Mermithid parasite of Glossina brevipalpis Newstead.
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Penetrating infective juveniles of Romanomermis culicivorax usually killed first-stage larvae of Chironomus maturus Johann., Chironomus sp., Simulium damnosum Theo., and S. venustum Say. Nematodes were melanized and died after they entered fourth stage larvae of 2 chironomid species, but no host reaction was evident after entry into fourth-stage blackfly larvae. In contrast, the nematodes initiated development in the latter hosts, which died before the nematodes completed their development.
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A new genus and a new species Imosmermis morosovi gen. sp. n. found in the flea Xenopsilla gerbilli minor Jordan are described. The genus Imosmermis differs from all known genera of Mermithidae by the absence of mouth and reduction of stihostome and the dorsal chord. Body is long and very thin. A new genus Aphanimermis comb. n. for the species Psyllomermis tshumacovae Rubzov is created. This genus is characterized with a stilet, with high developed lateral and ventral chord as well as with a tail which is absent in other genera infecting fleas.
Mermithid parasites (Nematoda: Mermithidae) were observed in 11 mosquito species in 22 counties of Indiana, Michigan, Minnesota, Ohio and Wisconsin. Natural hosts included adult Aedes vexans, Ae. canadensis, Ae. cinereus, Ae. diantaeus, Ae. punctor, Anopheles punctipennis and Coquillettidia perturbans and larvae of Ae. abserratus, Ae. canadensis, Ae. cinereus, Ae. communis, Ae. diantaeus, Ae. provocans, Ae. punctor, Ae. stimulans and Cq. perturbans. These are the first records of such parasites in Indiana, Minnesota, Ohio and Wisconsin.
Field tests were carried out with the nematode parasite Romanomermis iyengari (Welch, 1964) to fight 3 species of mosquito larvae Anopheles albimanus (Wiedeman, 1821); Culex nigripalpus (Theobald, 1901); and Culex quinquefasciatus (Say, 1823) in three ecologically different natural mosquito reservoirs. The release of the nematode embryos (infective stage) in the above-mentioned reservoirs was carried out with a Holder-planta 5 manual sprayer, at 2 atm, and an application dose of 1000 parasite embryos/m2. The parasite embryos were obtained from 6 nematode cultures, soaked in distilled water. Results showed that mosquito larvae were parasitized by R. iyengari as follows: 100% of the A. albimanus and 85% of the C. nigripalpus, in the first reservoir; 85% of the C. nigripalpus, in the second reservoir; and 80% of the C. nigripalpus and 75% of the C. quinquefasciatus in the third one. Thus, the potential use of the nematode parasite Romanomermis iyengari as a biological control agent of mosquito larvae was shown.
The oviposition pattern of Romanomermis iyengari (Nematoda: Mermithidae) in relation to the moisture level in the soil was studied by seeding postparasites into two sets of 12 cm soil columns. While one set of the soil column was allowed to dry over a period of 60 days, the other set was maintained at a constant level of 15-20% moisture throughout the soil column. In the soil column maintained at constant moisture level, the postparasites oviposited primarily in the upper 3 cm layer (80-97%). In the soil column which was allowed to dry the percentages of eggs laid in the upper 3 cm layer were 96, 24 and 6 respectively on D20, D30 and D40 after seeding and the moisture levels on respective days were 12, 5 and 3%. On corresponding days the postparasites laid 1, 56 and 42% of the eggs in the lower most layer (9-12 cm) and the moisture levels were respectively 15, 10 and 9%. The results indicate that the adult nematodes migrated towards the bottom layer of the soil and laid eggs as the moisture of the upper layers decreased.
Isoenzyme electrophoretic patterns of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), esterase (EST) and peroxidase (PO) of post-parasitic juveniles and adults of Romanomermis yunanensis and R. culicivorax were analysed by disc electrophoresis, respectively. The protein patterns of the two species of Romanomermis were compared by vertical slab SDS polyacrylamide gel electrophoresis. The results showed marked differences in isoenzyme patterns (number of bands and/or Rf) of LDH and MDH between the two species of Romanomermis, but the similar positions of isoenzyme bands denoted a certain genetic relationship between them. No band was found in the isoenzyme patterns of PO and EST. Distinct differences in protein pattern were observed between the two species of Romanomermis, but some common bands in pattern reflected the phylogenetic relationships of these species. The differences of isoenzyme and protein patterns observed in this study have provided the reason for identifying and differentiating the two species of Romanomermis at the molecular level.