000 consumers per year proposed for drinking water. However, the results for river and dam water used for recreational purposes were within the tolerable risk of one infection per 1000 bathers per day proposed for environmental waters used for recreational purposes. SIGNIFICANCE AND IMPACT OF THE STUDY: The study showed that the risk of HAd infection calculated for the drinking water supplies and the recreational water may overestimate the actual risk of infection, as conservative values were assumed for some of the variables. For a more accurate assessment of the potential risk of infection research should at least include a thorough investigation of the water consumption of individuals in South Africa, and the efficiency of recovery of the glass wool adsorption-elution method.

Adenoviridae↗

Effects of providing a water-soluble globulin in drinking water and diet complexity on growth performance of weanling pigs.

Two experiments were conducted to evaluate the effects of providing a water-soluble globulin in the drinking water on growth performance of weanling pigs. In Exp. 1, 360 weanling pigs (5.0 +/- 1.2 kg; 17 +/- 3 d of age; PIC) were blocked by initial weight and allotted to one of six treatments in a 2 x 3 factorial arrangement. Treatments included three diet complexity regimens with or without water-soluble globulin (3 and 1.5% solutions; d 0 to 7 and d 0 to 14, respectively) provided in the drinking water. The 35-d study was divided into three phases (d 0 to 7, 7 to 14, and 14 to 35) with corresponding lysine levels of 1.6, 1.5, and 1.35%. Soybean meal replaced specialty protein and lactose sources to provide three different complexity regimens. From d 0 to 7, a water-soluble globulin x diet complexity interaction (P < 0.05) was observed for average daily gain (ADG) and gain:feed (G/F). Increasing diet complexity increased ADG and G/F for pigs provided water, whereas the medium diet complexity regimen optimized performance for pigs offered water-soluble globulin. From d 0 to 14, pigs fed the two more complex regimens had greater ADG and G/F (P < 0.01) than the pigs fed the least complex regimen. Pigs offered water-soluble globulin had decreased (P < 0.01) ADFI, but increased (P < 0.001) G/F from d 0 to 14. For overall performance (d 0 to 35), increasing diet complexity increased (P < 0.03) ADG and ADFI, whereas water-soluble globulin offered from d 0 to 14 had no effect. In Exp. 2, 360 weanling pigs (5.2 +/- 1.6 kg; 19 +/- 4 d of age) were used in a 21-d growth assay. The trial was arranged as a 2 x 3 factorial with pigs fed the low- or medium-complexity diets (Exp. 1) with water or a 3% solution of water-soluble globulin offered for 4 or 8 d after weaning. From d 0 to 4, pigs offered water-soluble globulin had increased (P < 0.001) ADG and G/F compared with pigs provided water, whereas from d 4 to 8, pigs provided water had increased (P < 0.05) ADG and G/F compared with pigs offered water-soluble globulin. Pigs fed the medium-complexity diet had increased ADG and G/F (d 4 to 8 and d 8 to 12) compared with pigs fed the low-complexity diet. From d 0 to 8 and d 0 to 21, pigs provided water-soluble globulin for 4 or 8 d after weaning had improved G/F compared with pigs provided water. Results demonstrate that providing water-soluble globulin through the water source of weanling pigs improves ADG and G/F immediately after weaning.

Animal Feed↗

[Thermal analysis on water components in brain tissue--quantitative determination of free and bound water fractions].

In the living system, tissue water is considered to be composed of both free and bound water. Bound water encompasses the structural water of the cell wall and of various biological substances of high molecular weight, such as proteins and polypeptides. The present study was designed to measure thermoanalytically free and bound water on a quantitative basis in fresh brain of rats using differential scanning calorimetry (DSC). Our intention was to determine the fraction of freezable water in tissue. Freezable water in a tissue represents the fraction of free water. In the present study, freezing was conducted at a constant rate of -10 degrees C/min from room temperature to -75 degrees C by a SSC/560 S (Seiko Electronics). The system allows for calculation of the amount of free water from the differential scanning calorimetry curve employing a coloric constant of 79.4 cal/mg. Aluminium oxide was used as calorimetric reference. The fraction of bound water was calculated by subtraction of the amount of free water from that of total tissue water. Water binding to solid tissue component was estimated from tissue dry weight and the bound water fraction. Mean water content of normal gray matter in adult Wistar rats was 76.9 +/- 1.4% (SD). 88.9% of total tissue water was free whereas 11.1 +/- 2.8% (SD) was bound. Bound water of brain parenchyma amounted to 0.44 +/- 0.12 mg/mg dry weight. As compared to other tissues such as cardiac muscle and liver, brain parenchyma obviously exceeded in free water content. The total water content of serum was 94.4 +/- 1.2%; 90.7 +/- 2.6% was free and 9.3 +/- 2.6% (SD) was bound.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Estimation of water turnover rates of captive West Indian manatees (Trichechus manatus) held in fresh and salt water.

The ability of West Indian manatees (Trichechus manatus) to move between fresh and salt water raises the question of whether manatees drink salt water. Water turnover rates were estimated in captive West Indian manatees using the deuterium oxide dilution technique. Rates were quantified in animals using four experimental treatments: (1) held in fresh water and fed lettuce (N=4), (2) held in salt water and fed lettuce (N=2), (3) acutely exposed to salt water and fed lettuce (N=4), and (4) chronically exposed to salt water with limited access to fresh water and fed sea grass (N=5). Animals held in fresh water had the highest turnover rates (145+/-12 ml kg-1 day-1) (mean +/- s.e.m.). Animals acutely exposed to salt water decreased their turnover rate significantly when moved into salt water (from 124+/-15 to 65+/-15 ml kg-1 day-1) and subsequently increased their turnover rate upon re-entry to fresh water (146+/-19 ml kg-1 day-1). Manatees chronically exposed to salt water had significantly lower turnover rates (21+/-3 ml kg-1 day-1) compared with animals held in salt water and fed lettuce (45+/-3 ml kg-1 day-1). Manatees chronically exposed to salt water and fed sea grass had very low turnover rates compared with manatees held in salt water and fed lettuce, which is consistent with a lack of mariposia. Manatees in fresh water drank large volumes of water, which may make them susceptible to hyponatremia if access to a source of Na+ is not provided.

Animals↗

Physicochemical quality of drinking and hot waters in Finnish buildings originated from groundwater or surface water plants.

The physicochemical quality of drinking and hot waters of 67 buildings in different parts of Finland was studied. Some of the buildings used processed groundwater and some processed surface water. Drinking water samples were taken from the first tap after the water was led into the building. Hot water samples were taken from taps and showers and from circulating hot water systems. Thy physicochemical quality of drinking water was affected by the origin of raw water used in the water plants. Drinking water from surface water plants contained more organic matter and less metals than water from groundwater plants. The quality goal for total organic carbon (TOC; < 2 mg l-1) was exceeded by all drinking water samples. In groundwaters, the variation in the content of non-purgeable organic carbon (NPOC) was great, probably because artificial groundwaters processed from surface waters were included in this group. Unlike in natural waters, the correlation between KMnO4-number and NPOC in the processed waters was weak. This result shows that KMnO4-number is an inaccurate estimate for organic carbon in processed waters. Corrosion of pipe materials was seen as elevated concentrations of iron and copper. In general, the physicochemical quality of drinking and hot waters in the buildings was rather similar.

Carbon↗

[Drinking water data collection and information system in North Rhine-Westphalia. Integration of water supply zones].

To comply with the drinking water regulation act of 2001 and as basis for reports to the European Community, North Rhine-Westphalia has defined water supply zones and collected the necessary data from local health authorities. The software used for drinking water surveillance by the local health authorities and on state level has been adapted to the new requirements. In principle, a supply zone in NRW means a region where the water comes from one water-works. Under certain conditions exceptions are possible. 53 out of 54 local health authorities supplied data about water supply zones up to summer 2006. There are 410 water supply zones which get their water from around 550 water-works. The number of people per supply zone varies between a few hundred and nearly 900,000. Most of the supply zones fall within the responsibility of one health authority. Only 37 supply zones out of 410 contain regions of more than one local health authority. 27 % of the supply zones get water from three or more water-works, 73% get water from one or two water-works. Looking at the other side of the medal, the structure is also relatively simple. More than 80% of the water-works deliver water into only one supply zone. The definition of water supply zones as chosen in NRW has proved to be a good basis for a clear description of the drinking water supply. Besides, the definition enables the state to produce reports which can link water quality, regions and the supplied persons.

Database Management Systems↗

[Transpiration water consumption of young Platycladus orientalis and Robinia pseudoacacia trees and their correction functions under different water supply].

Through artificial water control in the shed of rain-free, this paper studied the physiological water requirement patterns of 2-3 years old Platycladus orientalis and Robinia pseudoacacia trees during their growth period, and the relationships between their transpiration water consumption and soil water supply. The results showed that the transpiration water consumption of Robinia pseudoacacia was increased with increasing soil water supply within the range of 40%-100% of field water-holding capacity. Its maximum transpiration water consumption was at the early and accelerating growth stages, accounted for 80.5% of total annual water consumption. The transpiration water consumption of Robinia pseudoacacia was 5.13 times as much as that of Platycladus orientalis. Platycladus orientalis had a peak value of transpiration water consumption when the soil moisture content was 40%-100% of field water-holding capacity. Its transpiration water consumption was the maximum at accelerating growth stage, accounted for 46.27% of total annual water consumption, next at later growth stage, and relatively small at early growth stage. The correction functions of transpiration water consumption to soil water supply and the time-soil moisture functions of practical transpiration water consumption under insufficient water supply for two test species were put forward for the first time.

Ecosystem↗

Effects of acute fresh water exposure on water flux rates and osmotic responses in Kemp's ridley sea turtles (Lepidochelys kempi).

Water flux rates and osmotic responses of Kemp's Ridley sea turtles (Lepidochelys kempi) acutely exposed to fresh water were quantified. Salt-water adapted turtles were exposed to fresh water for 4 d before being returned to salt water. During the initial salt water phase, absolute and relative water flux rates were 1.2+/-0.1 l d(-1) and 123.0+/-6.8 ml kg(-1) d(-1), respectively. When turtles were exposed to fresh water, rates increased by approximately 30%. Upon return to salt water, rates decreased to original levels. Plasma osmolality, Na(+), K(+), and Cl(-) decreased during exposure to fresh water, and subsequently increased during the return to salt water. The Na(+):K(+) ratio was elevated during the fresh water phase and subsequently decreased upon return to salt water. Aldosterone and corticosterone were not altered during exposure to fresh water. Elevated water flux rates during fresh water exposure reflected an increase in water consumption, resulting in a decrease in ionic and osmotic concentrations. The lack of a change in adrenocorticoids to acute fresh water exposure suggests that adrenal responsiveness to an hypo-osmotic environment may be delayed in marine turtles when compared to marine mammals.

Adaptation, Physiological↗

Water pricing towards sustainability of water resources: a case study in Beijing.

The role of water pricing for managing water resources is widely recognized in many areas of the world because of the increasing scarcity of water resources, a high competition between water uses and environmental degradation. Based on the analysis of cost of water, this paper explores which types of cost should be reflected in the water pricing enhancing the sustainability of water resources. The principle of full cost pricing in which the cost should include supply cost, opportunity cost and externalities is proposed as a means to achieve the sustainability of water resources. In a case study of Beijing, low water price is analyzed as one reason for unsustainable water consumption. Thus water pricing justified is necessary and pressing. It is proposed to justify water price in phased manner and eventually towards full cost pricing. The assessment of impacts on water resources by raising water price shows water pricing could alleviate the conflict between water supply and demand. This paper concludes that water pricing can play an effective role in enhancing the sustainability of water resources in Beijing.

China↗

Water, mineral waters and health.

The authors focus on water resources and the use of mineral waters in human nutrition, especially in the different stages of life, in physical activity and in the presence of some morbid conditions. Mineral water is characterized by its purity at source, its content in minerals, trace elements and other constituents, its conservation and its healing properties recognized by the Ministry of Health after clinical and pharmacological trials. Based on total salt content in grams after evaporation of 1l mineral water dried at 180 degrees C (dry residues), mineral waters can be classified as: waters with a very low mineral content, waters low in mineral content, waters with a medium mineral content, and strongly mineralized waters. Based on ion composition mineral waters can be classified as: bicarbonate waters, sulfate waters, sodium chloride or saltwater, sulfuric waters. Based on biological activity mineral waters can be classified as: diuretic waters, cathartic waters, waters with antiphlogistic properties. Instructions for use, doses, and current regulations are included.

Bicarbonates↗

Water management practices in rural and urban homes: a case study from Bangladesh on ingestion of polluted water.

Although Bangladesh has achieved remarkable success in extending the availability of hand pumped and piped water, unsafe water is still ingested. This brief study attempted to assess water management practices in rural and urban homes in Bangladesh so as to establish the routes by which unsafe water is ingested, to examine methods of collection and storage, and determine why unsafe water sources are used when unsafe supplies are available. Forty-eight rural and forty-five urban slum households were studied. Observations, interviews and water quality investigations were conducted. The results show that the respondents were aware that hand pump/tap water is safe and took care to use these safe sources for drinking purposes. However, they continued to use surface water for non-drinking activities such as bathing, washing and rinsing their mouths. Reasons were given that it was a traditional practice to bathe in surface water and was more enjoyable. One of the reasons given for not using hand pumped water to wash clothing and food was that such groundwater caused staining. Bacteriological results from such ingested water showed the quality, especially in rural areas, to be poor. Results also showed the internal surfaces of the base of storage containers to be heavily contaminated with bacteria. This showed that water that was safe when it was first drawn would became contaminated during storage. This study had a limited scope; much further research is needed to find what determines and how water becomes contaminated in containers. These factors include how to reduce contamination of water, in particular the relationship between growth of bacteria in stored water and the material from which the container is made and how to improve the overall quality of water. On the management side, studies could be conducted as to how to improve people's understanding of the use of water for domestic purposes and its safe management.

Adult↗

Remote site production of sterile purified water from available surface water.

A water purification and sterilization device was tested for its functional capabilities. Challenge water consisting of potable water augmented with bacteria, endotoxin, virus, suspended solids, and dissociable ions (sodium chloride, lead or arsenic salts) was passed through the device. The product water quality attributes were analyzed. The device demonstrated reduction in bacteria of >7 logs, endotoxin was reduced by >4 logs, virus was reduced by >4 logs, and dissociable ions were reduced by >3 logs. The product water of the device met the limits for a range of chemical entities specified by the United States Pharmacopeia and Association for the Advancement of Medical Instrumentation. The product water met the quality attributes of Sterile Water for Injection, USP, Sterile Purified Water, USP, and the Water for Dialysis. The device provides a logistical advantage in reducing the weight of transport of packaged water by 83% and the cube by 67%. It operates manually by gravity and is disposable after a single use. The device provides an effective alternative to the transport and use of packaged sterile water in remote locations by production of sterile water at the point-of-need using available water. It also is capable of producing safe drinking water following the production of clinical waters. This device has been cleared by the US Food and Drug Administration for production of three liters Sterile Purified Water, USP from Environmental Protection Agency (EPA) grade drinking water.

Communicable Disease Control↗