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At least 127 records · Page 7Linked to original sources
PubMed1991

Serum alpha 2-HS-glycoprotein levels in neonatal infections.

The serum concentration of alpha 2-HS-glycoprotein was determined in 15-infected neonates and 21 age-matched controls using the radial immunodiffusion method. The infected neonates had significantly lower serum alpha 2-HS-glycoprotein concentration than the controls (56.9 +/- 15.1 and 105.6 +/- 25.7, respectively; p less than 0.001) confirming that it also behaves as a negative acute phase reactant in neonates. No correlation was found between alpha 2-HS-glycoprotein and albumin concentrations. The serum glycoprotein concentration in the control neonates was significantly higher than that of healthy older children and reported values in adult controls. The often reported decrease in the opsonic function of the neonatal serum is therefore unlikely to be explained by decreased alpha 2-HS-glycoprotein levels. However, the relatively higher levels of the glycoprotein observed in this study in neonates may be related to the active bone mineralization taking place in the newborn period.

Bacterial Infections↗
PubMed1999

Levels of transferrin and alpha 2-HS glycoprotein in women with and without endometriosis.

The study objective was to test the hypothesis that elevated levels of transferrin and alpha 2-HS glycoprotein occur in the peritoneal environment of patients with endometriosis that may lead to the observed autoimmunity to these proteins. We set up a double sandwich enzyme-linked immunosorbent assay (ELISA) for measuring levels of transferrin and alpha 2-HS glycoprotein in the serum and peritoneal fluid samples from women with (n = 24-60) and without endometriosis (n = 35-49). Serum and peritoneal fluid levels of alpha 2-HS glycoprotein and peritoneal fluid levels of transferrin were significantly elevated in patients with endometriosis, in contrast to the controls. Serum levels of transferrin in patients, however, were significantly less in the patients than in controls. We conclude that transferrin and alpha 2-HS glycoprotein are present at high concentrations in the peritoneal fluids of patients with endometriosis. This may play a significant role in the autoimmune pathophysiology of endometriosis.

Ascitic Fluid↗
PubMed1992

[Diagnostic value of the determination of serum alpha2-HS-glycoprotein].

Opsonic glycoprotein, alpha 2-HS-glycoprotein concentration was studied in the serum of 753 patients with various hematological, malignant, immunological, metabolic, endocrine and liver diseases and 68 healthy controls. Decreased serum alpha 2-HS-glycoprotein levels were detected in patients with acute leukemias, chronic granulocyte and myelomonocyte leukemias, lymphomas, myelofibrosis, multiple myeloma, metastatizing solid tumors, systemic lupus erythematosus, rheumatoid arthritis, acute alcoholic hepatitis, fatty liver, chronic active hepatitis, liver cirrhosis, acute and chronic pancreatitis, and Crohn's disease. Elevated levels were measured in patients with B and NANB/C hepatitis. Further decreased levels were observed in some groups with secondary infections. Serum alpha 2-HS-glycoprotein levels are affected by many factors, influencing the synthesis and elimination of the protein. The detection of serum alpha 2-HS-glycoprotein concentration has no specific diagnostic value as a marker for tumors or other diseases, however, its determination can be useful for the assessment of a non-specific regulator of the host defence.

Blood Proteins↗
PubMed1987

Variations in alpha-2-HS glycoprotein level in neoplastic disease with and without involvement of bone.

Variations in the serum level of alpha-HS-glycoprotein may be observed in all pathological conditions which induce changes in bone turn-over, as well as in inflammatory and neoplastic diseases. This study includes 162 patients divided into four groups according to the TNM classification (tumour, lymph node metastasis). The first consisted of patients with neoplasms at TNM stages 1 and 2 with no bone metastases; the second of similar patients at TNM stages 3 and 4. The third group were patients with primary or secondary neoplasms of bone, and the fourth were patients with viral or bacterial diseases. The levels of alpha-2-HS-glycoprotein serum were determined for all the groups and these were compared with AAG (alpha-1-glycoprotein) and CEA (carcinoembryonic antigen). There were no significant differences in the levels of alpha-2-HS-glycoprotein for the first group as compared with normal controls, while in the other groups the differences were significant. The levels of alpha-2-HS-glycoprotein were diminished when the levels of CEA and AAG were both high, but increased when only one of these other parameters was high.

Adult↗
PubMed1982

[Alpha 2 HS-glycoprotein in rheumatoid arthritis. Its plasma concentration and possible biological role].

Alpha 2-HS glycoprotein is one of the plasma proteins found in high concentrations in bone tissue. In order to study the relationship between this glycoprotein plasma concentration and bone damage in rheumatoid arthritis, plasma concentrations of this substance were measured in the serums of 32 patients with rheumatoid arthritis before and after treatment. Our results demonstrated that the concentration of alpha 2-HS glycoprotein, although slightly higher than normal values, did not vary in rheumatoid arthritis. Treatment of the disease did not seem to modify alpha 2-HS glycoprotein plasma levels, whereas other biological and clinical parameters of inflammation improved. The probable biological role of alpha 2-HS glycoprotein in rheumatoid arthritis is debated.

Arthritis, Rheumatoid↗
PubMed1999

A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybrid SYT/SSX1 gene.

Recent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX (SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RT-PCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3' end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3' side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene.

Aged↗
PubMed1983

Failure of the Hs-PT index to distinguish organic from functional patients.

Investigated the effectiveness of the MMPI Hs-PT index to separate patients with a brain disorder from those with functional disturbance. A sample of 303 male patients who were referred for neuropsychological evaluation because of known or suspected brain damage was used in the study. Of these, 187 were diagnosed as organic and 116 as functional. The Hs-PT index failed to separate these groups beyond a chance level. Hit rates were 52.4% for the organic patients and 36.2% for the functional patients using the cut-off established in previous research. No discriminations were possible at any of the Hs-PT ranges. Failure of the Hs-PT index to hold up in this sample of patients is probably due to some very different patient characteristics from those prevalent in earlier research.

Adolescent↗
PubMed1996

The role and amplification of the HS Alu subfamily founder gene.

A recently identified Alu element (Leeflang et al. J. Mol. Evol. 1993, 37:559-565), referred to as the "putative founder of the HS (PV) subfamily," was found to be present at orthologous loci in the human, chimpanzee, gorilla, and gibbon lineages. The evolution of this Alu suggested that it is a source gene in the evolution of Alu family repeats for one of the most recent subfamilies, HS. We have determined that this putative founder of the HS subfamily was not present at the orthologous loci in older primates, including old world and new world monkeys. Thus, this particular Alu locus has only been responsible for the establishment of a very small subfamily of Alu sequences. We have further demonstrated that this putative founder Alu was not responsible for the de novo Alu insertion into the neurofibromatosis-1 gene of an individual causing neurofibromatosis. Our data demonstrate that although the putative founder of the HS subfamily found by Leeflang et al. (1993) probably gave rise to one of the most recent subfamilies of Alu sequences, it has not been very active in retroposition.

Animals↗
PubMed2003

Headspace solid-phase microextraction (HS-SPME): a microscale sampling technique for determination of monoterpene hydrocarbons in coniferous needles by gas chromatography/mass spectrometry (GC/MS).

A headspace solid-phase microextraction (HS-SPME) technique has been applied for the microscale sampling (single needle for Picea omorica, two needles of Picea abies) of volatile monoterpene hydrocarbons (MTHs) from conifer needles. A simple device consisting of a closed headspace vial equipped with an integral cutting device was used for the collection, homogenisation, and HS-SPME sampling. The highly sensitive gas chromatographic/mass spectrometric (GC/MS) analyses (LODs in tenths of ng g(-1) FW were obtained for 3xS/N criteria) of individual needles confirmed the space distribution of MTHs in different parts of a single sprout (base, centre, apex) and among the left, central and right sprout of a whorl. The highest concentrations of MTHs were found in the apical (leading) sprout (S) of a whorl. The serious increase of MTHs content near the base of the left (SNL) sprout and nearly zero concentrations in the central parts of both the side sprouts of P. abies corresponded to the morphology of the whorl. On the other hand, similar concentrations of MTHs were found in the apex of the side (SNL and SNR) sprouts. The distributions of MTHs obtained after HS-SPME and solvent extraction followed the similar trends for both species. HS-SPME is more suitable for the evaluation of distribution of volatiles in microscale experiments due to the more precise separation of needles into correct categories and elimination of "averaging" effects of the larger quantities of needles needed for the solvent extraction. The precision was improved by one order of magnitude due to the reduction of uncontrolled losses of volatile species during the sample treatment.

Gas Chromatography-Mass Spectrometry↗
PubMed1991

Microbial polysaccharide, HS-142-1, competitively and selectively inhibits ANP binding to its guanylyl cyclase-containing receptor.

During the search for ANP receptor ligands of microbial origin, we isolated a novel polysaccharide, HS-142-1, from culture broth of Aureobasidium sp. HS-142-1 inhibited [125I]-rANP binding to ANP receptor in rabbit kidney cortex membranes with an IC50 of 0.3 mu g/ml, but gave no effects on specific binding of [125I]-Endothelin nor [125I]-Angiotensin II to their respective receptors in bovine lung membranes. HS-142-1 competitively and selectively inhibited ANP binding to its guanylyl cyclase-containing receptor purified from solubilized bovine adrenocortical membranes and blocked cGMP production elicited by ANP. HS-142-1 is the first non-peptide antagonist selective for ANP functional receptor and will be a powerful tool to elucidate the physiological functions of ANP.

Adrenal Cortex↗
PubMed1992

Stable expression of natriuretic peptide receptors: effects of HS-142-1, a non-peptide ANP antagonist.

We established clonal cell lines stably expressing each of two subtypes of membrane bound guanylate cyclases (GC-A and GC-B), which are known as natriuretic peptide receptors. Using these cell lines, we showed that GC-A is an ANP/BNP receptor, whereas GC-B is a specific receptor for CNP. Effects of HS-142-1, a novel non-peptide ANP antagonist, on GC-A and GC-B were examined by using these cells. In cells expressing either GC-A or GC-B, HS-142-1 inhibited cGMP production elicited by ANP or CNP with IC50 values of 1.8 micrograms/ml and 1.5 micrograms/ml, respectively, and also competitively blocked specific binding of the natriuretic peptides with IC50 values of 2.2 micrograms/ml and 3.3 micrograms/ml, respectively. These results indicate that HS-142-1 is a potent antagonist of CNP as well as ANP. We also showed that CNP suppressed the growth of cells expressing GC-B by 22% and that HS-142-1 blocked the antiproliferative action of CNP.

Animals↗
PubMed1993

HS-142-1, a novel antagonist for natriuretic peptides, has no effect on the third member of membrane bound guanylate cyclases (GC-C) in T84 cells.

HS-142-1, a novel non-peptide antagonist for natriuretic peptides, exerts antagonistic actions almost equally on two similar guanylate cyclase-linked natriuretic peptide receptors (GC-A and GC-B), but has little or no effect on the binding of natriuretic peptides to a membrane protein, the so-called "clearance receptor", which binds all natriuretic peptides. The third mammalian form of membrane bound guanylate cyclases (GC-C) was identified not as a natriuretic peptide receptor, but as a receptor for heat-stable enterotoxins (STa). In this study, we examined effects of HS-142-1 on GC-C (STaR) in T84 cells and showed that HS-142-1 exerts neither agonistic nor antagonistic activity for GC-C, indicating that HS-142-1 is not a common antagonist for a family of membrane bound guanylate cyclase receptors, but a specific antagonist for the guanylate cyclase-linked natriuretic peptide receptors.

Atrial Natriuretic Factor↗
PubMed1992

HS-142-1, a novel non-peptide ANP antagonist, blocks the cyclic GMP production elicited by natriuretic peptides in PC12 and NG108-15 cells.

HS-142-1 is a novel non-peptide antagonist for atrial natriuretic peptide (ANP) receptor. The effect of HS-142-1 on the cyclic GMP production elicited by natriuretic peptides in neuronal cell lines, PC12 and NG108-15 was examined. Natriuretic peptides such as ANP, brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) enhanced cyclic GMP production in a dose-dependent manner. HS-142-1 inhibited cyclic GMP accumulation elicited by natriuretic peptides in a dose-dependent fashion in both cells. The results suggest that HS-142-1 will be an important tool for identification and understanding of the mechanisms by which natriuretic peptides act in nervous systems.

Animals↗
PubMed2004

Serum iron, ferritin, transferrin, total iron binding capacity, hs-CRP, LDL cholesterol and magnesium in children; new reference intervals using the Dade Dimension Clinical Chemistry System.

BACKGROUND: We studied serum/Plasma iron, ferritin, transferrin concentrations, and total iron binding capacity (TIBC) in addition to highly sensitive C-reactive protein (hs-CRP), low-density lipoprotein (LDL) cholesterol and magnesium concentrations in a population of 800 in- and outpatients and defined new 95% reference intervals for pediatrics using the Dade Behring RxL Dimension Clinical Chemistry System. METHODS: Plasma/serum concentrations of the above analytes were determined on patient samples accrued from January to June 2003 and the data were analyzed employing a computer adapted Hoffmann approach. RESULTS AND CONCLUSIONS: New pediatric reference intervals on the Dade RxL Dimension were obtained for serum iron, ferritin, transferrin, TIBC, hs-CRP, LDL-cholesterol and magnesium. This work represents the first hs-CRP data for children <3 years old. The values for all the analytes were established on new data (n=800). These values are new for hs-CRP and LDL cholesterol, while for iron, ferritin and magnesium these intervals are more reliable than those previously published by our group which had used regression equations on samples measured by the old (Vitros/Immuno1) methods for which intervals were available.

Adolescent↗
PubMed2005

Brief review and critical examination of the use of hs-CRP for cardiac risk assessment with the conclusion that it is premature to use this test.

BACKGROUND: Inflammation appears to be an important ingredient in the process of atherosclerosis that leads to coronary artery disease. Some have designed an algorithm for assessing risk of coronary artery disease using the inflammatory marker hs-CRP in conjunction with lipoprotein lipid risk factors. AIM: I contend that because of its poor discrimination for coronary risk in clinical trials, until its utility is better proven, hs-CRP should not be recommended for defining risk. REVIEW: Published articles and reassessment of papers previously published. CONCLUSIONS: After adjustment for conventional risk factors, hs-CRP discriminates poorly between persons with coronary disease and those without over a range of from about 0.6 to 7 mg/L which includes most apparently well people. Nor does the evidence indicate that hs-CRP adds significant predictive value to the clinical traits that define metabolic syndrome.

C-Reactive Protein↗
PubMed1986

Component C of the methylcoenzyme M methylreductase system contains bound 7-mercaptoheptanoylthreonine phosphate (HS-HTP).

The structure of component B of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum was recently found to be 7-mercaptoheptanoylthreonine phosphate (HS-HTP). The work described here demonstrates that this compound is found in two forms: enzyme-free and enzyme-bound. HS-HTP was found to be bound to component C of the methylcoenzyme M methylreductase system. The cofactor extracted from the protein by heat denaturation was found to comigrate with the mixed disulfide of HS-HTP and 2-mercaptoethanol by high-performance liquid chromatography, suggesting HS-HTP is not modified in the bound state.

Chromatography, High Pressure Liquid↗
PubMed2002

Nucleophilic aromatic substitution reactions of chloroazines with bisulfide (HS-) and polysulfides (Sn2-).

Reactions of bisulfide and polysulfides with chloroazines (important constituents of agrochemicals and textile dyes) were examined in aqueous solution at 25 degrees C. For atrazine, rates are first-order in polysulfide concentration, and polysulfide dianions are the principal reactive nucleophiles; no measurable reaction occurs with HS-. Second-order rate constants for reactions of an array of chloroazines with polysulfides are several orders of magnitude greater than for reactions with HS-. Transformation products indicate the substitution of halogen(s) by sulfur. Ring aza nitrogens substantially enhance reactivity through a combination of inductive and mesomeric effects, and electron-withdrawing or electron-donating substituents markedly enhance or diminish reactivity, respectively. The overall second-order nature of the reaction, the products observed, and reactivity trends are all consistent with a nucleophilic aromatic substitution (S(N)Ar) mechanism. Rate constants for reactions with HS- and Sn2- (n = 2-5) correlate only weakly with lowest unoccupied molecular orbital energies, suggesting that the electrophilicity of a chloroazine is not the sole determinant of its reactivity. When second-order rate constants are extrapolated to HS- and Sn2- concentrations reported in salt marsh pore waters, half-lives of minutes to years are obtained. Polysulfides in particular could play an important role in effecting abiotic transformations of chloroazines in hypoxic marine waters.

Azo Compounds↗
PubMed2006

Collision-induced dissociation of HS-(HCN): unsymmetrical hydrogen bonding in a proton-bound dimer anion.

The energy-resolved competitive collision-induced dissociation of the proton-bound complex [HS.H.CN](-) is studied in a guided ion beam tandem mass spectrometer. H(2)S and HCN have nearly identical gas-phase acidities, and therefore, the HS(-) + HCN and the CN(-) + H(2)S product channels exhibit nearly the same threshold energies, as expected. However, the HS(-) + HCN channel has a cross section up to a factor of 50 larger than CN(-) + H(2)S at higher energies. The cross sections are modeled using RRKM theory and phase space theory. The complex dissociates to HS(-)+ HCN via a loose transition state, and it dissociates to CN(-) + H(2)S via a tight transition state. Theoretical calculations show that the proton-transfer potential energy surface has a single minimum and that the hydrogen bonding in the complex is strongly unsymmetrical, with an ion-molecule complex of the form HS(-)..HCN rather than CN(-)..H(2)S or an intermediate structure. The requirement for proton transfer before dissociation and curvature along the reaction path impedes the CN(-) + H(2)S product channel.

Journal Article↗