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Pleurotus mushrooms. Part I A. Morphology, life cycle, taxonomy, breeding, and cultivation.

Pleurotus species represent a well-defined group of Basidiomyceteous fungi of the order Agaricales and family Tricholomataceae. They are characterized by the production of fruit bodies with an eccentric stalk and a wide cap shaped like an oyster shell, with the widest portion of the cap being away from the stalk. They grow over a wide range of temperatures and are able to colonize a wide spectrum of unfermented, natural, lignino-cellulosic wastes. Because of their fast mycelial growth rate, they colonize the substrates rapidly; the yield of fruit bodies is also high. The bifactorial inheritance, observed in many of the species, suggests the likelihood of a high degree of genetic variability, and, hence, considerable breeding potential. Pleurotus species can be grown on a commercial scale, without the need for composting and artificial conditioning of the ambient temperature. Aspects of basic and applied research--such as morphology, life cycle, taxonomy, breeding, and cultivation--leading to development of a "Pleurotus Technology" are dealt with here.

Basidiomycota↗

Development of mass trapping technique for control of brinjal shoot and fruit borer, Leucinodes orbonalis (Lepidoptera: Pyralidae).

Locally-produced clear plastic water traps (12 cm x 14 cm base and 21 cm height) were optimized for use in large-scale mass trapping trials for control of brinjal fruit and shoot borer, Leucinodes orbonalis Guenée. Changing the shape (square and triangular) and number (two and four) of entry holes in the water trap had no significant effect on trap catch. Significantly more male moths were caught in traps treated with water containing powdered detergent than liquid detergent, light gear oil or insecticide. All water traps tested caught significantly higher numbers of moths than sticky delta traps with open sides under farmers' field conditions. Trap catches per 100 m2 were found to increase with increasing number of traps from 3 to 6 but the difference in catch between 4 and 6 traps per 100 m2 was not significant. Two small-scale replicated integrated pest management (IPM) trials were conducted consisting of the optimized water trap placed out with 10 m spacing (4 per 100 m2) and infested shoots pruned and destroyed. The first season trial had two treatments, IPM and farmers' practice in which farmers applied insecticide every two days in the peak harvest period. Overall, the percentage of healthy fruit and yields in both treatments were comparable at 53.8 and 49.6% and 20 and 19.4 tonnes per ha in the IPM and farmers' practice plots respectively. However, the initial infestations in the IPM plots (68%) were significantly higher than in farmers' practice plots (16%) due to the proximity of the nurseries used for the IPM plots to stacks of brinjal crop residues from the previous season that acted as a source of infestation. The second season's trials contained a third treatment in which IPM and farmers' practice were combined. The percent total healthy fruits harvested were 46.1, 58.6 and 69.1% respectively for the farmers' practice, farmers' practice plus IPM and IPM alone. Averaged total fruit yields were approximately 12 tonnes per ha for the farmers' practice plots and 30 tonnes per ha for each of the IPM-treated plots. The IPM plot had significantly fewer infested fruit than the IPM plus farmers practice plots and this was attributed to the activity of the larval parasitoid Trathala flavo-orbitalis (Cameron) that was suppressed in trial plots treated with insecticides.

Animals↗

Stimulus categorization by brain-damaged patients.

Patients with localized insult to the right hemisphere, or to either the anterior or the posterior portion of the left hemisphere, as well as neurologically intact controls, evaluated stimuli on a seven-point rating scale for their degree of category membership. The stimuli were taken from one of two continua, one composed of fruit and vegetable items, and the other of items differing in hue and shape. Different subsets of stimuli provided different contexts for the judgments of category membership. The two left-hemisphere groups showed anomalies in categorizing the fruit and vegetable items but not the perceptual items, while the reverse was true for the right-hemisphere patients. Moreover, both left-hemisphere groups demonstrated context effects in their judgments of the representativeness of the fruit and vegetable items, but differed in the way in which they responded to changing contexts. Left posterior patients demonstrated weak category boundaries and even reclassified items. In contrast, patients with left anterior damage showed highly categorical responses and less differentiation of items within a category. All groups showed striking context effects in judgments of perceptual items in terms of changes in representativeness ratings and the location of a category boundary. Alternative interpretations of the results are offered.

Brain Damage, Chronic↗

Physical Aspects of Fruit Growth : THEORETICAL ANALYSIS OF DISTRIBUTION OF SURFACE GROWTH FORCES IN FRUIT IN RELATION TO CRACKING AND SPLITTING.

The theory of shells has been applied to some aspects of the physics of fruit growth. Four form and structural attributes are identified which may intensify mechanical stress in the skin of a growing fruit and alter the distribution of that stress. One is a radius-related factor introduced by deviation of shape from that of a sphere and the other three are related to attachment of a fruit to a plant and to provision of a vascular system: core diameter, core tensile strength, and structure of the core/skin interface. The last factor also applies in principle to a hole which may be introduced for example at the style canal. These factors either alone or in combination can cause stresses far in excess of those predicted for a spherical shell of similar volume and wall thickness. They are considered in relation to their effect on fruit morphogenesis and the occurrence of disorders such as rainfall-induced splitting and cracking.

Journal Article↗

D-galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress.

D-galactose causes aging acceleration in different animal models but the mechanism is unclear. In the present study, we investigated the effects of D-galactose on lifespan and oxidative stress biomarkers in the fruit fly (Drosophila melanogaster) and housefly (Musca domestica). D-galactose was added to drinking water (20 mg/ml) for housefly and to culture medium (6.5%) for fruit fly from 24 h after emergence. Oxidative stress was estimated by measuring the activity of Cu-Zn-superoxide dismutase (SOD) and the levels of lipid peroxidation products, namely malondialdehyde (MDA) and lipofuscin in housefly brain (male) and in fruit fly (male and female). D-galactose caused a significant decrease in mean lifespan (by 12.6% of male and 15.9% of female) and maximum lifespan (by 12.9% of male and 17.1% of female) in fruit fly, and also a significant decrease in mean lifespan (by 27.1% of male, 19.8% of female) and maximum lifespan (by 27.1% of male, 21.9% of female) in housefly. MDA and lipofuscin increased with age in fruit fly and in housefly brains while change of the SOD activity showed a biphasic shape with age. D-galactose caused a significant increase in MDA and lipofuscin and decrease in SOD activity in the age-matched fruit flies and houseflies. These data indicate that D-galactose shortens the lifespan of the two different fly species and that the life shortening effect is associated with an increase in oxidative stress.

Animals↗

Air volume measurement of 'Braeburn' apple fruit.

The radial disposition of air in the flesh of fruit of Malus domestica Borkh., cv 'Braeburn' was investigated using a gravimetric technique based on Archimedes' principle. Intercellular air volume was measured by weighing a small tissue sample under water before and after vacuum infiltration to remove the air. In a separate procedure, the volume of the same sample was measured by recording the buoyant upthrust experienced by it when fully immersed in water. The method underestimates tissue air volume due to a slight invasion of the intercellular air spaces around the edges of the sample when it is immersed in water. To correct for this error, an adjustment factor was made based upon an analysis of a series of measurements of air volume in samples of different dimensions. In 'Braeburn' there is a gradient of declining air content from just beneath the skin to the centre of the fruit with a sharp discontinuity at the core line. Cell shape and cell packing were observed in the surface layers of freshly excised and stained flesh samples using a dissecting microscope coupled to a video camera and a PC running proprietary software. Tissue organization changed with distance below the skin. It is speculated that reduced internal gas movement, due to the tightly packed tissue of 'Braeburn' and to the potential diffusion barrier at the core line between the cortex and the pith, may increase susceptibility of the flesh to disorders associated with tissue browning and breakdown.

Air↗

Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess omega-alicyclic fatty acids, and emended description of the genus Alicyclobacillus.

A thermo-acidophilic endospore-forming bacterium was isolated from a mixed fruit juice. The organism, strain 3A(T), was rod-shaped, grew aerobically at 30-60 degrees C (optimum 45-50 degrees C), pH 3.0-6.0 (optimum pH 4.0-4.5) and produced acid from various sugars. It contained menaquinone-7 as the major isoprenoid quinone. The G+C content of the DNA was 53.1 mol%. The predominant cellular fatty acids of the strain were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0) and anteiso-C(17 : 0), but omega-alicyclic fatty acids, which are characteristic of the genus Alicyclobacillus, were not found in the strain. Phylogenetic analyses based on both 16S rRNA and gyrB (DNA gyrase B subunit gene) gene sequences showed that strain 3A(T) falls into the Alicyclobacillus cluster, validated by significant bootstrap values. However, strain 3A(T) did not show a close relationship to the other species of the cluster. The level of 16S rDNA similarity between strain 3A(T) and other strains of the cluster was between 92.5 and 95.5 %. The level of gyrB sequence similarity between strain 3A(T) and other strains of the cluster was between 68.5 and 74.4 %. DNA-DNA hybridization values between strain 3A(T) and phylogenetically related strains of the genera Alicyclobacillus, Bacillus and Sulfobacillus were under 13 %, indicating that strain 3A(T) represents a distinct species. On the basis of these results, strain 3A(T) should be classified as a novel Alicyclobacillus species. The name Alicyclobacillus pomorum is proposed for this organism. The type strain of Alicyclobacillus pomorum is strain 3A(T) (=DSM 14955(T)=IAM 14988(T)).

Base Composition↗

Spatial control of cell differentiation in Myxococcus xanthus.

Myxococcus xanthus develops species-specific multicellular fruiting bodies. Starting from a uniform mat of cells, some cells enter into nascent fruiting body aggregates, whereas other cells remain outside. The cells within the fruiting body differentiate from rods into spherical, heat-resistant spores, whereas the cells outside the aggregates, called peripheral cells, remain rod-shaped. Early developmentally regulated genes are expressed in peripheral cells as well as by cells in the fruiting bodies. By contrast, late developmental genes are only expressed by cells within the nascent fruiting bodies. The data show that peripheral cells begin to develop, but are unable to express genes that are switched on later than about 6 h after the start of development. All of the genes whose expression is limited to the fruiting body are dependent on C-signaling either directly or indirectly, whereas the genes that are equally expressed in peripheral rods and in fruiting body cells are not. One of the C-signal-dependent and spatially patterned operons is called dev, and the dev operon has been implicated in the process of sporulation. It is proposed that expression of certain genes, including those of the dev operon, is limited to the nascent fruiting body because fruiting body cells engage in a high level of C-signaling. Peripheral cells do less C-signaling than fruiting body cells, because they have a different spatial arrangement and are at lower density. As a consequence, peripheral cells fail to express the late genes necessary for spore differentiation.

Artificial Gene Fusion↗

An evolutionary explanation of the aggregation model of species coexistence.

In ecology, the 'aggregation model of coexistence' provides a powerful concept to explain the unexpectedly high species richness of insects on ephemeral resources like dung pats, fruits, etc. It suggests that females aggregate their eggs across resource patches, which leads to an increased intraspecific competition within occupied patches and a relatively large number of patches that remain unoccupied. This provides competitor-free patches for heterospecifics, facilitating species coexistence. At first glance, deliberately causing competition among the females' own offspring and leaving resources to heterospecific competitors seems altruistic and incompatible with individual fitness maximization, raising the question of how natural selection operates in favour of egg aggregation on ephemeral resource patches. Allee effects that lead to fitness maxima at intermediate egg densities have been suggested, but not yet detected. Using drosophilid flies on decaying fruits as a study system, we demonstrate a hump-shaped relationship between egg density and individual survival probability, with maximum survivorship at intermediate densities. This pattern clearly selects for egg aggregation and resolves the possible conflict between the ecological concept of species coexistence on ephemeral resources and evolutionary theory.

Animals↗

Wing shape heritability and morphological divergence of the sibling species Drosophila mercatorum and Drosophila paranaensis.

The fruit-flies Drosophila paranaensis and Drosophila mercatorum pararepleta are sibling species belonging to the repleta group. Females of these two species are normally considered to be morphologically indistinguishable while males only differ consistently in the morphology of their genitalia. These species are sympatric throughout a large area of their geographic distribution. In this study, we investigated the degree of morphological divergence between D. paranaensis and D. mercatorum pararepleta based on morphometric analysis of their wings. The ellipse method was used to describe the placement of the longitudinal and transversal wing veins as well as the size of the wing and the shape of its outline. The heritability under laboratory and field conditions was also estimated from the parameters generated. Multivariate analysis showed that wing morphology possessed sufficient differences to discriminate between the two species with a successful classification rate of 95-98% for females and 82-87% for males. The results of the autoclassification were confirmed by a cross-validation test for females (92-96%). Most measurements possessed significant natural heritability (a mean of 0.48 for D. mercatorum and 0.88 for D. paranaensis), indicating that the variation observed was related to differences in genes acting additively. The principal difference between the two species was in the placement of the posterior transverse wing vein. However, the pattern of morphological variation in the wings of both species was similar, possibly because of shared restrictions in wing development pathways.

Animals↗

Modeling of the sorption and diffusion processes of volatile organic air pollutants in grape fruits.

A mathematical model for the description of the sorption and diffusion processes of gaseous toluene and p-xylene in fruits of grape has been proposed. This model is based on the Fick's II low regarding a particle with a spherical shape (such as the berry of grapevine) and describes changes of air pollutant concentrations in different layers of the fruit, that is, the wax, peel, and pulp, during an exposure to contaminated air. The mass transfer coefficient and diffusion coefficients in the respective layers can be estimated using the experimental values. The theoretical data and the results from the exposure under steady-state laboratory conditions were compared and showed a good applicability of the proposed model for the prediction of volatile air pollutant partitioning in grape berries.

Air Pollutants↗

Seasonal variations in black-faced black spider monkey (Ateles chamek) habitat use and ranging behavior in a southern Amazonian tropical forest.

Data are presented regarding the habitat use and ranging behavior of a spider monkey (Ateles chamek) community at Lago Caiman in northeastern Bolivia. Habitat use was driven primarily by fruit availability and distribution across the community home range. Strong seasonal variations occurred in fruit availability within all five of the floristically and phenologically distinct habitat types identified within the study site, and the spider monkeys dramatically shifted their ranging according to which habitat was richest in fleshy fruits. This use of local habitat diversity resulted in an unusually elongated shape for the home range that was otherwise typical of previous Ateles studies in terms of size. Ranging behavior was clumped and community core areas shifted seasonally across the focal community home range. Individual core areas were not relevant to the study due to dramatic community-wide shifts in ranging patterns. Day journey lengths were highly variable (460-5,690 m) and the distribution and abundance of fleshy fruit resources explained 81% of the monthly variations in mean day journey length. Keystone habitats for forest frugivores are identified and results are discussed with reference to previous studies on this genus, and the importance of considering keystone habitats and local habitat diversity within the management of forestry concessions in the region. Results are also discussed with reference to the behavioral ecology of the genus Ateles.

Animal Migration↗

Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells.

To characterize the function of plant profilins in vivo, we expressed two pollen specific Zea mays (maize) profilin isoforms in profilin-minus Dictyostelium discoideum mutants. In maize, profilins exist as a multigene family containing 4 or more members which are highly similar to each other but substantially less similar to profilins from animals and lower eukaryotes. Previously we have shown that D. discoideum profilin-minus cells have an aberrant phenotype due to defects in cell shape, cytokinesis, and development. These defects could be rescued by introducing the pollen-specific profilins 1 or 2 from maize using a newly constructed expression vector. Expression of the heterologous profilins in Dictyostelium clones was assayed by affinity purification of the pollen profilins with poly-proline agarose and by immunoblotting with a polyclonal antiserum raised against maize pollen profilin. In contrast to the profilin-minus mutants, Dictyostelium cells expressing plant profilins showed normal cell shape, contained less F-actin, and were able to form fruiting bodies. These data provide genetic evidence that maize pollen profilins, even though they are specific for a distinct developmental stage, share functional properties with profilin from a lower eukaryote and apparently act as G-actin-sequestering proteins in this system.

Animals↗

The index line as a new aid in clinical and forensic toxicology.

On behalf of the German Federal Ministry for Research and Technology, we investigated the use of electronic data processing for clinical toxicological purposes. Initially, sufficient funds were available for a comprehensive approach to the problem and programs covering the following areas were established: 1. A casuistic data program; 2. an identification program for wild fruits; 3. a program for the identification of tablets by their shapes. Due to a subsequent lack of funds, it was necessary to develop a partial solution: this solution is what we call the Index Line. The Index Line--limited data on poisonings--should enable the user to receive information by telex from the German Institute for Medical Documentation and Information, i.e., information on "who has what, where". As a first step the continuous Index Line registration of all cases of poisoning recorded at the poison control centers in Munich, Freiburg, Hamburg, and Nürnberg as well as at the State Institute for Food, Pharmaceutical and Forensic Chemistry in Berlin was founded in 1975. To participate in the program, complete instructions are necessary.

Computers↗

Fruit, vegetables, and the prevention of cancer: research challenges.

OBJECTIVE: A great deal of epidemiologic evidence has indicated that fruits and vegetables are protective against numerous forms of cancer. However, there are many gaps in our knowledge. METHODS: In this pilot study we reviewed more than 200 cohort and case-control studies to determine the shape of the dose-response relationship (i.e., how the risk reduction per extra serving of fruits and vegetables changes with the actual intake of these foods). We found major barriers to investigating this. As part of this pilot study we also investigated whether specific fruits and vegetables are responsible for the anticancer action of these foods or whether a wide variety is required for optimal protection. If the former is correct, then fruits and vegetables may contain one or a small number of "magic bullets"; if the latter is correct, then a "teamwork" concept may be valid. RESULTS: Different findings suggested that the teamwork concept is much more likely. Many studies, especially older ones, have ignored potential confounding variables such as energy intake, alcohol consumption, physical activity, body mass index, smoking, and socioeconomic status (although many recent studies have adjusted for education). Other potential confounders that have generally been ignored are consumption of whole grain cereals and the use of vitamin and mineral supplements. CONCLUSIONS: The inverse association between intake of fruits and vegetables and the risk of cancer of the colon, breast, and stomach has generally been much stronger in case-control than in cohort studies. We have no clear explanation for this.

Case-Control Studies↗

The creative aspect of dynamic psychotherapy: parallels between the construction of experienced reality in the literary and the psychotherapeutic process.

Aesthetic experience and psychotherapeutic practice are compared. Beginning with the assumption that memories, imaginings and feelings are aesthetic categories, it is shown that aesthetic experience is elementary in the shaping and structuring of the experiences of human beings, both in psychotherapeutic communication and in the process of production and reception in literature. The aesthetic shaping of events into integrated experiences in the course of the therapeutic encounter might have a healing effect in its own right. To this extent, the therapeutic process should be regarded both as a joint process of creation and as a process of interpretation. As in writing and reading a literary work of art, the therapeutic process consists of special functions of productivity/poiesis, receptivity/aesthesis, and communicative interaction/catharsis. The psychic mechanism of projective identification may help us understand this emotional and cognitive interchange psychodynamically. Further graphic associations and visual imaginings that arise in the patient and in the therapist are considered, like literary works of art, to function as "transitional objects." These creations of patient and therapist, artist and recipient serve to consolidate formerly nonintegrated and therefore pathogenic experiences. A fruitful psychodynamic work is always situated in the tense relationship between the aesthetic shaping of emotions and the scientific interpretation of inner and outer facts.

Adult↗

Overexpression of a gene encoding a cytochrome P450, CYP78A9, induces large and seedless fruit in arabidopsis.

An activation tagging screen in which the cauliflower mosaic virus 35S enhancer was inserted randomly into an Arabidopsis genome homozygous for the floral homeotic mutation apetala2-1 (ap2-1) resulted in a line (28-5) with extraordinarily wide, heart-shaped ovaries. The ovary of the 28-5 ap2-1 mutant shows an oval shape because of increased numbers of enlarged cells. When the ap2-1 mutation is crossed out of the genetic background, more elongated rather than wider fruits are obtained. Normally, Arabidopsis fruits will develop to a normal size only when the ovules are present and fertilized. In the 28-5 single mutant, the siliques keep growing despite failure of fertilization and can reach nearly normal size. When wild-type pollen was used to pollinate the mutant pistil, the pollinated 28-5 silique became >10% longer and 40% wider than a wild-type silique, although producing very few seeds. The enhancer insertion in line 28-5 acts by hyperactivating a cytochrome P450 gene, CYP78A9. The pistil of 28-5 ap2-1 mutant flowers shows a structure similar to that of Capsella bursa-pastoris, a distant mustard relative of Arabidopsis, suggesting that the processes regulated by the CYP78A9-encoded protein may be involved in evolutionary control of carpel shape.

Arabidopsis↗