Extracellular release of glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase from ram spermatozoa.
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Serum GOT, GPT, gamma-GT and GLDH were determined in 15 G-6-PD-deficient subjects during a hemolytic crisis and hemoglobinuria due to ingestion of fresh fava beans. The same G-6-PD-deficient subjects were studied again 2 months after the crisis, when they were asymptomatic. 15 normal healthy children served as controls. A statistically significant increase, above normal, in serum GOT, GLDH and gamma-GT was observed in the favic subjects during the crisis. All the values reverted to normal in the asymptomatic period.
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The results demonstrated that both acute and continuous administration of pentobarbital enhanced the CCl4-induced elevation of SGOT and SGPT activities. The potentiation of pentobarbital on CCl4-elevated SGOT and SGPT activities showed both time- and dose-dependent actions. The CCl4-induced elevation of both serum enzyme activities after continuous exposure of pentobarbital was still significant 6 days after the termination of pentobarbital. The CCl4-elevated SGOT and SGPT activities were also affected by various doses of Na-pentobarbital, although the degree of potentiation was less than the results obtained by pentobarbital pellet implantation. The present results further support the contention that proliferation of hepatic cells by barbiturates enhances CCl4-induced toxicity.
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With regard to adding Pyridoxal Phosphate (PALP), which combines with the active sites of AST and ALT, JSCC recommends measuring holoenzymes without adding PALP, reflecting the biological reaction. On the other hand, IFCC recommends the measurement of both holoenzymes and apoenzymes when PALP is added, reflecting the total diverted from the internal organs. It is important which recommendation to follow from a clinical point of view and from the viewpoint of reducing gaps in clinical facilities. Further, it is necessary to consider each isozyme as well as the time difference between apoenzyme and holoenzyme diversion from blood to accurately grasp the pathology and understand the measurement.
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