Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “FLIES”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 127 records · Page 7Linked to original sources

"Not flying solo": phylogenetic identification and life-cycle insights of larval cestodes in the European flying squid Todarodes sagittatus (Cephalopoda: Ommastrephidae).

The European flying squid Todarodes sagittatus Lamarck is a widely distributed ommastrephid cephalopod in the Northeast Atlantic and Mediterranean Sea, yet its parasite fauna remains poorly documented in the Mediterranean Sea. In this study, two cestode larvae species infecting T. sagittatus from the Algerian coast (Western Mediterranean) were investigated using an integrative approach combining morphological observations and molecular analyses. A total of 63 squids were examined for parasitic infection. Cestodes in plerocercoid stage were detected in 31 individuals (prevalence of 46%), primarily located within the gastrointestinal tract, including the stomach, intestine, and caecum. Morphological features of the larvae were consistent with members of the order Phyllobothriidea but did not allow identification to the species level. Molecular analysis of the D1-D3 region of the 28S rDNA revealed two distinct larval lineages belonging to the genus Crossobothrium Linton, 1889. One lineage showed 100% sequence identity with the adult cestode Crossobothrium dohrnii (Oerley, 1885), a parasite of hexanchid sharks, confirming the identity of these larvae as plerocercoids of C. dohrnii. The second lineage clustered within the Crossobothrium clade but could not be assigned to a known species and is therefore referred to as Crossobothrium sp. The occurrence of these larvae in T. sagittatus, together with ecological data on squid diet and predator-prey relationships, suggests that this cephalopod acts as an intermediate or paratenic host in the life cycle of hexanchid cestodes. Infection likely occurs through predation on crustaceans acting as first intermediate hosts, while transmission to definitive hosts occurs when infected squids are consumed by sharks. These findings provide the first molecular identification of cestode larvae from T. sagittatus in the Mediterranean and highlight the important role of ommastrephid squids in the trophic transmission of elasmobranch parasites in pelagic ecosystems.

Crossobothrium↗

The house fly aliesterase gene (MdalphaE7) is not associated with insecticide resistance or P450 expression in three strains of house fly.

It was recently proposed that a mutation (G137D) in the MdalphaE7 gene was responsible for increasing transcription of a P450 (CYP6A1) resulting in resistance to diazinon. To examine if MdalphaE7 had a role in resistance in other strains we sequenced a fragment (approximately 700 bp) of the MdalphaE7 gene from individual flies of two insecticide susceptible and three insecticide resistant (due to increased monooxygenase-mediated detoxification) strains. Five unique alleles were discovered. While all of the susceptible strains had Gly137, so did the resistant LPR and NG98 strains. Of the two alleles in the YPER strain one had the G137D substitution and the other did not. Based on the lack of correlation between the presence of the 'mutant' MdalphaE7 and resistance (or P450 levels), we conclude that the G137D mutation in MdalphaE7 is not involved in transcriptional control of the P450s involved in resistance in the LPR, NG98 or YPER strains. The relationship between MdalphaE7 alleles and insecticide resistance is discussed in light of these findings.

Amino Acid Sequence↗

'Am not I a fly like thee?' From genes in fruit flies to behavior in humans.

The fruit fly Drosophila melanogaster has provided insight into the role of genes in behavior, some of which have relevant implications for humans. Mutants induced or engineered in the laboratory have contributed to our understanding of biological rhythms, learning, memory, neurodegenerative disease and drug response. Studies of naturally occurring genetic variation in behavior have advanced our understanding of what kinds of variants arise spontaneously and contribute to behavior.

Animals↗

Effects of the horn fly (Diptera: Muscidae) on physiological and nutritional responses of beef steers: continuous fly population levels.

Twenty-four Hereford-Angus crossbred beef steers were exposed to 0, 75, 150, and 225 horn flies, Haematobia irritans (L.), per head under controlled environmental conditions. The physiological and nutritional indices of all steers were recorded during a 14-d infestation period. Overall rectal temperature increased in steers exposed to 225 H. irritans was per head. Feed intake and nitrogen consumption by steers exposed to 225 H. irritans was lower than uninfested steers during the first 4 d. Overall serum cortisol was lower in steers exposed to 150 or 225 H. irritans per head compared with unexposed steers. Compared with unexposed steers, packed cell volume (percentage) was lower in steers exposed to 150 H. irritans on days 4, 7, and 14, lower in steers exposed to 225 H. irritans on days 7 and 14, and lower in steers exposed to 75 H. irritans on day 14. Blood urea nitrogen (BUN) was higher on day 1 in steers exposed to 75 H. irritans per head compared with uninfested steers, whereas BUN was lower in 225 H. irritans-exposed steers on days 7, 10, and 14 when compared with uninfested steers. Our data, in conjunction with previously published data, suggest that rectal temperature increases in beef steers exposed to > 150 H. irritans per head, and water consumption and urine production increases at infestation levels > 225 H. irritans per per head. Our data were unable to resolve H. irritans effects on steer heart rate, respiration rate, urinary nitrogen excreted, nitrogen retained, and serum cortisol levels.

Animal Nutritional Physiological Phenomena↗

The primary structure of the hemoglobin from the grey-headed flying fox (Pteropus poliocephalus) and the black flying fox (P. alecto, Megachiroptera).

The primary structures of the hemoglobins of two Flying Foxes of the genus Pteropus are presented. Both comprise two components: in P. alecto hemoglobin two alpha-chains at a ratio of 1:1 and two beta-chains at a ratio of 4:1 were detected. The hemoglobin of P. poliocephalus comprises one alpha-chain and two beta-chains, the latter at a ratio of 1:1. The globin chains were separated by high-performance liquid chromatography and the sequences determined by automatic liquid and gas phase Edman degradation of the chains and their tryptic peptides. Compared with human hemoglobin, the alpha-chains of P. alecto and P. poliocephalus show 18 and 19 exchanges, respectively, whereas in the beta-chains 16/17 substitutions are found in both cases. In the alpha-chains of P. alecto, one exchange involves an alpha 1/beta 1-contact. In the beta-chains of both species one heme-, one alpha 1/beta 2- and two alpha 1/beta 1-contacts are exchanged. The relevant side chains are the same in both species. The functional and systematic aspects of these findings are discussed.

Amino Acid Sequence↗

Trypanosomes in horse flies and deer flies in central New York State.

Tabanids were collected during 2 consecutive summers from 3 counties in New York using a canopy trap and insect net. Twenty-seven per cent of all fly specimens (N equals 641) representing 69% of the species collected (N equals 36) were infected with flagellates. Tabanid intestines harbored amastigote, choanomastigote, and epimastigote forms. Epimastigotes were frequently found, and trypomastigotes and choanomastigotes rarely found in cultures of tabanid intestinal flagellates. Epimastigote and trypomastigote forms closely resembled Trypanosoma theileri-like trypanosomes reported from ruminants.

Animals↗

A flying start to health promotion in remote north Queensland, Australia: the development of Royal Flying Doctor Service field days.

CONTEXT: The Royal Flying Doctor Service (Queensland Section), Australia, provides health services for remote populations across an area of approximately 600,000 km2. The challenge presented to the service was to provide effective health promotion as well as acute clinical services. ISSUE: This article describes the development of field days, an initiative based on a capacity building approach to health promotion. Field days have been conducted at station properties, national parks, remote tourist facilities, a roadhouse and country racecourse. They usually consist of a morning interactive workshop based on locally identified health topics followed by an afternoon clinic. A developmental approach has been adopted, with participant feedback from each field day and reflections by health staff informing future field day activities and adjustments in methods of delivery. Between November 2001 and August 2005, 726 adults and 248 children attended 53 field days conducted at 18 remote locations in north Queensland. EVALUATION: Participants were asked to rate the overall usefulness, content and quality of the presentations/workshops on a scale from 1 (not useful/poor) to 10 (very useful/very good). Overall, feedback from participants was positive. The average scores were 9.1 for overall usefulness of the day and content of the sessions, and 9.2 for quality of the presentations. Field days demonstrate a flexible health promotion initiative based on a capacity building approach which is rated as useful and appropriate by participants. LESSONS LEARNED: The implementation of field days has highlighted the importance of building on community and organisational strengths as a means of overcoming skepticism about new services and approaches to health. Methods of delivery that are fun, non-threatening, practical, locally relevant, culturally appropriate and interactive work best. The development of field day packages containing workshop plans and resources facilitates flexibility and enables the program to be tailored to meet the needs of participants. Workforce planning and development have played a key role in reorienting existing services towards health promotion. Field days can inform the development of locally relevant health promotion initiatives based on a capacity building approach in other remote areas of Australia and internationally.

Adult↗

Studies on flies in Korea. III. Taxonomic studies on muscid flies (Diptera, Muscidae).

Forty species belonging to 14 genera of the family Muscidae were found so far in Korea. They are described in this paper and 5th sternites, forceps and male genitalia are illustrated. Eleven species recorded by Kobayashi (1918-1940) and 16 species by Hori (1952) were revised and redescribed in this paper. The key to the species of the muscid flies in Korea are also presented.

Animals↗

Studies on medically important flies in Thailand. VI. Reports on 48 species of sarcophagid flies, including the taxonomic keys (Diptera: Sarcophagidae).

Flies from different geographical parts of Thailand were collected to study their medical importance as well as to make a zoogeographical study. After examination, 48 species of 19 genera, Kanomyia, Hosarcophaga, Blaesoxipha, Bercaea, Boettcherisca, Fengia, Lioproctia, Parasarcophaga, Phallosphaera, Rosellea, Sarcorohdendorfia, Sarcosolomonia, Sinonipponia, Pierretia, Thyrsocnema, Phytosarcophaga, Harpagophalla, Seniorwhitea, and Leucomyia belonging to Sarcophagidae were identified and those are reported in this paper. This study is based on the specimens available in the authors' collection.

Animals↗

Studies on medically important flies in Thailand VII. Report on 42 species of calliphorid flies, including the taxonomic keys (Diptera: Calliphoridae).

The calliphorid flies collected from different localities in Thailand were examined to study their medical importance as well as to study from the zoogeographical standpoint of view. Fourty-two species of 15 genera, Bengalia, Caliphora, Catapicephala, Chrysomya, Hemipyrellia, Hypopygiopsis, Lucilia, Melinda, Onesia, Phumosia, Pollenia, Polleniopsis, Tainanina, Tricycleopsis and Verticia belonging to both the subfamilies Calliphorinae and Chrysomyinae were found in Thailand. Many species are newly recorded from this country. The little known species, which are redescribed in detail with the illustration of the male genitalia and female ovipositors, and the key, which is revised to the genera and species, are also presented in this paper.

Abdomen↗