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Rapid atrophy of mouse soleus muscles after tenotomy depends on an intact innervation.

Changes in length and mass of mouse soleus muscles have been determined during the first 14 days after division of the Achilles tendon and/or the tibial nerve. Muscle atrophy and associated histological changes were detectable 24 h after tenotomy, and increased progressively over the first week. These changes were less marked in muscles which had also been denervated, and were rapidly reversed if the tendon became reattached. An attempt is made to distinguish the role of the nerve supply from the effects of reduced longitudinal tension in the production of atrophy after tenotomy.

Achilles Tendon↗

The fate of proliferating cells in skeletal muscle after denervation or tenotomy: an autoradiographic study.

During the first 7 days after denervation or tenotomy of skeletal muscle there is increased cell proliferation, as measured autoradiographically by [3H]thymidine incorporation. By 21 days most of the labelled cells have disappeared. The present experiment was designed to measure residual levels of labelling in skeletal muscles at 21 days after denervation or tenotomy, when [3H]thymidine had been available for the first 7 days. This was to determine the fate of cells labelled at 7 days and to detect whether continued cell proliferation had occurred during the intervening period, as evidenced by the dilution of nuclear label. Tibialis anterior muscles of 16 mice were denervated, tenotomized or sham-operated, and the animals injected with a single daily pulse of [3H]thymidine for the first 7 days only. In denervated muscle the 11% of muscle nuclei and 41% of connective tissue nuclei labelled at 7 days had decreased to 2 and 13%, respectively, by 21 days. These values were still significantly higher than in 21 day controls (0.2 and 1.9%, respectively). In muscle nuclei (myonuclei and satellite cell nuclei) there was a significant decrease in the amount of label per nucleus. This was interpreted as evidence of continued cell proliferation. However, this did not produce an increase in numbers of muscle nuclei. It was concluded that: there was turnover of satellite cells and possibly myonuclei, the latter probably through continued division and incorporation of satellite cells; and the extra satellite cells and myonuclei produced were probably extruded from the fibre to the extracellular space.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Tenotomy delays both synapse elimination and myogenesis in rat lateral gastrocnemius.

To investigate a possible relationship between synapse elimination and myogenesis, we examined both phenomena during the first 2 weeks of postnatal life in the rat lateral gastrocnemius muscle. Synapse elimination and myogenesis occur simultaneously. Sixty per cent of the number of fibers observed in adult muscles is generated during the first 10 days of postnatal life; during this time, the majority of muscle cells in lateral gastrocnemius also become singly innervated. We delayed synapse elimination by cutting the tendon of insertion of lateral gastrocnemius (tenotomy) on the day of birth. Both synapse elimination and postnatal myogenesis were slowed by tenotomy. Tenotomized muscles contained fewer detectable cells than unoperated contralateral control muscles. These results suggest that synapse elimination may be altered by altering postnatal muscle fiber addition.

Animals↗

Grave complications after superior oblique tenotomy or tenectomy for Brown syndrome.

INTRODUCTION: Superior oblique tenotomy or tenectomy was the preferred procedure for Brown syndrome for decades. Superior oblique palsy was reported as a complication, but the complex nature of this palsy and the difficulty in treating it was not emphasized. We documented cases with distressing symptoms of unilateral and bilateral superior oblique palsy after a free tenotomy or tenectomy of the superior oblique tendon for Brown syndrome. METHODS: Four cases referred for management of complex strabismus after superior oblique surgery for Brown syndrome were identified. Case histories, complications, and corresponding management are described in detail. RESULTS: All cases presented with bothersome symptomatic superior oblique palsy-incomitant vertical deviation with significant torsion, diplopia worse in functional down gaze, and anomalous head postures. Although reanastomoses of the superior oblique tendon was attempted in all cases,the procedure was modestly successful in only one case. Superior oblique palsy could not be reversed. After three procedures, Case 1 was orthotropic in primary, right, and left gaze but had a small intermittent tropia in down gaze. Case 2 underwent bilateral superior oblique operations. Despite a unilateral reanastomosed superior oblique tendon, hypertropia in down and left gaze and 15 degrees chin down position persisted. Case 3 showed correction of head tilt and a negative cover test 1 week after the second operation for iatrogenic superior oblique palsy, but stability of the procedure could not be ascertained. Case 4 had a successful attempt at reanastomosis and regained control of the vertical deviation as a hyperphoria. Both anomalous head posture and torsion improved. CONCLUSION: Superior oblique surgery for Brown syndrome may cause irreversible serious strabismus problems. Patients are left with distressing cyclovertical deviation worse in the functional position of gaze with significant torsional component that was resistant to therapy. Although adaptive mechanisms, such as an anomalous head posture, may develop, patients are left with a permanent disability that could not be reversed. One should scrupulously adhere to the indications for surgery in Brown syndrome. Preoperative assessment of superior oblique function is stressed. An alternative surgical procedure that is potentially reversible should be considered. This should include a reliable method to recover both ends of the tenotomized superior oblique tendon in case the procedure needs to be modified at a later date.

Child↗

Intramuscular tenotomy of flexor digitorum superficialis in the distal forearm after surgical excision of dupuytren's disease.

Contracture of the proximal interphalangeal joint after surgery to excise Dupuytren's disease, despite release of the contributory structures within the finger, can be caused by flexor digitorum superficialis (FDS) contracture. We describe five cases where FDS contracture was released by intramuscular tenotomy in the distal forearm. Standard postoperative therapy for Dupuytren's fasciectomy was used and clinical review showed improved finger extension with no loss of strength. We suggest that intramuscular tenotomy of FDS in the forearm can be used safely where indicated after excision of the Dupuytren's disease.

Dupuytren Contracture↗

[Subcutaneous tenotomy of the sternocleidomastoid muscle as therapy in muscular torticollis].

This study reviews the cases of 49 patients with congenital muscular torticollis after an average follow-up of seven years, who were treated with subcutaneous sternomastoid tenotomy. An excellent result was found in 18 patients (37%), a good result in another 18 patients (37%), while the result of 13 patients (26%) must be regarded as unsatisfactory. There were no severe complications to be seen. These results are compared with other operative technics of treatment of muscular torticollis and the poor results are analyzed. We believe that subcutaneous sternomastoid tenotomy is a good method for treatment of muscular torticollis, with the advantage of avoiding any ugly scar.

Adolescent↗

[Tenotomy of the superior oblique muscle in treatment of A-pattern strabismus and Brown syndrome].

The superior oblique tenotomy and tenectomy is an very effective and predictable surgical method in correcting the A-pattern in strabismus when overaction of one or both superior oblique muscles is present. In this series of 42 patients with an A-pattern an average correction of 86 to 91% was obtained regardless of the degree of the A-pattern. So we didn't observe any case that was converted from an A- to a serious V-pattern as obviously a less reduction is obtained in lesser degrees of A-pattern. In 11 cases of true but atypical Brown's-syndrome the tenotomy of the superior oblique muscle had a very beneficial effect on the limited movement of the affected eye and a co-existing abnormal head posture.

Child↗

Ultrasound-guided percutaneous longitudinal tenotomy for the management of patellar tendinopathy.

Thirty-eight athletes with unilateral patellar tendinopathy (17 with a tendinopathy of the main body of the tendon, and 21 with an insertional tendinopathy) underwent ultrasound-guided multiple percutaneous longitudinal tenotomy under local anaesthetic infiltration after failure of conservative management. Thirty-four patients were reviewed at least 24 months after the operation. Sixteen patients were rated excellent, nine good, eight fair, and five poor. Nine of the 13 patients with a fair or poor result had an insertional tendinopathy, and eight of them underwent a formal exploration of the patellar tendon. Before the operation, there were some areas of altered echogenicity at and around the site of involvement. These were still visible 6 wk after surgery in 70% of the patients. At the latest follow-up, in the patients with an excellent or good result, the tendon was generally isoechogenic but slightly thicker (P = 0.06) than the normal contralateral. In the patients with a fair or poor result, the tendon was significantly thicker than the contralateral (P = 0.03), and showed some areas of mixed echogenicity. In the patients in whom the procedure was successful, the thicker operated tendon did not interfere with physical training. Bilateral isokinetic peak torque (Nm), average work (Joules), and average power (Watts) were tested at 90 degrees x s(-1). Immediately before the operation, there was no significant difference in peak torque, but total work and average power were significantly lower in the limb to be operated (0.01 < P < 0.05). By the end of the study, although peak torque was, on average, within 7% of the unoperated limb, total work and average power were still significantly lower than in the unoperated limb (0.01 < P < 0.04). Percutaneous longitudinal internal tenotomy is simple, can be performed on an outpatient basis, requires minimal follow-up care, does not hinder further surgery should it be unsuccessful, and, in our experience, has produced no significant complications. In our hands, it has become the first line operative intervention in the treatment of chronic patellar tendinopathy after failure of conservative management. However, patients should be advised that, if they suffer from an tendinopathy at the attachment of the patellar tendon at the lower pole of the patella, a formal surgical exploration with stripping of the paratenon is preferable.

Adolescent↗

Fowler central slip tenotomy for old mallet deformity.

Fowler's central slip tenotomy for old mallet deformity is a well-known, but apparently seldom used procedure. Although the vast majority of patients with mallet finger can be successfully treated by well-known conservative techniques, occasional patients, such as the 11 reported here, will have enough difficulty with a chronic mallet finger to warrant rebalancing of the extensor apparatus by means of central slip tenotomy.

Adult↗

Adductor transfer versus tenotomy for stability of the hip in spastic cerebral palsy.

Two groups of children with cerebral palsy are compared with respect to the migration of the hip before and after 36 adductor transfers and 29 adductor tenotomies. The results in the two series are positive, with no significant difference (4 and 7%/year, respectively). Adductor transfer is a more stressful intervention for the child, with no proven advantage as yet. Therefore, we have for the time being withdrawn this procedure in favor of adductor tenotomy.

Adolescent↗

Percutaneous Achilles tendon lengthening complicated by inadvertent tenotomy.

Thirteen percent (five of 37) of all Hoke percutaneous triple hemisection Achilles tendon lengthenings performed at a teaching hospital in a 4-year period were complicated by inadvertent Achilles tenotomy. Three of the five patients had bilateral procedures and served as their own controls. After 2 months of postoperative short leg walking cast immobilization, the results in patients with inadvertent Achilles tenotomy were indistinguishable from those in whom tendon continuity was maintained.

Achilles Tendon↗

The myotubal origin of rat muscle fibres affects the extent of tenotomy-induced atrophy.

1. Immature muscle fibres (myotubes) can be divided into primary and secondary generations, which differ from each other in their time of formation, growth rates and myosin isoform expression. It is unclear whether the intrinsic differences between primary and secondary myotubes are totally extinguished once they mature and extrinsic factors, such as load, become important. 2. Four pregnant rats were injected with 5-bromo-2'-deoxyuridine (BrdU) on the 14th and 15th days of gestation. This selectively and permanently labels primary myotubes. Ten rats from four litters were killed when 8 months old, with three males (365-430 g) from a single litter being used for the quantitative study and the remainder being examined qualitatively. The extensor digitorum longus muscle (EDL) in each rat was tenotomized for 14 days. The sizes of fibres in the EDL were then correlated with their fibre type and whether they contained BrdU-labelled nuclei. 3. We reported that (i) II A and II B fibres derived from primary myotubes atrophied significantly less after tenotomy than II A and II B fibres derived from secondary myotubes and (ii) BrdU-labelled myonuclei were retained in the tenotomized muscle, even though tenotomy resulted in a substantial loss of myonuclei from the EDL. 4. We conclude that the origin of a fibre is a determinant of its response to the external forces which control its size, and hence force generation.

Adenosine Triphosphatases↗

The effects of tenotomy and overload on the postnatal development of muscle fibre histochemistry in the cat triceps surae.

Five to seven day-old kittens were subjected to partial tenotomy of the Achilles (triceps surae) tendon. The effects of tenotomy and overload on the development of muscle fibre histochemistry and fibre sizes were investigated when the cats had reached the adult stage. The examined muscles were the uniform soleus and the mixed medial gastrocnemius. Tenotomized muscles of both types had lower weights than their controls. Tenotomized soleus showed a redistribution of succinic dehydrogenase (SDH) activity, together with signs of muscle fibre death. Tenotomized medial gastrocnemius muscles displayed more severe degenerative signs than the soleus, together with signs of fibre death. The fibre death seemed to affect mainly fibres of type IIb. Moreover, a less distinct differentiation in histochemical staining pattern between muscle fibre types was found in these muscles. Overloaded soleus muscles had greater weights than their controls, while no difference could be shown for the overloaded medial gastrocnemius. The overloaded medial gastrocnemius showed a uniform hypertrophy of all fibre types. Also, overloaded soleus showed a uniform hypertrophy. Both types of muscle showed a normal histology as well as normal staining characteristics (SDH and AcATPase). It is concluded that both soleus and medial gastrocnemius are sensitive to loss of muscle tension during development. The basic features of muscle morphology and histochemistry were normal, though, and it is suggested that other factors account for most of the normally occurring development and differentiation.

Achilles Tendon↗

Axonal conduction velocity changes following muscle tenotomy or deafferentation during development in the rat.

1. The conduction velocities of axons supplying the intertransverse caudal muscles of 8-week-old rats were measured. The distribution of conduction velocities was found to be similar to the more commonly studied hind-limb innervation. 2. In animals in which the intertransverse caudal muscles had been tenotomized at birth, however, the conduction velocities attained by both the sensory and the motor nerves by 8 weeks of age were significantly reduced. 3. This effect is limited to growing animals since tenotomy of the intertransverse caudal muscles for the same period in adults had no effect on axonal conduction velocity. 4. Deafferentation of normal intertransverse caudal muscles during development also significantly reduced the conduction velocities attained by the motor innervation, to the same extent as tenotomy had done. 5. These results are discussed in relation to the role of impulse traffic in the development of neuronal dimensions.

Animals↗

Protein synthesis in adult skeletal muscle after tenotomy: responses to fasting and insulin infusion.

Muscle growth was established in specific muscles in the hindlimb of adult female rats by tenotomy of the gastrocnemius muscle. Seven days after surgery there was an increase in the wet weight of the soleus (Sol) and plantaris (P) muscles and a decrease in that of the gastrocnemius (G) muscle from the tenotomized limb compared with the respective control muscles from the contralateral limb from the same animal. In all three muscles there was a significant increase in the fractional rate of protein synthesis (ks) in the muscles from the tenotomized limb above the rate of the respective control muscles. In contrast, the extensor digitorum longus (EDL) muscle showed no change in wet weight or ks 7 days after tenotomy of G. Fasting for 12 or 36 h had no significant effect on ks in G, P, or Sol muscles from either the control or tenotomized limbs. In EDL from the control limb, both fasting periods resulted in a significant decrease in ks, although this effect was not seen in the EDL from the tenotomized limbs of the same animals. A subsequent 30-min insulin infusion was similarly ineffectual in G, P, and Sol, with its only effect evident in the EDL from the control limb, where it was sufficient to reverse the decreased ks resulting from the fasting, even though after 36 h fasting the reversal was only partial.

Aging↗

Tenotomy and repair of latissimus dorsi muscles in rats: implications for transposed muscle grafts.

The functional properties of latissimus dorsi (LTD) muscles were evaluated 160 to 180 days after tenotomy and repair, when grafts had stabilized. Our hypothesis was that, compared with control LTD muscles, LTD grafts would develop less absolute force and power but that the specific force and normalized power would not differ. Expressed as a percentage of the value for control LTD muscles, values for grafts were 67% for muscle mass, 74% for mean single fiber cross-sectional area, 56% for maximum absolute isometric tetanic force, 64% for maximum absolute average force during shortening, and 70% for maximum absolute power. Compared with control LTD muscles, grafts showed no significant differences either in the number of fibers in the total muscle cross section or in the optimum velocity for the development of power. When force and power of grafts were normalized for total fiber cross-sectional area and mass, respectively, only the value for maximum specific force (84% of control value) was significant. The mechanisms responsible for the decrease in specific force after tenotomy and repair are not known. In contrast to the deficit in maximum specific force, the 30% deficit in maximum absolute power of grafts compared with control LTD muscles was explained completely by the 33% smaller muscle mass.

Animals↗

Tenotomy of the adductor longus tendon in the treatment of chronic groin pain in athletes.

Eighteen tenotomies of the adductor longus tendon were performed in 16 consecutive male athletes (aged 20 to 42) as treatment for chronic groin pain. The criteria for surgery was a history of long-standing (range, 2.5 to 48 months) and distinct pain at the origin of the adductor longus muscle, refractory to conservative treatment. At followup 35 months (range, 4 to 84) after surgery, all patients were improved or free of symptoms. All but 1 of the athletes returned to the same sport within a mean of 6.6 weeks, and 12 of 16 returned to competitive sports within a mean of 14 weeks after surgery. A majority of the patients (10 of 16) returned to full athletic activity, whereas 5 of 16 performed at a reduced level. One patient discontinued his sports activity due to other causes. In conclusion, when conservative treatment fails, tenotomy of the adductor longus tendon gives good long-term functional results in the treatment of chronic groin pain that is localized at the origin of the adductor longus muscle. A decreased muscle strength was observed in this study and did not seem to influence participation in sports.

Adult↗

Results of percutaneous longitudinal tenotomy for Achilles tendinopathy in middle- and long-distance runners.

From August 1989 to January 1995 we performed multiple percutaneous longitudinal tenotomies under local anesthetic on 52 middle- and long-distance runners with unilateral Achilles tendinitis or peritendinitis that had failed conservative treatment. Forty-eight patients were reviewed at an average of 22.1 months (SD, 6.5) after surgery. Results were rated as excellent in 25 patients, good in 12, fair in 7, and poor in 4. Four patients developed subcutaneous hematomas. One patient developed a superficial infection at one of the incision sites, which was managed by oral antibiotics with full recovery. Three patients complained of over-sensitivity to the incisions; this was resolved by rubbing hand cream over the incisions several times a day. One patient developed hypertrophic painful scars on three of the five incisions, but corticosteroid injections yielded good functional and cosmetic results. Isometric strength and endurance of the gastrocsoleus complex was measured just before the procedure, and at 6 weeks and 6 months later. Both were within 10% of the normal contralateral limb by the 6th postoperative month. Percutaneous longitudinal tenotomy is simple, can be performed on an outpatient basis, requires minimal follow-up care, and, in our experience, has produced no significant complications. We use this procedure as the operative treatment of choice for cases of chronic tendinitis that have failed conservative treatment.

Achilles Tendon↗