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At least 127 records · Page 7Linked to original sources
PubMed1980

Urinary tract infection caused by anaerobic bacteria in children.

Anaerobic bacteria were recovered from 5 children with urinary tract infection (UTI). Three had pyelonephritis and 2 cystitis. Two of the patients had a history of prior recurrent UTI. Urine samples were collected using suprapubic aspiration. The anaerobic organism recovered were 3 isolates of Bacteroides fragilis and one each of B. melaninogenicus, Peptococcus asaccharolyticus, and Bifidobacterium adolescentis. Mixed infection was present in 3 children. In 2 cases B. fragilis were present with Escherichia coli, and in the other case two anaerobes were present. All patients were treated with antimicrobial agents for ten to fourteen days and responded well to therapy. Two of the children had a recurrence of UTI with aerobic organisms recovered from their urine within six to eight months. This report demonstrates the association of anaerobic organisms with UTI in children. It is suggested that cultures for anaerobic organisms be performed in symptomatic children whose aerobic cultures show no bacterial growth.

Actinomycetales↗
PubMed1986

Peptococcaceae bacteremia.

Bacteremia with Peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. A 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. Eight of the 12 patients were young women hospitalized on the obstetrical service. Seven patients had postpartum endometritis and one patient had chorioamnionitis. The remaining four patients include a single patient each with pylephlebitis and microabscesses of the liver, ascending cholangitis, thoracic empyema, and necrotizing soft-tissue infection. Bacteriologic identification of the microorganisms revealed the following: Peptostreptococcus micros (5 patients), Peptostreptococcus asaccharolyticus (5 patients), Peptostreptococcus magnus (1 patient), and Peptococcus species (1 patient). Eleven of the 12 patients received appropriate antibiotic therapy. All patients did well and there were no major sequelae.

Adolescent↗
PubMed2005

Microbial analysis of the autogenous bone collected by bone filter during oral surgery: a clinical study.

PURPOSE: The purposes of the study presented are to identify the microbial content of the collected bone debris and to determine the antibacterial efficiency of chlorhexidine mouth rinse in reducing the microbial content of the collected bone debris. PATIENTS AND METHODS: Twenty-five patients who had asymptomatic fully impacted mandibular third molars bilaterally underwent surgical removal for prophylactic and orthodontic considerations. Immediately before surgery all patients rinsed with 10 mL of their assigned solution (chlorhexidine or sterile saline) for 2 minutes. Before surgical removal of the teeth, bone debris was collected with a stringent aspiration protocol from the ramus by bone filter. The microbial content of the bone debris was assessed and the bacterial levels between the 2 groups were compared statistically. RESULTS: All samples from both groups (chlorhexidine or sterile saline) yielded viable microorganisms. There was no significant difference between the mean/median colony-forming units (CFU)/g values of both group samples, for aerobes (Streptococcus salivarius) and anaerobes (Bacteroides, Peptococcus, Peptostreptococcus, and Veillonella species). With regard to total microorganisms, the mean CFU/g derived from the chlorhexidine group samples were 1.5 x 10(8) CFU/g per bone sample compared with 1.5 x 10(9) CFU/g for the sterile saline control group (P < .05). CONCLUSION: Although chlorhexidine rinsing immediately before surgery reduced the levels of total microorganisms when compared with stringent aspiration protocol alone, it has not been found effective on aerobic Streptococcus salivarius and, importantly, on anaerobes. The reduced bacterial levels may still carry high infectious risk and may lead to failure in autogenous grafting procedures in oral surgery.

Adolescent↗
PubMed1978

Aerobic and anaerobic bacteriology of acute otitis media in children.

Tympanocentesis and aerobic and anaerobic cultivation of the middle-ear fluid obtained was performed through one or both tympanic membranes of 62 children with acute otitis media. Aerobic bacteria alone, predominantely pneumococcus and Hemophilus influenzae, were isolated from 57% of patients; anaerobic organisms alone, most commonly Propionibacterium acnes and Peptococcus, from 15%. Thirteen percent yielded mixtures of aerobes and anaerobes, and several had multiple aerobic or anaerobic agents. The isolation of only anaerobic bacteria, supported in some patients by gram-stain observations of middle-ear fluid, suggest that these bacteria, especially Petococcus, may occasionally play a direct role in acute otitis media.

Acute Disease↗
PubMed1980

Peptococcla urinary tract infection with bacteremia: a case report.

The second documented case of urinary tract infection with associated bacteremia of Peptococcus asaccharolyticus origin is reported. High fever and low peripheral white blood count were manifestations of bacteremia with Pepococcus asaccharolyticus, an anaerobic gram-positive coccus. Although anaerobes have been documented to cause urinary tract infections resulting in bacteremia most previous reports implicate aerobes in these processes. The problems inherent in culturing urine for anaerobes are reviewed.

Aged↗
PubMed1979

[Association of acquired polyagglutinabilities of types T and B. An observation].

Although T and acquired B polyagglutinabilities are not exceptional, simultaneous occurence of the two types is a rarer phenomenon. Ten days after an open heart surgery operation, the patient, age 55, had an infectious syndrom : two strains, Clostridium perfringens and Peptococcus variabilis were isolated. Simultaneously, her red cells were found to be polyagglutinable. The association of T and acquired B polyagglutinabilities could be demonstrated by serological studies of the patient's red cells, and by in vitro transformation of normal red cells using culture supernatants obtained from the two isolated strains.

ABO Blood-Group System↗
PubMed1982

[Anaerobic microflora of praepubertal children (author's transl)].

Bacteriologic cultures of the vagina and the cervix of 38 praepubertal children, aged 3 months to 16 years (average age 9 years) from the outpatient department for infants and juveniles of the 1st Department of Obstetrics and Gynecology of the University of Vienna were obtained vaginascopically using anaerobic transportmedia (Port a Cul) and were examined for aerobic and anaerobic bacteria. An average of 5.3 species was also isolated per child (2.9 aerobic and 2.5 anaerobic species). The commonest anaerobes were Peptococcus, Peptostreptococcus, Bact. melaninogenicus, Proprionibacterium and Veillonella parvula, the prevalent aerobes, were Enterococci, E coli and Staphylococcus epidermidis. Our results suggest that anaerobic bacteria amount a significant component of the flora of the lower genital-tract of healthy children, as well as of infants with vaginal discharge.

Adolescent↗
PubMed1980

Anaerobic bacteria in nonspecific vaginitis.

To study the cause of nonspecific vaginitis, we analyzed vaginal fluid from normal women and from 53 women with nonspecific vaginitis, using quantitative anaerobic cultures and gas-liquid chromatography for short-chained organic-acid metabolites of the microbial flora. In normal vaginal fluid, lactate was the predominant acid, and the predominant organisms were lactobacillus and streptococcus species (lactate producers). In nonspecific vaginitis, lactate was decreased, whereas succinate, acetate, butyrate, and propionate were increased, the predominant flora included Gardnerella (Haemophilus) vaginalis (acetate producer), and anaerobes, which included bacteroides species (succinate producers) and peptococcus species (butyrate and acetate producers). After metronidazole therapy, symptoms and signs of nonspecific vaginitis cleared, butyrate and propionate disappeared, and lactate and lactate-producing organisms became predominant. We conclude that certain anaerobes act with G. vaginalis as causes of nonspecific vaginitis, and that a high ratio of succinate to lactate in vaginal fluid is a useful indicator in the diagnosis of this condition.

Adult↗
PubMedDate not supplied

Recovery of anaerobic bacteria from vented blood-culture bottles.

From January 1979, to July 1981, blood from each patient suspected of having bacteremia was collected in two bottles of commercially prepared tryptic soy broth; one bottle was vented for 24 hr to the atmosphere. Bottles were incubated at 37 degrees C for a maximum of seven days and were examined daily for signs of growth. Blind subcultures onto chocolate-agar plates were performed 18-24 hr after collection and again at 48 hr. For 63,106 bottles inoculated, a total of 4,788 strains were isolated, for a rate of 6.4% (excluding contaminants). Two hundred and thirty (4.8%) isolates were anaerobes, of which 75 (33%) were identified as Bacteroides fragilis. It is significant that 20 of these anaerobic isolates (approximately 9%) were recovered only from the vented bottles. These included eight strains of B. fragilis; three strains of Clostridium perfringens; and one strain each of Bacteroides distasonis, Fusobacterium nucleatum, Peptococcus saccharolyticus, and Eubacterium species. These results emphasize the need to check vented bottles for anaerobes, since significant numbers of these bacteria could be missed by failing to do so. These data further show the desirability of holding anaerobic blood cultures for a minimum of one week.

Bacteria, Anaerobic↗
PubMed1975

Susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli.

Susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. Only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. Considerable variation in susceptibility was observed among different genera and species of bacteria. Fusobacterium was most senstitive, followed by Clostridium, Bacteroides and Peptococcus, Peptostreptococcus, Veillonella and Acidaminococcus, and non-spore-forming gram-positive bacilli. Bacteroides fragilis was more sensitive than other species of Bacteroides. Similarly, Clostridium perfringens was more susceptible than other species of Clostriduim. While metronidazole appears to be a promising antimicrobial agent for infections caused by Fusobacterium, Clostrididium, and B.fragilis, therapy for infections with other anaerobic bacteria should be guided by in vitro tests of sensitiivity.

Anaerobiosis↗
PubMed1988

European collaborative study of reproducibility of quantitative sensitivity testing of anaerobes.

Minimum inhibitory concentrations (MICs) of ampicillin, cefoxitin, cefbuperazone, latamoxef, metronidazole, clindamycin and chloramphenicol were determined for 15 different anaerobic bacteria including Bacteroides spp., anaerobic cocci and Clostridium spp., in 18 European laboratories, who used their own methodology. The degree of intra- and inter-laboratory reproducibility was surprisingly good--87% of results fell on the modal MIC or were within one dilution of it and only 4.4% of the results differed by three or more dilutions. Results for clindamycin were the least reproducible, as were those for clostridia. Of the organisms that we tested Bacteroides fragilis ATCC 25285, NCTC 9343 emerged as the most suitable for use in quality control, and Peptococcus variabilis ATCC 14956 the most appropriate if a control for more slowly-growing species is required.

Ampicillin↗
PubMed1975

Effects of high risk and low risk diets for colon carcinogenesis on fecal microflora and steroids in man.

We investigated the effects of a high meat mixed Western diet and a nonmeat diet, representing the dietary pattern of high and low risk areas for colon cancer, respectively, on fecal microflora dn on bile acid and neutral sterol patterns in man. The total anaerobic microflora as well as the count of Bacteroides, Bifidobacterium, Peptococcus, and anaerobic Lactobacillus were significantly higher during the period of consumption of a high meat mixed Western diet comparted with the nonmeat-diet consumption period. The difference in total fecal bile acid excretion was not significant between the two dietary periods. Fecal excretion of microbially modified bile acids and neutral sterols was decreased when subjects eating a high meat diet transferred to a nonmeat diet. These results support the fact that diet plays a modifying role on the composition of intestinal microflora, bile acids, and neutral sterols.

Adult↗
PubMed1986

Quantitative bacteriology of acute dento-alveolar abscesses.

A qualitative and quantitative bacteriological study was performed on pus specimens obtained by needle aspiration of 50 acute dento-alveolar abscesses. Most samples contained a mixture of species (average 3.3); 20 (40%) of the abscesses contained anaerobes alone, 3 (6%) contained facultative anaerobes only and the remaining 27 (54%) contained mixtures of both types of bacteria, with anaerobes predominating. In total, 166 bacterial strains were isolated, 75% of which were strictly anaerobic; the most common species were Peptococcus spp, Bacteroides oralis and B. melaninogenicus. Among facultative anaerobes, Streptococcus milleri was particularly common. The mean concentration of bacteria in each abscess was 10(6.9 +/- 0.2). The mean concentration of anaerobic bacteria was 10(6.2 +/- 0.1) and of facultatively anaerobic bacteria 10(5.7 +/- 0.2).

Adult↗
PubMed1982

Eight year persistence of individual differences in the bacterial flora of the forehead.

Eight subjects having a rich (greater than or equal to 100,000 per cm2) population of Propionibacterium acnes on the forehead and 7 with a sparse population (great than or equal to 15,000 per cm2) in earlier tests were reexamined after median interval of 8 yr. All of those with an initial rich population had greater than or equal to 100,000 per cm2 on retest. Of the 7 with an initial sparse population, 4 again had fewer than 15,000, one had more than 100,000 per cm2 and 2 were intermediate. Three subjects harbored Peptococcus saccharolyticus in the initial test (26,000 to 75,000 per cm2) and all 3 carried this organism on the later test (5,000 to 49,000 per cm2). Of the 12 without P. saccharolyticus initially, 11 were again negative and one had a small number (200 per cm 2). We conclude that the presence or absence of P. saccharolyticus and the relative population density of P. acnes were individual characteristics that persisted in most subjects for at least 8 yr.

Adult↗
PubMed1991

Studies on the possible role of cattle nuisance flies, especially Hydrotaea irritans, in the transmission of summer mastitis in Denmark.

The summer mastitis pathogens Actinomyces pyogenes, Peptococcus indolicus, Bacteroides melaninogenicus ss. levii, Fusobacterium necrophorum and Streptococcus dysgalactiae were isolated from the polyphagous symbovine dipterans Hydrotaea irritans (Fallén) and Morellia sp. caught around dairy heifers on pasture, but not from the haematophagous species Haematobia irritans (L.), Haematobosca stimulans (Meigen), Culicoides sp. and Simulium sp. Secretions from clinical cases of summer mastitis proved to be sources of summer mastitis bacteria for more than 3 weeks despite antibiotic treatment and teat amputation. Taking into account the seasonal activity pattern of Hydrotaea irritans and its topographical distribution on grazing cattle, it appears evident that this fly may play a central role in the establishment and maintenance of the bacterial contamination with summer mastitis pathogens on the teats of healthy cattle. In the present study the survival of A.pyogenes and P.indolicus for 7 days in experimentally infected Hydrotaea irritans, as demonstrated by the recovery of these microorganisms from agar plates exposed to live infected flies, is described. However, experimental transmission of summer mastitis from sick to healthy heifers by Hydrotaea irritans proved unsuccessful.

Actinomyces↗
PubMed1999

Fungus-like hyphochytrids associated with human disease.

We report two cases, with liver and brain abscess, respectively, where fungus-like organisms belonging to the Hyphochytriomycota were found at the site of inflammation together with Peptococcus in the first and Cysticercus cellulosae in the second case. This is the first time these groups of organisms have been reported in human material. The role of hyphochytrids in human pathology remained uncertain as they were found together with already known human pathogens.

Adult↗
PubMed1979

Selective antibacterial action of 2-mercaptoethanol on propionibacteria in skin cultures.

2-Mercaptoethanol applied to the surface of agar medium had a selective antibacterial effect on Propionibacterium acnes and Propionibacterium granulosum without interfering with the growth of Peptococcus saccharolyticus or staphylococci in anaerobic cultures of skin or in pure cultures. In aerobic broth culture, 2-mercaptoethanol inhibited aerobes and stimulated anaerobes, consistent with its action as a reducing agent.

Aerobiosis↗