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Determination of the absolute molecular weight averages and molecular weight distributions of alginates used as ice cream stabilizers by using multiangle laser light scattering measurements.

High-performance size exclusion chromatography with multiangle laser light scattering detection (HPSEC-MALLS) was used for characterizing complete molecular weight distributions for a range of commercial alginates used as ice cream stabilizers. For the samples investigated, molecular weight averages were found to vary between 115 000 and 321 700 g/mol and polydispersity indexes varied from 1. 53 to 3.25. These samples displayed a high content of low molecular weights. Thus, the weight percentage of material below 100 000 g/mol ranged between 6.9 and 54.4%.

Alginates↗

Comparison of methods for the recovery and detection of low levels of injured Salmonella in ice cream and milk powder.

This study compared the ability of four rapid methods and a standard cultural method to detect low levels of heat-injured cells of Salmonella typhimurium in ice cream and skimmed milk powder. The detection of Salmonella in samples contaminated with low levels (< 10 cfu 25 g-1) was significantly greater with the novel broth method than with the other methods (P </= 0.01). At contamination levels > 10 cfu 25 g-1, there was no significant difference between the methods except for the novel broth method and a dipstick-based immunoassay (P </= 0.05). The novel broth method, S.P.R.I.N.T. Salmonella, which incorporates a specifically formulated peptone and Oxyrase(R) combination followed by the timed release of selective agents into the recovery medium, was shown to improve the rate of detection of low numbers of injured cells of Salmonella after 24 h enrichment.

Animals↗