Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Library Associations”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 127 records · Page 7Linked to original sources
PubMed2002

Construction and preliminary screening of a human phage single-chain antibody library associated with gastric cancer.

BACKGROUND: The aim of this study was to construct a phage library of human single-chain antibodies associated with gastric cancer and screen such a library for CEA binding scFv. MATERIALS AND METHODS: The cDNA library of antibody variable regions was constructed using mRNA from metastatic lymph nodes or spleen of patients with stomach cancer by RT-PCR. These cDNA were assembled into a single-chain format and cloned into phagemid pCANTAB-5 and then transformed into Escherichia coli TG1. The scFv gene library was rescued by M13KO7 helper phage. CEA and the viable CEA-positive gastric cancer cell line MKN-28 were used to screen the phage antibody library. Indirect and tumor cell ELISA was used to determine the specificity of phage antibody. Fixed cell immunofluorescence and live cell FACS analysis were used to further characterize the binding of phage scFv. RESULTS: After transformation into E. coli TG1, 2.5 x 10(7) cfu/microg ampicillin-resistant clones grew. Sequences of those positive insert clones showed that the V(H) genes were derived from the V(H) III subgroup, while the V(L) genes belonged to the V(kappa) III subgroup. After four rounds of panning, the titer of eluted binding phage increased 135- to 158-fold and ELISA results showed that 20/95 clones can bind CEA and 47/95 clones can bind fixed tumor cells. Immunofluorescence and FACS analysis results showed that these phage scFv fragments could bind CEA-positive cells. CONCLUSIONS: We successfully constructed a human phage antibody library from lymph nodes of stomach cancer patients. Such kinds of library prove useful for generating tumor-antigen-specific human antibody fragments.

Amino Acid Sequence↗
PubMed1967

The Medical Library Association in retrospect, 1937-1967.

The medical librarian of 1967 lives in a period of changing concepts, dramatic new methods, everwidening scientific horizons. In looking toward the immediate past he may think of the medical librarian of thirty years ago as a complacent follower of accepted procedures, not as a pioneer in a brave new world. Yet the corps of trained medical librarians today and the resources of our collections and their management are dependent upon the efforts of those who were then pioneers in medical librarianship. Training, standards, recruitment, literature control, international relations, all had continuing attention at a time when financial assistance through government funds, support by administrators, concern by scientists was almost nonexistent. In these years the day of the devoted amateur passed; the trained medical librarian came into being and matured.This, the first Janet Doe Lecture, is named for one who illustrates the best in medical librarianship, serving with scholarly distinction. It is a brief survey pointing to some of the Association's significant achievements during the years of Miss Doe's greatest activity when she and her colleagues met their "Challenge of Change."

History, 20th Century↗
PubMed1998

The Medical Library Association in retrospect, 1937-1967.

The medical librarian of 1967 lives in a period of changing concepts, dramatic new methods, everwidening scientific horizons. In looking toward the immediate past he may think of the medical librarian of thirty years ago as a complacent follower of accepted procedures, not as a pioneer in a brave new world. Yet the corps of trained medical librarians today and the resources of our collections and their management are dependent upon the efforts of those who were then pioneers in medical librarianship. Training, standards, recruitment, literature control, international relations, all had continuing attention at a time when financial assistance through government funds, support by administrators, concern by scientists was almost nonexistent. In these years the day of the devoted amateur passed; the trained medical librarian came into being and matured. This, the first Janet Doe Lecture, is named for one who illustrates the best in medical librarianship, serving with scholarly distinction. It is a brief survey pointing to some of the Association's significant achievements during the years of Miss Doe's greatest activity when she and her colleagues met their "Challenge of Change".

History, 20th Century↗
PubMed1986

Standards for academic visual science libraries. Association of Visual Science Librarians.

These standards are a revised and updated version of the Guidelines and Standards for Visual Science Libraries Serving Optometric Institutions, a paper first issued by the Association of Visual Science Librarians (AVSL) in 1976. Their purpose is to provide minimum standards as an aid to accreditation bodies and to institutions initiating library service. In this revision, the Standards and Guidelines have been separated physically so that current information can be updated more frequently and easily. The Standards are a qualitative statement about the minimum levels of staff, collections, and services that should be expected from visual science libraries; the Guidelines contain suggested lists of journals and other quantitative data subject to frequent change. The Guidelines are available separately from the AVSL.

Architecture↗