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At least 127 records · Page 7Linked to original sources
PubMed1963

DIRECT AND INDIRECT FLUORESCENT-ANTIBODY TECHNIQUES FOR THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA GROUP OF AGENTS.

Ross, Martin R. (Connecticut State Department of Health, Hartford) and Earle K. Borman. Direct and indirect fluorescent-antibody techniques for the psittacosis-lymphogranuloma venereum-trachoma group of agents. J. Bacteriol. 85:851-858. 1963.-Direct and indirect fluorescent-antibody (FA) techniques were developed for the detection of group antigen in infected tissue cultures and the titration of group antibody in human antiserum. The growth of the agent of meningopneumonitis (MP) in mouse embryo lung cell monolayers was followed by infectivity and complement-fixing (CF) antigen titrations, and cytological examination of FA stained cultures. Although infectivity and CF antigen reached a peak at 2 days and remained constant for an additional 3 days, only cells tested 2 to 3 days after infection were suitable for FA staining with labeled anti-MP serum because of excessive artifacts in the older cultures. Fluorescein isothiocyanate-labeled rooster and guinea pig anti-MP serums and human antipsittacosis serums were titrated in direct FA and hemagglutination-inhibition (HI) tests. The rooster conjugate showed brighter staining and higher antibody titers than the guinea pig or human conjugates and was more effective in detecting minimal amounts of virus antigen. FA staining reactions with 1 and 2 units of labeled rooster serum were inhibited by unlabeled rooster serum but clear-cut inhibition with human antipsittacosis serum could not be demonstrated. The indirect FA technique was successfully used for the titration of group antibody in human serum. A comparison of the indirect FA, HI, and CF tests showed the indirect FA technique to be intermediate in sensitivity between the HI and CF tests. None of the three tests showed significant cross reactions with human serums reactive for influenza A and B; parainfluenza 1, 2, and 3; respiratory syncytial virus; Q fever; or the primary atypical pneumonia agent.

Animals↗
PubMed1965

ENZYMES OF GLUCOSE CATABOLISM IN A MEMBER OF THE PSITTACOSIS GROUP.

Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Grisso, and Robert R. Brubaker. Enzymes of glucose catabolism in a member of the psittacosis group. J. Bacteriol. 89:810-812. 1965.-Extracts of preparations of the agent of meningopneumonitis made from infected chick-embryo allantoic fluid contained three enzymes of the pentose pathway of glucose degradation: glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and phosphoglucose isomerase. Vertical starch-gel electrophoresis showed that the two dehydrogenases were qualitatively different from the corresponding enzymes of the host. Enzymes of the Embden-Meyerhof and Entner-Doudoroff pathways were not found.

Animals↗
PubMed1997

Application of a nested, multiplex PCR to psittacosis outbreaks.

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR was genus specific, and the second-step PCR was multiplexed (i.e., had multiple primer sets in the same tube) and could discriminate among Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia trachomatis on the basis of the molecular weight of the amplicon. The limit of detection of each of the two PCR steps was 5 inclusion-forming units. We used PCR and serologic evidence during outbreaks of psittacosis to infer that C. psittaci had been transmitted from birds purchased in pet stores to humans. We also used this method to test both live and dead birds from pet stores for infection with C. psittaci. Compared with culture, the application of PCR to avian specimens increased the rate of C. psittaci detection.

Animals↗
PubMed1989

As sick as a pigeon--psittacosis myelitis.

An association between acute transverse myelitis and psittacosis in a teenage boy is described. Closer collaboration between doctors and vets might have made the diagnosis sooner. A full paediatric history should include details of contact with pets and other animals.

Acute Disease↗
PubMed1979

Pericardial effusion complicating psittacosis infection.

A 13-year-old schoolboy developed a moderately large pericardial effusion during the course of infection with psittacosis. The value of echocardiography in differentiating between pericardial effusion and myocarditis is shown.

Adolescent↗
PubMed1988

A progressive pericardial effusion caused by psittacosis.

A patient developed signs of a progressive pericardial effusion over a period of eight days. Diagnostic and therapeutic pericardiocentesis was performed. Viral titres to psittacosis rose from less than 1:8 to greater than 1:256 indicating recent infection.

Acute Disease↗
PubMed1991

Ovine psittacosis and sarcoidosis in a pregnant woman.

A woman in the first trimester of pregnancy presented with pneumonia and hilar lymphadenopathy after exposure to lambing ewes. She subsequently aborted. Infection with Chlamydia psittaci of ovine origin was confirmed. Pregnant women are susceptible to this infection, which may cause life threatening disease. The patient also had features of sarcoidosis, and the two conditions ran a similar time course. There is a possibility that ovine psittacosis caused an illness indistinguishable from sarcoidosis.

Abortion, Spontaneous↗
PubMed2002

Familial cases of psittacosis: possible person-to-person transmission.

A 29-year-old woman with primary myelofibrosis developed severe pneumonia, and 20 days later her 31-year-old sister also contracted pneumonia. The first patient had been in contact with parakeets but the second patient had not been in contact with any birds. Psittacosis was diagnosed in both cases by microplate immunofluorescence antibody technique. Person-to-person transmission between the sisters was suspected to have taken place.

Adult↗
PubMed1997

Nuclear bodies of the human lung in psittacosis.

Lung piece obtained from a female patient with psittacosis by biopsy. A nuclear bodies of five different types were observed, depending on their degree of ultrastructural development. First type showed fibrillary nuclear bodies surrounded by a clear halo. Type II resembled the nuclear body of type I but showed heterogeneously electron density. Type III comprised nuclear bodies with a fibrillary cortex containing a central granular component. These bodies were separated from the nucleoplasm by a clear halo. Type IV was characterized by a more complex structure and resembled the nuclear body of type III but with dense osmiophilic granules. Nuclear bodies of type V were comprised of numerous osmiophilic granules.

Cell Nucleus↗
PubMed1995

Severe psittacosis during pregnancy and suppression of antibody response with early therapy.

A cluster of 3 cases of psittacosis occurred among members of one family. The initial case was a pregnant woman who presented with extensive multilobar infiltrates and severe respiratory distress necessitating mechanical ventilation. Her respiratory failure worsened during 36 h of erythromycin therapy. Improvement coincided with the termination of pregnancy and the initiation of doxycycline treatment. The remaining patients developed milder illness. Serologic assessment of the affected subjects suggests that early treatment may suppress antibody response.

Adolescent↗
PubMed1997

Psittacosis. Is contact with birds causing your patient's pneumonia?

Psittacosis is an uncommon source of community-acquired pneumonia but can cause significant morbidity and possibly death if not properly diagnosed and treated. Although it occurs most often among handlers of pet psittacine birds, it can be contracted during poultry processing and other contacts with nonpsittacine birds. Clinical presentation is nonspecific, and history taking is often critical in establishing the diagnosis. All suspected cases should be confirmed with serologic antibody testing. Whenever possible, the source of the infection should be identified to prevent further spread of the illness. Infections in humans should be reported to the CDC.

Animals↗
PubMedDate not supplied

[A clinical case of hepatic psittacosis with ultrasonography anomalies of the liver].

Psittacosis marked by liver and spleen involvement and minimal pericarditis was observed in an 18-year-old patient hospitalized for fever of 1 month duration. Liver tests revealed cholestasis. Ultrasonography revealed multiple nodular formations in the liver and spleen, images confirmed on computed tomography. Liver biopsy showed granulomatous with hyperplasia of the Kupffer cells surrounded by healthy tissue. The diagnosis was confirmed by complement fixation. Treatment with tetracycline led to rapid remission of the fever and normalization of the liver tests and hepatic images.

Adolescent↗
PubMed1992

[Familial outbreak of pneumonia by psittacosis].

A familial outbreak (4 cases) of pneumonia by psittacosis in Vall de Uxó (Castellón) during the month of April in 1990 is described. In addition to the epidemiologic, clinical and serologic (complement fixation) studies carried out a survey and serology of 11 healthy controls (neighbors, and employees from a bird shop) were performed. The serum of a parrot recently acquired by the patients (positive at 1/8) was analyzed. The habit of petting the animal was significantly associated with the disease. The patient's response to treatment was good. The titers of antibodies versus Chlamydia psittaci were higher than those of the controls examined and the established threshold for positivity (1/36). In addition to clinical intervention the roles of epidemiology and the participation of veterinary services are fundamental to control this disease the exact prevalence of which is unknown.

Adult↗
PubMed1989

[Psittacosis: report of 7 cases].

The development and systematic use of microserological techniques have allowed to identify the etiology of a higher number of respiratory diseases, that could hardly have been considered some years ago and that now are known to play a more common role in clinical practice than usually thought. In the present study, the clinical, radiological and epidemiological features of seven patients are reported; they were seen in our health center during a 16 month period and the diagnosis of Chlamydia psittaci pneumonia was made by serological studies. All patients were treated with erithromycin, with favorable clinical and radiological outcome. Psittacosis is a condition that should be considered as a cause of community acquired pneumonia even in the absence of suggestive epidemiological background.

Adult↗
PubMed1989

Psittacosis pneumonia.

A 57-year-old man with symptoms and signs consistent with atypical pneumonia had epidemiologic and later serologic evidence of psittacosis pneumonia. Therapy with tetracycline resulted in rapid resolution.

Ciprofloxacin↗
PubMed1986

[Hepatic manifestations of psittacosis].

A moderate increase in transaminases and alkaline phosphatase serum concentrations was observed in 14 of 32 patients with psittacosis (antibodies anti-Chlamydia psittaci greater than or equal to 1/40 with the complement fixation test). Clinical manifestations were present in 4 patients (hepatomegaly in 4 cases; jaundice in one case). In one patient, histological examination of a liver specimen showed granuloma. In 13 patients treated with tetracycline or macrolides, the outcome was favorable. One patient recovered spontaneously. In comparison with the group of 18 patients without hepatic involvement, there was no difference pertaining to epidemiological, clinical, or serological findings. Equally, there was no difference in the course of the disease.

Adult↗
PubMedDate not supplied

Chlamydia immunoreactivity in birds with psittacosis: localization of chlamydiae by the peroxidase-antiperoxidase method.

Diagnosis of psittacosis (infection with Chlamydia psittaci) has traditionally been dependent upon culture or demonstration of chlamydiae in tissues by cytochemical stains such as the Gimenez or Macchiavello's stain. In this study, a peroxidase-antiperoxidase (PAP) technique that employs a commercially available monoclonal antibody directed against Chlamydia is used to demonstrate chlamydiae in the necropsy tissues of nine birds. Chlamydiae were identified in air sacs (83%), liver (78%), spleen (78%), small intestines (67%), large intestines (44%), and kidneys (33%). Usually chlamydiae were associated with microscopic lesions, but in 75% of large intestines examined, organisms were identified within the lumen of histologically normal intestine. PAP studies revealed numerous chlamydiae that were inapparent in hematoxylin-and-eosin-stained sections. These results demonstrate the utility and sensitivity of the PAP method in the identification of chlamydiae in microscopic sections.

Animals↗
PubMed1970

Psittacosis in Hamilton: a case report and epidemiological study.

A case report of a patient severely ill with psittacosis is presented, together with an epidemiological study of his fellow workers. This study illustrates the contagiousness of the disease and indicates that it is probably more prevalent than clinical experience suggests. Only three of the 83 persons studied who were exposed became ill enough to seek medical attention. It is important that both the physician and the public be made aware of this disease so that definitive treatment can be instituted as early as possible. Tetracycline is the drug of choice. Since this infection is widespread among many varieties of birds, and is not confined to those of the parrot family, this disease must be considered in any patient with an atypical pneumonia, especially when there has been contact with a bird. Since there is not likely to be a reinstitution of the ban on the importation of psittacine birds as domestic pets, the only alternative means of controlling outbreaks of this disease is public and physician awareness. Since some outbreaks appear to be spread from man to man, sometimes with high mortality, it is important that any patient suspected of having this disease be kept in strict isolation.

Adult↗