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PubMed1994

Levels of insulin-like growth factor-binding protein-2 and insulin-like growth factor-II in maternal serum, amniotic fluid and extraembryonic coelomic fluid at 9-20 weeks of pregnancy.

Insulin-like growth factor-II (IGF-II) and IGF-binding protein-2 (IGFBP-2) were measured in amniotic fluid, extraembryonic fluid and maternal serum from 20 women with apparently normal first trimester pregnancies prior to termination. A further 111 specimens of amniotic fluid were collected from women at 10-20 weeks of pregnancy. Levels of IGFBP-2 were similar in coelomic fluid and maternal serum. Levels in amniotic fluid were lower than those in serum and coelomic fluid (Mann-Whitney test; P = 0.0002 and P < 0.0001 respectively). The levels of IGF-II were much higher in maternal serum than in coelomic fluid, and higher in the latter than in amniotic fluid (Mann-Whitney test; P < 0.0001 for both situations). The levels of IGFBP-2 were relatively low at 10-11 weeks (medians 19.8 and 61.1 micrograms/l) but thereafter increased to 20 weeks (median 1400 micrograms/l). The levels of IGF-II showed a similar pattern. The findings suggest that the role of IGF-II and IGFBP-2 in the regulation of growth or differentiation of the fetus or of its surrounding membranes may change with advancing pregnancy.

Adult↗

PubMed2000

Biomathematical modelling of physiological fluids using a Casson fluid with emphasis to peristalsis.

In this paper, the peristaltic flow of rheologically complex physiological fluids when modelled by a non-Newtonian Casson fluid in a two-dimensional channel is considered. Of interest is the difference between peristaltic transport of Newtonian and non-Newtonian fluids. A perturbation series method of solution of the stream function in amplitude ratio is sought. It is found that Newtonian fluid is an important sub-class of non-Newtonian fluids that may adequately represent some physiological phenomena. It is shown that for a Casson fluid, when certain simplifications and approximations are made in the most generalised form of constitutive equation, the fluid may be adequately represented as an improvement of a Newtonian fluid.

Body Fluids↗

PubMedDate not supplied

Role of subvoxel free fluid on diffusion parameters in brain tissue with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy and its correlation with physical disability: histogram analysis of standard and fluid-attenuated MR diffusion.

BACKGROUND AND PURPOSE: Subcortical signal intensity abnormalities and lacunar infarcts are the radiologic hallmark of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. MR diffusion studies reveal abnormalities in lesions and also within normal appearing white matter. To further characterize the underlying pathologic abnormality, we evaluated the role of subvoxel free fluid in brain with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy on diffusion parameters and physical disability and analyzed the interrelation between diffusion variables and nonlacunar T2 lesion load. METHODS: Mean diffusivity maps from fluid-attenuated and standard diffusion images of 13 patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy and seven age-matched control participants were compared by means of histogram analysis for three tissue compartments (whole brain parenchyma, normal appearing brain tissue, and nonlacunar lesions) by using a semiautomated region growing algorithm to define whole brain parenchyma and lesions on fluid-attenuated images. RESULTS: In both patients and control participants, the average mean diffusivity of whole brain parenchyma was lower on fluid-attenuated than on standard images (P <.001). Average mean diffusivity and peak location for all compartments were significantly elevated in patients (P <.001) and higher for lesions than for normal appearing brain tissue on both types of images (P <.001). The difference between standard and fluid-attenuated average mean diffusivity of normal appearing brain tissue, reflecting the subvoxel free fluid content, was elevated in patients (P <.05) and correlated closely with the Rankin score (Spearman's rank correlation coefficient = 0.889, P <.001). Average mean diffusivity of whole brain parenchyma and normal appearing brain tissue correlated strongly with the nonlacunar T2 lesion load (Pearson's correlation coefficient = 0.743-0.928, P <.005). CONCLUSION: This study shows that standard diffusion measurements are contaminated by free fluid partial volume effects for patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy and for control participants. It also provides evidence of a clinical significance of increased subvoxel free fluid in normal appearing brain with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, which may be more important than either global atrophy, increased diffusivity or, T2 lesion load.

Adult↗

PubMed1992

[Fluid-fluid levels in bone and soft tissue tumors demonstrated by MR imaging].

Fluid-fluid levels in bone tumors have been described in aneurysmal bone cysts and other cystic tumors of bones and soft tissue tumors. We experienced three bone tumors (simple bone cyst, bone metastasis, and osteosarcoma) and three soft tissue tumors (fibrosarcoma, two cases of cavernous hemangioma) that showed fluid-fluid levels on MR, and investigated their cause. Causes included blood in the cystic spaces, hemorrhage in the tumor, the telangiectatic component of the osteosarcoma, and the cavernous component of the hemangioma. No specific diagnosis could be made based on the finding of fluid-fluid levels. We conclude that fluid-fluid levels on MR are rather nonspecific findings in bone and soft tissue tumors and that the diagnosis should be made on the basis of other radiological and clinical findings.

Adult↗

PubMedDate not supplied

[Correlation between amniotic fluid index (AFI) and glucose concentration in amniotic fluid, glycaemia, glycosylated haemoglobin concentration during normal pregnancy and pregnancy complicated by insulin-dependent diabetes mellitus].

INTRODUCTION: In spite of confirming an association between type I diabetes of pregnancy with hydramnios, in the most recent studies there is still no clarity about which of the maternal or foetal metabolic parameters linked with amniotic fluid volume play the most important role. AIM: To determine which of the maternal or foetal metabolic parameters has the closest association with amniotic fluid volume. MATERIAL AND METHODS: The research was performed in two groups: normal healthy pregnant women (n=43) and group of pregnant women with insulin-dependent diabetes mellitus (n=17) and their newborns. We measured: amniotic fluid index, glucose level in serum and in amniotic fluid, in newborn serum, HbA1c level in serum of women and newborns. RESULTS: We found statistically significant higher values of amniotic fluid index 16.3 +/- 5.2 cm (p<0.01), glucose level in amniotic fluid 2.7 +/- 0.3 mmol/l (p<0.01), serum glucose level before delivery 8.4 +/- 1.6 mmol/l (p<0.001), glycosylated haemoglobin in serum levels 7.9 +/- 0.4% (p<0.05), levels of glucose and glycosylated haemoglobin in serum newborns 3.4 +/- 0.3 mmol/l (p<0.05), 7.7 +/- 0.4% (p<0.001) in patients with insulin-dependent diabetes mellitus as compared with these values in the group of healthy parturient women and their newborn infants. Performed correlation study shows statistical correlation between amniotic fluid index and glycosylated hemoglobin serum levels in the group of women with insulin-dependent diabetes mellitus (p<0.05), t=0.56. CONCLUSION: We conclude that long hyperglycemia or increased HbA1c level have an important role in amniotic fluid index increase in patients with insulin-dependent diabetes mellitus.

Adult↗

PubMed1986

Presence of alpha-lactalbumin, epidermal growth factor, epithelial membrane antigen, and gross cystic disease fluid protein (15,000 daltons) in breast cyst fluid.

The frequency of gross cystic breast disease in premenopausal women and its possible association with increased breast cancer risk emphasize the importance of investigations relating to breast cyst fluid composition. In order to contribute to a better analysis of this medium, we have measured four proteins the presence of which in human milk was well documented. Breast cyst fluid specimens from 266 breast cyst disease patients were assayed and compared as to concentrations of alpha-lactalbumin, gross cystic disease fluid protein (GCDFP-15), epidermal growth factor (EGF), and epithelial membrane antigen (EMA). All the analyzed cyst fluids contained GCDFP-15, EMA, and EGF whereas alpha-lactalbumin was detected in only 14.2% of fluids assayed. Positive correlations were observed between GCDFP-15 and EMA concentrations (P less than 0.005), as well as GCDFP-15 and EGF concentrations (P less than 0.0005). The cyst fluid GCDFP-15 and EGF levels were higher when alpha-lactalbumin concentrations were below detection limits. This association was statistically significant for GCDFP-15 (P less than 0.03) and for EGF (P less than 0.001). These results suggest that GCDFP-15 and EMA could be the biochemical expression of apocrine metaplasia and epithelial hyperplasia, respectively, two histopathological features which characterize breast cystic disease. On the other hand, the occasional presence of alpha-lactalbumin in the cyst fluid would reflect the persistence of differentiated cells in the epithelium surrounding the cyst and would be inversely proportional to the degree of cellular proliferation. The omnipresence of EGF in the cyst fluid argues for the hypothesis of its production by the mammary gland. The highly significant relationship between GCDFP-15 and EGF levels in the medium remains to be elucidated but may be related to an androgen sensitivity in the breast epithelium surrounding the cyst.

Apolipoproteins↗

PubMed1977

Bacterial growth inhibition by amniotic fluid. VII. The effect of zinc supplementation on bacterial inhibitory activity of amniotic fluids from gestation of 20 weeks.

Twenty human amniotic fluids obtained from gestations of 20 weeks' duration supported bacterial growth. Nine of the 20 fluids could be made inhibitory by adjusting the phosphate to zinc ratios of the fluids to less than 200 mug per milliliter. These fluids contained the phosphate-sensitive bacterial inhibitor previously, but the fluids contained sufficient phosphate to inactivate the antibacterial system. The remaining 11 amniotic fluids did not contain the peptide component of the phosphate-sensitive bacterial inhibitor and could not be made inhibitory by adjusting the phosphate to zinc ratio to less than 200 mug per milliliter. The data obtained suggested synthesis of the peptide component may occur at a gestational age of approximately 20 weeks. The peptide may indirectly be detected in fluids by determining whether antibacterial activity is obtained when the phosphate to zinc ratio of the fluids is adjusted to less than 200 mug per milliliter.

Amniotic Fluid↗

PubMed1996

The effects of age on the composition of uterine fluid of broiler breeder hens and on maintenance of quality of fowl spermatozoa when stored in uterine fluid or in a synthetic medium.

We studied effects of aging of hens on some physio-chemical parameters of uterine fluid taken at 2 stages of the ovulatory cycle (12 and 18 h after the oviposition) and of uterine fluid properties as a semen diluent in vitro at 41 degrees C. The volume, pH, osmotic pressure, Na+, K+, Ca2+, glucose and total protein concentrations of uterine fluid were measured. The percentage of motile spermatozoa and the fertilizing ability of fowl semen diluted 1:9 in uterine fluid were tested throughout the laying cycle in meat-type hens and compared to those of semen diluted in synthetic medium M199. The results showed that the volume and the K+ and Ca2+ concentrations in uterine fluid decrease with the hens' aging, while osmotic pressure is significantly higher in older hens. But in spite of the composition differences, uterine fluids from hens of different ages have little influence as semen diluents either on sperm motility or on fertilizing ability. These observations suggest that the variations of the composition of uterine fluid with hens' aging does not contribute to the late seasonal decline in fertility observed during the later stages of the reproductive season in this species. In any case, uterine fluid is harmful to sperm quality in vitro, while M199 is an appropriate semen diluent at 41 degrees C. The high glucose concentration in M199, however, slightly decreases motility and the duration of the fertile period of spermatozoa.

Journal Article↗

PubMed2006

Phase behavior of dipolar fluids from a modified statistical associating fluid theory for potentials of variable range.

A statistical associating fluid theory for potentials of variable range to model dipolar fluids is presented. The new theory, termed the SAFT-VR+D equation (the statistical associating fluid theory for potentials of variable range plus dipole), explicitly accounts for dipolar interactions and their effect on the structure of the fluid. This is achieved through the use of the generalized mean spherical approximation (GMSA) to describe a reference fluid of dipolar square-well segments. Isothermal-isobaric and Gibbs ensemble Monte Carlo simulations have been performed in order to test the new theoretical approach. Predictions for the thermodynamic properties and phase behavior of dipolar square-well monomer and chain fluids, in which one or more segments are dipolar, are considered and compared with new computer simulation data. The results show that the equation of state in general provides a good description of the phase behavior of dipolar monomer and chain fluids. Some deviations are seen between the simulation data and theoretical predictions for monomer fluids with large dipole moments and for molecules composed of segments with dipoles in different orientations. This is addressed through the replacement of the GMSA by the linearized exponential approximation.

Journal Article↗

PubMed2003

Contamination of brake fluid by power steering fluid.

Analysis of a contaminated brake fluid revealed the contaminant to be power steering fluid. The sample received at the laboratory presented two immiscible phases, which were both organic. Both fluids were analyzed by GC-MS and compared with a library of known automotive fluids. The bottom layer (brake fluid) consisted of glycols while the top layer (power steering fluid) consisted of aliphatics ranging from C16 to C25+. An interesting phenomenon of diffusion of some of the glycols from the brake fluid phase into the power steering fluid phase was observed and needs to be taken into consideration in the interpretation of the results.

Journal Article↗

PubMed1982

Effect of histamine and bradykinin on lymph and tissue fluid composition in the rabbit hindlimb: evidence for two compartments in tissue fluid.

In the rabbit hindleg lymph and tissue fluid were collected before and during close intraarterial infusions of histamine (2.5 and 10 micrograms/min) or bradykinin (0.4 microgram/min). Protein concentration was higher in the tissue fluid than in the lymph. During the 2h of the experiment in the control animals protein concentration increased in the lymph and decreased in the tissue fluid. Lymph flow decreased in the controls and it was not significantly influenced by histamine but was in the majority of animals (14 of 20) grossly augmented by bradykinin. Lymphatic protein and LDH fluxes were increased both by bradykinin and histamine. In animals where the vasoactive agents failed to alter lymph flow LDH concentration in the lymph increased significantly without comparable changes in the tissue fluid. On the other hand, in experiments where lymph flow was increased lymphatic LDH activity decreased but the activity in tissue fluid remained unchanged. It is concluded that the interstitial fluid is composed of two compartments. Only the perivascular compartment of tissue fluid is drained directly by the lymph vessels and lymph is essentially a recent microvascular filtrate. The second compartment of the interstitial fluid in rinsing the cells and connective tissue fibres, it is parallelly coupled and in exchange with the perivascular compartment.

Animals↗

PubMed1996

[Ileal occlusion with strangulation: importance of ultrasonography findings of the dilated loop with intraluminal fluid-fluid resulting from sedimentation].

To identify some dynamic or morphological patterns for the diagnosis of small bowel obstruction by hernia, adhesions or volvulus, we submitted to ultrasonography (US) 61 patients with clinical and radiographic syndromes of mechanical small bowel obstruction. The cause of obstruction was demonstrated in 58 patients-namely, with surgery in 56 patients and further instrumental examinations in 2. Three patients died before surgery. In our series, small bowel obstruction with strangulation was demonstrated at surgery in 31/56 patients. In 27/31 patients, US showed the coexistence of dilated loops with different kinetic behavior in the abdominal cavity-i.e., the simultaneous presence of akinetic loops with an intraluminal fluid-fluid level by sediment and peristaltic loops with solid particles in suspension. In 4/31 patients with strangulating small bowel obstruction. US provided no useful elements to explain the mechanism of obstruction. The US pattern of fluid-fluid levels due to intraluminal sediment in all the intestinal mass above the occluded segment was observed in 6/6 patients with uncompensated mechanical intestinal obstruction. This sign is due to the absence of intestinal muscular activity in the late stages of mechanical intestinal obstruction; its value is purely prognostic. To conclude, the US pattern of fluid-fluid levels by sediment diffused in the whole intestinal mass provides no useful elements to explain the cause of obstruction: on the contrary, the US pattern characterized by isolated intraluminal fluid-fluid levels or by the association of akinetic and peristaltic loops in the abdominal cavity appears pathognomonic of strangulating obstruction caused by volvulus, adhesion or hernia, with 87% sensitivity and 100% specificity.

Female↗

PubMed1976

Role of tissue fluid hyperosmolality in the pathogenesis of post-traumatic fluid loss.

The changes in interstitial fluid osmolality in the injured tissues during and after bilateral hind-limb ischaemia have been studied in young rabbits. There was a marked tissue fluid hyperosmolality which was directly related to the period of ischaemia. The increase in osmolality could be attributed to increases in lactate and potassium concentration. The interstitial fluid hyperosmolality disappeared rapidly after restoration of the circulation to the hind-limbs and would therefore only be of importance in the earliest stages of the pathogenesis of post-ischaemic fluid loss. Although the tissue fluid hyperosmolality is so short-lasting 56% of the fluid loss, measured 1 h after a 4-h period of ischaemia, occurs during the first 10 min following removal of the tourniquets. The lactate concentration in ischaemic skeletal muscle of the newborn rabbit was higher than that in the older animal and if this is reflected in an increased tissue fluid hyperosmolality it may be a factor in the greater post-ischaemic fluid loss characteristic of the neonate.

Age Factors↗

PubMed1995

Relaxin levels in amniotic fluid, extraembryonic coelomic fluid and maternal serum in early human pregnancy.

Separately identified samples of amniotic fluid and extraembryonic coelomic fluid together with maternal serum were collected from 22 women between 8 and 11 weeks of pregnancy and analysed for relaxin by immunoassay. Relaxin levels in maternal serum (median 1085 pg/ml; range 390-1259 pg/ml) were substantially higher than those in extraembryonic coelomic fluid (median 57.5 pg/ml; range 17-145 pg/ml; P < 0.0001; Mann-Whitney U-test). In turn, the levels of relaxin in coelomic fluid were higher than those in amniotic fluid (median 10 pg/ml; range 10-37 pg/ml; P < 0.0001; Mann-Whitney U-test). A linear correlation was found between relaxin levels in maternal serum and coelomic fluid (r = 0.68; P = 0.001) but there was no relation between levels in the other fluid compartments.

Amniotic Fluid↗

PubMed1997

Modelling the volume of expandable body fluid spaces during i.v. fluid therapy.

We have developed mathematical models to represent the changes in volume of fluid spaces associated with i.v. administration of a crystalloid solution. Input data for parameter estimations were dilution of blood, measured as reduction of blood haemoglobin concentration. The models were based on the assumption that the body strives to maintain volume homeostasis of fluid spaces and that the rate of restoration is a function of deviation from resting volume. Two models were derived; the first had a single fluid space into which fluid was administered and from which fluid left, the other model had a second fluid space communicating with the first. These models may be useful in the description and analysis of the effects of i.v. fluid therapy.

Adult↗

PubMed1994

Concentrations of vitamins A and E in amniotic fluid, extraembryonic coelomic fluid, and maternal serum in the first trimester of pregnancy.

Paired samples of amniotic fluid and coelomic fluid were obtained by transvaginal ultrasound guided amniocentesis from 15 women with an ultrasonographically normal pregnancy between 8 and 12 weeks' gestation. Vitamins A and E were measured in the two pregnancy fluids and in maternal serum by high performance liquid chromatography with detection by ultraviolet absorption and fluorimetry respectively. Concentrations of vitamins A and E were higher in maternal serum than in coelomic fluid and were generally undetectable in amniotic fluid. All differences in concentration were significant. The vitamin E/cholesterol ratio was similar in maternal serum and coelomic fluid. No correlation was shown between the vitamin concentrations in the three fluids. These findings suggest that the coelomic cavity plays a part in the maternofetal exchange of these vitamins.

Amniotic Fluid↗

PubMed1992

First-trimester amniotic fluid and extraembryonic coelomic fluid acetylcholinesterase electrophoresis.

Acetylcholinesterase (AChE) gel electrophoresis was performed on samples of amniotic fluid and extraembryonic coelomic fluid obtained by high resolution transvaginal ultrasound-guided amniocentesis from 38 women between 8 and 12 weeks of pregnancy. AChE was positive in 33 per cent (12/36) of the amniotic fluid samples; the percentage of positive results decreased as gestation advanced. AChE was positive in 32 per cent (9/28) of the extraembryonic coelomic fluid samples. In 81 per cent (21/26) of matched samples, the AChE results were identical in the two fluids. Amniotic fluid and extraembryonic coelomic fluid AChE electrophoresis cannot be used to diagnose neural tube defects prior to 12 weeks of gestation.

Acetylcholinesterase↗

PubMed1999

Hormonal effects on amino acids and related compounds in plasma, amniotic fluid, and allantoic fluid of the chicken embryo.

So far, more than 40 free amino acids and related compounds have been identified in plasma, amniotic fluid, and/or allantoic fluid of the 13-day chicken embryo. Concentration differences, and greatly varying behavior of these compounds under experimental conditions, revealed the presence of specific barriers among the three fluids. We tested the hypotheses that (1) the absence of an innervation of amnion and allantois indicates a hormonal control of their barriers, and (2) changes in the concentrations of certain amino compounds in the three fluids indicate anabolic or catabolic actions of hormones. Insulin, prolactin, and stress caused complex changes of the concentrations of amino compounds in all three fluids within 30 min. Some of these changes indicated breakdown of embryonic tissues, while others must have been due to transfer of amino compounds among the three fluid compartments. However, there was no significant effect on the glucose concentration in any of the three compartments under any of the experimental conditions. This is the first demonstration of hormonal effects on the amino compounds in the extraembryonic fluids of nonmammalian amniotes.

Allantois↗